帘蛤科4种养殖蛤群体遗传多样性和种间关系的fAFLP分析

利用荧光标记扩增片段长度多态性(fAFLP)技术对文蛤(Meretrix meretrix)、青蛤(Cyclina sinensis)、菲律宾蛤仔(Ruditapes philippinarum)和硬壳蛤(Mercenaria mercenaria)4种帘蛤科贝类的群体遗传多样性和种间关系进行了研究。选择EcoRⅠ/MseⅠ进行酶切,使用6个E 3/M 3引物组合进行扩增,共获得1 096个位点,多态位点比率95.1%,片段长度50~456 bp。其中,文蛤、青蛤、菲律宾蛤仔和硬壳蛤分别得到681,715,702和694个位点,相应的多态位点比率为76.8%,81.7%,83.0%和75.1%,得到17个种特异性位点,可作为4物种特征标记。分析了群体遗传相似系数和遗传多样性指数以及种间遗传相似系数。结果表明,硬壳蛤群体遗传相似系数最高(0.670 9),遗传多样性指数最低(0.236 0);菲律宾蛤仔群体遗传相似系数最低(0.592 5),遗传多样性指数最高(0.261 8);根据遗传相似系数采用UPGMA法构建了4物种32个体的聚类图,表明文蛤与菲律宾蛤仔遗传关系最近,青蛤与其他3物种遗传关系较远。     

经济海藻病害及防控技术的研究进展

经济海藻的病害一直是海藻人工栽培的一大障碍,往往造成严重的经济损失。目前对藻类病害的研究大多集中于病原鉴定,而病原的多样性和复杂的海区微环境增加了病原分离和鉴定的难度。本文综述了经济海藻病害发生的原因、病原的多样性和致病机制、病原的鉴定、检测和防治方法,并对未来我国海藻病害研究方向进行了展望,以期对藻类病害的防控提供参考。     

应用极化合成孔径雷达检测海上溢油研究进展

海上溢油给海洋生态环境带来严重的影响,快速准确地探测溢油对于防灾减灾具有重要的意义。利用卫星遥感探测溢油已成为目前主要的检测手段,大多采用合成孔径雷达(SAR)数据,运用图像处理的方法,开展了多种溢油提取算法的研究,取得了较好的结果,但由于海洋的类溢油现象存在,造成提取信息的精度达不到要求。近年来,国内外运用极化SAR数据开展溢油信息提取研究,从极化分解与相位差等角度对溢油特性分析,能有效地区分一些类溢油现象,得到了较理想的结果。分析了应用SAR数据开展溢油信息提取的研究状况,总结了溢油极化SAR探测的研究,指出了目前研究中存在的不足,并提出了今后溢油极化SAR遥感监测的方向。     

基于合成孔径雷达回波信号的海洋溢油监测方法研究

海洋油污染是各类海洋污染中最常见、分布面积最广且危害程度最大的污染之一。近年来,海洋特别是近海人类活动频繁,且随着海上运输和石油加工业的发展,油田井喷、钻井平台爆炸、船舶碰撞等所造成的溢油事故增多,因而,监测海洋溢油具有重要的经济和社会现实意义。研究采用MatLAB工具,通过图像预处理(图像校正和增强)、特征提取和神经网络识别等方法,对合成孔径雷达(SAR)海洋溢油图像进行处理,最终期望实现半自动区分SAR图像上各类目标,并进行多种神经网络方法效果比较。研究首先对SAR海洋溢油图像进行初步人工识别;然后进行图像预处理(几何校正、滤波处理等)和基于灰度共生矩阵的特征值计算;最后,借助神经网络方法对溢油区域和疑似溢油区域进行分类,输出分类处理后的图像。通过输出图像分析发现,神经网络能对SAR海洋溢油图像中溢油、海水、土地3类目标进行明确分类,且RBF神经网络模型精度高于BP神经网络。本文提出的半自动分类方法不仅能提高SAR图像处理效率,将分类目标扩充有溢油和非溢油扩充到溢油、海水、土地3类,提高图像处理的全面性,同时通过比较RBF和BP神经网络在SAR溢油图像分类上的具体优劣,有着较好实际意义。     

中国南海区1992-2012年的中尺度涡旋演化过程表达与统计分析

Automated identification and tracking of mesoscale ocean eddies has recently become one research hotspot in physical oceanography. Several methods have been developed and applied to survey the general kinetic and geometric characteristics of the ocean eddies in the South China Sea（SCS）. However, very few studies attempt to examine eddies＇ internal evolution processes. In this study, we reported a hybrid method to trace eddies＇ propagation in the SCS based on their internal structures, which are characterized by eddy centers, footprint borders, and composite borders. Eddy identification and tracking results were represented by a GIS-based spatiotemporal model. Information on instant states, dynamic evolution processes, and events of disappearance, reappearance, split, and mergence is stored in a GIS database. Results were validated by comparing against the ten Dongsha Cyclonic Eddies（DCEs） and the three long-lived anticyclonic eddies（ACEs） in the northern SCS, which were reported in previous literature. Our study confirmed the development of these eddies. Furthermore, we found more DCE-like and ACE-like eddies in these areas from 2005 to 2012 in our database. Spatial distribution analysis of disappearing, reappearing, splitting, and merging activities shows that eddies in the SCS tend to cluster to the northwest of Luzon Island, southwest of Luzon Strait, and around the marginal sea of Vietnam. Kuroshio intrusions and the complex sea floor topography in these areas are the possible factors that lead to these spatial clusters.     

南海北部陆缘合浦盆地烃源岩特征与低熟油气及生物气勘探前景

南海北部陆缘广西合浦盆地油气勘探迄今尚未取得突破,其根本原因是对该区基本油气地质条件及油气地质规律认识尚不够深入。合浦盆地主要烃源岩与百色盆地一样,均属成熟度偏低的始新统中深湖相暗色泥质岩系,其低熟烃源岩与低熟油气特征及其成藏地质条件亦基本一致。在深入分析研究合浦盆地低熟烃源岩有机质丰度、生源母质类型及热演化特征的基础上,重点对其低熟油气及生物气的地球化学特征与百色盆地进行了类比,指出合浦盆地古近系具备低熟油气/生物气的基本地质条件,其油气勘探方向及勘探策略,应借鉴百色盆地的成功经验,重点勘探寻找低熟油气及生物气资源。     

Lacustrine basin unconventional resource plays: Key differences

There has been a revival in hydrocarbon source rock characterization and development associated with growing interest in unconventional resources, where these fine-grained organic-rich rocks act as both source and reservoir. To-date, the exploration focus on shale reservoirs has been largely on marine systems. Lacustrine source rocks for conventional resources are geographically important, dominating regions such as China, Indonesia, and Brazil's resource-base. However, they have been generally untested for unconventional resources.There are a number of key differences in the nature of these hydrocarbon systems that should be considered when assessing whether lacustrine systems may represent future unconventional opportunities in areas where the conventional resource-base is dominated by lacustrine-sourced oil. Among the key differences between these depositional systems is the greater sensitivity to high frequency climatic variability within lacustrine systems. Lacustrine systems are highly sensitive to changes in the balance between precipitation and evaporation, which may lead to rapid changes in lake level, potentially exceeding 600 m. These changes in depositional conditions are geologically rapid and may occur over periods of thousands of years. Such changes can reduce the areal extent of potentially thick source rock intervals to only those portions of a basin where a permanent deep lake was present. Thus the core unconventional target area may be geographically limited compared with their marine counterpart. Although potentially areally limited, a review of many lacustrine source rocks suggests that their thicknesses are often significantly greater than marine source rocks. An examination of the more distal portions of lacustrine systems, where better source rock potential is present reveals that there is generally limited connectivity between source and conventional reservoir. In these settings, such as the Wind River basin (Waltman Shale), the hydrocarbons remain trapped within the shales, potentially leading to over-pressured hydrocarbon charged systems. Such conditions suggest that although areally limited, viable unconventional targets may exist, if suitable reservoir conditions are present. Finally, the character of the oils produced is different in these settings, with lacustrine oils being waxy and displaying different hydrocarbon generation and cracking kinetics. High wax oils display distinct flow characteristics, being more viscous, and may offer different production challenges than their non-waxy marine equivalents. Additionally, differences in their cracking kinetics may indicate that the timing of gas generation for shale gas plays may differ significantly from marine systems.     

基于资源等价分析法的海洋溢油生态损害评估模型及应用

采用资源等价分析法构建溢油海洋生态损害评估的模型,重新对2002年渤海塔斯曼溢油事件的潮间带生物损害进行定量评估,得出补偿修复工程的规模从而估算出针对潮间带受损资源的赔偿额度。资源等价分析法的研究与应用填补了国内在该类方法研究领域的空白,进一步完善了中国溢油生态损害评估方面的研究,为各类溢油损害计算提供参考和补偿依据。     

基于可靠性的海洋石油平台维修策略研究

针对海洋石油平台传统维修方式依赖经验的缺陷,从可靠性分析角度来制定维修策略,包括确定最佳维修周期和制定设备的检修顺序。以胜利油田埕岛中心一号海洋石油动力平台电力系统为例,采用基于最小割集的数值仿真方法进行故障树分析,通过任务可靠度推出任务时间,实现以可靠性为中心的最佳预防性维修周期的确定。此外,对系统进行重要度分析,按关键重要度大小进行排序,从而制定设备的检修顺序。     

海洋来源羽毛降解菌株的筛选、鉴定及酶学性质研究

微生物降解羽毛既可保护环境又能实现废物的资源化利用,具有显著的经济效益和社会效益。本研究从广西钦州港海鸭养殖淤泥筛选获得一株可高效降解羽毛的菌株Gxun-20,通过对该菌株形态特征、生理生化特性和16S rRNA基因序列进行分析,以确定菌株的分类,并对菌株产角蛋白酶的条件及酶学性质进行研究。结果表明：该菌为副短短芽孢杆菌(Brevibacillus parabrevis);菌株最适产酶条件为：温度34℃,初始pH 6.5,羽毛含量1.5%(质量分数),发酵48 h,角蛋白酶活性可达277.45 U/mL。菌株Gxun-20所分泌的角蛋白酶最适温度为50℃,且热稳定性较好,最适pH为7.5;金属离子Fe³⁺、Cu²⁺、Co²⁺、Fe²⁺和Mn²⁺对酶活性有显著抑制作用,而Ca²⁺、Na⁺对酶活性有明显促进作用;金属螯合剂乙二胺四乙酸(ethylene diamine tetraacetic acid, EDTA)可显著抑制酶的活性,蛋白...