苯并（a）芘诱导下岩虫CYP基因的表达特征

Rapid amplification of cDNA ends(RACE) and real-time polymerase chain reaction(RT-PCR) were carried out to analyze the CYP4 gene expression in polychaete Marphysa sanguinea exposed to benzo[a]pyrene(BaP) in this study. The full length of MsCYP4 cDNA was 2 470 bp, and it encoded 512 amino acids. The deduced amino acid sequence showed 47% identity with CYP4 F from frog Xenopus tropicalis and shared high homology with other known CYP4 sequences. To analyse the role of CYP4 in protecting M. sanguinea from BaP exposure, three BaP groups were established: 0.5, 5 and 50 μg/L. Polychaetes were sampled after 3, 7 and 12 d. At 0.5 μg/L, the effect of BaP on MsCYP4 gene expression increased with time prolonged. MsCYP4 gene expression curve showed Ushaped trend with time in 5 and 50 μg/L BaP groups. Therefore, MsCYP4 gene may play an important role in maintaining the balance of cellular metabolism and protecting M. sanguinea from BaP toxicity.     

大地水准面高的计算及其在海底地形反演中的应用

为充分挖掘海洋重力数据在反演海底地形中的应用潜力,尝试探索利用大地水准面高反演海底地形的技术途径,并以夏威夷—皇帝海山链拐点所在海区作为反演试验区进行验证。首先采用Belikov列推法计算伴随(缔和)勒让德函数,利用EIGEN-6C4地球重力场模型解算获取了分辨率为1'的大地水准面高格网数值模型;然后通过综合分析反演比例函数和转换函数特点、研究海区大地水准面高与海底地形的相干特性以及大地水准面高本身尺度特征,获得了利用大地水准面高反演海底地形的频段范围;最终以试验海区大地水准面高为数据输入,构建了相应的海底地形模型(BNT模型),并与ETOPO1等海深模型进行比对分析。试验结果表明:BNT模型检核差值在一倍均方差范围检核点数量占比70.60%,相比正态分布更加集中;BNT模型检核精度低于ETOPO1等海深模型;海深模型检核精度随着水深增加不断提升,水深小于1 000 m时,海深模型相对误差出现较大发散现象;计算海域ETOPO1模型精度最高,GEBCO模型和DTU10模型检核精度相当。     

差分层析SAR技术研究现状分析

差分层析SAR是在层析SAR基础上发展起来的一种四维信息反演技术。它不仅实现了对雷达目标的三维分辨能力,同时可以获取目标的形变速率信息,可实现对目标方位-距离-高度-时间四维成像,这对实现城市基础设施动态形变监测、古建筑风险评估、重要工程安全监测等应用具有重要实际意义和价值。本文基于差分层析SAR成像原理,分析了成像处理过程中存在的问题,总结了差分层析SAR成像算法研究现状和特点;最后列举了差分层析SAR技术的主要应用领域,并对其技术发展趋势进行了展望。     

Impacts of TRMM SRR assimilation on the numerical prediction of tropical cyclone

1 IntroductionObservation of the tropical rainfall is crucial forthe research on tropical weather and climate. Nu-merous studies have shown that the ingestion of rain-fall data into a numerical model can have considera-ble impacts on simulation results(Kr…     

氨氮胁迫对中华绒螯蟹(Eriocheir sinensis) 免疫功能的影响

在实验室条件下构建不同氨氮浓度环境和养殖时间的组间差异,研究氨氮胁迫对中华绒螯蟹免疫功能的影响。结果表明,中华绒螯蟹在NH 4-N3.0mg/L10天或1.0mg/L20天时,其血淋巴血细胞密度极显著地低于对照组(P<0.01)。NH4 -N2.0mg/L和3.0mg/L无论10天或20天,血细胞吞噬百分率均分别为显著(P<0.05)和极显著(P<0.01)地低于对照组。NH4 -N3.0mg/L10天或2.0mg/L20天,血细胞吞噬指数显著低于对照组(P<0.05)。NH4 -N1.0mg/L和2.0mg/L分别20天时,血清溶菌酶(LSZ)活力分别为显著(P<0.05)和极显著(P<0.01)地低于对照组。NH4 -N1.0—5.0mg/L10天时,血清酚氧化酶(PO)活力均极显著(P<0.01)下降;但NH4 -N1.0—4.0mg/L20天时,血清PO活力水平均比10天时有所上升。NH4 -N3.0—4.0mg/L10天或1.0—2.0mg/L20天,以及5.0mg/L10天或3.0—5.0mg/L20天时,超氧化物歧化酶(SOD)活力分别为显著(P<0.05)和极显著(P<0.01)地低于对照组。上述结果表明,随着氨氮胁迫浓度的增加和胁迫时间的延长,会引起中华绒螯蟹血细胞数量、血细胞吞噬能力、溶菌酶活力、酚氧化酶活力和超氧化物歧化酶活力等的逐渐下降,使机体非特异性免疫防御系统遭到损伤,同时机体清除自由基的能力下降,机体细胞和组织受到伤害甚至出现死亡。     

几种常用消毒剂对杂色鲍幼鲍的急性毒性试验

在常温静水的条件下,分别用高锰酸钾、菌毒净、聚维酮碘对杂色鲍(Haliotis diversi-color Reeve)幼鲍进行急性毒性试验。试验结果表明,毒性大小依次为高锰酸钾>菌毒净>聚维酮碘。高锰酸钾对幼鲍24,48 h的LC50分别为17.1,5.18 mg/L。菌毒净对幼鲍24,48h的LC50分别为105,46.5 mg/L。聚维酮碘对幼鲍24,48 h的LC50分别为530,218 mg/L。菌毒净、聚维酮碘是较为理想的消毒药物。     

区域性森林大火的真正成因

森林大火真正起因于地球排气中易燃还原性气体在腐殖层的临界积聚(气体体积分数大约为5%~6%)。现场气体(H2,CO)系统测量结果证实了这一点。测量结果显示,该林区腐殖层中H2、CO质量分数分别为(10~170)×10-6(本底值为0·5×10-6)和(2~60)×10-6(本底值为0·5×10-6),二者均远高于大气本底值数十到数百倍,而且未燃区的测值显然高于1998年燃区。     

华清4#泉水溶气体百分含量合计的强震远场前兆特征

华清4#泉水溶气体百分含量合计(QB)的取值范围为100±5.按4舍5入法取整数值,将其分为11个区段.以年为单位,统计QB落在每个区段内的频次.频率分布直方图显示,在QB取值区间内,分区段频率呈近似正态分布.将落入QB频率最高的区段称为轴,将轴在不同区段上位置的变化称为轴漂.研究表明,QB在不同区段上轴的漂移,反映了区域应力场的不同状态.轴落入99区段上,反映的是区域应力场的增强,与远场M≥7.0地震及伴生中强地震、鄂尔多斯块体周边后继中强地震有很好的相关性;轴落入98区段上,反映的是区域应力场的相对稳定;轴落入97区段上,反映的是巨大地震后,区域应力场的调整及中强地震活动格局的变化.文中还阐述了99区段轴值与地震对应关系的几个特征.     

Correspondence between the large volcanic eruptions and ENSO events over AD 1525–2000

ENSO is an interannual mode which may be affected by external forcing, such as volcanic eruptions. Based on the reconstructed volcanic eruptions chronology and ENSO sequences, both 195 large volcanic eruptions(VEI≥4) and 398 ENSO(El Ni?o and La Ni?a) events were extracted from 1525 to 2000. An analysis of the correspondence between the large volcanic eruptions and ENSO events was performed by matching the large volcanic eruptions with the types and magnitudes of ENSO events present in the 0–2 years after the eruptions. The results show the following:(1) The percentages of ENSO events within the 3 years after the large eruptions had increased to 68.3% from 31.7% compared with those with no-eruptions in the previous 0–2 years. In addition, the ratio of El Ni?o to La Ni?a events turned from 2:3 to 1:1, and more El Ni?o events occurred in the 0 year after eruptions in the low-latitudes of the Northern Hemisphere and in the tropics but more La Ni?a events occurred in the 0 year after in the high-latitudes of the Northern Hemisphere and the Southern Hemisphere.(2) After the eruptions, the weak(W) El Ni?o events had increased by 8 percentage points and the very strong(VS) El Ni?o events had decreased by 10 percentage points; conversely, there was a decrease by 15 percentage points of the weak La Ni?a events and an increase by 11.4 percentage points of the very strong La Ni?a events. Specifically, the percentages of strong La Ni?a events increased to a peak at 1(+1) year after the eruptions.(3) The percentage of eruptions followed by single-year ENSO was the greatest. The percentage of ENSO events that occurred in the consecutive 2 years following an eruption was approximately equal to the percentage of events that occurred consecutively 3 years following an eruption, and both sets of ENSO magnitudes showed a decreasing trend.     

Salinivibrio sp.YH4胞外丝氨酸蛋白酶EYHS耐盐性及生物信息学分析

摘要：目的 探讨菌株Salinivibrio sp.YH4分泌的丝氨酸蛋白酶EYHS的耐盐性及结构特征。方法 明胶底物酶谱法分析EYHS的耐盐性。应用生物信息学手段对EYHS及6种耐盐的S8家族丝氨酸蛋白酶结构特征进行分析。结果 EYHS在4 mol/L的NaCl溶液中仍具有活性,属于耐盐蛋白酶。EYHS及6种S8家族丝氨酸蛋白酶分子表面的loop区等无规则卷曲所占比例较高,α-螺旋与β-片层则主要位于酶分子内部。EYHS分子表面酸性氨基酸含量较高,且具有弱疏水内核。多序列比对发现蛋白酶的催化三联体两侧存在高度保守的基序和保守的极性氨基酸及芳香族氨基酸,并存在多个保守的Gly与Ala。同源模建和表面电荷分布显示,α螺旋和β片层围成了蛋白酶的催化腔,EYHS活性中心包含由Asp32、His65与Ser215组成的催化三联体,且催化位点区域表面静电势为负。结论 上述结构特征可能有助于耐盐丝氨酸蛋白酶EYHS在高盐环境下维持其稳定性和适度柔性,并有助于其催化功能的发挥,为深入研究耐盐丝氨酸蛋白酶的高盐环境适应性提供了一定的理论依据。