α-1,4-葡聚糖裂解酶的动力学性质研究

红藻中的α-1,4-葡聚糖裂解酶是红藻淀粉的降解酶,除了 红藻淀粉外,它还可以作用于多种底物。文中分别以PNPG和可溶性淀粉作底物,以龙须菜的 几个品系作为材料,研究了该酶促反应的动力学方面的性质。以 PNPG作为底物,该酶在60m in内酶促反应速度与保温时间成线性关系;在野生型龙须菜品系中该酶的最适反应温度是50 ℃,在两种突变型品系中是40℃;最适pH范围在4.4～5.5;底物浓度是4mmol/L时达到最大反 应速度;葡萄糖对该酶促反应有明显的抑制作用。以可溶性淀粉作为底物,在反应60 min内 酶反应速度与保温时间成线性关系;最适反应温度为50℃;最适pH范围在5.0～5.8;底物浓 度是8mg/mL时达到最大反应速度;葡萄糖对可溶性淀粉的抑制作用比对PNPG的弱。     

海上平台桶基负压沉贯阻力与土体稳定数值计算研究

用有限元分析方法对桶形基础负压沉贯过程中流场进行动态模拟,提出桶形基础沉贯阻力计算方法和土体稳定有限计算方法,提出桶内土体稳定性判断准则,计算结果表明所提方法是合理的,并具有工程实用性。     

海涂围垦工程中悬挂式爆破挤淤基础处理技术探讨

沿海软土地基围涂工程中悬挂式爆破挤淤基础处理断面结构设计中遇到的最大难题是对腰宽的处理,腰宽太窄则工后沉降大,反之,则断面石方量增大,投资增加,因此,试图通过工程实例说明悬挂式爆破挤淤基础处理断面设计及工程实施过程中遇到的问题及对策措施。     

南极菲尔德斯半岛土壤微生物在富集传代培养中对菲的响应

为揭示富集传代培养过程中南极多环芳烃降解菌群的演替规律,以菲为唯一碳源和能源对南极菲尔德斯半岛不同地理位置的6份土壤样品进行了富集和连续传代培养,并采用高通量测序技术分析了富集传代培养过程中菲降解菌群的群落结构及生物多样性.Alpha多样性分析结果表明,在富集培养阶段南极菲降解菌的物种丰富度显著高于传代培养阶段(P＜0...     

浮力装置触发深海管道水平向整体屈曲效果的数值模拟研究

海洋油气资源的运输主要通过海底管道进行,管道在工作时受到较大的温度荷载,会产生整体屈曲变形。深海管道设计中常采用人为装置触发一定程度的水平向整体屈曲变形,来释放轴向的温度应力,浮力装置是常用的触发方式之一。本文通过数值模拟研究,分析了不同浮力大小和不同浮力施加范围下,管道水平向整体屈曲的临界屈曲力,得出临界屈曲力随浮力大小和施加范围变化的情况;并研究了不同土体阻力下,浮力装置触发整体屈曲的效果。研究表明,水平向土体阻力较大时,浮力装置触发水平向整体屈曲的效果较好。浮力装置的触发效果对轴向土体阻力不敏感。     

长江口南汇湿地植被的光谱吸收特征研究

植被的光谱吸收特征与植被生长状况密切相关,同时也受土壤湿度等外界因素影响。本文以长江口南汇湿地典型植被芦苇、互花米草和海三棱藨草为研究对象,在计算其光谱特征参数的基础上,分析了这3种植被的光谱吸收特征形变PSDI值。最后结合实测的土壤湿度,分析了3种植被的PSDI值和土壤湿度的相关性。研究结果发现:互花米草的PSDI值最大,海三棱藨草的PSDI值最小,芦苇介于两者之间,表明互花米草受外界的环境影响较大;互花米草的PSDI值与土壤湿度的相关性最大,芦苇的PSDI值与土壤湿度的相关性最小,海三棱藨草介于两者之间,表明互花米草适应湿地环境能力较强。     

Does compaction-induced subsidence control accommodation space at the top of prograding carbonate platforms? Constraints from the numerical modelling of the Triassic Esino Limestone (Southern Alps,Italy)

The demise of the high-relief, steep-slope, prograding Ladinian-Early Carnian carbonate platforms of the Esino Limestone (Central Southern Alps of Italy) is marked by subaerial exposure of the platform top associated with different erosional (mainly karst-related), depositional and diagenetic processes (Calcare Rosso). The exposure-related deposits consist of three major facies associations: 1) residual soils with thin lenses of conglomerates with black pebbles, and, locally, weathered vulcanites; 2) chaotic breccia lenses irregularly distributed in the uppermost part of the Esino Limestone carbonate platform, interpreted as collapse breccias in karstic setting: 3) inter-supratidal carbonate cycles with dissolution and development of paleosols and tepee structures.Facies distribution follows the sub-environments of the underlying Esino Limestone. Facies 1 and 2 typically characterize the core of the platform, covering the underlying inner platform facies. Facies 3 instead develops toward the edge of the platform, above reef-upper slope facies of the prograding facies of the Esino Limestone. The thickness of facies 3 decreases toward the core of the platform. Facies distribution reflects differences in the accommodation space and sedimentary processes from the rim (highest accommodation, favouring the deposition of peritidal-supratidal carbonates) to the core (reduced accommodation, causing pedogenesis and karstification) of the carbonate system.The observed thickness changes may be controlled by different factors: 1) syndepositional tectonics, 2) subsidence induced by magmatic activity or 3) differential subsidence controlled by the stratigraphic architecture of the Esino Limestone platform and adjoining basins. As evidence of tectonics was not observed and the presence of volcanic bodies is only documented tens of km away from the study area, the scenario involving the creation of accommodation space by compaction of the basinal sediments (resedimented, fine-grained calciturbidites) during the progradation of the carbonate platform is here investigated. Numerical modelling was performed to verify the compatibility of compaction-induced subsidence with the observed depositional architecture. The models were built to simulate the architectural evolution of the platform by progressively adding layers from deepest to shallowest, while compacting the underlying sediments, in order to evaluate compaction-induced subsidence (and accommodation space for the Calcare Rosso) after the deposition of the youngest platform strata. Modelling results allow us to conclude that the wedge geometry of the Calcare Rosso, deposited on top of the extinct Esino carbonate platform, can be explained by subsidence controlled by compaction of the basinal sediments present below the early-cemented, fast prograding platform slope deposits.     

Investigation on the Effect of Geometrical and Geotechnical Parameters on Elongated Offshore Piles Using Fuzzy Inference Systems

Among numerous offshore structures used in oil extraction, jacket platforms are still the most favorable ones in shallow waters. In such structures, log piles are used to pin the substructure of the platform to the seabed. The pile’s geometrical and geotechnical properties are considered as the main parameters in designing these structures. In this study, ANSYS was used as the FE modeling software to study the geometrical and geotechnical properties of the offshore piles and their effects on supporting jacket platforms. For this purpose, the FE analysis has been done to provide the preliminary data for the fuzzy-logic post-process. The resulting data were implemented to create Fuzzy Inference System (FIS) classifications. The resultant data of the sensitivity analysis suggested that the orientation degree is the main factor in the pile’s geometrical behavior because piles which had the optimal operational degree of about 5° are more sustained. Finally, the results showed that the related fuzzified data supported the FE model and provided an insight for extended offshore pile designs.     

Deltaic soil behavior classification using CPTu records—Proposed approach and applied to fifty-four case histories

For the past four decades, the CPT has played a key role in onshore and offshore soil investigations. One of the main applications of cone penetration test (CPT) is the soil behavioral classification. Most of the developed methods for soil identification using CPT and CPTu (piezocone) data are well categorized for common soils, such as clays, silts, and sands. Soils with low resistance or more compressibility generally involve problems in geotechnical engineering practice and construction projects. Consequently, these unusual deposits require further evaluation and more detailed data. Five major groups of problematic soils including: liquefiable, sensitive, peaty, collapsible, and expansive soils have been considered in this study. One hundred and forty CPT and CPTu test records were collected from fifteen countries. Sixty-one of the records are related to difficult soils. A brief comparison is performed for currently used soil behavioral classification charts, such as by Campanella et al. (1985 Campanella, R. G., P. K. Robertson, D. Gillespie, and J. Greig. 1985. Recent developments in in-situ testing of soils. Proceedings of 11th International Conference on Soil Mechanics and Foundation Engineering, ICSMFE, San Francisco, Vol. 2, 849–54. [Google Scholar]), Robertson (1990 Robertson, P. K. 1990. Soil classification using the cone penetration test. Canadian Geotechnical Journal 27 (1):151–58. doi:10.1139/t90–014[Crossref], [Web of Science ®] , [Google Scholar]), Jefferies and Davies (1991 Jefferies, M. G., and M. P. Davies. 1991. Soil classification using the cone penetration test: Discussion. Canadian Geotechnical Journal 28 (1):173–76. doi:10.1139/t91–023[Crossref], [Web of Science ®] , [Google Scholar]) and Eslami and Fellenius (1997 Eslami, A., and B. H. Fellenius. 1997. Pile capacity by direct CPT and CPTu methods applied to 102 case histories. Canadian Geotechnical Journal 34 (6):886–904. doi:10.1139/cgj-34–6-886[Crossref], [Web of Science ®] , [Google Scholar]). Analysis based on CPT data indicates that a few commonly used charts recognize relatively well problematic deposits. However, further studies are needed to increase the accuracy and capability of methods. Existing charts have some problems due to the limitations of the nature of rectangular charts based on two axes. A new format of classification chart, i.e., triangular form containing cone tip resistance (q_c), sleeve friction (f_s), and pore pressure (u₂) is proposed for soil identification which can be realized in practice. The proposed chart with more accuracy and less scattering of data than the previous charts is able to identify soil types particularly for deltaic soils.     

Lateral dynamic response of a partially embedded pile subjected to combined loads in saturated soil

In this article, an analytical solution is proposed to investigate the lateral dynamic response of a pile which is partially embedded in saturated soil layer and subjected to combined lateral and vertical loads. The saturated soil is described by Biot’s poroelastic theory and the resistance of soil is derived by potential function method. The governing equation of the pile is solved by coupling soil resistance and continuity conditions between the pile and the soil. The dynamic impedances of the pile are then obtained through transfer matrix method. To verify the validity of the proposed procedure, the present solution is compared with available solution for an idealized case. Finally, a parametric study is performed to investigate the effects of various parameters on the stiffness and damping properties of the pile-soil system. It is found that permeability of the soil and vertical load has significant effects on the dynamic response of the pile.