深圳华侨城湿地物种多样性及其保护研究

深圳华侨城湿地位于珠江口深圳湾北岸,通过箱涵与深圳湾海水相通,总面积69万m2,水域面积约50万m2,是深圳湾湿地生态系统的重要组成部分。对华侨城湿地植被、鸟类、底栖生物、浮游生物的多样性进行了调查,结果表明:华侨城湿地共有植物60科162种,其中真红树植物5科6属7种,半红树植物3科4属5种,外来入侵植物27种;鸟类共有12目36科101种,约占深圳湾鸟类总种数的50%,其中国家Ⅱ级重点保护野生鸟类6种;共鉴定出藻类5门11属14种;浮游动物25种。华侨城湿地面临严重的生态危机,主要包括入侵植物大面积分布、物种单一,鸟类、底栖生物类等生物多样性与福田红树林保护区及凤塘河口相比较低,并据此提出了相应的保护建议。     

莫莫格国家级自然保护区的鱼类多样性

2008～2010年,在鱼类资源调查的基础上,分析了莫莫格国家级自然保护区的鱼类多样性特征。调查结果显示,莫莫格国家级自然保护区有鱼类物种5目11科42属49种,其中本区土著种5目10科38属45种,包括中国特有种4种,中国易危种5种,冷水种8种,国家II级保护动物1种。土著种群落中,有鲤形目(Cyprini-formes)鱼类32种,鲤科(Cyprinidae)鱼类29种,鮈亚科(Gobioninae)鱼类13种;古北界北方区黑龙江亚区黑龙江分区的物种为22种;源于东部平原和北方平原生态类群的鱼类有27种;适应于江—湖缓流环境和淡水定居生活的鱼类有33种;草上产卵和水层产卵繁殖的鱼类有37种;杂食性和初级肉食性种类有28种,它们均相对占优势。莫莫格国家级自然保护区与嫩江的鱼类群落物种结构Jaccard系数和Morisita-Horn相似性指数分别为0.918和0.838;稀有种(包括中国易危种)和常见种分别为14种和25种;物种多度格局近似于对数正态分布。这些结果表明,莫莫格国家级自然保护区的鱼类多样性与嫩江密切相关,鱼类区系复杂,物种组成与生态类型多样;群落结构相对较稳定,多样性程度相对较高,但仍面临着稀有种(包括中国易危种)尚占有一定比例而导致群落结构脆弱的潜在威胁。保持嫩江鱼类群落结构的稳定,对维护和发展莫莫格国家级自然保护区的鱼类多样性具有特别重要的意义。     

Organic matter of the Mulhouse Basin, France: a synthesis

The lower Oligocene evaporite sequence of the Mulhouse Basin (France) contains organic matter-rich marl deposits. These marls display an overall cyclic variation of sedimentation rate, organic carbon content, hydrogen index and selected molecular parameters over a 30 m thick stratigraphic interval. The integration of all sedimentological and geochemical parameters has allowed the reconstruction and characterization of the paleoenvironment of deposition. The marls were deposited in a perennial lake that was at times connected to the sea. Two organic facies end members could be assigned to a lake stage with a marine connection and a lake stage that received dominantly continental water input. The overall stratigraphic variation in the organic matter content is interpreted to reflect the adaptation of the Oligocene flora to the changing paleoclimate and environmental conditions.     

Nematode distribution in cultivated and undisturbed soils of Guinea Savannah and Semi-deciduous Forest zones of Ghana

Climate change affects air temperature, sea levels as well as the soil and its ecosystem. The Guinea Savannah and Semi-deciduous Forest zones of Ghana are characterized by different climatic conditions and vegetative cover. Annual average temperature has been steadily increasing whilst annual total rainfall has been decreasing in both zones, and this has been causing a southward shift of the Savannah into the Forest zone. Soil organisms provide crucial ecosystem services which are required for sustainable agriculture and food production yet crop cultivation disturbs the soil ecosystem. The harsh conditions associated with the Savannah further expose the soil ecosystem to disturbance and loss of biodiversity which threatens food production and security. Soil nematodes are the most abundant animals in the soil and play a central and critical role in the soil food web complex. Studying the nematode community structure gives a reflection of the status of the entire soil ecosystem. Soil samples were taken from cultivated and natural landscapes in the Guinea Savannah and Semi-deciduous Forest agroecological zones to analyse the nematode community. Results from the study showed the Guinea Savannah zone recording warmer soil temperatures, lower organic matter percentage and lower nematode diversity(Genus Richness) as compared to the Semi-deciduous Forest zone. If the Savannah continues to shift southward, the Forest zone soil ecosystem risks disturbance and loss of biodiversity due to the harsh Savannah conditions. Our findings indicate that prevailing crop cultivation practices also disturb soil ecosystem in the two ecological zones which span across West Africa. A disturbed soil ecosystem endangers the future of food production and food security.     

地质体中主要生物分子的研究方法及应用

现代分子生物学,生物化学及有机地球化学等领域的发展,为古生物研究者在发子水平上探索地质生物世界提供了新的概念和技术手段,本文着重讨论了可用于分子古生物学研究的生物分子,例如,维管植物的木质素等稳定生物聚合物,古生物细胞内的类脂化合物,碳水化合物,蛋白质及氨基酸和含遗传信息的核酸,分子古生物资料可用于探讨化石埋藏学,古生物分子系统学,生物演化模式与机制,分子演化速率,古生态环境再造等问题,本文较详细     

翘嘴红鲌(Erythroculter ilishaeformis Bleeker)种群遗传多样性的RAPD分析

采用RAPD技术对翘嘴红鲌养殖群体进行了DNA多态性检测.共采用40个随机引物进行扩增,其中14个引物可获得清晰且重复性好的扩增图谱,共扩增出121个RAPD位点,具有多态现象的位点为40个,多态位点比例为33.06%.翘嘴红鲌群体的遗传相似系数为 0.8878-0.9600,平均为0.9201,遗传变异度为0.0799.Shannon多样性指数为10.8824,Shannon多样性值为0.0899.研究表明,翘嘴红鲌目前的种质资源状况令人堪忧,改变目前人工繁育模式,建立原种、良种场,丰富物种遗传多样性是资源保护的基础.     

On The Potential of Local Political Projects: A Response To Lake,Martin, and Wilson

Gentrification in the form of "neighborhood revitalization" is increasingly touted as one way of decreasing the social exclusion of residents of poor inner-city neighborhoods and of increasing levels of social mix and social interaction between different classes and ethnic groups. Yet the gentrification literature also suggests that the process may lead to increased social conflict, displacement of poorer residents to lower quality housing elsewhere, and, ultimately, social polarization. Much of this hinges on whether gentrifying neighborhoods can remain socially mixed, and whether neighborhood compositional changes result in more or less of a polarized class and ethnic structure. However, the impact of revitalization and gentrification on levels of social mix, income polarization, or ethnic diversity within neighborhoods remains unclear and under-explored. This study addresses this gap by examining the relationship between the timing of gentrification, changes in the income structure, and shifts in immigrant concentration and ethnic diversity, using census tract data for each decade from 1971 to 2001 in Toronto, Montreal, and Vancouver. This research demonstrates that gentrification is followed by declining, rather than improving, levels of social mix, ethnic diversity, and immigrant concentration within affected neighborhoods. At the same time, gentrification is implicated in the growth of neighborhood income polarization and inequality.     

半滑舌鳎(Cynoglossus semilaevis)双链RNA依赖的蛋白激酶R(PKR)基因克隆和实时定量表达分析

采用RACE方法克隆了半滑舌鳎(Cynoglossus semilaevis)PKR基因(CsPKR), 获得了CsPKR全长cDNA序列为2907bp, 其中包含1959bp的开放阅读框, 100bp的5′非编码区和848bp的3′非编码区。保守结构域分析显示推导的CsPKR氨基酸在N端存在两个特异的dsRBD(dsRNA binding domain dsRBD)结构域, 在C端具有多个保守的激酶活性位点, 包括ATP结合位点和底物配体结合位点, 以及活性环结构A-loop。系统进化树分析显示鱼类、两栖类、鸟类及哺乳类的PKR具有共同的进化起源, CsPKR与牙鲆PKR的亲缘关系最为密切。荧光定量PCR结果显示, CsPKR基因在健康鱼多个组织中广泛表达, 在脑中的表达最高, 头肾中表达最低。经鳗弧菌和淋巴囊肿病毒分别感染后, CsPKR基因在免疫相关组织中呈现上调表达趋势, 其中感染鳗弧菌12h后在血液中表达量较对照组上升了9.28倍, 在感染淋巴囊肿病毒24h后, 在肝脏中的表达达到最大, 为对照组的9.97倍。以上结果暗示CsPKR基因在半滑舌鳎响应细菌和病毒免疫应答中起重要作用。     

Species richness,diversity and density of understory vegetation along disturbance gradients in the Himalayan conifer forest

We investigated whether species richness, diversity and density of understory herbaceous plants differed along logging(gap) and grazing(primarily by cattle) disturbance gradients, and sought to identify drivers of richness, diversity and density of understory vegetation of logged sites. A factorial experiment was conducted in the mixed conifer forest of Gidakom in Western Bhutan. Levels of the logging treatment included small(0.15 – 0.24 ha), medium(0.25 – 0.35 ha) and large(0.36 – 1.31 ha) gaps. The grazing treatment included grazed(primarily by cattle) and ungrazed(where herbivores were excluded by a fence) plots nested within each gap. Data were collected from 12 gaps(4 replicates at each level of logging) using the point intercept method. Shannon Weaver Diversity and Margalef's indices were used to estimate species diversity and describe species richness, respectively. Soil samples were analyzed for pH and nutrients. The interaction effect of logging and grazing was significant(p≤0.001) only on species diversity. Relative to ungrazed areas, species diversity was significantly higher(0.01≤p≤0.05) in medium grazed gaps. Under grazed conditions, soil P was negatively correlated with gap size and species diversity. While species diversity was positivelycorrelated(0.01≤p≤0.05) with soil N in grazed plots species richness was positively correlated(0.001≤p≤0.01) with soil N in ungrazed plots. Relative density of Yushania microphylla and Carex nubigena were higher under ungrazed conditions. Our study suggests that the combined effect of cattle grazing and logging results in higher species diversity of understory vegetation in medium and grazed gaps in mixed conifer forests of Bhutan,whereas increase or decrease in relative density of major species is determined primarily by the independent effects of grazing and logging. From management perspective, forest managers must refrain from creating large gaps to avoid loss of nutrients(mainly P and N), which may eventually affect tree regeneration. Managers intending to maintain understory vegetation diversity must consider the combined effects of grazing and logging, ensuring low to moderate grazing pressure.     

Effects of low molecular weight organic acids on sorption and desorption behavior of p-chlorophenol by yellow earth

Low molecular weight organic acids （LMWOAs） are important components of root exudates. They play an important role in immobilizing and remobilizing contaminants in rhizospheric soil. Effects of four LMWOAs on the sorption and desorption behavior of p-chlorophenol by yellow earth was studied in batch mode. The results showed that the previous application of LMWOAs to enhanced adsorption capacity of p-chlorophenol of the soil in the order of maleic acid〉malic acid〉oxalic acid〉citric acid. However, when LMWOAs were applied to soil where p-chlorophenol had been previously adsorbed, substantial p-chlorophenol was desorbed from soil by oxalic acid, whereas citric acid, malic acid and maleic acid didn＇t desorb as much p-chlorophenol from soil as deionized water.