养殖大菱鲆细菌性红体病病原菌的分离与鉴定

从患有红体病的养殖大菱鲆(Scophthalmus maximus)的肌肉中分离得到一株优势菌并记为H1.经人工注射感染证实H1即为引起养殖大菱鲆红体病的病原菌,其半致死量LD50为2.82×105CFU/mL,而低浓度(1.41×103 CFU/mL)没有造成死亡,但注射部位有脓肿现象,注射相同体积1.5%无菌生理盐水的对照组没有明显的症状,注射部位也无异常.革兰氏染色显示该菌为革兰氏阴性,菌体呈杆状,周生鞭毛.综合该菌的形态、常规生理生化特征和API32E鉴定结果,发现H1与迟钝爱德华氏菌的表性特征非常相似,相似率迭到99.9%.在分子水平上对其16SRNA基因序列的测定,同源性分析,结果表明H1与迟钝爱德华氏菌的亲缘关系最近,相似度达到99%.综合上述研究结果,将该菌株初步鉴定为迟钝爱德华氏茵(Edwardsiella tarda).     

罗源湾船舶溢油风险评价分析

基于罗源湾远期油轮的发展预测,运用概率与数理统计方法计算出罗源湾海域未来 S 年中发生船舶溢油事故的概率基础值为 0.361×10-4/S,进而对罗源湾船舶溢油风险进行预测评价,结果显示:罗源湾海域船舶溢油概率约为每 10 a 一次,每次产生的溢油量约为 57.3 t 原油,经过一个潮周期,油膜面积理论估算值为 0.64 km2.针对罗源湾海域的实际情况,还提出了相应的风险防范措施.     

Distribution and characteristics of volcanic reservoirs in China

About forty productive oil/gas fields hosted in volcanic reservoirs have been found since 1957 in fourteen basins of China. They can be simply subdivided into two groups, the east and the west. Reservoir volcanic rocks of the east group are predominantly composed of Late Jurassic to Early Cretaceous rhyolite and Tertiary basalt, preferred being considered as rift type volcanics developed in the circum-Pacific tectonic regime. Those of the west are Permo-Carboniferous intermediate/basic volcanic rocks, being island-arc type ones developed in paleo-Asian Ocean tectonic regime.     

Volcanically Driven Terrestrial Environmental Perturbations during the Carnian Pluvial Episode in the Eastern Tethys

The Carnian Pluvial Episode (CPE) fingerprints global environmental perturbations and biological extinction on land and oceans and is potentially linked to the Wrangellia Large Igneous Province (LIP). However, the correlation between terrestrial environmental changes and Wrangellia volcanism in the Ordos Basin during the CPE remains poorly understood. Records of negative carbon isotopic excursions (NCIEs), mercury (Hg), Hg/TOC, and Hg enrichment factor (HgEF) from oil shales in a large-scale terrestrial Ordos Basin in the Eastern Tethys were correlated with marine and other terrestrial successions. The three significant NCIEs in the study section were consistently correlated with those in the CPE successions of Europe, the UK, and South and North China. The U-Pb geochronology indicates a Ladinian–Carnian age for the Chang 7 Member. A comprehensive overview of the geochronology, NCIE correlation, and previous bio- and chronostratigraphic frameworks shows that the Ladinian–Carnian boundary is located in the lower part of Chang 7 in the Yishicun section. HgEF may be a more reliable proxy for tracing volcanic eruptions than the Hg/TOC ratio because the accumulation rates of TOC content largely vary in terrestrial and marine successions. The records of Hg, Hg/TOC, HgEF, and NCIEs in the Ordos Basin aligned with Carnian successions worldwide and were marked by similar anomalies, indicating a global response to the Wrangellia LIP during the CPE. Anoxia, a warm-humid climate, enhancement of detrital input, and NCIEs are synchronous with the CPE interval in the Ordos Basin, which suggests that the CPE combined with the regional Qinling Orogeny should dominate the enhanced rate of terrigenous input and paleoenvironmental evolution in the Ordos Basin.     

考虑裂尖塑性区影响的水力压裂缝高计算模型

储层可压性的准确评价是储层压裂设计和压后产能评估的重要前提。目前,采用岩石力学参数进行页岩可压裂性评价取得了较好的现场应用效果。因此,如何准确获取岩石力学参数成为至关重要的问题。通过建立一种基于物理信息约束的神经网络模型,该模型采用物理和数据双驱动,仅使用少量数据就能够实现岩石力学参数的准确预测。为验证模型性能的优异性,采用人工神经网络、随机森林和XGBoost模型与之进行对比。结果表明,物理信息约束的神经网络在少量数据下预测岩石力学参数的平均准确率高于95%,性能远优于其他模型。采用物理信息约束的神经网络预测得到弹性模量、泊松比、抗拉强度和断裂韧性4种岩石力学参数,基于岩石力学参数对储层可压性的影响,建立了基于脆性指数和力学参数的可压性评价方法。最后,以渤海湾盆地沧东凹陷K2段不同储层可压性为例进行验证。结果表明：研究区整体可压性较好,其中,纹层状混合质页岩可压裂指数高于0.7,可压性良好;纹层状长英质页岩、厚层状灰云质页岩和薄层灰云质页岩可压裂指数均处在0.4~0.7,可压性中等。评价结果与实际施工现场各储层日采油量进行对比,证实了可压性智能评价方法的可靠性,该方法可以推广至页岩储层可压性评价工作中。

     

Geophysical investigation using resistivity and GPR methods: a case study of a lubricant oil waste disposal area in the city of Ribeirão Preto,São Paulo,Brazil

Geophysics has been shown to be effective in identifying areas contaminated by waste disposal, contributing to the greater efficiency of soundings programs and the installation of monitoring wells. In the study area, four trenches were constructed with a total volume of about 25,000 m³. They were almost totally filled with re-refined lubricating oil waste for approximately 25 years. No protection liners were used in the bottoms and laterals of the disposal trenches. The purpose of this work is to evaluate the potential of the resistivity and ground penetrating radar (GPR) methods in characterizing the contamination of this lubricant oil waste disposal area in Ribeirão Preto, SP, situated on the geological domain of the basalt spills of the Serra Geral Formation and the sandstones of the Botucatu Formation. Geophysical results were shown in 2D profiles. The geophysical methods used enabled the identification of geophysical anomalies, which characterized the contamination produced by the trenches filled with lubricant oil waste. Conductive anomalies (smaller than 185 Ωm) immediately below the trenches suggest the action of bacteria in the hydrocarbons, as has been observed in several sites contaminated by hydrocarbons in previously reported cases in the literature. It was also possible to define the geometry of the trenches, as evidenced by the GPR method. Direct sampling (chemical analysis of the soil and the water in the monitoring well) confirmed the contamination. In the soil analysis, low concentrations of several polycyclic aromatic hydrocarbons (PAHs) were found, mainly naphthalene and phenanthrene. In the water samples, an analysis verified contamination of the groundwater by lead (Pb). The geophysical methods used in the investigation provided an excellent tool for environmental characterization in this study of a lubricant oil waste disposal area, and could be applied in the study of similar areas.     

安塞油田谭家营油区长2油藏剩余油分布规律及潜力研究

以油藏数值模拟方法为主,同时结合综合地质分析方法对安塞油田谭家营油区长2油藏分小层进行了河流相低渗透储层剩余油成因类型、分布规律及剩余油潜力研究。认为井网未控制型剩余油、注采不完善型剩余油、厚油层顶部滞留型剩余油、层间干扰型剩余油是主要的剩余油成因类型;长21-2储量动用程度高,但剩余储量的绝对数较大,仍然是开发的主力层位,长21-3动用程度很低,但由于存在底水,底水锥进严重,需加强动用程度及开发控制。     

运用因子分析评价山东黑泥湾沉积物重金属污染来源

在对山东黑泥湾沉积物中Cu、Pb、Zn、Cd、Hg、As、Cr和有机质进行分析测试的基础上,运用因子分析评价沉积物中重金属污染来源,结果表明,山东黑泥湾沉积物中存在Pb、Zn和Cd污染;主成分分析中前3个主成分的贡献率分别为44.656%、19.068%和18.098%;认为山东黑泥湾重金属污染主要有3个来源:有机质降解、海上船舶排污和环境背景。     

Unveiling the chemistry of hot protostellar cores with ALMA

High angular resolution mm-wave observations of the Orion-KL region, made with the IRAM Plateau de Bure interferometer (PdBI), reveal the presence of several cores of size 10³ AU, which have distinct spectral signatures. Complex molecules such as ethanol, vinyl cyanide and dimethyl ether show different distributions and their relative abundance varies from core to core by orders of magnitude. The molecular column densities derived in the cores also differ widely from the beam-averaged column densities observed with large single-dish telescopes. Obviously, the predictions of hot core chemistry models must be checked against high resolution observations. ALMA, which allies sensitivity and high angular resolution, will be a key instrument for this type of studies. The PdBI observations were part of a search for interstellar glycine, also carried out with the IRAM 30-m telescope and the Green Bank Telescope. We derive a 3σ upper limit on the column density of glycine of 1×10¹⁵ cm⁻² per 2″×3″ beam in the Orion Hot Core and Compact Ridge. Based on observations made with the IRAM PdB Interferometer, the IRAM 30-m telescope and the NRAO Green-Bank telescope. IRAM is supported by CNRS, MPG and IGN.