改良FS-3D-TrueFISP序列与传统序列在软骨成像中的对比研究

目的:总结正常软骨在改良脂肪抑制三维真实稳态自由进动序列上的表现,与传统序列对比研究软骨显像的图像质量,探讨经过改良以后的脂肪抑制三维真实稳态自由进动序列应用于软骨成像的可行性.方法:应用脂肪抑制三维快速小角度激发序列、三维双回波稳态序列、脂肪抑制快速自旋回波质子密度加权序列,以及经过改良以后的两个脂肪抑制三维真实稳态自由进动序列,对10名志愿者的膝关节进行扫描.测量各序列软骨及关节滑液的信号强度及背景噪声,计算各种方法所得图像的关节软骨的信噪比、关节软骨与滑液的对比噪声比及对比噪声比效率,比较各序列所得的信噪比、对比噪声比及对比噪声比效率之间有无统计学差异.结果:在改良的序列上软骨信号强度介于关节液与邻近骨质之间.6例信号均匀,4例软骨信号可见与脂肪抑制三维快速小角度激发序列相似的分层现象,各序列的信噪比、对比噪声比及对比噪声比效率差异具有显著性.采集次数为2次的脂肪抑制三维真实稳态自由进动序列的对比噪声比及对比噪声比效率优于其他序列.结论:经过改良后的真实稳态自由进动序列可以在保持关节软骨一定的信噪比的前提下在短时间内获得与关节滑液之间具有良好对比噪声比的图像.真实稳态自由进动序列可以用于关节软骨病变的诊断.     

真三轴条件下的原状黄土增湿变形特性

为了研究复杂应力条件下原状黄土的增湿变形特性，利用改进的非饱和土湿陷真三轴仪，在测量吸力条件下对原状黄土进行双线法分级浸水增湿试验，探讨力?水耦合作用下吸力的变化特性，研究真三轴浸水试验的增湿变形特性，以及吸力、净围压、中主应力和应力比对增湿变形的影响。研究结果表明：由ei-lgp压缩曲线确定了非饱和土的屈服点，屈服点之前，曲线的斜率近似相等；屈服点之后，曲线的斜率随着初始吸力的减小而减小，且非饱和土与饱和土的交点随着初始吸力的增大呈右下方移动趋势。等向压缩过程中，吸力随着净平均应力的增加近似呈线性减小趋势，初始吸力越小，吸力减小的速度越慢，且吸力变化量越小。均压分级浸水增湿时，吸力s随着浸水量w的增加而减小，增湿体变 随着浸水量w的增加而增大，不同净围压下的 -w关系曲线相互有交叉。交叉区域之前，净围压越大，增湿体变越小，而交叉区域之后，净围压越大，增湿体变越大。在同一净围压条件下，浸水量相同时，初始吸力越大，增湿体变越大。真三轴剪切增湿试验中，应力比k、中主应力参数b值和净围压?3皆对增湿变形有一定的影响。当q/p≤1时，增湿变形曲线前段下凹后段上凹，主要表现为体积增湿变形；当q/p>1时，增湿变形曲线只有下凹部分，主要表现为剪切增湿变形。增湿变形曲线的形态取决于k值和b值的不同组合。     

采样过程和模数转换对记录的影响及“真实地面运动”获取——地震学上用的数字信号处理的基本原理和实例(讲座3)

通过采样过程和模数转换对记录影响的分析,讨论了数字地震仪记录的动态范围,以及如何使用谱分析方法去除仪器对最终记录的影响,以便获取“真实地面运动”的近似。     

试用矢量表示岩层真厚度

在野外地质测量工作中,岩层真厚度历来使用标量计算。文中证明：如果规定在导线前进方向上,凡是自下部向上部测得的岩层真厚度为正厚度,反之则为负厚度,可以使用公式Ｍ＝Ｌ·［ｓｉｎβ·ｃｏｓε·ｃｏｓ（－λ）＋ｓｉｎε·ｃｏｓβ］进行岩层真厚度计算。该公式各参数的取值范围与野外实测剖面过程中各数值可能出现的范围相同,而且所有数据均可直接取于野外实测剖面数据记录表,不需要对数据作任何处理,计算过程简单准确。其计算结果不但可得出岩层的真厚度,而且还可真实地反映出岩层之间的上下关系,从而使岩层厚度具有矢量性质。     

Macrofauna along the Sudanese Red Sea coast: potential effect of mangrove clearance on community and trophic structure

Mangroves along the Sudanese Red Sea coast are under constant anthropogenic pressure. To better understand the influence of mangrove clearance on the intertidal benthic community, we investigated the composition, biodiversity and standing stock of the macrofauna communities at high‐, mid‐ and low‐water levels in three contrasting habitats: a bare sand flat, a cleared mangrove and an intact mangrove. In addition, a community‐wide metric approach based on taxon‐specific carbon and nitrogen isotope values was used to compare the trophic structure between the three habitats. The habitats differed significantly in terms of macrofaunal standing stock, community composition and trophic structure. The high‐ and mid‐water levels of the intact mangroves showed a distinct macrofaunal community characterized by elevated densities and biomass, largely governed by higher decapod and gastropod abundances. Diversity was similar for cleared and intact mangroves, but much lower for the bare sand flat. Community‐wide metrics indicated highest trophic diversity and community niche breadth in the intact mangroves. Differences between the cleared and intact mangroves can be partly attributed to differences in sediment characteristics resulting from mangrove clearance. These results suggest a significant impact of mangrove clearance on the macrofaunal community and trophic structure. This study calls for further investigations and management actions to protect and restore these habitats, and ensure the survival of this ecologically valuable coastal ecosystem.     

广西红树林根际土壤放线菌的原位培养分离及其活性筛选

为了发掘广西红树林根际土壤的放线菌资源,本文利用原位培养装置,埋于根际土壤中俘获放线菌,30d后取回实验室,采用平板涂布法对4个地点的原位培养样品于15种培养基上进行分离纯化;对分离株基于16SrRNA基因序列进行系统发育分析;进一步进行抗菌活性和产酶活性检测。共分离得到113株放线菌。对其中33株放线菌进行测序,结果表明20株属于链霉菌属,11株属于拟诺卡氏菌属,1株属于伦兹氏菌属,1株与拟诺卡氏菌属相似性最高为90%,很可能属于放线菌一个新属。抗菌实验结果显示其中有7株、4株、18株、6株、10株、3株实验菌株分别对大肠埃希菌、铜绿假单胞菌、金黄色葡萄球菌、普通变形杆菌、乙型溶血性链球菌和肺炎克雷伯氏菌具有抑制作用;有55株、62株、24株、72株的实验菌株分别具有纤维素酶活性、淀粉酶活性、胶原蛋白酶活性、酯酶活性。原位培养可以丰富对广西红树林根际土壤的认识,分离到了新种甚至可能是新属的放线菌,分离得到的部分放线菌菌株具有较高生物活性,为后续工作提供了良好的实验材料。     

广西钦州湾百年来红树林演变的有机碳同位素和孢粉示踪及其影响因素

气候变化和人类活动制约下的红树林演变是一种长时间尺度效应,而沉积物则是记录这种响应的最佳档案。采用古生态学研究思路,选择有效的示踪参数是解读这一档案的有效途径。本文通过广西钦州湾红树林区1根柱状沉积物中有机碳同位素(δ13C)、C/N分析和孢粉鉴定,以沉积物中的红树林源有机碳贡献和红树植物孢粉组合为示踪参数,在210Pb年龄框架构建的基础上,通过对比研究红树林源有机碳贡献和红树孢粉组合特征,揭示百年来钦州湾红树林兴衰和群落演替规律;进而重塑其红树林演变历史:兴盛期(1864-1918年)、衰退期(1918-1968年)和低谷期(1968-2007年),这与土地替代、遥感分析和现场勘测数据较为吻合。结合气候变化和人类活动资料,发现钦州湾红树林的近期衰退主要源于人类活动的影响,尤其是虾塘围垦;而与气候变化关系不大。     

Diet of Haplognathia ruberrima (Gnathostomulida) in a Caribbean marine mangrove

Haplognathia ruberrima is a cosmopolitan gnathostomulid species found in sulfur bacterial mats in mangroves in Guadeloupe (French West Indies). Haplognathia ruberrima presents a δ¹³C value lower than all measured meiofaunal grazers and lower than the available measured food sources of this environment. This low δ¹³C value can not be due to specific ingestion of ¹³C‐depleted methanogenic bacteria because abundances of those bacteria are reduced in surficial and deep sediments as revealed by δ¹³C of bacterial fatty acid. According to scanning electron microscope observations, no bacterial ectosymbionts were observed at the surface of the gnathostomulids, and transmission electron microscope views revealed the absence of bacterial endosymbionts. Energy‐dispersive X‐ray spectroscopy analysis detected low levels of sulfur (0.32%±0.8) in biological tissues of H. ruberrima, confirming the absence of thioautotrophic bacterial symbionts in these animals. Consequently, the low δ¹³C value of H. ruberrima can not be due to the presence of sulfur‐oxidizing symbionts but more probably to the selective and exclusive consumption of free‐living, sulfur‐oxidizing bacteria.     

An inflection in the rate of early mid-Holocene eustatic sea-level rise: A new sea-level curve from Singapore

This study presents a sea-level curve from 9500 to 6500 cal BP for the farfield location of Singapore, on the Sunda Shelf in southeast Asia. The curve is based on more than 50 radiocarbon dates from elevations of +1.43 m to −15.09 m representing sea-level index points in intertidal mangrove and shallow marine sediments deposited by sea-level rise accompanying deglaciation. The results indicate that mean sea level rose rapidly from around −17 m at 9500 cal BP to around −3 m by 8000 cal BP. After this time, the data suggest (but do not unequivocally prove) that the rate of sea-rise slowed for a period of 300–500 years centred on 7700 cal BP, shortly after the cessation of meltwater input to the oceans from the northern hemisphere. Renewed sea-level rise amounting to 3–5 m began around 7400 cal BP and was complete by 7000 cal BP. The existence of an inflection in the rate of sea-level rise, with a slow-down centred on 7700 cal BP, is broadly consistent with other available sea-level curves over this interval and is supported by evidence of stable shorelines and delta initiation elsewhere at this time, as well as evidence of comparatively rapid retreat of the West Antarctic ice sheet beginning around 7500 cal BP. ‘Stepped’ sea-level rise occurring shortly after 7500 cal BP and also earlier during deglaciation may have served to focus significant post-glacial episodes of human maritime/coastal dispersal, into comparatively narrow time intervals.     

三种红树根部组织抗氧化酶活性对水淹胁迫的响应

以自然种群高程分布为根据, 选择对潮汐浸泡耐性存在一定差异的白骨壤(Avicennia marina)、桐花树(Aegiceras corniculatum)和木榄(Bruguiera gymnorrhiza)为实验树种。对3种红树幼苗进行为期0d、1d、3d、5d、7d、14d的水淹处理, 测定其根部组织的过氧化物酶(peroxidase, POD)活性、超氧化物歧化酶(superoxide dismutase, SOD)活性、抗坏血酸氧化酶(ascorbic acid oxidase, APX)活性和过氧化氢酶(catalase, CAT)活性。水淹处理会导致植物根部组织受低氧胁迫从而破坏活性氧(reactive oxygen species, ROS)生产和消除的平衡, ROS大量累积给植物带来氧化损伤。实验测定与清除ROS有关的酶指标, 结果表明POD活性在1d至14d的水淹周期内随时间呈现上升趋势, 这可能与POD参与活性氧以及乙醇、乳酸等有毒物质的反应有关。CAT、SOD、APX活性均呈现应激上升后下降趋势, 由此推断该系列抗氧化酶在水淹胁迫发生前中期对消除红树植物根部组织大量产生的活性氧具有重要意义。抗氧化酶系在3种红树植物中的表达存在一定差异, 但整体仍呈相似的变化趋势。通过层次聚类法对各项酶活性指标分析后发现, 白骨壤抗氧化酶系的活性模式与木榄和桐花树的存在一定差异, 但没有发现木榄与桐花树抗氧化酶系统表达模式的明显差异。本研究旨在更好地了解红树林对水淹胁迫的生理适应机制, 为红树林生态系统保护与管理提供理论依据。