Genetic variation of Laminaria japonica (Phaeophyta) populations in China as revealed by RAPD markers

For the population genetics analysis of the naturally grown brown seaweed Laminaria japonica (Laminariales,Phaeophyta) sampled from Dalian,Yantai,Weihai,Rongcheng and Qingdao in China,ten primers were employed to produce 88 bands as revealed by randomly amplified polymorphic DNA (RAPD) markers,and all these bands were polymorphic.According to these band patterns,there were 94 distinct phenotypes occurred in 100 samples indicating the high heterozygosity of this kelp.Dalian population samples showed the highest percentage of polymorphism (71.67%),and also the higher diversity estimated on the basis of the Shannon’s index (8.498),suggesting that this population could be chosen as the best resource for genetic breeding.The highest diversity of Yantai population possibly resulted from the introduction of L.longissima used for interspecific cross breeding with L.japonica cultivated in China.From Dalian southwards to Qingdao,the genetic variation of the five populations became less with a decrease in latitude,possibly due to the natural selection especially of high temperature.The genetic distance (Φ ST values) of the five populations was a little significantly correlated with the geographical distance (r=0.496) at P =0.05 by Mantel’s test.Weihai,Rongcheng and Yantai populations were closely grouped genetically together by Neighbor-joining cluster analysis probably in that the dispersal of the kelp by propagules more easily occurring in the range of relatively short distance.The analysis of molecular variance (AMOVA) also demonstrated that the relatively higher variation occurred among populations (71.49%) at an extremely significant level (P <0.000 1).All these evidence showed that there was a relatively distinct genetic differentiation among the sampled kelp populations,and L.japonica grown in China was also rather heterozygous in heredity.     

The development of spatial decision support system tool for marine spatial planning

A major problem associated with marine spatial planning (MSP) involves the difficult and time-consuming practice of creating a scenario that encompasses complex datasets in near real time via the use of a simple spatial analysis method. Moreover, decision-makers require a reliable, user-friendly system to quickly and accessibly acquire accurate spatial planning information. The development of national spatial data infrastructure (NSDI), which links the spatial data of a nation’s many diverse institutions, may pave the way for the development of a tool that can better utilize spatial datasets, such as a spatial decision support system (SDSS). Thus, this project aimed to develop an SDSS for MSP and to evaluate the feasibility of its integration within the NSDI framework. The seaweed culture was selected as an example due to its economic and technological acceptance by traditional fishers. Additionally, a multi-criteria analysis was used to develop the tool. Furthermore, a feasibility evaluation of its implementation within the NSDI framework was conducted based on the Delphi method. The results of the assessment indicated that the SDSS can be incorporated into the NSDI framework by addressing the policy issue – one map policy, updating custodians’ decree and data, and improve the standard and protocol.     

天然海藻场沿岸表层沉积物有机质的分布特征与来源

海藻场是生物多样性最高的生态系统之一, 其内部的沉积物有机质是支撑海藻场生物多样性的重要物质基础之一。本研究以浙江省嵊山岛北部无人村沿岸的天然海藻场为研究对象, 在大型海藻凋落期6~8月采集沉积物和端元生物样本, 分析了沉积物样本的粒径组成、总有机碳 (TOC)、总氮(TN)、碳氮比值(C/N)和碳氮稳定同位素(δ¹³C和δ¹⁵N), 通过贝叶斯稳定同位素混合模型评估了沉积物有机质的来源及变化规律。结果显示, 1)海藻场沿岸沉积物中粉砂占比最大, 砂的占比最低, 沉积物类型为黏土质粉砂; 2)沉积物TOC、TN、C/N、δ¹³C和δ¹⁵N范围分别为0.70%~2.41%、0.11%~0.41%、5.53~6.48、–21.79‰~–19.60‰和1.56‰~4.26‰, 在空间分布上, TOC与TN含量均随离岸距离增加而下降; 3)沉积物粒径组成、C/N比值、δ¹³C和δ¹⁵N之间的关系显示沉积物有机质主要来源于大型海藻和浮游植物的混合贡献; 4)根据贝叶斯同位素混合模型计算结果显示, 大型海藻对沉积物有机质贡献率在2.30%~45.60%, 在空间分布上, 大型海藻对沉积物有机质的贡献率随离岸距离增加而下降; 5)海藻碎屑产生量和沉积物有机碳沉积量评估结果显示, 大型海藻产生的碎屑有机质中有11.98%进入海藻场及沿岸海域的表层沉积物中。本研究为评估海藻场碎屑的产生规模和时空分布, 以及渔业资源养护功能提供了数据支撑。     

Relationship between long‐term changes in algal community structure and herbivore diet at the Three Kings Islands,New Zealand

We examined temporal changes at the Three Kings Islands, New Zealand in 1993 and 2002 in the diet of the endemic herbivorous fish Odax cyanoallix (Family Labridae) and in habitat‐forming macroalgae, and compared these with published accounts from 1979. The diet of O. cyanoallix predominantly comprised the laminarian Ecklonia radiata in 1993, and the fucoids Sargassum johnsonii and Landsburgia quercifolia in 2002. In depths <10 m, the endemic fucoid S. johnsonii was dominant in 1979 and 2002 and absent in 1993. E. radiata was absent in 1979, abundant in 1993, and rare in 2002. A period of significantly lower sea‐surface temperatures (SST) at the Three Kings Islands in 1990–95 corresponded to an unusually long El Niño event. The low SST may have resulted in the failure of S. johnsonii to grow and/or recruit during the El Niño event, leading to the observed changes. This study demonstrates variability in fucoid and laminarian assemblages over large time scales that are reflected in diet shifts in a species of herbivorous fish.     

Seasonal variation and structure of a decapod (Crustacea) assemblage living in a Caulerpa prolifera meadow in Cádiz Bay (SW Spain)

The decapod taxocoenosis living in shallow muddy bottoms with the green algae Caulerpa prolifera was studied monthly between February 1994 and January 1996 in the Inner Bay of Cádiz (SW Spain). More than 32,000 specimens belonging to 35 species were collected. Six species were dominant (representing the 85.8% of the total number of specimens), but the structure of the taxocoenosis was regulated by the Hippolyte species, Sicyonia carinata, Palaemon adspersus and Liocarcinus arcuatus. There was no significant qualitative difference between years. There was no clear change in the dominance of groups of species during the year, as happened in the outer Bay. This is probably due to the sheltered character of the area and the more stable and dense vegetal cover, but some seasonal differences were found.The benthic characteristics of the Inner Bay of Cádiz, such as shallow soft bottoms of fine and muddy sediments and the presence of macrophytes (seagrasses and seaweeds) might be key factors influencing the composition and structure of the general and seasonal decapod assemblage. In spite of human impacts on the bay (e.g. aquaculture activities, sewage), the values of the diversity, equitability and richness indexes appeared stable over time (higher than those found in outer adjacent areas) and no significant differences between years were found.     

The putative impacts of the non‐native seaweed Sargassum muticum on native communities in tidepools of Southern California and investigation into the feasibility of local eradication

The ecological impacts of introduced seaweeds have been relatively understudied. Current research suggests that seaweed invasions often result in alterations of native marine communities and disruptions of normal ecosystem functioning, but the effects on native communities can vary among invasive seaweed species, among habitats and over small and large spatial scales. In this study, the impacts of Sargassum muticum, a non‐native brown alga introduced into southern California, USA, several decades ago, were examined by comparing community structure in rocky inter‐tidal pools with and without the seaweed. Sargassum muticum appeared to have little impact on the native community despite measures revealing changes in the abiotic conditions of pools, with S. muticum presence reducing light penetration and ameliorating pool temperature changes during low tides. In other regions and habitat types, S. muticum presence often, but not consistently, resulted in declines in macrophyte diversity and/or abundance and increases in faunal assemblages. The lack of effects of S. muticum in this study, combined with variable impacts by S. muticum and other invasive seaweeds worldwide, suggests that predicting the effects of introduced seaweeds is problematic and warrants further research. Regardless of the effects on native communities, there is often a desire to eradicate or control the spread of non‐native seaweeds. In this study, localized S. muticum eradication attempts, including manipulations of a native canopy and herbivorous urchins, proved unsuccessful as full recovery occurred in ~9 months. While eradication efforts conducted worldwide have resulted in mixed success, there is a trend that early detection and rapid response can increase success, highlighting a need for systematic monitoring and establishment of regional rapid response plans.     

几种海藻功能寡糖的结构、制备、活性与应用研究进展

海洋来源的功能寡糖具有多种生物活性,如抗炎、抗氧化、抗病毒、抗肿瘤和免疫调节等,因此在医药、农业、化工及环境等领域有着广泛的应用.然而,不同聚合度、不同结构的寡糖所具有的活性差异很大,因此有必要深入研究和探讨不同种类的海洋功能寡糖的结构与功能的关系及其应用潜力.本文对几种研究较多的海藻功能寡糖的来源、制备、纯化及应用进...     

陆源排污对邻近海域底栖海藻群落的影响

伴随着经济的发展,我国沿海海域富营养化日趋严重。为了明确富营养化对底栖海藻群落的影响及作用机制,本文选择了与大连凌水河口(污水河)毗邻的海藻床作为野外观测点,研究底栖海藻群落对营养盐自然衰减梯度的响应规律;在实验室内,选择了在排污口附近优势分布、营r-生态策略的绿藻缘管浒苔和仅在寡营养区域分布、营k-生态策略的红藻柔质仙菜作为实验材料,开展了营养吸收动力学和生长动力学研究。通过二者在营养盐吸收、利用和繁殖策略方面的比较研究,剖析了底栖海藻群落对不同营养环境的响应机制。结果显示:随着海水中营养盐浓度的降低,底栖海藻群落呈现种类数增加、优势种覆盖度降低的趋势。根据底栖海藻在群落水平对氮源营养的响应,认为现行海水水质标准中无机氮一类水质标准的限值应该由目前的14.29 μmol/L降低为6.69 μmol/L。在富营养环境中,营养盐浓度的上升促进了r-策略海藻幼体的竞争力和种群繁殖力,使其占据了大量的生态位,形成优势种群,导致底栖海藻多样性较低;在寡营养环境中,由于得不到充足的营养盐供应,r-策略海藻幼体的竞争力和种群繁殖力都受到制约,占据的空间生态位有限,为其他种类的生存提供了条件,而那些对营养盐需求较低但利用效率高的k-策略海藻则表现出更强的竞争力,在竞争中取得优势,能快速突破早期环境筛的限制,形成成体,因此,在寡营养海域,底栖海藻的多样性比较丰富。     

中国海洋生物研究70年

随着中国“海洋强国”战略的提出,加快建设海洋类学科的发展成为历史必然,海洋生物是海洋不可分割的一部分,海洋环境和生物相互依存,相互作用,海洋生物研究重要性日益凸显。为纪念中国科学家在海洋生物领域的突出贡献,本文回顾了建国以来中国海洋生物相关的重要研究进展,梳理了中国科学家在海洋生物领域的突出贡献,系统总结并讨论了未来研究方向,抛砖引玉,希望籍此助推中国海洋生物研究的新高潮。     

硇洲马尾藻Sargassum naozhouense幼孢子体附着及原生态恢复

【目的】探究硇洲马尾藻(Sargassum naozhouense)幼孢子体附着的选择性及原生态位点固着人工藻礁对马尾藻海藻资源恢复效果。【方法】选用混凝土、黄泥砖、牡蛎壳、聚丙烯板、橡皮条、沥青等6种废弃物材质对硇洲马尾藻幼孢子体附着效果进行比较;将硇洲马尾藻于徐闻南山进行原生态位点投放,周年观察比较人工藻礁与自然藻礁生长差异,探究硇洲马尾藻资源恢复效果。【结果】(1)不同材质藻礁对硇洲马尾藻幼孢子体附着差异显著,附着效果依次为混凝土>黄泥砖>牡蛎壳>聚丙烯板>橡皮条,沥青完全没有幼孢子附着。(2)当年人工藻礁苗有性生殖苗较自然生态苗生长慢,第二年假根再生苗生长与自然苗生长无显著性差异。(3)人工藻礁苗成活率为29.2%,此后不出现消退死亡,并以假根再生维持种群的繁衍。【结论】混凝土是马尾藻原生态藻床恢复较理想的人工附着基,黄泥砖、牡蛎壳、聚丙烯板等废弃物也可用于天然硇洲马尾藻以及室内有性苗源的附着基。假根再生是马尾藻种群繁衍的主要方式,利用有性繁殖的幼孢子体附着于混凝土块上进行硇洲马尾藻原生态位点投放是短时间增加马尾藻资源的有效措施,可为硇洲马尾藻海藻资源恢复提供可行的方法。