海绵内生真菌胞外多糖理化性质及自由基清除活性研究

从1株海绵内生真菌(Alternaria sp.)链格孢菌的发酵液中提取胞外多糖,对其进行Q Sepharose FF离子交换色谱分离纯化,得到2个组分JJY-W和JJY-S。运用各种化学方法及波谱方法对JJY-W和JJY-S的理化性质及结构进行分析,并对多糖清除自由基活性进行了评价。结果表明,JJY-W和JJY-S的分子量分别为1.4KD和1.8KD;JJY-W以半乳糖、葡萄糖为主,含有少量甘露糖,摩尔比为:甘露糖∶葡萄糖∶半乳糖=1∶2.5∶11。JJY-S以甘露糖、葡萄糖为主,含有少量半乳糖,摩尔比为:甘露糖∶葡萄糖∶半乳糖=3∶2∶1。JJY-W总糖含量为46.3%,未检测出糖醛酸,蛋白含量为2%。JJY-S总糖含量为52%,糖醛酸含量为6.1%,蛋白含量为14%。活性分析表明,2种多糖均具有一定的体外抗氧化活性,并随着浓度的增加清除自由基能力均增强,JJY-W清除DPPH自由基的活性要强于JJY-S,而JJY-S清除OH.自由基的活性要强于JJY-W。     

磺酸型阳离子树脂的元素分配行为及高精度同位素分析应用

不同元素在离子交换树脂的分配系数是元素纯化和分离的基础,不同酸中各元素分配系数差异可用于设计高效的元素提纯流程,从而被广泛应用于现代高精度同位素分析。本文以AG®50W-X8阳离子树脂为研究对象,以分配系数(Kd)作为量化指标,通过系统实验研究不同元素在该树脂中的分配行为。在前人研究基础上,本实验增加了元素数量和酸的种类,涵盖了金属、类金属、非金属和稀土元素。结果表明：在盐酸和硝酸介质中,几乎所有元素的Kd都与酸度呈负相关,当酸度达到6mol/L时,除Th和Ca以外的所有元素都会被酸洗脱。稀土元素(REEs)和高场强元素在0.1～0.5mol/L稀硝酸和稀盐酸中强烈吸附在树脂上;而一些过渡金属、类金属和非金属元素(如Mo、W、Re、Ir、Sb、Ge、As、Se、Te等)在酸溶液中会形成含氧阴离子,不与阳离子树脂发生吸附。Al、Fe、Se、Pd、Cd、In等元素在盐酸中易与氯离子形成配位化合物或离子团,导致这些离子在盐酸中的分配系数显著降低。在硝酸和盐酸与氢氟酸的混合酸中,除稀土元素外,绝大部分元素随酸度增加Kd迅速降低。稀土元素在盐酸-氢氟酸混合介质中,随着盐酸的浓度增加(从0.1mol/L盐酸-0.2mol/L氢氟酸至6mol/L盐酸-0.2mol/L氢氟酸),稀土元素分配系数（KdREE）具有先增加后降低的趋势。氢氟酸的加入可显著降低Be、Al、Sc、Fe、Sn、Th、U、Ti、Zr、Hf等元素在稀盐酸和稀硝酸中的分配系数,使这些元素几乎不与树脂发生吸附。本研究揭示了不同酸介质中各类元素在阳离子交换树脂上的分配行为存在差异,尤其是氢氟酸的加入可显著改变高场强元素、部分过渡金属和稀土元素的分配系数,为应用该树脂开发和优化适用于高精度金属稳定同位素分析的元素提纯流程(如Li、Mg、K、Sr、Ce、U等)提供了数据支撑,并可有效地减少后续实验设计的工作量。     

鱿鱼(Squid)肝脏蛋白酶解物中ACE活性抑制肽的分离纯化及活性分析

为提高鱿鱼加工下脚料的利用率,以秘鲁鱿鱼肝脏为研究对象,采用生物酶解技术和凝胶蛋白分离技术等对其进行研究。研究结果表明:鱿鱼肝脏蛋白酶解液经过超滤处理后(分子质量为20k Da),用Sephadex G-100初步分离,得到五个组分,对分离的组分进行研究,结果表明降血压活性能力最强的是组分二,测定其半抑制浓度(IC50)为1.8mg/m L。对组分二进行活性条件分析,结构表明其ACE抑制活性在温度0—100°C、p H 1—12之间基本没有变化。最后用DEAE阴离子交换柱和Sephadex LH-20对组分二继续分离纯化,得到组分六和组分七,测定其半抑制浓度(IC50)分别达到1.52 mg/m L和2.16 mg/m L。本研究可为利用鱿鱼加工下脚料开发降血压活性肽产品提供理论基础。     

一株南极细菌（Pseudoalteromonas sp.）对模拟海水养殖水体的净化作用

试验选用一株高产低温蛋白酶的南极细菌菌株Pseudoalteromonas sp. AN64,从溶解氧、化学需氧量、氨氮、亚硝态氮、pH值5个方面研究了南极细菌对养殖水体的影响。结果显示,随着饲料中蛋白质的散失,水体中蛋白质的含量在第7天达到高峰,溶解氧下降,化学需氧量持续上升,在第11天达最大值9.88 mg/L,并且维持在较高的水平。缺氧条件下,有害物质氨氮和亚硝态氮含量逐渐增加,在第9天和第11天分别达最大值0.65 mg/L和0.352 mg/L,而pH值持续下降,在第9天达最低值5.95。当水体中加入高产蛋白酶的南极菌株AN64后,蛋白质含量在第5天即达高峰,溶解氧无明显变化,化学需氧量在第7天达最高峰,比对照组提前4天,但含量仅约为后者的一半。同时,氨氮和亚硝态氮含量远低于对照组,分别仅为对照的47.7 %和26.5 %,而pH值有小幅下降,维持在6.85到7.52之间。由此可以看出,南极菌株产生的低温蛋白酶可以有效分解海水中的蛋白质,降低水体中的氨氮和亚硝态氮等有害物质,维持pH值的稳定性,为健康养殖提供了有效途径。     

Nitrifying and Denitrifying Bacteria in Epiphytic Communities of Submerged Macrophytes in a Treated Sewage Channel

A quantification of nitrifying and denitrifying bacteria present in different compartments (water, sediments, submerged macrophytes) of a treated sewage channel was made to estimate their influence on the nitrogen balance and to assess the significance of macrophytes for nitrification and nitrogen conversions in general. Considerable numbers of autotrophic and heterotrophic nitrifying and denitrifiying bacteria were found to be present in the epiphytic communities of different species of submerged macrophytes of a treated sewage channel. Comparing the influence of the different compartments on total stream nitrification and denitrification it could be concluded that dense beds of submerged macrophytes particularly positively influence nitrification. Epiphytic nitrifiers were estimated to be as important for the total nitrification as nitrifiers in the sediment. Denitrification was mainly taking place in the sediment. The influence of the suspended nitrifiers and denitrifiers on the nitrogen balance was assumed to be negligible.     

An Ecosystem Model Coupled with Nitrogen-Silicon-Carbon Cycles Applied to Station A7 in the Northwestern Pacific

A model based on that of Kishi et al. (2001) has been extended to 15 compartments including silicon and carbon cycles. This model was applied to Station A7 off Hokkaido, Japan, in the Northwestern Pacific. The model successfully simulated the observations of: 1. a spring bloom of diatoms; 2. large seasonal variations of nitrate and silicate concentrations in the surface water; and 3. large inter-annual variations in chlorophyll-a. It also reproduced the observed features of the seasonal variations of carbon dioxide partial pressure (pCO₂)—a peak in pCO₂ in winter resulting from deep winter convection, a rapid decrease in pCO₂ as a result of the spring bloom, and an almost constant pCO₂ from summer through fall (when the effect of increasing temperature cancels the effect of biological production). A comparison of cases with and without silicate limitation shows that including silicate limitation in the model results in: 1. decreased production by diatoms during summer; and 2. a transition in the dominant phytoplankton species, from diatoms to other species that do not take up silicate. Both of these phenomena are observed at Station A7, and our results support the hypothesis that they are caused by silicate limitation of diatom growth. This revised version was published online in July 2006 with corrections to the Cover Date.     

星虫胶原蛋白的纯化及其性质的初步分析

本实验研究了水生环节动物星虫胶原蛋白的提取及纯化方法.SDS-PAGE电泳分析结果显示,所提取的胶原蛋白纯度较高.通过粘度测定分析星虫胶原蛋白的变性温度约35℃.酶解性质的研究表明,纯化的星虫胶原蛋白可被胰蛋白酶、胃蛋白酶和蛋白酶K水解.     

福建李坊重晶石矿床中硅质岩硅、氧同位素组成及成因意义

福建李坊大型重晶石矿床围岩中发育层状硅质岩,为了研究硅质岩的成因,本文对14件硅质岩样品进行了硅、氧同位素分析。结果表明:δ(³⁰Si)分布范围为-0.3×10^-3～+0.1×10^-3,平均值为-0.1×10^-3;δ(¹⁸O)分布范围为10.6×10-3～18.9×10-3,平均值为14.7×10^-3。基于该层状硅质岩的δ(³⁰Si)和δ(¹⁸O)分布范围特征,结合野外地质调查研究工作的认识,该硅质岩应为原生沉积成因,二氧化硅来源于热水沉积;根据燧石-海水氧同位素地质温度计估算石英的形成温度为100.2℃—198.7℃,平均值为146.0℃,即该硅质岩形成于热水沉积,暗示李坊重晶石矿床形成于热水沉积作用。     

放射性元素和毒性重金属污染土壤的治理探讨--以湖南雄磺矿和七一五铀矿为例

湖南雄磺矿和七一五铀矿的长期开采和冶炼,对矿山生态环境造成了严重的污染和破坏,直接影响到了矿山周边居民的正常生产与生活。从2003年起,国土资源部和国家财政部拨出了专款实施矿山地质环境治理工程。其中对放射性和毒性元素污染的土地治理都是采用的机械物理复垦土地的方法,比起国外先进的原地微生物净化处理方法来,缺少科技含量。通过介绍保加利亚中部两个矿山采用微生物净化处理污染土壤的成功范例,提出了同类矿山地质环境治理的建议。     

Mosaic and gradient SiGe single crystals for gamma ray Laue lenses

Both Ge_1−x Si _x mosaic crystals and Si_1−x Ge _x crystals with gradient of composition could be grown using the modified Czochralski technique to produce the diffracting elements for the MAX gamma ray telescope. Although many elements cut from the mosaic crystal and used before for CLAIRE gamma ray telescope had an efficiency up to 20%, the overall efficiency of the lens was about 8.1 ± 0.7 %, which is more than twice lower than the theoretically predicted efficiency. Some causes of this behaviour are discussed. In addition to mosaic crystals, the growth of Si_1−x Ge _x crystals with a gradient of composition and their properties are analysed. Such composition-gradient crystals could be a promising way to improve the diffraction efficiency of Laue lens for MAX.