巢湖沉积植硅体组合及中全新世以来的环境演变

尽管以前对眼虫进行过大量的形态发育研究和基于核糖体RNA基因的系统发育分析,但对于株系之间的关系仍然知之甚少．因其形态特征有限并且易变,很难鉴定眼虫的相似种和同种内不同的株．作者利用微卫星DNA指纹图谱,在七株眼虫中扩增了七个微卫星DNA位点,成功扩增的六个微卫星引物都得到了四到八个条带．从微卫星DNA指纹图谱计算得到的相似性系数范围从0．000到0．957．根据相似性系数得到的树状结构,七株眼虫在距离为0．9346处分为三支:E．mutabilis,E．intermedia和E．gracilis．其中,五株E．gracilis分为两组:来自日本的和美国的．不同地区的株得到不同的基因型,并初步分析了它们之间的关系．研究表明七株眼虫根据微卫星DNA指纹图谱被明显区分开．微卫星DNA指纹图谱具有很高的分辨率,是鉴定和区分原生动物相似种和同种内不同株的一种有用的新方法．     

基于多格网算法的卫星图像恢复

将求解二次椭圆偏微分方程问题的多格网算法应用到卫星影像的恢复中。其基本思想是在将由总变分求极小生成的偏微分方程离散成为差分方程的过程中,采用不同大小的格网。在取得较好恢复结果的同时,提高了传统图像恢复算法的运算效率,降低了恢复运算所需要的时间。     

EM120型多波束测深系统及在深海测量中的应用

与传统的单波束回声测深仪相比,多波束测深系统具有水深全覆盖无遗漏扫测,测量范围大、速度快,测深精度和分辨率高,记录数字化和实时自动绘图等优点。介绍了EM120型多波束测深系统深水系统的技术性能,分析了EM120在中沙深海区的测量试验情况及在深海区水深测量中的应用价值,提出了存在问题及其对策。     

全站仪内供电系统的保养

全站仪是由电子经纬仪与光电测距仪组合的仪器,是集自动测距、测角、计算、数据存储及数据传输于一体的自动化、数字化及智能化的三维坐标测量与定位系统,是一种当今广泛应用于大地测量、工程测量、数字化测图的电子测量仪器,是现代测绘行业最主要、最普通的测绘仪器,仪器的维护和供电系统的保养,已经成为测绘生产单位的一项工作。     

华清4#泉水溶气体百分含量合计的强震远场前兆特征

华清4#泉水溶气体百分含量合计(QB)的取值范围为100±5.按4舍5入法取整数值,将其分为11个区段.以年为单位,统计QB落在每个区段内的频次.频率分布直方图显示,在QB取值区间内,分区段频率呈近似正态分布.将落入QB频率最高的区段称为轴,将轴在不同区段上位置的变化称为轴漂.研究表明,QB在不同区段上轴的漂移,反映了区域应力场的不同状态.轴落入99区段上,反映的是区域应力场的增强,与远场M≥7.0地震及伴生中强地震、鄂尔多斯块体周边后继中强地震有很好的相关性;轴落入98区段上,反映的是区域应力场的相对稳定;轴落入97区段上,反映的是巨大地震后,区域应力场的调整及中强地震活动格局的变化.文中还阐述了99区段轴值与地震对应关系的几个特征.     

Morphological and molecular evidence supports the first occurrence of two fishes,Siganus sutor(Valenciennes, 1835)and Seriolina nigrofasciata(Rüppell, 1829)(Actinopterygii:Perciformes), from marine waters of Odisha coast,Bay of Bengal,India

Marine ecosystems provide a wide variety of diverse habitats that frequently promote migration and ecological adaptation. The extent to which the geographic distribution of marine organism has reshaped by human activities remains underappreciated. The limitations intrinsic to morphology-based identification systems have engendered an urgent need for reliable genetic methods that enable the unequivocal recognition of fish species,particularly those that are prone to overexploitation and/or market substitution. In the present study, however, an attempt has been taken to identify two locally adapted fish species, Siganus sutor(Valenciennes, 1835) and Seriolina nigrofasciata(Rüppell, 1829) of order Perciformes, which happens to be the first record in Odisha coast,Bay of Bengal. The diagnostic characteristics of Siganus sutor are: dorsal fin XIII-10, anal fin VII-9, pectoral fin 15,pelvic fin II-3, while that of Seriolina nigrofasciata dorsal fin VI-I-35, anal fin I-17, pectoral fin 16, pelvic fin 5. All COI barcodes generated in this study were matched with reference sequences of expected species, according to morphological identification. Bayesian and likelihood phylogenetic trees were drawn based on DNA barcodes and all the specimens clustered in agreement with their taxonomic classification at the species level. The phylogeographic studies based on haplotype network and migration rates suggest that both the species were not panmitic and the high-frequency population distribution indicates successful migration. The result of this study provides an important validation of the use of DNA barcode sequences for monitoring species diversity and changes within a complex marine ecosystem.     

Feeding intensity and molecular prey identification of the mantis shrimp,Oratosquilla oratoria (De Haan, 1844), in the Tianjin coastal zone of Bohai Bay

Bohai Bay, a representative semi-enclosed bay of China, has been severely impacted in recent decades by rapid human development. Although the mantis shrimp Oratosquilla oratoria plays an important role in the Bohai Bay ecosystem and fishery, its feeding ecology and the impact of habitat changes on its feeding habits are poorly known. In this study, we sought to identify its feeding strategy through the separation of stomach contents, and to describe its trophic ecology during maturation in the Tianjin coastal zone of Bohai Bay by the DNA barcoding method using a fragment (about 690 bp) of the mitochondrial cytochrome oxidase I (CO I) gene. A total of 594 specimens were collected from March to July 2018, and 347 (58.42%) stomachs were found to have food remains. More than half of the O. oratoria individuals had poor feeding activity, and the degree of feeding of females was higher than that of males, but there was no significant difference in the visual fullness index and the fullness weight index (FWI) between sexes for each month. In addition, the feeding activity of O. oratoria was consistent across the months, suggesting in artificial breeding programs it is not worried too much about the accessional diet supplied to the female bloodstocks except maintaining their normal food. A total of 167 retrieved prey items yielded 187 readable sequences; 86.63% of these sequences could be assigned to the species level. Consequently, 25 different prey taxa covering all readable sequences were identified, showing O. oratoria is a generalist predator. In terms of percent composition by frequency of occurrences (%N), crustaceans and fishes accounted for the same and highest percentage (35.83%) in the mantis shrimp diet, followed by mollusks (24.59%) and the remaining marine organisms (3.73%) including Polychaeta (2.67%), Sagittoidea (0.53%), and Chromadorea (0.53%). The result shows the benthic fish Oxudercidae (family) appears to be an especially important food supply for O. oratoria during maturation. The degree of cannibalism decreased with an increase in diet species and the frequencies of cannibalism in this study was noticeably higher than that in previous studies, suggesting that cannibalism may be a significant feeding strategy in the mantis shrimp O. oratoria in the Tianjin coastal zone of Bohai Bay, and because of the frequently cannibalistic behavior, the high stocking density of broodstocks should be avoided.     

虾夷扇贝TET基因在配子发生和早期发育中的表达模式

DNA甲基化参与调节动物配子发生和胚胎发育过程,TET基因负责DNA主动去甲基化,在基因印迹去除和细胞全能性获得中起重要作用。为了解海洋贝类配子发生和胚胎发育过程中DNA去甲基化如何发生,本研究以虾夷扇贝为研究对象,鉴定了其TET基因,并分析了该基因在性腺和胚胎幼虫发育中的表达变化。结果表明,虾夷扇贝基因组中含有1个TET基因(PyTET),该基因长39127 bp,包含10个外显子,编码1592个氨基酸,其蛋白具有完整的2OGFeDO超家族的加氧酶结构域。在性腺发育过程中,PyTET基因表达峰值出现在休止期精巢和增殖期卵巢,原位杂交结果显示其在精原细胞、精母细胞中均有表达,在卵母细胞中的表达明显高于卵原细胞;在早期发育过程中,其峰值出现在囊胚期。以上结果提示虾夷扇贝配子发生和胚胎发育过程中均发生了DNA主动去甲基化,这将有助于系统了解表观调控在贝类发育中的作用。     

区域创新政策评价的框架、测度与检验

构建区域创新政策评价的理论框架,在宏观知识生产函数中引入信息化作为创新技术进步的替代变量,基于DEA与Malmquist指数,用全要素生产率指数存量来衡量区域创新政策。并以省际高技术产业为例进行了实证,研究结果表明：区域创新政策的特征和评价维度决定评价的多样性,基于政策效果与政策交互作用的区域创新政策评价具有重要意义;区域创新政策总体上处于稳步提高阶段,较低地区以西部中部地区为主;区域创新政策测度模型系统性较好,其检验方法有待进一步深化;采用政府研发经费投入作为区域创新政策的替代变量值得商榷。     

加权总体最小二乘的效率优化算法

加权总体最小二乘法是理论上估计EIV模型参数相对严密的方法,其迭代过程中涉及的矩阵运算较为耗时,在处理大量级数据时尤其明显。PEIV模型有助于提高加权总体最小二乘法的计算效率。本文基于PEIV模型和经典最小二乘准则给出了一种加权总体最小二乘法算法,算法的推导过程简洁,易于理解,迭代过程中无需重构矩阵,减少了矩阵运算量。最后通过仿真试验验证了算法的可靠性。试验结果表明,本文算法可以取得与现有算法相同的参数估计精度且计算效率更高。