Genetic diversity and population structure of Eleutheronema rhadinum in the East and South China Seas revealed in mitochondrial COI sequences

Eleutheronema rhadinum is a potential commercial fisheries species and is subject to intense exploitation in China. Knowledge on the population structure of E. rhadinum in Chinese coastal waters, which is important for sustainable exploitation and proper resource management, is lacking. In the present study, the genetic diversity and population structure of E. rhadinum were evaluated using a 564-base pair fragment of the mitochondrial cytochrome c oxidase subunit I （COl） gene. A total of 76 specimens were collected from three localities around the East （Qidong and Zhoushan） and South China Seas （Zhuhai）. Among these individuals, nine polymorphic sites were detected and 1 l distinct haplotypes were defined. High levels ofhaplotype diversity （h=0.759i0.035） and low levels of nucleotide diversity （re=0.001 98i0.003 26） were observed in these populations, Hierarchical analysis of molecular variance （AMOVA） indicated that 96.72% of the genetic variation occurred within the populations, whereas 3.28% occurred among populations. No significant genealogical branches or clusters were recognized on the neighbor-joining tree. Intra-group variation among populations was significant （~0~t=0.032 85, P〈0.01）. These results suggest that E. rhadinum populations in the East and South China Seas have developed divergent genetic structures. Tests of neutral evolution and mismatch distribution suggest that E. rhadinum may have experienced a population expansion. The present study provides basic information for the conservation and sustainable exploitation of this species.     

面向防震减灾的人口数据空间化研究——以2007年宁洱地震灾区为例

入口是地震灾害的重要受灾体,准确的入口空问分布信息是防震减灾工作的重要依据.本文借助地理信息系统,将人口统计数据与高分辨率遥感数据相结合,应用基于居民地的人口数据空间化方法,模拟人口空间分布.首先根据城市人口—面积异速生长模型的分形几何意义,推导出城乡人口一面积统一模型;进而以2007年宁洱地震灾区为例,在建立居民地分类体系和遥感解译标志的基础上,目视解译获得准确的居民地信息;最后应用城乡人口—面积统一模型获得网格人口密度矢量数据.经检验,本文的结果达到了较高的精度.同时在人口数据空间化完成的基础上,以地震受灾人口估算为例,探讨了人口数据空间化在防震减灾中的应用.研究结果表明,基于网格人口矢量数据的受灾人口估算结果更能客观反映地震灾情,可以为防震减灾和应急救援工作提供可靠的依据.     

Evidence of the restricted gene flow within a small spatial scale in the Japanese common intertidal goby Chaenogobius annularis

Elucidating the scale of gene flow among populations is an important challenge for understanding the ecological dynamics and local adaptation of marine organisms. We assessed whether gene flow is restricted even at a small spatial scale in the Japanese common intertidal goby Chaenogobius annularis, using highly polymorphic DNA markers, involving the mitochondrial DNA (mtDNA) control region and 15 microsatellite DNA (msDNA), because past ecological studies have suggested low dispersal ability for rocky intertidal fishes. We found significant heterogeneities between four neighboring local populations by both mtDNA and msDNA analyses. In addition, no genetic heterogeneity was detected by either method across generations within a population; it was considered that such genetic differentiation is retained across generations and that the gene flow of this species is restricted to within a radius of a few kilometers. This is the first report showing a clear genetic subdivision in rocky intertidal fish.     

Changes in vertical distribution and aggregative behaviour in response to population density for larval sea urchins (Strongylocentrotus droebachiensis) and sea stars (Asterias rubens)

We examined the effects of the presence of larval conspecifics on larval vertical distribution of four‐arm and six‐arm plutei of Strongylocentrotus droebachiensis and bipinnaria of Asterias rubens, in laboratory experiments, by manipulating population density. Larvae were introduced to experimental columns (10 × 10 × 30 cm) at one of two or three population densities (S. droebachiensis: 0.1 and 10 larvae·ml^?1; A. rubens: 0.1, 1 and 10 larvae·ml^?1). Subsequent changes in vertical distribution were determined from images of the larvae in the columns illuminated by a 532‐nm laser and captured by a high‐resolution camera. Larvae of both species were found higher in the water column in the high than in the low and intermediate population densities. The relationship between vertical swimming velocity and nearest neighbour distance (NND) was measured for four‐arm plutei of S. droebachiensis, and used to determine a range in distances among individuals that may affect potential interactions. The variation in swimming velocity decreased with increasing proximity below a threshold distance among individuals of 10 mm, which was greater than the estimated distance in the high population density. We suggest that the increased larval aggregation near the water surface under high population density is the result of a behavioural response to conspecifics.     

Genetic variability of the blue and red shrimp Aristeus antennatus in the Western Mediterranean Sea inferred by DNA microsatellite loci

Genetic variation at eight microsatellite loci was studied in nine populations of the blue and red shrimp Aristeus antennatus to investigate whether distinct stocks are present in the Western Mediterranean Sea. A high level of gene flow and no evidence of genetic partitioning were discovered. No significant variation was found (F_ST = 0.00673, P-value = 0.067) even when shrimps from exploited and those from deep-water unexploited grounds were compared. No evidence of reduction or expansion of population size in the recent past was found, as indicated by the bottleneck and interlocus g-tests. Our results are consistent with previous studies using mitochondrial gene methods and allozymes, indicating that, for this species, extensive pelagic larval dispersal and adult migration are probably responsible for the genetic homogeneity observed. In particular, due to a different bathymetric distribution of males and females, reported to be associated with different water masses and hence with possible differential dispersal capacity between sexes, the hypothesis of sex-biased dispersal was tested. Mean values of corrected assignment indices and mean relatedness values were higher for males, suggesting that females are the more widely dispersing sex. Molecular assessment of A. antennatus from the Western Mediterranean provides data of biological and evolutionary interest for the successful management of such a highly valuable fishery resource.     

海洋围隔生态系中浮游动物的研究

2002-05-20-26在东海进行小尺度船基浮游生物生态系围隔实验,研究浮游动物的群落结构、组成与环境之间的关系,探讨了优势种群的自然繁殖发育期。结果表明,浮游动物的种类组成较简单,优势种明显。浮游动物生物量、丰度及优势种群丰度三者的变化呈逐渐增加趋势。在实验期的1～5d,小拟哲水蚤的无节幼虫和早期幼体占绝对优势,该自然种群的丰度不断增加;随时间推移,无节幼虫逐渐发育为桡足幼体,而后又逐渐发育为成体。因此,本次实验期是实验海区优势种小拟哲水蚤种群的自然繁殖发育盛期。     

中国省域犯罪率影响因素的空间非平稳性分析

收入差距和流动人口是影响犯罪率的两个重要因素, 以往研究基于OLS模型, 在假设地域空间为均质的前提下分析其对犯罪率的影响, 但现实世界的空间单元往往难以满足“均质”的假设, 多数表现为“空间异质”。以OLS计量空间异质会造成计量结果出现偏差, 同时无法了解不同空间单元的不同影响。而地理加权回归模型通过将空间结构嵌入线性回归模型中, 很好的解决了空间异质的计量问题。利用地理加权回归模型研究2008 年中国大陆省域单元犯罪率的影响因素, 结果表明:① 犯罪率的影响因素表现出空间非平稳性, 流动人口与犯罪率显著相关, 但各个省份相关程度并不相同, 影响关系随空间位置变化而变化;② 地理加权回归模型的计量精度和拟合度比OLS模型有大幅提高     

1996-2011 年南非人口空间分布格局与演变特征

南非是中国在非洲的最大投资目的地与经贸合作伙伴, 特别在基础设施建设方面发展迅速。南非人口空间分布研究对开展中南合作具有重要意义。本文通过收集整理南非历年来的全国人口普查资料统计数据, 运用空间自相关模型、不均衡指数、人口重心模型和偏移—分享模型, 分析了南非人口的空间分布格局和演变特征。研究结果表明, 南非人口分布具有显著的“东高西低”的空间格局, 呈现出以工业化程度较高的大都市区和港口城市为中心的“多核心”集聚的分布特征;空间自相关分析得出人口高集聚区主要分布在大都市区和沿海港口城市, 低密度集聚区主要集中分布在中西部地区;从变化趋势上来看, 1996-2011 年间人口分布不平衡趋势不断加强, 人口重心向西北方向移动;偏移—分享分析表明, 南非人口增长速度先后经历了从1996-2001 年快速增长到2001-2007 年缓慢增长, 再到2007-2011 年高速增长的“高—低—高”增长阶段;省域层面上看, 豪登省和西开普省一直是南非人口集聚较强地区。     

贵州乌蒙山区资源相对承载力的时空动态变化

贵州乌蒙山区是国家划定的18片贫困区之一,随着人口增长和社会经济发展的加速,山区人地资源矛盾日趋突出,已严重阻碍了乌蒙山区社会经济的可持续发展。运用并改进了相对资源承载力的研究思路和计算方法,研究了1995~2006年贵州乌蒙山区自然资源、经济资源、生活水平的相对承载力和综合承载力的时空动态变化过程。对于认清和把握乌蒙山区资源承载力变化规律,完善山区资源环境承载力研究成果具有重要指导意义。研究表明:贵州乌蒙山区人口严重超载,而承载人口的主要资源为土地资源,经济资源和生活水平承载力偏低;相对资源承载力空间差异显著,绝大多数县域综合承载力均处于超载状态。     

基于多模型的西宁市人口规模预测

人口是反映国情、国力基本情况的重要指标,是区域研究所必须考虑的重要因素之一。合理、准确地预测城市人口规模,是城市与区域规划中首先要考虑的基本问题,也是保证规划科学性与可实施性的关键性前提。以西宁市2000-2011年历年总人口为样本数据,分别构建了一元线性回归模型、马尔萨斯模型、logistic模型及GM（1,1）模型,并进行模型检验。结果表明：（1）模型均通过模型精度检验且精度较高,GM（1,1）模型拟合度最高,均误差达到0.004%,马尔萨斯模型拟合度最低,为-1.440 8%;（2）分析模型预测精度差异产生原因及适用性,表明深入、准确地分析样本数据特征,恰当选择分析方法对于控制人口预测精度尤为重要。由于西宁市2000-2011年人口样本数据在2005及2009年数据存在波动性,破坏了其与一元线性回归模型及马尔萨斯模型的拟合度,导致在4种模型中,Logistic及GM（1,1）模型预测精度较高,而GM（1,1）模预测精度最高,所以采用GM（1,1）模型进行西宁市人口预测,得到西宁市人口预测的最终结果：2012年西宁市总人口将达到225.89×10⁴人,2015年将达到233.39×10⁴人,2020年将达到246.37×10⁴人。从结果看,未来9 a西宁市人口将呈现持续平稳增长的态势,但随着时间推进人口增长速度将逐渐下降。