砂粒粒径与砂层厚度对单体筐养养殖系统中三疣梭子蟹幼蟹摄食行为与生长特性的影响研究*

提要 在梭子蟹的单体筐养养殖系统中,试验了生态掩体(砂盒)中不同的砂粒粒径大小以及砂层厚度对梭子蟹幼蟹摄食行为与生长特性的影响。砂粒粒径设三个水平,分别为：>2 mm、<0.2 mm以及混合砂;砂层厚度有0cm、2cm、5cm、8cm四个水平。试验共进行6天,结果表明：砂粒粒径及砂层厚度对梭子蟹幼蟹的摄食与生长都有明显的影响。从砂粒粒径看,幼蟹在细砂(SPS)中挖洞休息,蟹体与砂面呈30-45°角,仅露眼睛及触角在外。一天内有3-6次进食,总进食时间为142.7±22.52 min,在摄食次数、总进食时间、平均摄食量(0.2427±0.0137 g/gBW)、以及脱皮及成活率都远高于其他粒径组,该组中幼蟹的体重增长最快,增加了0.814±0.113 g,增长率为91.5±3.43%;而该组的饵料系数(FCR)最低为1.17±0.11。因此,筐养养殖系统砂掩体的砂粒粒径最好为0.2 mm以下。砂层厚度也有类似结果,5 cm以上厚度养殖效果最佳。平均摄食量为0.2087±0.0046 g/gBW,该厚度下,幼蟹无死亡、100%脱皮,体重也增加最快,增加了0.791±0.121 g,增长率为88.9±3.74%,饵料系数(FCR)达到1.37±0.23,表明筐养系统掩体中砂层厚度要在5 cm以上。三种保护性酶类(SOD, POD, CAT)活性随着砂粒粒径变小和砂层厚度增加而降低,而消化酶类(淀粉酶、蛋白酶、脂酶)活性则表现与保护性酶类相反特性。从两类酶的活性变化也能证实,在优选条件下(细砂、厚度>5 cm),幼蟹所受的胁迫在降低。     

Sex‐related differences in size,function and regeneration of the major cheliped in the hermit crab Pagurus filholi

Pagurus hermit crabs have a well‐developed right cheliped (major cheliped) and in some species the major cheliped of males is longer than that of females. This paper describes sex‐related differences in major cheliped length and regeneration pattern of the major cheliped in the hermit crab Pagurus filholi. We also examined the function of the major cheliped in male–male competition. Major cheliped length of males was longer than that of females in P. filholi. Males regenerated larger chelipeds than females at the first molt after experimentally induced autotomy. Body size growth in males of the regeneration group was less than that in intact males of the control group while there was no significant difference in body size growth of females between regeneration and control groups. Major cheliped length was included in the best model to explain the outcome of male–male competition and thus sexual selection appears to be a causative factor in the sex‐related difference of the major cheliped length. Sex‐related differences in the regeneration pattern may reflect differences in evolutionary pressures on males for large major chelipeds and females for large body size.     

Reproductive pattern and egg development of the deep-sea crab Paromola cuvieri (Brachyura,Homolidae) around the Canary Islands (NE Atlantic)

This work investigated the biology of Paromola cuvieri in the Atlantic Ocean, including reproductive patterns, sex structure, depth distribution and egg development. Females were more abundant than males, mainly at depths greater than 600 m. Males were dominant in size classes larger than 110 mm carapace width (CW), especially over 120 mm, for which only males were observed. The mean length of both sexes decreased with depth. P. cuvieri appears to have continuous reproduction throughout the year. Large females tend to spawn mainly between summer and winter, but smaller females spawn during the summer. Four maturity categories were identified based on the ovary colour, and these categories were histologically validated. Three categories of testes were identified according to their colour and morphology. Sexual maturity was estimated at sizes between 71.6 and 74.0 mm CW for females and at 91.0 mm CW for males. The relative growth showed changes along the ontogeny, at 73.6 mm CW (females) and 91.3–92.2 mm CW (males). Egg development appears to not be completely synchronous, and two colour patterns can be observed simultaneously. Four stages of egg development were found: eggs undivided and fully filled with yolk in orange egg masses; eggs with a free region of yolk visible in orange or red egg masses; embryos with slightly pigmented, crescent-shaped eyes in brown egg masses; and embryos with visible pigmented structures, enlarged eyes, segmented appendages and abdomen in brown egg masses. The mean number of external eggs carried by females in stage I was estimated to be 315,753±19,267. Three species of barnacle were observed on the exoskeleton: Poecilasma aurantia, Poecilasma crassa and Heteralepas microstoma (Cirripedia, Thoracica).     

Habitat preference and locomotive traits of the Japanese mitten crab Eriocheir japonica within the stream unit of the freshwater river environment

Preferences by the Japanese mitten crab Eriocheir japonica of three mesoscale environments within a river unit (pools, riffles and runs) were investigated in a freshwater river by pot fishing and hand-net collecting during the fall. In addition, E. japonica’s life modes during each growth stage were elucidated by comparing data obtained by hand-net collecting into three size groups (20 mm < CW, 20 mm ≤ CW < 40 mm, and 40 mm ≤ CW). Both the number of crabs caught per pot and per 10 m stretch sampled along the river by hand-net collecting indicated that mean catch values varied in the following manner runs < pools < riffles, and that this variation was large in riffles and small in pools and runs. Only large crabs of 40 mm ≤ CW could be collected by pot fishing; in contrast, small juveniles were only caught by hand-net collecting. Results of the hand-net collecting indicated that small juveniles (CW < 20 mm) preferred to be in riffles, while the mid-size class crabs (20 – 40 mm CW) presence in the riffles decreased and once they became adults (40 mm ≤ CW) they chose to be in pools. In all size classes, runs were not a preferred choice. These results suggest that the small juveniles are oriented by their positive rheotaxis. Their movement is probably affected by flow regimes, creating large variations in their density in the riffles. In contrast, adults can freely move within a stream unit and use pools as their resting site, thus they are steadily captured in large numbers with a small variation in the pools by pot fishing. However, the adults can actively wander around riffles and runs, searching for animal food, and are attracted to the riffles by bait odors depending on the water flow. Therefore, the most effective catching method is to set the pots in riffles when the river is swollen.     

日本绒螯蟹放流群体的遗传变异

利用水平电泳方法研究日本绒鳌蟹放流群体（新县的三面川和岛根县的高津川的遗传变异。计检测了15种同工酶,22个位点。两河川日本绒螫蟹的多态座位比例及平均杂合度）利力0．273和0．020,0．227和0．022,与其他种群基本一致。另外,两群体其他相近两自从群体的根井遗传距离为0．001。结果表明：到目前为止,尚未见到放流群体的遗传变异的明显减少。     

利用湖泊内源性饵料饲喂中华绒螯蟹(Eriocheir sinensis)的养殖与净水效应

在东太湖网围养殖区进行不同放养密度(3000、6000、9000只/hm²)饲喂太湖水草与螺蛳的中华绒螯蟹(Eriocheir sinensis)养殖实验,各处理组均设投喂鱼肉与玉米等外源性饵料的对照,以获得不同养殖模式对中华绒螯蟹生长、品质及养殖水环境的影响,以及利用浅水草型湖泊内源性饵料养殖中华绒螯蟹的适宜放养密度.结果表明,规格和增肉倍数虽然均显著小于相同养殖密度下投喂外源性饵料的对照组,但是各实验组商品蟹体重均能达到150 g;低密度养殖组(3000只/hm²)的产量与对照组无显著差异,其余两组显著低于对照组;而低密度养殖组的河蟹回捕率显著低于对照组,其余两组则无显著差异.实验养殖区水质状况优于投喂外源性饵料的对照区,其TP含量显著低于对照区,投喂外源性饵料会增加水体N、P含量,导致水体富营养化.投喂太湖水草与螺蛳等内源性饵料进行中华绒螯蟹养殖不但可以有效地抑制浅水湖泊水草的疯长,还可通过中华绒螯蟹收获从水体输出N、P营养盐,起到净化水质的作用.不同密度养殖的生态与经济效益对比结果显示,利用草型湖泊内源性饵料养殖中华绒螯蟹的适宜放养密度为6000只/hm²左右.     

长江中华绒螯蟹的资源与养殖现状及其种质保护

根据中华绒螯蟹的资源及其养殖现状,分析了我国目前各水系河蟹的种质变异及其河蟹幼蟹培育和成蟹养殖中存在的问题,针对中华绒螯蟹 种质保护的要求,就防止河蟹幼蟹性早熟及和蟹养殖的遗传污染与种质混杂等问题,提出了对长江河蟹品质保持的初步设想。     

山东半岛近岸海域蟹类群落结构特征的研究

根据2014年8月和10月、2015年2月和5月使用单船底拖网进行的4个航次调查数据,对山东半岛近岸海区的莱州湾及渤海南部、山东半岛北部和山东半岛南部等3个海域蟹类种类组成、时空分布、优势种、群落结构稳定性等群落结构特征进行了研究。结果显示,4次调查共捕获蟹类20种,隶属于10科17属,其中日本蟳（Charybdis japonica）和三疣梭子蟹（Portunus trituberculatus）是主要经济种类,其余均为小型饵料型蟹类;海区内主要的优势种为双斑蟳（Charybdis bimaculata）、日本蟳（Charybdis japonica）和三疣梭子蟹（Portunus trituberculatus）,3个海域优势种季节更替均十分明显;莱州湾及渤海南部和山东半岛南部2个海域生物量季节变化明显,春季较低,在夏季达到峰值,夏季到冬季呈降低趋势,山东半岛北部海域生物量季节变化不明显。3个海域蟹类群落物种多样性指数（H'）、丰富度指数（D）及均匀度指数（J'）均处于较低水平,并有一定的季节变化;水深、水温和盐度是影响蟹类群落结构的重要因子,渤莱沿岸流、黄海暖流和黄海冷水团通过影响海域内水温与盐度影响蟹类群落结构。研究表明山东半岛近岸海区蟹类以小型饵料型蟹类为主,蟹类多样性水平较低,优势种更替明显,群落季节更替指数较高,季节间迁移频繁,蟹类群落不稳定。     

三疣梭子蟹(Portunus trituberculatus)冻藏过程中肌肉蛋白质生化特性的变化

以三疣梭子蟹肌肉基本营养成分、蛋白质组成、肌原纤维蛋白含量、Ca~(2+)-ATPase活性、总巯基含量、二硫键含量以及肌原纤维蛋白的SDS-PAGE分析作为指标,研究了三疣梭子蟹在不同冻藏温度下肌肉蛋白质生化特征的变化。结果表明,三疣梭子蟹是典型的高蛋白食品;随着冻藏时间的延长,水溶性蛋白含量先增加后减少,盐溶性蛋白和不溶性蛋白含量逐渐减少,碱溶性蛋白含量逐渐增加;肌原纤维蛋白含量、Ca~(2+)-ATPase活性、总巯基含量随着冻藏时间的延长,均呈现下降趋势,而二硫键含量则呈上升趋势,且–20℃和–40℃两组之间差异显著(P0.05)。SDS-PAGE分析结果表明,组成肌原纤维蛋白的各种蛋白质均有不同程度降解,且–20℃比–40℃组降解更明显。因此,–40℃冻藏对梭子蟹肌肉蛋白质生化特性的影响较小。     

Physiological energetics of the fourth instar of Chinese horseshoe crabs (Tachypleus tridentatus) in response to hypoxic stress and re-oxygenation

Hypoxia associated with eutrophication is a potential threat to the Chinese horseshoe crab Tachypleus tridentatus which inhabits intertidal sand flats in Asia. This study investigated the effect of dissolved oxygen level (DO) (6, 4 and 2 mg O₂ l⁻¹) on the physiological energetics in the juvenile T. tridentatus. They were exposed to various oxygen levels for three days and then transferred to normoxia for three days to examine the recovery from low oxygen stress. Feeding rate, respiration rate and scope for growth were reduced at lower DO levels while absorption efficiency and excretion rate were independent of DO levels. Although full recovery of the physiological responses and scope for growth from hypoxis stress was observed when normoxia resumed, their long term survival in suboptimal habitats with frequent occurrence of hypoxia deserves a close monitoring as hypoxia may be even more common in future in a warming world.