一种海洋浮游植物定量研究分析方法——Utermhl方法的介绍及其改进

对国际上通用的浮游植物定量生物学基础研究方法———Uterm¨ohl方法的应用进行了介绍 ,并对此方法的不足之处和误差来源进行了分析。在此基础上 ,对误差的统计方法提出进一步改进。同时 ,指出了我国现有海洋调查中浮游植物定量研究方法的不足之处 ,建议以Uterm¨ohl方法替代网采方法作为今后中国海洋浮游植物定量生物学研究的基本手段     

福建福宁湾无机氮含量的变化特征及其对浮游植物的影响

本文根据2002-2003年福宁湾海域的监测资料对NO2-N、NO3＋N、NH4-N三态DIN的变化特征和相互关系进行分析，并分析其对浮游植物生物量的影响．研究表明：NO3-N、DIN含量均呈冬季最高、夏季最低，NO2-N含量秋季最高，冬季最低；福宁湾海域赤潮高发期水体DIN中三氮所占比例与年平均相比变化最大的是NO3-N，其次是NH4-N，NO2-N最小；NO3-N、DIN与盐度均呈明显负相关；NO3-N与DIN呈明显正相关；NO3-N是福宁湾海域DIN的主要存在形式；三态氮中仅NO3-N为福宁湾海域浮游植物生长限制因子；浮游植物对DIN的吸收主要以NO3-N形式为主。     

1999年7月胶州湾东部赤潮生消过程生态环境要素分析

根据 1 999年 7月 2 3 - 2 4日胶州湾东部赤潮发生海区的观测资料 ,结合同年 6 ,7,8月3个航次的数据 ,分析了赤潮前后水体浮游植物群落、叶绿素 a和营养盐等要素的变化特征。结果显示 ,本次赤潮为短角弯角藻 (Eucampia Zoodiacus)赤潮 ,总生物密度高达 1 .6× 1 0 10个 /m L。赤潮发生时 ,叶绿素 a浓度范围为 2 8.8～ 3 6 .0 mg/m3 ;温度、盐度变化不大 ,DO,p H值升高 ,营养盐浓度处于较低水平 ,其中 P降幅最大 ,N/P值则急剧上升 ,峰值为 2 4 7,P被认为是本次赤潮的限制因子 ;NO2 - N/NO3 - N的值变化很大 ,高于正常值。赤潮消退 ,各要素恢复正常     

Effects of long-term sample preservation on flow cytometric analysis of natural populations of pico-and nanophytoplankton

The effects of long-term preservation on flow cytometric parameters of natural oceanic populations of pico-and nanophytoplankton have been examined. Populations collected at oligotrophic subtropical and subarctic locations in the North Pacific were fixed with glutaraldehyde and frozen in liquid nitrogen, according to Vaulot et al. (1989). During six months’ storage, chlorophyll red fluorescence declined in all the groups examined, while forward light scatter was enhanced in Synechococcus and Prochlorococcus, and weakened in nanoeucaryotes. Cell loss was not significant except for Synechococcus. Caution is required when analyzing flow cytometric data of samples stored for more than a month.     

On Conditions of Phytoplankton Blooms in the Coastal Waters of the North-Western East/Japan Sea

Seasonal changes of abundance of the main phytoplankton groups of species (diatoms, dinoflagellates, chrysophytes, small flagellates and cryptophytes) and a set of environmental parameters were investigated in coastal and preestuarine waters of Peter the Great Bay (East/Japan Sea) in May-October of 1998 and 1999. Three periods of mass development were revealed: spring, summer and autumn blooms, with successive change of species. The conditions favourable for each group of species were determined. Driving mechanisms of the succession include nutrients transport through seasonal pycnocline by turbulent mixing, terrestrial nutrients supply by monsoon floods, nutrients supply by upwellings, and light control by the thickness of upper mixed layer. Summer succession could be explained by a simple SST-MLD diagram similar to Pingree S-kh diagram with sea surface temperature as indicator of stratification (S) and mixed layer depth as indicator of light availability (kh).     

罗源湾和闽南—台湾浅滩海域浮游植物的光合参数

本工作采用~(14)C示踪法,于1987～1989年现场测定了罗源湾和闵南-台湾浅滩渔场浮游植物碳同化速率。结合叶绿素和细胞含碳量的测定,描述了浮游植物光合参数的季节变化,探讨温度对同化系数和碳比积累速率的影响。结果表明,罗源湾碳比积累速率平均为0.64d~(-1),闽南-台湾浅滩渔场碳比积累速率平均为0.85d~(-1),这反映了两个海区初级生产力的差别。温度对碳比积累速率的影响可以采用Goldman和Carpenter的模式来描述。     

Ecological characteristics of phytoplankton community from eutrophic waters in the Maluan Bay

I~IOWTheMaluanBayisashallowandmOStlyendedwatertheywhichisisolatedfromwesternXiamenhaforbyaseawall.Thebayhaslongbenboldingagreatdealofagriculturalandindustrialwaterandmunicipalsewage.Moreover,aquacultureindustryhasbeendeveloppedinal~aceinthebayinrecen...     

Particulate n-alkanes and fatty acids in the Changjiang river system

Particulate samples were collected from the Changjiang river system during a flood period, in May 1997, and POC, stable isotope and lipids associated with particles were examined. Results showed the decrease (0.84% ～ 1.88%) of organic carbon content from the upper reaches to the estuary.δ13C values of particulate organic carbon was in the range of -24.9×10-3 to -26.6×10-3, which were close to the isotopic signature of continental C3 vegetation. Total particulate n-alkanes concentrations varied from 1.4 to 10.1μg/dm3,or from 23.7 to 107μg/g of total suspended matter. Fatty acids were present in all the samples, from 1.4 to 5.4μg/dm3, with saturated and unsaturated straight-chain and branched compounds in the carbon number range from C12 to C30. Both δ13C and the ratio of carbon content to nitrogen content indicate the predominance of terrestrial inputs (soil organic matter) among the particles. The biomarker approach has been used to identify the relative portion of terrigenous and autochthonous fraction in the particulate samples. The distribution of fatty acids suggests a striking phytoplanktonic and microbial signal in most particle samples. The terrestrial alkanes are used to estimate the contribution of terrestrial inputs along the mainstream.     

Spatial variability of phytoplankton absorption coefficients and pigments off Baja California during November 2002

We have estimated the spatial variability of phytoplankton specific absorption coefficients (a* _ph ) in the water column of the California Current System during November 2002, taking into account the variability in pigment composition and phytoplankton community structure and size. Oligotrophic conditions (surface Chl < 0.2 mg m⁻³) dominated offshore, while mesotrophic conditions (surface Chl 0.2 to 2.0 mg m⁻³) where found inshore. The specific absorption coefficient at 440 [a* _ph (440)] ranged from 0.025–0.281 m²mg⁻¹ while at 675 nm [a* _ph (675)] it varied between 0.014 and 0.087 m²mg⁻¹. The implementation of a size index based on HPLC data showed the community structure was dominated by picoplankton. This would reduce the package effect in the variability of a* _ph (675). Normalized a _ph curves were classified in two groups according to their shape, separating all spectra with peaks between 440 and 550 nm as the second group. Most samples in the first group were from surface layers, while the second group were from the deep chlorophyll maximum or deeper. Accessory photoprotective pigments (APP) tended to decrease with depth and accessory photosynthetic pigments (APS) to increase, indicating the importance of photoprotective mechanisms in surface layers and adaptation to low light at depth. Samples with higher ratios of APP:APS (>0.4) were considered as phytoplankton adapted to high irradiances, and lower ratios (<0.26) as adapted to low irradiances. We found a good relationship between APP:APS and a* _ph (440) for the deeper layer (DCM and below), but no clear evidence of the factors causing the variability of a* _ph (440) in the upper layer.     

Trophic Status of the South-Eastern Baltic Sea: A Comparison of Coastal and Open Areas

Primary production, nutrient concentrations, phytoplankton biomass (incl. chlorophyll a) and water transparency (Secchi depth), are important indicators of eutrophication. Earlier basin-wide primary production estimates for the Baltic Sea, a shallow shelf sea, were based mainly on open-sea data, neglecting the fundamentally different conditions in the large river plumes, which might have substantially higher production. Mean values of the period 1993–1997 of nutrient concentrations (phosphate, nitrate, ammonium and silicate), phytoplankton biomass, chlorophyll a (chl a) concentration, turbidity and primary production were calculated in the plumes of the rivers Oder, Vistula and Daugava and Klaipeda Strait as well as the open waters of the Arkona Sea, Bornholm Sea, eastern Gotland Sea and the Gulf of Riga. In the plumes, these values, except for primary production, were significantly higher than in the open waters. N:P ratios in the plumes were >16 (with some exceptions in summer and autumn), indicating potential P-limitation of phytoplankton growth, whereas they were <16 in the open Baltic Proper, indicating potential N-limitation. On the basis of in situ phytoplankton primary production, phytoplankton biomass and nutrient concentrations, the large river plumes and the Gulf of Riga could be characterized as eutrophic and the outer parts of the coastal waters and the open sea as mesotrophic. Using salinity to define the border of the plumes, their mean extension was calculated by means of a circulation model. Taking into account the contribution of coastal waters, the primary production in the Baltic Proper and the Gulf of Riga was 42·6 and 4·3×10⁶ t C yr⁻¹, respectively. Hence, an annual phytoplankton primary production in the whole Baltic Sea was estimated at 62×10⁶ t C yr⁻¹. The separate consideration of the plumes had only a minor effect on the estimation of total primary production in comparison with an estimate based on open sea data only. There is evidence for a doubling of primary production in the last two decades. Moreover, a replacement of diatoms by dinoflagellates during the spring bloom was noticed in the open sea but not in the coastal waters. A scheme for trophic classification of the Baltic Sea, based on phytoplankton primary production and biomass, chl a and nutrient concentrations, is proposed.