影响萼花臂尾轮虫种群增长的生态学因子的研究：温度和饵料密度的影响

本文研究了在5个温度梯度和4个饵料密度的20个试验组合下，萼化臂尾轮虫（Brachionuscalyciflorus）的种群增长规律。结果表明，该轮虫实验种群的最适温度范围是28—32℃，临界高温是36－40℃；最适饵料密度是45－65×106个／ml。这两个生态因子的最佳组合是温度32℃，饵料密度为65×106个／ml，此时，种群达最大密度3．4×103个／ml。     

Molecular identification of bloom-forming species Phaeocystis globosa(Prymnesiophyta)and its dispersal based on rDNA ITS sequence analysis

Abstract-The colony-forming Phaeocystis species are causative agents of dense bloom occurrences incoastal waters worldwide. It is difficult to separate them because of the different morphologies associatedwith their colonial stages. In this study we applied molecular approaches to analyze the genetic variationof Phaeocystis globosa and Phaeocystis pouchetii from several geogrtaphic regions, and to assist in tracingthe dispersal of bloom-forming Phaeocystis species in coastal waters of China. The Sequences of the inter-nal transcribed sacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystisstrains were determined. Sequence comparison shows that P.globosa was the most divergent toP. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences ex-isted between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearlyshows that the species isolated from the southeast coast of China is identified as P. globosa rather thanP. cf. po     

热带海洋珍珠贝类立克次体(RLO)病研究 Ⅷ.养殖大珠母贝贝苗养成期群体RLO病流行病学研究

大珠母贝人工养殖过程中,幼贝死亡是一个严重的问题。1993年11月至1995年5月,采用现场调查、实验室研究和数理分析相结合的方法,在海南省临高县新盈珍珠贝养殖场自然海区吊养的A,B,C3批养殖贝和陵水新村珍珠贝养殖场养殖池吊养的D批养殖贝的发病和死亡情况进行了流行病学凋查.结果显示,大珠母贝幼贝群体的死亡是以暴发性方式发生,幼贝群体的大批量死亡高峰一般发生在4～6月龄期,8个月以后随着龄期的延长,死亡率显著降低.幼贝群体死亡率与贝体平均体长的关系是4cm以下的贝体死亡率较高,1～3cm阶段处于死亡高峰期内,5cm以上的贝体死亡率显著降低.大珠母贝幼贝群体的死亡率与类立克次体感染(即RLO平均感染严重度指数SI)之间存在显著的相关性,在RLO感染的高峰之后或当中部相应伴随贝群体死亡率的高峰,随着RLO感染的降低,贝群体的死亡率也明显降低。在4批养殖贝群体中,仅在A批养殖贝群体样本中发现有少量纤毛虫寄生(感染率为87.5%,感染强度为3.56个/10倍物镜).这几批幼贝群体的死亡与海水温度、盐度之间均无相关性。     

Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.     

Identification of Epinephelus malabaricus and Epinephelus coioides using DNA markers

1 IntroductionBothEpinephelus malabaricusandE. coioidesbelong to the genusEpinephelus(Perciformes: Ser-ranidae: Epinephelinae).They are warm water reeffishes that have similar distribution ranges as well assimilar morphological characteristics, which have…     

黄东海间遗传连通性多物种案例分析与启示

黄东海是太平洋重要的边缘海,拥有丰富的海洋生物多样性资源,但在气候变化及人类活动的双重胁迫下,黄东海面临生物多样性衰退等问题,亟需加强保护。保护区网络被认为可有效防止或减缓人类活动和气候变化对生物多样性的负面影响,但连通性作为保护区网络的重要参数却很难直接测量。因此,本研究希望通过梳理已发表的黄东海间遗传连通性文献来揭示黄东海区域内的保护区是否形成网络。通过搜集已发表的137篇文献,共获得175个研究案例。超过半数的案例(65.71%)认为黄东海之间具有显著的遗传分化,这也意味着跨生态区群体间具有较低的遗传连通性。在总结影响因素时发现长江冲淡水、生境异质性、空间距离、进化过程、生活史(较短的浮游幼体期)、洋流等6个因素阻碍了黄东海群体间的遗传连通性,而洋流、生活史(较长的浮游幼体期)等2个因素促进了遗传连通性。进一步分析发现黄东海群体间的遗传连通性与空间距离呈显著的负相关,而与浮游幼体期呈正相关。因此,根据本研究结果,建议未来保护区网络建设应针对黄、东海生态区分别构建网络,在网络设计时应考虑保护对象的扩散能力及保护区间的空间距离。本研究不仅为构建涵盖黄东海的海洋保护区网络提供理论基础,...     

甘肃省舟曲特大泥石流的遥感影像特征及古泥石流遥感识别的地质意义

2010年舟曲特大泥石流是舟曲地区第四纪以来泥石流最新暴发期内强烈活动的体现,所造成的人员伤亡及财产损失堪称世界泥石流灾害史之最。准确分析该地区泥石流的空间分布规律及预测未来泥石流暴发时间可为泥石流的防灾救灾提供科学的依据,其首要任务就是利用一种先进技术对不同时期的泥石流堆积物的空间分布进行快速、准确的识别。遥感技术具有数据获取量大、空间分辨率高及快速等方面的独特优势,可在舟曲特大泥石流及不同时期古泥石流堆积物识别中发挥重要作用。在高分辨率的航空遥感影像上,舟曲特大泥石流具有明显的光谱特征,可用于准确圈定泥石流灾害的范围; 泥石流堆积物在沟谷两侧形成的侧碛堤及在堆积区末端形成的扇形地等形态特征也是识别泥石流的另一个重要标志。舟曲县城东南瓦厂村南及马莲坪村西南白龙江支流沟中的垄岗状堆积物和沟口处的扇形地堆积体在光学遥感影像上具有典型的古泥石流堆积的形态特征,雷达遥感的后向散射系数对比分析和对堆积物剖面的沉积特征分析也证明古泥石流的存在。研究结果表明,通过多期次、多空间分布的现代和古泥石流的对比,不仅可以揭示舟曲地区第四纪以来泥石流发育历史并间接反映地质尺度上气候变化特点,还可为未来泥石流的易发性分析提供数据支撑,因此泥石流堆积物的遥感直接识别具有重要的地质意义。