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斑纹鲟凝集素(CFL)的初步研究Ⅱ.CFL的分子组成及性质鉴定 总被引:3,自引:0,他引:3
采用生化方法对斑纹鲟(Charybdis feriatus)凝集素进行了初步研究.结果表明,斑纹鲟的血清中有动物红细胞和微生物细胞凝集活性的凝集素(Charybdis feriatuslectin,CFL),其活力表现依赖于Ca2+,并为多种糖所抑制;CFL对40℃以上的温度敏感,其活力在pH6.0~8.0内稳定.经巯基乙醇处理和未经巯基乙醇处理的CFL在SDS-PAGE上均为一条蛋白带,说明斑纹鲟凝集素不含二硫键,且只有一种亚基,分子量为40.8 kDa,CFL氨基酸组成分析表明其富含酸性氨基酸. 相似文献
13.
东海陆架北部泥质区沉积动力过程的季节性变化 总被引:4,自引:0,他引:4
利用东海陆架北部泥质区(济州岛西南)及其周围不同季节5个航次的悬浮体资料和相应的底质粒度资料,对该泥质区沉积动力过程的季节性变化规律进行了分析。结果表明:东海陆架北部悬浮体的高含量区并不与该区的沉积中心(泥质区)相吻合,泥质区的形成需有物源的充沛供应和涡旋的动力效应相结合。泥质区沉积作用时空变化具有强烈的季节性。冬季泥质区悬浮体供应充足,涡旋中心向泥质区东南扩展,是本区接受悬浮体沉积的关键季节。夏、秋二季泥质区的大部分区域缺少悬浮体物源,涡旋中心向北退缩,致使泥质区沉积作用强度降低,并且主要影响泥质区的西北区域。春季泥质区沉积作用强度和影响范围大于夏、秋两季,但小于冬季。 相似文献
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Results are presented from a series of settling column experiments investigating temporal variations in the flocculation characteristics of purely cohesive (kaolin clay) sediment suspensions and cohesive (kaolin) and non-cohesive (fine sand) sediment fraction mixtures. Experimental runs were conducted under controlled hydrodynamic conditions generated by a rigid array of in-phase oscillating grids. The results indicated that rapid initial floc aggregation occurred under low turbulent shear rates, with peak maximal and root-mean-square (r.m.s.) floc sizes (∼ 400 μm and ∼ 200 μm, respectively) attained after relatively short time periods, before reducing with time. By contrast, lower aggregation rates and smaller floc sizes were observed under higher shear conditions, with flocs retaining suspended in the settling column for longer time scales due to the increased turbulence. The mud input concentration displayed some correlation with maximal and r.m.s. floc sizes at higher shear rates but no correlation was apparent at low shear rates. This observed floc behaviour may be attributed to the differences in concentration gradients at high and low shear rates that affect both floc settling rate and time required for flocs to attain equilibrium size. The addition of the fine sand fraction to the kaolin clay suspension reduced both the initial floc formation (i.e. aggregation) rate and the maximal and r.m.s. floc sizes attained throughout the experiments. The reduction in maximal floc sizes appeared to be enhanced by an increase in the ratio of fine sand to kaolin clay content within the mixture. 相似文献
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W. Lindsay Whitlow 《Marine Ecology》2010,31(3):418-430
Many studies on invasive species show reduced native densities, but few studies measure trait‐mediated effects as mechanisms for changes in native growth rates and population dynamics. Where native prey face invasive predators, mechanisms for phenotypic change include selective predation, or induced behavioral or morphological plasticity. Invasive green crabs, Carcinus maenas, have contributed to declines in native soft‐shell clams, Mya arenaria, in coastal New England, USA. We tested the hypothesis that clam ability to detect chemical cues from predators or damaged conspecifics would induce greater burrowing depth as a refuge from invasive crabs, and greater burrowing would require increased siphon growth. To determine how crab predation affected clam survivorship and phenotypic traits in the field, clams in exclosure, open, and crab enclosure plots were compared. Crab predation reduced clam density, and surviving clams were deeper and larger, with longer siphons. To determine whether the mechanism for these results was selective predation or induced plasticity, phenotypes were compared between clams exposed to chemical cues from crab predation and clams exposed to seawater in laboratory and field experiments. In response to crab predation cues, clams burrowed deeper, with longer siphons and greater siphon mass. Overall, crab predation removed clams with shorter siphons at shallow depths, and crab predation cues induced greater burrowing depths and longer siphons. Longer siphons and greater siphon mass of deeper clams suggests clams may allocate energy to siphon growth in response to crabs. By determining native behavior and morphological changes in response to an invasive predator, this study adds to our understanding of mechanisms for invasive impacts and illustrates the utility of measuring trait‐mediated effects to investigate predator–prey dynamics. 相似文献
17.
泥底辟/泥火山流体热效应对天然气水合物赋存的影响是探索泥底辟/泥火山发育区水合物赋存规律的关键问题。笔者在分析总结国外泥底辟/泥火山流体热异常及其对天然气水合物赋存之影响的基础上,重点探讨了我国南海北部陆坡珠江口盆地神狐海域SH5站位未钻获水合物的原因及泥底辟/泥火山流体热效应对天然气水合物赋存的影响。当初寄予厚望的位于泥底辟之上的SH5站位未成功钻遇水合物的原因是晚期泥底辟刺穿水合物稳定带使深部高温流体沿着泥底辟和断裂通道上侵,该过程致使水合物稳定带温度场升高进而导致早期形成的水合物发生分解,因此,泥底辟/泥火山的流体热效应会直接制约水合物的赋存。探索泥底辟/泥火山流体热效应及其对水合物成藏的影响,不仅为丰富和完善流体渗漏构造环境下天然气水合物成藏动力学研究与勘探提供了热力学理论依据,而且对天然气水合物勘探开发及资源潜力评价等具有重要的指导意义。 相似文献
18.
综述了近10a国内外有关重金属、氨态氮、亚硝酸盐、有机污染物等几种环境因子对虾蟹类非特异性免疫影响的研究成果,以期为通过改善养殖环境条件提高虾蟹类自身免疫抗病力提供理论依据.同时,为进一步深入开展该领域的研究工作积累资料. 相似文献
19.
虾蟹混养塘中WSSV对三疣梭子蟹(Portunus trituberculatus)致死效应的定量研究 总被引:1,自引:0,他引:1
采用实时荧光定量PCR法,在对白斑综合症多发的虾蟹混养塘内三疣梭子蟹体内病毒含量进行定量跟踪检测的基础上,结合电子显微镜及病理切片,对室内经口服、注射两种回感模式处理已感染白斑综合症病毒的濒死三疣梭子蟹体内病毒含量进行实验验证,并观察其体内主要器官组织的病理改变。结果表明,受白斑综合症病毒感染的三疣梭子蟹存活与死亡取决于蟹体内病毒含量,当病毒含量≥109拷贝/ml时均进入濒死或死亡阶段,其主要器官组织均有明显的病理改变。 相似文献
20.
A field experiment was carried out to test the effect of pore water salinity on the macrobenthic assemblages in an estuarine region of the Tagus estuary (Portugal) subjected to wide fluctuations in salinity. The conditions at the experimental site ranged from freshwater (minimum salinity 0.2) to mesohaline (maximum salinity 15.3). The experimental site was affected by an unexpected deposition of fluid mud during summer. Redundancy Analysis discriminated the experimental treatments along the first canonical ordination axis. The analysis also revealed an experimental gradient of increasing environmental stress, in which the minimal presence of organisms corresponded to treatments representing a high level of environmental stress. Sediment dynamics and saline fluctuations were the major factors that, together, determined the low macrofaunal abundance and species diversity at the experimental site. The most abundant macrofaunal species in this harsh environment were the polychaetes Hediste diversicolor and Streblospio shrubsolii. 相似文献