应用巢式逆转录聚合酶链反应检测鱼类病毒性出血性败血症病毒(VHSV)

参照鱼类病毒性出血性败血症病毒序列,根据PCR引物设计的原则,设计巢式PCR引物,采用巢式逆转录聚合酶链反应(RT-PCR)方法,对如何快速检测鱼类病毒性出血性败血症病的方法进行了较为系统的研究,并对RT-PCR的反应条件进行了优化.结果表明,巢式RT-PCR扩增获得279bp的特异性片断,阴性对照无扩增条带.巢式RT-PCR扩增出的特异性片段经测序分析,结果证实与报道的序列完全一致.该方法最低可检测出0.1pg的鱼类病毒性出血性败血症病毒RNA.初步建立了VHSV的巢式RT-PCR检测方法,该方法灵敏、特异,可为VHSV的检测提供一个快速、有效的手段.     

低聚壳聚糖的美拉德反应及其衍生物的抗氧化性能研究

醇沉法制得低聚壳聚糖美拉德反应8、16 h以及24 h的衍生物,分别记为CC-8、CC-16以及CC-24。对3种衍生物进行红外表征和分子量测定,并研究其对超氧阳离子O 2&、DPPH的清除能力以及还原能力。结果显示:反应体系pH呈下降趋势;UV-Vis光谱在280 nm波长处吸收峰有明显增强,反应16 h后增长缓慢;在343 nm的激发波长和420 nm发射波长下的荧光强度明显增高,反应16 h后开始下降;3种衍生物均保留着低聚壳聚糖的特征吸收峰;其对O 2&、.DPPH的清除能力以及还原能力均得到显著提高,且CC-16抗氧化能力最好。     

礁坪上波浪传播的数值模拟

本文基于具备间断捕捉能力的二阶全非线性Boussinesq数值模型,对规则波和随机波在礁坪地形上的传播变形进行了数值模拟。该模型采用高阶有限体积法和有限差分方法求解守恒格式的控制方程,将波浪破碎视为间断,同时采用静态重构技术处理了海岸动边界问题。重点针对礁坪上波浪传播过程中的波高空间分布和沿程衰减,礁坪上的平均水位变化,以及波浪能量频谱的移动和空间差异等典型水动力现象开展数值计算。将数值结果与实验结果对比,两者吻合情况良好,验证了模型具有良好的稳定性,具备模拟破碎波浪和海-岸动边界的能力,能较为准确地模拟波浪在礁坪地形上的传播过程中发生的各种水动力现象。     

中地壳的地球化学动力学和矿石成因

笔者重点进行了大于300℃——在近临界区至超临界区条件下的硅酸盐矿物与水反应动力学实验。矿物(钠长石Ab、透辉石Di、阳起石Act和磁铁矿Mt)的溶解反应动力学实验是使用流体通过叠层反应器的开放体系在25～400℃和22MPa下完成的。实验发现矿物在300℃至400℃范围,在跨越水临界点时出现反应速率的涨落。各种多金属氧化物硅酸盐与水反应时,各个元素溶解到溶液里的释放速率一般不一样,常称为一致溶解作用。但是,在近300℃变为一致溶解作用。实验发现在22MPa时硅酸盐矿物的最大溶解反应速率多是在300℃,如硅的最大释放速率是在300℃。其余元素如Na、K、Mg、Ca、Fe、Al等释放速率在<300℃22MPa时都高于硅的释放速率,在>300℃时硅的释放速率要高于其它元素的释放速率。确切地说,金属与氧之间的键的性质决定了它们(金属氧化物)与水之间反应速率。在一般情况下,Na-Obr,Ca-Obr,Mg-Obr,Al-Obr和Si-Obr的键桥(br),它们之间相对地由具有离子键性质逐步变为具有极性键的性质。由常温常压到亚临界区(300～374℃22MPa),再到大于临界点374℃、22MPa进入超临界区,水的性质随温度、压力变化。水由容易溶解离子键逐渐变为容易打破极性键。笔者还研究了黑钨矿、锡石(玄武岩、花岗闪长岩)与水在250～400℃条件下的反应动力学过程,得出了相同的结果。实验均发现在跨越水临界点时矿物(或岩石)与水反应的动力学涨落。这些实验结果可以用于说明中地壳上部的水/岩相互作用的特征。发生于中地壳的水、岩相互作用大多是在300～450℃和20～50MPa条件下进行的。各地区的地壳厚度不一,中地壳温度压力并不完全相同。模拟中地壳条件下水/岩相互作用实验,目的主要是研究矿物(或岩石)在300～450℃条件下反应动力学过程。已有热液矿床矿物流体包体数据表明:有一批矿床的主要矿石形成于300～500℃,低于NaCl H2O溶液临界线的条件。中地壳的流体处于由亚临界态跨越临界态,进入超临界流体太的演化过程。这种流体的性质变化会引起水/岩相互作用的反应动力学涨落和矿石大量沉淀。     

泉州湾赤潮藻类优势种细胞密度回归方程研究

于2006年5-11月对东海泉州湾赤潮监控区四个监测站位开展赤潮常规监测。根据监测结果,分别以各站位23项水质理化生物环境因子指标为自变量,相应赤潮藻类优势种的细胞密度为因变量,进行多元逐步回归分析,建立了各站位优势种中肋骨条藻(Skeletonema costatum)、太平洋海链藻(Thalassiosira pacifica)、微小原甲藻(Prorocentrum minimum)、尖刺拟菱形藻(Pseudo-nitzschia pungens)、丹麦细柱藻(Leptocylindrus danicus)和旋链角毛藻(Chaetoceros curvisetus)等的细胞密度多参量回归方程。结果表明,所有回归方程的复相关系数都接近于1,方差分析的结果均为回归极显著,表明所建立的回归方程可作为相应赤潮优势种细胞密度预报方程的高度有效性,将对今后泉州湾的赤潮预报提供良好的指导作用。     

New finite-gap solutions for the coupled Burgers equations engendered by the Neumann systems

On the tangent bundle TS^N-1 of the unit sphere S^N-1, this paper reduces the coupled Burgers equations to two Neumann systems by using the nonlinearization of the Lax pair, whose Liouville integrability is displayed in the scheme of the r-matrix technique. Based on the Lax matrix of the Neumann systems, the Abel--Jacobi coordinates are appropriately chosen to straighten out the restricted Neumann flows on the complex torus, from which the new finite-gap solutions expressed by Riemann theta functions for the coupled Burgers equations are given in view of the Jacobi inversion.     

A Time-Domain Coupled Model for Nonlinear Wave Forces on A Fixed Box-Shaped Ship in A Harbor

A 2-D time-domain numerical coupled model is developed to obtain an efficient method for nonlinear wave forces on a fixed box-shaped ship in a harbor.The domain is divided into an inner domain and an outer domain.The inner domain is the area beneath the ship and the flow is described by the simplified Euler equations.The other area is the outer domain and the flow is defined by the higher-order Boussinesq equations in order to consider the nonlinearity of the wave motions.Along the interface boundaries between the inner domain and the outer domain,the volume flux is assumed to be continuous and the wave pressures are equal.Relevant physical experiment is conducted to validate the present model.It is shown that the numerical results agree with the experimental data.Compared with the coupled model with the flow in the inner domain governed by the Laplace equation,the present coupled model is more efficient and its solution procedure is more simple,which is particularly useful for the study on the effect of the nonlinear wave forces on a fixed box-shaped ship in a large harbor.     

Velocity potential formulations of highly accurate Boussinesq-type models

The highly accurate Boussinesq-type equations of Madsen et al. (Madsen, P.A., Bingham, H.B., Schäffer, H.A., 2003. Boussinesq-type formulations for fully nonlinear and extremely dispersive water waves: Derivation and analysis. Proc. R. Soc. Lond. A 459, 1075–1104; Madsen, P.A., Fuhrman, D.R., Wang, B., 2006. A Boussinesq-type method for fully nonlinear waves interacting with a rapidly varying bathymetry. Coast. Eng. 53, 487–504); Jamois et al. (Jamois, E., Fuhrman, D.R., Bingham, H.B., Molin, B., 2006. Wave-structure interactions and nonlinear wave processes on the weather side of reflective structures. Coast. Eng. 53, 929–945) are re-derived in a more general framework which establishes the correct relationship between the model in a velocity formulation and a velocity potential formulation. Although most work with this model has used the velocity formulation, the potential formulation is of interest because it reduces the computational effort by approximately a factor of two and facilitates a coupling to other potential flow solvers. A new shoaling enhancement operator is introduced to derive new models (in both formulations) with a velocity profile which is always consistent with the kinematic bottom boundary condition. The true behaviour of the velocity potential formulation with respect to linear shoaling is given for the first time, correcting errors made by Jamois et al. (Jamois, E., Fuhrman, D.R., Bingham, H.B., Molin, B., 2006. Wave-structure interactions and nonlinear wave processes on the weather side of reflective structures. Coast. Eng. 53, 929–945). An exact infinite series solution for the potential is obtained via a Taylor expansion about an arbitrary vertical position z = zˆ. For practical implementation however, the solution is expanded based on a slow variation of zˆ and terms are retained to first-order. With shoaling enhancement, the new models obtain a comparable accuracy in linear shoaling to the original velocity formulation. General consistency relations are also derived which are convenient for verifying that the differential operators satisfy a potential flow and/or conserve mass up to the order of truncation of the model. The performance of the new formulation is validated using computations of linear and nonlinear shoaling problems. The behaviour on a rapidly varying bathymetry is also checked using linear wave reflection from a shelf and Bragg scattering from an undulating bottom. Although the new models perform equally well for Bragg scattering they fail earlier than the existing model for reflection/transmission problems in very deep water.     

A temporally second-order accurate Godunov-type scheme for solving the extended Boussinesq equations

A numerical scheme for solving the class of extended Boussinesq equations is presented. Unlike previous schemes, where the governing equations are integrated through time using a fourth-order method, a second-order Godunov-type scheme is used thus saving storage and computational resources. The spatial derivatives are discretised using a combination of finite-volume and finite-difference methods. A fourth-order MUSCL reconstruction technique is used to compute the values at the cell interfaces for use in the local Riemann problems, whilst the bed source and dispersion terms are discretised using centred finite-differences of up to fourth-order accuracy. Numerical results show that the class of extended Boussinesq equations can be accurately solved without the need for a fourth-order time discretisation, thus improving the computational speed of Boussinesq-type numerical models. The numerical scheme has been applied to model a number of standard test cases for the extended Boussinesq equations and comparisons made to physical wave flume experiments.