Profiling Planctomycetales diversity with reference to anammox‐related bacteria in a South China Sea,deep‐sea sediment

A systematic assessment of Planctomycetales diversity in a South China Sea, deep‐sea sediment (1657 m) was conducted using the 16S rRNA gene analysis approach. PCR amplification of the samples from seven sediment layers (0.1, 1, 3, 5, 7, 9 and 11 m below the surface sediment) using the primer set Pla‐46‐F/1392‐R showed that the Planctomycetales existed within a limited range of sediment depths (≤ 5 m), and had a decreasing trend in diversity with increasing depth. The majority of the retrieved Pla‐46‐F/1392‐R sequences belonged to Pirellula‐related Planctomycetales, and two sequences retrieved from the 0.1‐m layer (GenBank accession numbers: DQ996944 and DQ996945 ) shared the same anammox‐related signature oligonucleotides and were closely related to commonly recognized anammox organisms. To identify new anammox‐related biomarkers, three primer sets were designed for amplifying the fragments of hydroxylamine oxidoreductase and S‐adenosylmethionine radical enzyme genes, but no related sequences were found. Our multiple 16S rRNA gene primer sets (Journal of Rapid Methods and Automation in Microbiology, 2008, in press) revealed even an higher diversity of Planctomycetales in the 0.1‐m layer of the sediment, especially at genus level. Our data profiled the distribution pattern of Planctomycetales diversity along sediment depths, and provided molecular evidence for the existence of anammox‐related bacteria in a new location, which broadens our understanding of Planctomycetales diversity in deep sea sediments.     

涠洲岛潮滩表层沉积物中重金属的空间分布及其环境质量评价

根据2006年4月对涠洲岛潮滩表层沉积物的调查资料,分析了表层沉积物中重金属元素Hg、Cu、Pb、Zn、Cd、Cr、As的含量水平及空间分布特征,运用单因子评价法对其环境质量进行了评估,并通过相关性分析、因子分析探讨了几种重金属元素的物源.结果表明:涠洲岛潮滩环境质量状况良好,各元素均未达到污染水平,多数地区存在Cu的玷污,个别地区存在As的玷污;各分析元素较多的累积在人为活动相对频繁的XKZ、W05断面附近区域;Hg、Cu、Zn、Cr显著正相关,以环境背景来源为主,Pb、As较多的受到人为因素的影响.     

The statistical distribution of nearbed wave orbital velocity in intermediate coastal water depth

The statistical distribution of wave orbital velocity in intermediate coastal water depth has been quantitatively determined from the comprehensive field velocity data collected near the seabed in this study. Two ocean ADV current meters, which were mounted at 0.5 m above the seabed on two separate stainless steel tripods sitting on the seabed, were used to measure instantaneous water particle velocities at a 2 Hz sampling rate for 17.07 min every hour in two coastal water depths of 11 m and 23 m in nine field deployments over a period of 2 years. The zero-crossing method is applied to analyse the field velocity data collected in each field deployment to obtain a large sample of wave orbital velocity amplitudes of individual waves. Based on the collected field velocity data, it is found that the histogram of instantaneous wave orbital velocities perfectly follows the Gaussian distribution as commonly assumed, while the histogram of wave orbital velocity amplitudes is less accurately described by the Rayleigh distribution than the modified Rayleigh and the Weibull distribution. It is also found that large orbital velocity amplitudes are generally overestimated by the Rayleigh distribution, but well predicted by the modified Rayleigh and the Weibull distribution. The expected value of maximum orbital velocity in a velocity record of finite size is also derived from the three distributions and found to agree well with the present field data.     

茂名地区海雾含水量的演变特征及其与大气水平能见度的关系

2008年3月16—19日,在广东茂名博贺海洋气象科学试验基地,利用美国DMT公司生产的FM-100型雾滴谱仪,连续观测了海雾的雾滴谱、数浓度等微物理量。在此基础上分析了海雾液态含水量的演变特征,并结合同期的气象观测与分析数据,进行了海雾中低能见度成因的天气学分析。研究表明,海雾发展过程中含水量有着上下起伏变化的特点;直径10μm以上雾滴的增多是含水量增大的主要原因;随着含水量的增大,雾滴谱分布有向大雾滴方向增宽的趋势,其峰值高度也不断增大。在相同的含水量区间,不同的大气能见度样本表现出明显不同的谱分布特征;在相同高数浓度区间的情况下,导致大气能见度降低的主要原因是含水量的增大。而海雾过程中低能见度的出现,是众多天气因素共同影响的结果:地面天气图上的均压场范围更大,大气低空及地面风速小,大气低层处于弱不稳定状态,以及高湿度层主要在近地层等等。     

Distribution patterns of interstitial polychaetes in sandy beaches of southern Brazil

This study describes the distribution patterns of interstitial polychaetes along morphodynamic gradients on six exposed sandy beaches in Santa Catarina and Paraná (South Brazil). Three random transects were sampled at two points on each beach, one at the swash and another at the surf zone, in winter and summer conditions. Six sediment replicates were collected at each sampling point using a corer of 4.6 cm internal diameter that removed 10 cm into the sediment. Abundance and composition of interstitial polychaete were correlated to wave height, slope, grain size, CaCO₃, chlorophyll a , omega indexes, temperature and relative tide range using a canonical correspondence analysis (CCA). A factorial ANOVA showed that taxa richness, mean density and Shannon's diversity were significantly higher at the reflective beaches, but average values differ significantly between transects and these differences change according to the beach zones on both sampling dates. PERMANOVA showed that polychaete associations differ among transects according to the beach zones. The composition of interstitial polychaete associations was significantly correlated to beach morphodynamics and features (P < 0.01). Polychaete associations of reflective beaches were more diverse than in other morphodynamic states. Intermediate beaches may also sustain diverse associations due to temporal variability of the morphodynamic patterns. Beaches presenting extreme dissipative morphodynamics and compacted sediments appear to be unfavourable for the occurrence of interstitial polychaetes.     

Distribution and spatial variation of hydrothermal faunal assemblages at Lucky Strike (Mid-Atlantic Ridge) revealed by high-resolution video image analysis

Whilst the fauna inhabiting hydrothermal vent structures in the Atlantic Ocean is reasonably well known, less is understood about the spatial distributions of the fauna in relation to abiotic and biotic factors. In this study, a major active hydrothermal edifice (Eiffel Tower, at 1690 m depth) on the Lucky Strike vent field (Mid-Atlantic Ridge (MAR)) was investigated. Video transects were carried out by ROV Victor 6000 and complete image coverage was acquired. Four distinct assemblages, ranging from dense larger-sized Bathymodiolus mussel beds to smaller-sized mussel clumps and alvinocaridid shrimps, and two types of substrata were defined based on high definition photographs and video imagery. To evaluate spatial variation, faunal distribution was mapped in three dimensions. A high degree of patchiness characterizes this 11 m high sulfide structure. The differences observed in assemblage and substratum distribution were related to habitat characteristics (fluid exits, depth and structure orientation). Gradients in community structure were observed, which coincided with an increasing distance from the fluid exits. A biological zonation model for the Eiffel Tower edifice was created in which faunal composition and distribution can be visually explained by the presence/absence of fluid exits.     

江苏近岸海域水文气象要素的时空分布特征

2006～2007年在江苏近岸海域进行水文气象要素(气温、湿度、风速风向、波浪、透明度、水色和海水温盐度等)大面观测。数据分析表明,各水文气象要素具有较大的空间分异,四季变化显著。秋季气温明显高于春季,冬季气温较历史数据偏高。整个海域风速较大,风向受季风控制,有效波高与风速之间显著相关。如东附近海域波浪较大,废黄河口波浪较小;春、夏季有效波高较小,而夏、秋季近岸海域波高较小,离岸波高增大。夏季海水透明度最大,平均为2.3m;冬季透明度最小,平均只有0.6m;水色与透明度呈负指数相关。春、秋季水温分布与气温分布基本一致,冬季大致沿等深线分布。射阳河口与长江口为低盐区,辐射沙脊群外缘为高盐区。与20世纪80年代的调查结果相比,出现了一些新特点。这些新的调查成果可为江苏近岸海域的海洋环境保护和可持续发展提供科学依据。     

深圳海域弧菌种类组成、数量分布及其与环境因子的关系研究

研究了深圳海域2008年4月、9月的水体弧菌数量、种类组成及其分布,探讨了弧菌总数及各优势类群的数量分布与环境因子间的相关关系。结果表明:弧菌总数分布具有季节性,东部海域春季(4月)的弧菌数量高于秋季(9月)的,其均值分别为1.50×104,8.89×102CFU/cm3;在西部海域秋季的略高于春季的,其均值分别为5.09×102,2.66×102CFU/cm3;弧菌数量最高值出现在大亚湾(4.40×104CFU/cm3)。弧菌的优势类群具有季节性分布特征:春季特有的优势类群有Vibrio gigantis类似种和V.splendidus类似种,秋季特有的优势类群有V.natriegens类似种;V.alginolyticus类似种是两个季节共有的优势类群,其数量春季高于秋季的。弧菌种类、数量与海水温度、盐度及细菌总数、Chl-a等环境因子有一定的相关性,其中盐度为弧菌种类及数量分布的主要影响因子。     

CC区多金属结核分布特征及其与地质地理环境因素之间的关系

根据１９８７、１９８８、１９９０年东北赤道太平洋ＣＣ区的３个航次多金属结核调查资料的统计分析，结果表明，ＣＣ区自西向东，从北向南，多金属结核丰度，覆盖率、形态、粒径和化学组分等具明显的区域分布规律，但在这一区域性分布背景上，结核的局部分布很复杂。