鳜鱼(Siniperca chuatsi)脂肪酸去饱和酶和延长酶基因全长cDNA序列的克隆与分析

利用RT-PCR和RACE方法克隆得到鳜鱼肝脏中控制高不饱和脂肪酸合成的脂肪酸去饱和酶(fatty acid desaturase,FAD)和脂肪酸延长酶(fatty acid elongase,ELO)基因的全长cDNA序列。FAD基因的全长cDNA序列1882bp,编码445个氨基酸,该蛋白序列含有FAD全部的特征结构区,包括3个组氨酸簇、2个跨膜区和1个细胞色素b5结构域,与其它鱼类FAD氨基酸序列的同源性为66.5%-90.1%,系统树分析显示其与欧洲鲈鱼和金头鲷等海水鱼类的亲缘关系最近。获得的ELO基因全长cDNA序列1401bp,编码294个氨基酸,该蛋白序列含有单一的氧化还原中心组氨酸簇、内质网停留信号和多个跨膜区等ELO特征结构,与其它几种鱼类ELO氨基酸序列的同源性为71.8%-86.7%,系统进化分析显示其与南方蓝鳍金枪鱼和金头鲷的亲缘关系较近。鳜鱼FAD和ELO基因全长cDNA序列的获得可为进一步研究其HUFA合成能力及调控机理奠定基础。     

Soldier crab (Dotilla myctiroides) distribution,food resources and subsequent role in organic matter fate in Ao Tang Khen,Phuket, Thailand

This study aimed to elucidate Dotilla myctiroides (H. Milne-Edwards) food resources and how the soldier crab influences the organic matter composition on the intertidal flat. A preliminary assessment of D. myctiroides size, sex, and population distribution was conducted to clarify the characteristics of the inhabiting population. Overall, D. myctiroides size and abundance was normal across the tidal flat area. Comparison of different sediment types from the soldier crab habitat (surface sediments, igloos, burrow lining, burrow pellets, feeding pellets, and feces) using the fatty acid (FA) analysis technique revealed D. myctiroides removes organic matter via the significantly lower contributions of polyunsaturated FAs, essential FAs, and dinoflagellates in the feeding pellets. Dotilla myctiroides tissue analysis of the hepatopancreas and hindgut in individuals that had been starved for 24 h confirmed not only the consumption of dinoflagellates, but diatoms and macroalgae as well. In addition, the FA metabolism within the digestive system implied the ability of the soldier crab to synthesize highly unsaturated FAs (HUFAs) such as 20:4n–6, 20:5n–3 and 22:6n–3.     

海洋产油真菌的简便初筛

选取分离自大洋、深海的28株酵母和143株霉菌,采用在培养基中添加尼罗红比较表观荧光强度的方法来筛选出酵母2株和霉菌7株,但菌体呈红色的酵母（17株）及菌丝为黑色的霉菌（28株）不适用于此法;通过单位DD鲫荧光强度的高低筛选出1株菌落为微红色的酵母,产油性能好于用第一种方法挑选出的2株酵母,且菌体颜色对结果并无影响,但只适合筛选酵母．通过上述2种方法筛选出高油脂菌株,大多来源于深海．     

王588块泥浆污染深穿透酸液研究与评价

王588块钻井泥浆污染主要是固相入侵和滤液与地层水的不配伍性。通过实验,优选出了具有高效深穿透能力以及固相溶解量大的表面活性剂DS-101酸液复配体系。实验表明,该体系耐温性强,对油泥具有极强的分散和溶解作用,对泥浆污染有明显的解堵效果,岩心渗透率恢复率可达127%。     

冷冻牡蛎贮存过程中脂溶性有毒物质的变化研究

牡蛎是我国出口量较大的水产品之一,由于产品本身的特殊性,在生长过程中易产生腹泻性贝毒(DSP).本实验中原料牡蛎DSP生物法检测结果为阴性,冷冻贮存4个月后检测结果即为阳性.本研究以分析这一变化原因为目的,采用LC-MS法对DSP生物检测的阳性样品进行确认,结果表明,生物法DSP检测的结果为假阳性,造成生物法DSP检测中小白鼠死亡的是牡蛎在贮藏过程中产生的脂溶性有毒物质.进一步分析确定该有毒物质为牡蛎脂肪水解物--游离脂肪酸.同时本研究对牡蛎在不同贮存温度和贮存时间下产生的游离脂肪酸含量进行了测定,在-10 ℃贮存温度下,贮存0,2,4,6,8,10个月,游离脂肪酸的变化范围为5.2～788.6 mg/kg;在-10 ℃,-18 ℃,-25 ℃贮存温度下,贮存4个月,游离脂肪酸的变化范围为92.5～389 mg/kg.     

Preparation and charaterization of self-assembled nanoparticles based on linolenic-acid modified chitosan

1 IntroductionHydrophobically modified water-soluble polymers,also known as hydrophobized polymers, are thosemodified by hydrophobic groups such as alkyl,arolkyl, and deoxycholic acid. In water, they canself-aggregate due to their intro- and/or intermolecularhydrophobic interaction. By this process, unique hy-drogel nanoparticles with hydrophobic core and hy-drophilic shell can be prepared, which is a new ap-proach to preparing the monodispersed hydrogelnanoparticles (Whitesides et al., 1991; …     

Explicit integration of a porosity‐dependent hydro‐mechanical model for unsaturated soils

An adaptive substepping explicit integration scheme is developed for a porosity‐dependent hydro‐mechanical model for unsaturated soils. The model is referred to as the modified σ –Θ model in this paper, which features the employment of the subloading surface plasticity and the stress–saturation approach. On numerical aspects, convex/nonconvex subloading surfaces in the σ –Θ space may result in incorrect loading–unloading decisions during the integration. A new loading–unloading decision method is developed here to solve the problem and then embedded into the explicit integration scheme for the modified σ –Θ model. In addition, to enhance the accuracy of the explicit integration, local errors from both hydraulic and mechanical components are included in the error control for each substep. A drift correction method is also developed to ensure the state point lies on the subloading surface in the σ –Θ space within a set error level. The performance of the loading–unloading decision method for the modified σ –Θ model is discussed through comparing it with the conventional loading–unloading decision method. The importance of involving the hydraulic component in the error control is also demonstrated. The accuracy and efficiency of the proposed adaptive substepping explicit integration scheme for the modified p–Θ model are also studied via several numerical examples. Copyright © 2016 John Wiley & Sons, Ltd.     

Consolidation in spatially random unsaturated soils based on coupled flow‐deformation simulation

This paper integrates random field simulation of soil spatial variability with numerical modeling of coupled flow and deformation to investigate consolidation in spatially random unsaturated soil. The spatial variability of soil properties is simulated using the covariance matrix decomposition method. The random soil properties are imported into an interactive multiphysics software COMSOL to solve the governing partial differential equations. The effects of the spatial variability of Young's modulus and saturated permeability together with unsaturated hydraulic parameters on the dissipation of excess pore water pressure and settlement are investigated using an example of consolidation in a saturated‐unsaturated soil column because of loading. It is found that the surface settlement and the pore water pressure profile during the process of consolidation are significantly affected by the spatially varying Young's modulus. The mean value of the settlement of the spatially random soil is more than 100% greater than that of the deterministic case, and the surface settlement is subject to large uncertainty, which implies that consolidation settlement is difficult to predict accurately based on the conventional deterministic approach. The uncertainty of the settlement increases with the scale of fluctuation because of the averaging effect of spatial variability. The effects of spatial variability of saturated permeability k_sat and air entry parameters are much less significant than that of elastic modulus. The spatial variability of air entry value parameters affects the uncertainties of settlement and excess pore pressure mostly in the unsaturated zone. Copyright © 2016 John Wiley & Sons, Ltd.     

Assessment of slope stability in cohesive soils due to a rainfall

The primary focus in this work is on proposing a methodology for the assessment of stability of natural/engineered slopes in clayey soils subjected to water infiltration. In natural deposits of fine‐grained soils, the presence of water in the vicinity of minerals results in an interparticle bonding. This effect cannot be easily quantified as it involves complex chemical interactions at the micromechanical level. Here, the evolution of strength properties, including the apparent cohesion resulting from initial suction at the irreducible fluid saturation, is described by employing the framework of chemoplasticity. The paper provides first the formulation of the problem; this involves specification of the constitutive relation, development of an implicit return mapping scheme, and the outline of a coupled transient formulation. The framework is then applied to examine the stability of a slope subjected to a prolonged period of intensive rainfall. It is shown that the water infiltration may trigger the loss of stability resulting from the degradation of material properties. Copyright © 2013 John Wiley & Sons, Ltd.