科尔沁沙质放牧草地植物多样性及生态位的分异规律研究

研究结果表明:①不同放牧强度下群落演替方向和速度有很大差异,其中过牧使植被种类组成趋于简单,植物多样性指数下降,草地中以一年生劣质牧草占绝对优势,轻牧和禁牧则相反;②由于群落的物种组成和植物种群数量的变化,使群落占据的物种生态位也明显分异,其中轻牧和禁牧的生态位宽度指数趋于增大,过牧趋于下降,中牧年际波动大。由于生态位分离明显,使生态位相似性降低,生态位重叠部分很小;③随着植被的分异,在属性空间上其位置发生飘移,变异越大,飘移的愈分散,其中轻牧和禁牧下植被在属性空间的位置呈曲线型分布,说明其演替呈有规律的渐变过程,而重牧下植被分异剧烈,各点在空间分布零散且距离远,说明其演替跃变性强。     

基于近缘EST-SSR引物的企鹅珍珠贝养殖和野生群体的遗传标记

利用近源物种马氏珠母贝的EST-SSR引物对企鹅珍珠贝DNA进行扩增,获得7对可扩增出目标条带的近缘分子标记,分析野生群体(YS)和养殖群体(YZ)企鹅珍珠贝的遗传多样性。结果显示,7个位点共获得26个等位基因;野生和养殖群体的等位基因数分别为3~5和3~4,平均期望杂合度(He)分别为0.623 5、0.496 8;平均观测杂合度(Ho)分别为0.431 8、0.302 8;HNUPM047位点的多态信息含量较低,为0.446 7,其他位点均在0.5以上,说明该遗传标记可提供比较丰富的遗传信息。表明养殖群体遗传多样性水平低于野生群体。     

自适应遗传算法配准三维数字样品模型及其CAD模型

通过包围盒的初始匹配和浮点数编码自适应遗传算法的精确匹配,实现了三维数字样品模型及其CAD模型的配准。初始匹配决定精确匹配空间变换参数的范围:精确匹配在搜索变换矩阵的6个变换参数过程中,采用最小二乘方法构造目标函数;遗传算法中引入种群多样性指标;在遗传操作的交叉算子和变异算子中引入该指标,并将该指标用于交叉概率和变异概率两个参数的调整,有效地克服了遗传算法的早收敛问题,实验结果表明该方法较好地解决了三维数字样品模型及其CAD模型的配准问题,且算法稳定,易于实现。     

山东日照和福建厦门沿海花鲈(Lateolabrax japonicus)遗传多样性的RAPD研究

采用RAPD技术 ,分析了日照、厦门沿海花鲈两群体的遗传多样性水平。结果表明 ,31条随机引物共扩增出两地花鲈 45 5条RAPD标记带 ,其中日照群体占 2 63条 ,厦门群体为1 92条 ;45 5条中有 1 84条带为两群体共有。日照群体内遗传相似度I为 0 892 8(差异度为0 1 0 72 ) ,厦门群体I为 0 92 2 5 (差异度为 0 0 775 ) ,而两者群体间的遗传相似度I为 0 8338(遗传距离D =0 1 662 )。两地花鲈在基因组DNA水平上存在较大的遗传差异。利用随机引物S1 1、S42、S45、S5 2和S2 0 3,在花鲈两群体间扩增出 8条稳定、明显的群体间特异性标记带     

广西英罗港红树林区鱼类多样性研究

1999年4个季度研究了广西英罗港红树林区的林缘和潮沟潮水中鱼类的种类多样性。共发现有鱼类76种,隶属于36科59属。其中林缘有42种,潮沟有54种,4个季度出现的鱼类种数潮沟多于林缘。两生境种类相似性指数较低,表现为：春季＞冬季＞夏季＞秋季。各季节均出现的鱼类在林缘有6种,占全年总种数的14．3％;潮沟有8种,占全年总种数的14．8％;两个季节间共有种最多的：在林缘是夏季与冬季,在潮沟是夏季与秋季,它们的相似性指数也最大。全年在林缘和潮沟均出现的鱼类仅有1种。本区鱼类以暖水性种占绝对优势;但潮沟暖温性种多于林缘。在生态类型上,林缘以中上层种类较多,潮沟则以底层种类较多。     

http://www.sciencedirect.com/science/article/pii/S1674987114000668

Microbial diversity in the hydrate-containing (sites SH3B and SH7B) and-free (sites SH1B, SH5B, SH5C) sediments collected from the Shenhu area of the South China Sea (SCS) was investigated using 16S rR...     

黄颡鱼(Pelteobagrus eupogon)不同生态地理分布群体遗传多样性的微卫星分析

利用40个黄颡鱼微卫星分子标记对吉林省月亮湖野生黄颡鱼(YL,30尾)、四川野生黄颡鱼(SC,30尾)、黑龙江省哈尔滨市松花江段黄颡鱼(SH,18尾)、湖北省荆州市长湖野生黄颡鱼子代(YY,30尾)及其亲本(QQ,30尾)、天津市换新国家级原良种场黄颡鱼1号(TJ,30尾)6个黄颡鱼群体的观测杂合度(Ho)、期望杂合度(He)、多态信息含量(PIC)和有效等位基因数(Ae)等进行了遗传检测,根据基因频率计算遗传相似系数和Nei氏标准遗传距离,x2检验估计Hardy-weinberg平衡,采用近交系数(FST)和基因流(Nm)分析群体的遗传分化及其来源.同时,使用PHYLIP3.63软件绘制基于Nei氏标准遗传距离的UPGMA进化树.6个群体共检测到5042个扩增片段,长度在150-650bp,40个基因座扩增出等位基因数从1-8个不等,共计143个等位基因,平均每个基因座扩增得到3.575个等位基因.结果显示:(1)6个黄颡鱼群体的多态性指标均适中,PIC在0.00-0.79之间,平均值为0.40、0.37、0.35、0.39、0.35和0.19,有效等位基因数(Ae)1.00-5.44个不等,平均有效等位基因数依次为2.05、2.30、1.93、2.07、1.93和1.47,无偏期望杂合度(He)在0.00-0.83之间,平均值为0.45、0.46、0.36、0.46、0.41和0.23;(2)遗传相似系数YY与QQ遗传相似度最高(0.9947),TJ与SC遗传相似系数最低(0.4846),聚类结果与地理分布呈一定相关性.     

The concept of ecological succession applied to phytoplankton over four consecutive years in five ponds featuring a salinity gradient

The distribution of phytoplankton abundance, biomass and species composition coupled with environmental factors and metazooplankton was studied relatively intensively and over a period of four consecutive years in five ponds featuring a gradient of increasing salinity from near to that of sea water to a nine-fold concentration from 2000 to 2003. The results indicate that the physical characteristics of the water (temperature and salinity) were quite similar over the years. Nutrients, which were concentrated in pond A1, decreased with increases in salt concentration. The composition of the phytoplankton community showed strong seasonality. Diatoms dominated in the first ponds A1, A16 and C2-1, followed by dinoflagellates. Chlorophyceae dominated the phytoplankton community in the hypersaline ponds M2 and TS. Cyanobacteriae were relatively abundant in ponds M2 and TS. The highest phytoplankton density and biomass were found in the ponds with the highest salinity due to the proliferation of Dunaliella salina (Chlorophyta: Volvocales). The inter-annual study of phytoplankton succession in the Sfax solar salterns showed slight differences among the years of study due to the stability of the environmental conditions. Phytoplankton communities were permanently primitive, stage 1 – structured as they failed to build complexity because of salt stress which operates for longer and above any other variables. This reduced frequency of disturbance to the existing course of regulation, allowed the community to “mature” from its “primitive” state, rather than experience frequent structural setbacks.     

Genetic diversity and population structure of Eleutheronema rhadinum in the East and South China Seas revealed in mitochondrial COI sequences

Eleutheronema rhadinum is a potential commercial fisheries species and is subject to intense exploitation in China. Knowledge on the population structure of E. rhadinum in Chinese coastal waters, which is important for sustainable exploitation and proper resource management, is lacking. In the present study, the genetic diversity and population structure of E. rhadinum were evaluated using a 564-base pair fragment of the mitochondrial cytochrome c oxidase subunit I （COl） gene. A total of 76 specimens were collected from three localities around the East （Qidong and Zhoushan） and South China Seas （Zhuhai）. Among these individuals, nine polymorphic sites were detected and 1 l distinct haplotypes were defined. High levels ofhaplotype diversity （h=0.759i0.035） and low levels of nucleotide diversity （re=0.001 98i0.003 26） were observed in these populations, Hierarchical analysis of molecular variance （AMOVA） indicated that 96.72% of the genetic variation occurred within the populations, whereas 3.28% occurred among populations. No significant genealogical branches or clusters were recognized on the neighbor-joining tree. Intra-group variation among populations was significant （~0~t=0.032 85, P〈0.01）. These results suggest that E. rhadinum populations in the East and South China Seas have developed divergent genetic structures. Tests of neutral evolution and mismatch distribution suggest that E. rhadinum may have experienced a population expansion. The present study provides basic information for the conservation and sustainable exploitation of this species.     

塔里木河下游物种多样性与地下水位灰色关联分析

根据植被样地资料和地下水埋深观测资料, 运用灰色关联法对物种多样性与地下水埋深的关系进行了分析. 结果表明: 在塔里木河下游, 物种多样性与地下水埋深变化关系密切, Shannon-Weiner指数、 Simpson指数、 Margalef指数、 Menhinick指数、 JSW指数和JSI指数与地下水埋深之间的灰色综合关联度都在0.70以上, 其中物种多样性指数Simpson指数与地下水埋深变化的灰色综合关联度最高, 为0.866, 其关联度数值体现了地下水埋深对物种多样性变化的主导性.