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81.
Response of microbial community to petroleum stress and phosphate dosage in sediments of Jiaozhou Bay,China 总被引:1,自引:0,他引:1
ZHAO Yangguo CHEN Min BAI Jie LI Xinwei Farhana Zulfiqar and WANG Qianli 《中国海洋大学学报(英文版)》2014,13(2):249-256
The dynamic microcosms were used to evaluate the effect of oil spills on microbial ecological system in marine sediment and the enhancement of nutrient on the oil removal. The function and structure of microbial community caused by the oil pollution and phosphate dosage were simultaneously monitored by dehydrogenase activity assay and PCR-denaturing gradient gel electrophoresis (DGGE) techniques. The results indicated that the amount of total bacteria in all dynamic microcosms declined rapidly with incubation time. The number of petroleum-degrading bacteria and the activity of sediment dehydrogenase were gradually enhanced by petroleum in the oil-treated microcosms, while they both showed no obvious response to phosphate dosage. In comparison, phosphate spiked heterotrophic bacteria and they showed a significant increase in amount. DGGE profiles indicated that petroleum dosage greatly changed community structure, and the bacteria belonged to class Deltaproteobacteria, and phyla Bacteroidetes and Chlorobi were enriched. This study demonstrated that petroleum input greatly impacted the microbial community structure and consequently the marine sediment petroleum-degrading activity was enhanced. Phosphate dosage would multiply heterotrophic bacteria but not significantly enhance the petroleum degradation. 相似文献
82.
二苯碳酰二肼光度法是测定微量Cr(Ⅵ)的行业标准方法,以硫酸作为显色介质,酸度要求严格,显色稳定时间短,体系显色后需在10 min~1 h测定完毕,灵敏度较差。本文对标准方法进行改进,将显色介质由硫酸改为磷酸。结果表明,在H3PO4介质中显色剂与Cr(Ⅵ)反应生成的络合物可稳定3 h,方法相对标准偏差(RSD)≤5.25%,加标回收率为99.2%~102.0%,检出限由标准方法的13.6μg/g降至3.8μg/g。建立的方法用于超基性岩标准物质分析,相比于标准方法,稳定性、精密度和准确性均有提高。 相似文献
83.
Sunshading is a powerful tool for the enhancement of edges in images. Given the azimuth and elevation of a source illumination, it calculates the reflectance from a surface which is composed of the data to be interpreted. It is a standard tool used in the interpretation of geophysical potential field data. In the great Oulad Abdoun phosphate basin, inclusions of sterile hardpan — so-called “disturbances” — are hard to detect as they interfere with phosphates. Their resistivity is above 200 Ohmm against 80 to 150 Ohmm for a phosphate-rich mineral. A Schlumberger resistivity survey over an area of 50 hectares was carried out. The geologic setting was successfully modeled on the basis of sunshaded maps of disturbances. A new field procedure was tested to deal with the extended edges of anomalous zones of phosphate deposit disturbances. Phosphate reserves were estimated more reliably. 相似文献
84.
正Lithium is an important element with a wide variety of applications,especially in lithium ion batteries and in the exploration of nuclear energy.Salt lake brine is the most abundant lithium source available in world,comp-rising70%~80%of all known lithium deposits.Lithium can be abstracted from brines with low magnesium content and processed into kinds of lithium compound in Atacama and Hombre Muerto of South America(Nie et al.,2013). 相似文献
85.
湖北省的磷矿资源储量居全国之首,矿床类型以元古宙海相沉积型磷块岩矿床为主,分布集中在五大区域,开发利用条件较好。虽然湖北的磷矿资源勘查近年取得了突破性的进展,但由于资源利用合理度欠缺、磷化工企业规模小、产业链不完善及产业集中度低等原因,对磷矿开发和磷化工产业的可持续发展构成了制约。磷矿资源的合理开发利用对建设磷矿开发及磷化工产业集群、保障国家粮食安全具有重要意义。因此,在勘查部署上,要根据成矿条件、空间富集规律和勘查开发现状,统一部署,分类开展工作;在产业布局上,以宜昌、保康、荆襄、黄麦岭4大磷化工基地为主体,新建鹤峰、黄梅磷肥基地,打造"一圈多点"式产业集群;在产业关联上,新建磷矿产地与现有磷化工基地之间形成以老带新、以新助老、空间互动的产业格局;在产品分工上,支持现有磷化工基地内的骨干企业兼并重组,提高产业集中度。 相似文献
86.
Environmental and land use factors affecting phosphate hysteresis patterns of stream water during flood events (Carpathian Foothills,Poland) 总被引:1,自引:0,他引:1
Flood events play a substantial role in the circulation of catchment phosphate (PO43?). The purpose of the research was to analyze the factors determining PO43? hysteresis patterns (direction and width) during four types of floods: short and long rainfall floods and snowmelt floods on frozen and thawed soil. The research took place in small catchments (forested, agricultural, mixed‐use) in the Carpathian Foothills in Poland. Anticlockwise hysteresis was identified in the forested catchment during short and long rainfall floods. Under the same conditions, the clockwise direction was observed in the agricultural catchment. In the mixed‐use catchment, the direction of PO43? hysteresis loops was various, driven by the share of water flowing from each part of the catchment. For snowmelt floods, the PO43? hysteresis pattern was similar in all the streams studied (usually clockwise). The direction of PO43? hysteresis loops depends on water circulation patterns, which are determined by the different influx times of particular runoff components. The stream recharge mechanism during a flood event is affected both by the factor initiating the event (precipitation, snowmelt) as well as by land use in the given catchment. Hysteresis loop width was determined by the pool of PO43? in a given catchment during the time period immediately preceding a flood event as well as by the quantity of PO43? flushed out of the soil. The greater a catchment's pool of PO43? and the greater its ability to flush PO43? out of the soil and into surface flow, the wider the hysteresis loops. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
87.
88.
Calcium (Ca), phosphorus (P), and chitin are the main components of the exoskeleton of krill. Defluoridation of a solution of sodium fluoride (NaF) using calcium phosphate (Ca3(PO4)2) and chitin as defluoridation agents was studied. Orthogonal experiments were designed to find the optimum reaction conditions for defluoridation, to obtain the maximum defluoridation efficiency and fluoride removal capacity of calcium phosphate and chitin. At the same time, a comparison of the capacity of the two defluoridation agents was made. The results suggest that calcium phosphate has a far greater capability than chitin for the removal of fluoride (F) from water under similar reaction conditions. It is also suggested that Antarctic krill is likely to adsorb fluoride via compounds such as calcium phosphate, hydroxyapatite, and other compounds of Ca and P with the general form (Ca, X)x(PO4, HPO4, Y)y(OH, Z)z, in addition to chitin. 相似文献
89.
Isolation and characterization of phosphate-solubilizing bacteria from seagrass rhizosphere soil 总被引:1,自引:0,他引:1
Upasana Ghosh Ponnambalam Subhashini Elangovan Dilipan Subramanian Raja Thirunavukarassu Thangaradjou Lakshmanan Kannan 《中国海洋大学学报(英文版)》2012,11(1):86-92
Phosphate-solubilizing bacterial strains(6 Nos.) were isolated from the rhizosphere soils of two seagrasses(Halophila ovalis(R.Br.) Hook and Halodule pinifolia(Miki) Hartog) in the Vellar estuary.Experimental studies found that the strain PSSG6 was effective in phosphate solubilization with Phosphate Solubilization efficiency index E = 375 ± 8.54,followed by the strain PSSG5 with Phosphate Solubilization efficiency index E = 275 ± 27.3.Of the 6 strains isolated,the strains PSSG4 and PSSG5 be-longed to the genus Bacillus,and PSSG1,PSSG2 and PSSG3 were identified as Citrobacter sp.,Shigella sp.,and Klebsiella sp.,respectively,by conventional method,and PSSG6 was identified as Bacillus circulans using conventional and molecular methods. 相似文献
90.
Water samples taken at 19 locations in the Mawheraiti River catchment at weekly intervals during 1979–80 were analysed for sodium, magnesium, potassium, calcium, phosphate, nitrate, and ammonium ion concentrations and for electrical conductivity. Seasonal discharge effects were apparent, and lithology and land management practice also influenced solute concentrations. Solute concentrations were generally very low; nitrate and soluble phosphate were rarely greater than 0.05 mg.L‐1 and ammonium was rarely greater than 0.01 mg.L‐1. The 4 major cations (Na, Mg, K, and Ca) usually summed to less than 6 mg.L‐1 much of which was supplied by precipitation. Forest management (clearfelling and slash‐burning) caused significant increases in solute concentrations, but concentrations declined rapidly during succeeding months and approached pretreatment levels after 2–3 years. The higher concentrations associated with forest management in small experimental catchments were rapidly diluted downstream; together with the low natural solute concentrations this suggests that harmful downstream effects of management practices are unlikely under low flow conditions. 相似文献