基于核酸适配体功能化石墨纳米颗粒荧光探针的17β-雌二醇快速检测方法

为构建一种能够简单、快速、特异性检测复杂环境水体中雌激素污染的方法,利用石墨纳米颗粒作为荧光淬灭剂、核酸适配体作为识别元素、1-芘丁酸N-羟基琥珀酰亚胺酯作为异型双功能交联剂,构建了一种新型纳米荧光探针;并探究了构建荧光探针时核酸适配体初始投加量和荧光探针投加量对雌二醇检测的影响及最佳实验条件下检测雌二醇的效果和特异性。实验结果表明：核酸适配体能成功修饰在石墨纳米颗粒表面形成的稳定荧光探针;构建荧光探针时核酸适配体的最佳初始投加量为1.0 μmol/L;检测雌二醇时,荧光探针的最佳投加量为4 μg/mL;最佳实验条件下,相对荧光强度与雌二醇的质量浓度在50~800 ng/mL范围内成正比,最低检测限为34.5 ng/mL,且该荧光探针能实现对雌二醇的简单、快速、特异性检测。     

浒苔光照和盐度胁迫响应基因UpMYB44的克隆与表达分析

为证明R2R3-MYB转录因子在浒苔响应非生物胁迫例如盐度和光照的过程中发挥的重要调控作用,利用实时荧光定量PCR、酵母双杂交系统、亚细胞定位等技术,研究获得了浒苔UpMYB44基因1 437 bp的开放阅读框(ORF)全长序列,编码478个氨基酸,属于典型的R2R3-MYB转录因子并通过烟草叶片转化确定其定位于细胞核,UpMYB44参与浒苔响应光照和盐度的胁迫过程,其中在低光和高盐胁迫下UpMYB44基因的相对表达量升高。筛选出了与UpMYB44互作的UpCPP5蛋白,该蛋白与浒苔的生长和细胞分裂有关,推测UpMYB44可能通过与UpCPP5互作,形成蛋白复合体参与浒苔的增殖过程。研究为日后深入探究MYB类转录因子家族调控藻类生长发育的过程奠定了基础,同时为研究浒苔快速繁殖的机制提供了思路。     

Exploring future changes in smallholder farming systems by linking socio-economic scenarios with regional and household models

We explore how smallholder agricultural systems in the Kenyan highlands might intensify and/or diversify in the future under a range of socio-economic scenarios. Data from approximately 3000 households were analyzed and farming systems characterized. Plausible socio-economic scenarios of how Kenya might evolve, and their potential impacts on the agricultural sector, were developed with a range of stakeholders. We study how different types of farming systems might increase or diminish in importance under different scenarios using a land-use model sensitive to prices, opportunity cost of land and labour, and other variables. We then use a household model to determine the types of enterprises in which different types of households might engage under different socio-economic conditions. Trajectories of intensification, diversification, and stagnation for different farming systems are identified. Diversification with cash crops is found to be a key intensification strategy as farm size decreases and labour costs increase. Dairy expansion, while important for some trajectories, is mostly viable when land available is not a constraint, mainly due to the need for planting fodders at the expense of cropland areas. We discuss the results in relation to induced innovation theories of intensification. We outline how the methodology employed could be used for integrating global and regional change assessments with local-level studies on farming options, adaptation to global change, and upscaling of social, environmental and economic impacts of agricultural development investments and interventions.     

微动线型台阵数据快速处理研究

针对城市微动探测快速实施的需求,开发了一套可视化微动探测数据快速处理软件。该软件主要功能包括微动野外观测报表校核、数据格式转换、微动有效授时数据段挑选、台阵数据截取、台阵参数计算、频散曲线提取及测线显示等,可较大地提高微动线型台阵观测及数据处理速度。沿着本文思路,可同样进行三角形台阵滚动观测数据的快速处理。     

一种加快BDS精密单点定位初始化的方法

由于BDS卫星的星座特性及卫星的轨道和钟差的精度影响,使得传统消电离层组合精密单点定位(PPP)的初始化时间较长。针对上述问题,文中对附加电离层约束的非组合精密单点定位算法进行研究。首先介绍非组合PPP算法,分析其与传统PPP的差异;其次分别利用CODE电离层格网产品,以反距离加权算法计算的站星电离层延迟、低阶球谐函数建立的区域电离层产品等作为先验信息对非组合PPP进行约束。通过MGEX观测网实测数据静态和仿动态计算表明,相比传统消电离层组合PPP,附加电离层约束的非组合PPP能够有效缩短初始化时间,同时能够获得高精度的定位结果。     

NUMERICAL SIMULATION STUDY ON THE RAPID INTENSIFICATION OF TYPHOON HAIKUI (1211) OFF THE SHORE OF CHINA

Forecasting the rapid intensification of tropical cyclones over offshore areas remains difficult. In this article, the Weather Research and Forecast (WRF) model was used to study the rapid intensification of Typhoon Haikui (1211) off the shore of China. After successful simulation of the intensity change and track of the typhoon, the model output was further analyzed to determine the mechanism of the rapid change in intensity. The results indicated that a remarkable increase in low-level moisture transportation toward the inner core, favorable large-scale background field with low-level convergence, and high-level divergence played key roles in the rapid intensification of Typhoon Haikui in which high-level divergence could be used as an indicator for the rapid intensity change of Typhoon Haikui approximately 6 h in advance. An analysis of the typhoon structure revealed that Typhoon Haikui was structurally symmetric during the rapid intensification and the range of the eyewall was small in the low level but extended outward in the high level. In addition, the vertically ascending motion, the radial and tangential along wind speeds increased with increasing typhoon intensity, especially during the process of rapid intensification. Furthermore, the intensity of the warm core of the typhoon increased during the intensification process with the warm core extending outward and toward the lower layer. All of the above structural changes contributed to the maintenance and development of typhoon intensity.     

A STUDY OF RAPID DECAY TYPHOONS IN OFFSHORE WATERS OF CHINA

On the basis of NCEP/NCAR reanalysis data and yearbooks of CMA tropical cyclones, statistical analysis is performed for 1949—2013 offshore typhoons subjected to rapid decay(RD). This analysis indicates that RD typhoons are small-probability events, making up about 2.2% of the total offshore typhoons during this period. The RD events experience a decadal variation, mostly in the 1960 s and 1970 s(maximal in the 1970 s), rapidly decrease in the 1980 s and 1990 s and quickly increase from 2000. Also, RD typhoons show remarkable seasonal differences: they arise mainly in April and July-December, with the prime stage being in October-November. The offshore RD typhoons occur mostly in the South China Sea(SCS) and to a lesser extent in the East China Sea(ECS); however, none are observed over the Huang Sea and Bo Sea.Composite analysis and dynamic diagnosis of the RD typhoon-related large-scale circulations are performed.Physical quantities of the composite analysis consist of 500-h Pa height and temperature fields, vapor transfer, vertical wind shear(VWS), density of core convection(DCC), and high-level jet and upper-air outflow of the typhoon. The results suggest that(1) at the 500-h Pa height field, the typhoon is ahead of a westerly trough and under the effects of its passing trough;(2) at the temperature field, the typhoon is ahead of a temperature trough, with an invading cold tongue present;(3) at the vapor transfer field, water transfer into the RD typhoon is cut off; and(4) at higher levels, the related jet weakens and the outbreak of convection becomes attenuated in the typhoon core. In addition, VWS bears a relation to the RD typhoon; in particular, strong VWS favors RD occurrence.The differences in RD events between the SCS and ECS show that for the RD, the VWS of the ECS environmental winds is markedly stronger in comparison with its SCS counterpart. The cold advection invading into the typhoons is more intense in the SCS than in the ECS, and the low-level vapor transfer and high-level outflow are weaker in the SCS RD typhoons.Data analysis shows that sea surface temperature(SST), VWS, and DCC can be employed as efficient factors to predict RD occurrence. With appropriate SST, VWS, and DCC, a warning of RD occurrence can be given 36, 30-36,and 30 h, respectively, in advance. These values suggest that atmospheric SST responses lag. Owing to this time lag,the prediction of RD typhoons is possible.     

地震灾害死亡人口快速评估方法对比研究

中国是世界上地震活动最为频繁的国家之一,属于地震多发区。在收集分析国内外关于地震灾害死亡人口评估模型的基础上,选取10种模型以2000年以来中国典型地震灾害为案例验证其适用性。结果表明：① 对于5.7级（含）以下地震,10种模型的评估结果基本都在合理范围内,可采用各评估结果的区间值来支撑应急决策;5.7~6.6级（含）地震,10种评估方法得到的结果表现出不同程度的偏差,可采用多数评估结果指向的死亡人口数量级来支撑应急决策;6.6级以上地震,则只能采用有特定适用范围的基于结构易损性的评估结果来支撑应急决策,且也存在较大的不确定性。② 从地震灾害人员伤亡动态评估方法、地震—地质灾害死亡人口快速评估方法和快速评估软件系统方面进行了相关讨论。此研究可为地震灾害死亡人口快速评估模型的改进与发展提供借鉴。     

Cultivated land change in the Belt and Road Initiative region

The Belt and Road Initiative (BRI)–a development strategy proposed by China – provides unprecedented opportunities for multi-dimensional communication and cooperation across Asia, Africa and Europe. In this study, we analyse the spatio-temporal changes in cultivated land in the BRI countries (64 in total) to better understand the land use status of China along with its periphery for targeting specific collaboration. We apply FAO statistics and GlobeLand30 (the world’s finest land cover data at a 30-m resolution), and develop three indicator groups (namely quantity, conversion, and utilization degree) for the analysis. The results show that cultivated land area in the BRI region increased 3.73×10⁴ km² between 2000 and 2010. The increased cultivated land was mainly found in Central and Eastern Europe and Southeast Asia, while the decreased cultivated land was mostly concentrated in China. Russia ranks first with an increase of 1.59×10⁴ km² cultivated land area, followed by Hungary (0.66×10⁴ km²) and India (0.57×104 km2). China decreased 1.95×10⁴ km² cultivated land area, followed by Bangladesh (–0.22×10⁴ km²) and Thailand (–0.22×10⁴ km²). Cultivated land was mainly transferred to/from forest, grassland, artificial surfaces and bare land, and transfer types in different regions have different characteristics: while large amount of cultivated land in China was converted to artificial surfaces, considerable forest was converted to cultivated land in Southeast Asia. The increase of multi-cropping index dominated the region except the Central and Eastern Europe, while the increase of fragmentation index was prevailing in the region except for a few South Asian countries. Our results indicate that the negative consequence of cultivated land loss in China might be underestimated by the domestic- focused studies, as none of its close neighbours experienced such obvious cultivated land losses. Nevertheless, the increased cultivated land area in Southeast Asia and the extensive cultivated land use in Ukraine and Russia imply that the regional food production would be greatly improved if China’ “Go Out policy” would help those countries to intensify their cultivated land use.     

一次局地大暴雨的地面观测资料同化预报试验研究

基于WRF（Weather Research and Forecasting）模式及其3DVAR（3-Dimentional Variational）资料同化系统,采用36 km、12 km 、4 km三层嵌套网格进行逐3 h资料同化和快速更新循环预报,对2011年5月8日鲁中一次局地大暴雨过程进行了资料同化敏感性试验。试验结果表明,地面观测资料同化和快速更新循环对本次降水的预报起到了关键性作用。在快速更新循环预报时不同化地面观测资料,或同化全部观测资料进行冷启动预报,模式均不能预报出山东的降水。同化地面观测资料后,显著改进了模式降水落区预报。地面观测资料同化可以影响到700 hPa高度以上温压湿风要素的变化,从而改变了大气初始场的温湿结构,导致模式预报的700 hPa附近高空大气湿度和热力不稳定增强,700 hPa以下低层风场更强,850 hPa鲁中以南风速较无观测资料同化的偏强2～4 m·s-1,低层风场的动力作用触发高空的不稳定大气,降水出现在山东。