海水盐度对牙鲆仔稚鱼的生长、存活率及白化率的影响

１９９３年１１月２０日自鹿儿岛养殖业购得自然产卵获得的牙鲆受精卵，经空运、陆驼至福冈的九州大学附属水产试验所，仔鱼翌日孵出，３ｄ后开口。以此材料观察研究试验室条件下不同海水盐度对牙鲆仔稚鱼的生长，存活率及白化率的影响。试验分４组，盐度分别为：１组（自然海水对照组），３０．５－３１．８；２组，２６．０：３组，２１．０；４组，１６．０。每组设２个重复，使用１００１透明玻璃钢水槽，每槽放２０００尾刚开口     

The Effect of Human Exclusion on the Population Structure of Key-Hole Limpets Fissurella crassa and F. limbata on the Coast of Central Chile

Abstract. We describe the basic ecology of two key-hole limpets, Fissurella crassa and Fissurella limbata , which are heavily harvested by coastal shellfishermen (mariscadores) at the rocky intertidal of central Chile. The ecological role of human predation on these species was assessed. Anthropic exclusion from a stretch of the rocky intertidal at Las Cruces (non-harvested area) for about two and a half years resulted in changes of the densities and size distributions of these fissurelid species as compared to populations in control zones (harvested area). Differential human predation generates, on key-hole limpets, responses of different intensities related to the accessibility of man to the mesohabitats (sheltered platforms, exposed platforms and vertical walls) and fringes of the intertidal (mid-low intertidal and Lessonia nigrescens fringe). Thus, size distributions and mean densities of both fissurelids differ widely between the harvested and non-harvested areas on both platform habitats but not on vertical walls. In the mid-low intertidal fringe, differences are greater than in the less accessible L. nigrescens intertidal fringe. Moreover, we discuss the way in which other species, directly harvested by mariscadores (i. e., the mollusc Concholepas concholepas) can affect the keyhole limpet populations of central Chile rocky shores. Finally, we highlight the ecological role of these limpets on the dynamics of intertidal ecosystems and how they can modify community landscape.     

Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.     

应用六氯化苯测年法对厦门港现代沉积速率的测定

岩芯柱样用静力岩芯采样器于1986年采自福建省厦门港一九龙江口海域。通过对这些岩芯柱样中六氯化苯(BHC)的垂直分布状况和生物扰动类型的分析,应用 S_R=D_(BHC-D_(mix)/Y的计算模式,获得了这些岩芯的现代沉积速率数据。将这些数据、方法与原理完全不同的~(210)Pb放射性同位素测年法同岩芯测年数据比较,其结果在数据和数量级上有一定可比性。初步认为,再经进一步探索和改进,BHC测年法有可能应用于近岸河口港湾几十年来现代沉积速率的测定而与其它测年法互为补充。     

厦门港真刺唇角水蚤的生长率

本文以实验室培养为主,结合自然种群调查,观测厦门港真刺唇角水蚤各发育阶段体长、体重的增长状况,并研究了其生长率与温度、发育期的关系。     

虾肤诱食剂改善南美白对虾植物蛋白日粮的作用

用900尾平均体质量为0.106 g/尾±0.012 g/尾的南美白对虾(Peneausvannamei)进行虾肽改善植物蛋白日粮试验,结果表明:(1)大豆粕、杂粕组南美白对虾的成活率显著高于鱼粉组(P<0.05);(2)大豆粕、鱼粉组南美白对虾相对生长率、饲料系数、虾体组成相似,杂粕组最差,且与大豆粕、鱼粉组有显著性差异(P<0.05)。结果表明,在南美白对虾日粮中虾肽作为营养性诱食剂可显著改善植物蛋白的适口性,提高南美白对虾对大豆蛋白的的消化吸收功能,达到与鱼粉组相同的养殖效果,从而明显降低南美白对虾日粮成本。     

不同卵密度对太平洋牡蛎三倍体诱导效果影响的研究

本实验主要研究用70mg／L浓度的6－MDAP（二甲基氨基嘌呤）对太平洋牡蛎的受精卵进行三倍体诱导，研究受精卵的密度对诱导效果的影响，以及诱导后受精卵的孵化率和畸形率。实验结果表明：当受精卵的密度为4万个／ml时三倍体率最高，相应的孵化率最高，综合来看，4万/ml的卵密度是生产上较好的受精卵处理密度。     

鲜水河断裂带炉霍段的水平运动及地震的重复性研究

本文讨论了鲜水河活动断裂带炉霍段的水平断错、古地震遗迹与地震重复间隔等问题。晚更新世以来断裂的平均滑动速率为13毫米/年。全新世中期以来大震的重复间隔时间小于600年