Major human Hepatitis A virus genotype in Hong Kong marine waters and detection by real-time PCR

Marine waters from seven sites around Hong Kong with varying levels of sewage pollution were analyzed for Hepatitis A virus (HAV) by PCR cloning and DNA sequencing of the highly variable VP1/2A junction of the HAV genome. Phylogenetic analysis of 10 PCR clones from each of the HAV-positive marine sites indicated that human HAV genotype IB is the most widely distributed type in Hong Kong waters. A sensitive and quantitative TaqMan-based PCR method targeting the 5′-noncoding region (5′-NCR) of HAV was used to quantify HAV particles in marine water samples along with the total Escherichiacoli counts being enumerated on TBX medium for comparison. Our results showed that no correlation of any significance between HAV and E. coli counts was observed which underscores the inadequacy in using E. coli as a sanitary standard to predict the levels of HAV in marine waters.     

岐口海洋地震观测台的建设

天津市建设项目"海上地震观测试验系统",是"十五"期间天津数字地震观测网络项目中的重点实验任务,该项目的建设将有效提高渤海海域地震监测能力,为天津经济社会发展建设、为滨海新区开发开放提供服务.通过建设海洋地震台,提高了天津市测震台网在渤海地区的监控能力,对于海啸预警能力也大有提高.     

Geochemical Stratigraphy and Microvertebrate Assemblage Sequences across the Silurian/Devonian Transition in South China

Carbon isotope (δ13Corg) analyses of non-marine clastic rocks and neritic carbonates and black shales spanning the Silurian/Devonian transition are compared from two richly fossiliferous sequences in Qujing of East Yunnan and Zoige of Sichuan, South China. The two sections, Xishancun and Putonggou sections in South China, reveal positive δ13Corg shifts happening in the Upper Pridoli and Lower Devonian and reaching peak values as heavy as ?25.2‰ (Xishancun) and ?19.9‰ (Putonggou) in the lowermost Lochkovian following the first occurrence of the thelodont Parathelodus and the conodont Icriodus woschmidti woschmidti (only in Putonggou Section and together with Protathyris-Lanceomyonia brachiopod fauna). These results replicate a globally known positive shift in δ13Corg from the uppermost Silurian to the lowermost Devonian. The δ13Corg variations across the Silurian/Devonian Boundary (SDB) at the two sections in South China exhibit a shift in carbon isotopic composition similar to the detailed SDB curves from the borehole Klonk-1 drilled at top of the Klonk Global Standard Stratotype-Section and Point (GSSP) in the Prague Basin, Czech Republic. In addition, four microvertebrate assemblages, including the Liaojiaoshan, Xishancun, Yanglugou and Xiaputonggou assemblages, are recognized from the Silurian/Devonian transition exposed in the Xishancun and Putonggou sections, respectively. The results from both carbon isotope stratigraphy and microvertebrate assemblage sequences suggest that the SDB in South China is located at the base of the Xishancun Formation (between sample QX-20 and sample QX-21) in the Xishancun Section and the lower part of the Xiaputonggou Formation (between sample ZP-09 and sample ZP-10) in the Putonggou Section. The isotopic trend for organic carbon together with the changes of microvertebrate remains across the SDB can offer an approach to a potential correlation of the SDB from different sedimentary facies, which help to correlate the marine with non-marine deposits.     

CMIP3模式对未来50a欧亚大陆雪水当量的预估

为研究预估未来50a欧亚大陆雪水当量,基于遥感数据,用误差百分率、空间相关和误差标准差等统计方法,评估了14个CMIP3模式在20C3M的雪水当量产品,诊断各模式对欧亚大陆雪水当量的模拟能力,在此基础上对模拟效果较好的10个模式产品进行多模式集合,分析了A2和B1情景下2002—2060年欧亚大陆雪水当量的变化．结果表...     

基于MODIS双卫星积雪遥感数据的积雪日数空间分布研究

结合Terra和Aqua卫星的积雪产品,获取2001-2006年全国新的逐日积雪覆盖数据,并利用此数据通过两种方案获取了全国积雪日数分布,对比发现3大稳定积雪区中,新疆地区积雪稳定性及连续性最好,东北其次,而青藏高原地区最差;通过595个气象台站年积雪日数数据分区分不同植被类型修正MODIS获得的年积雪日数.结果表明:...     

雪粒径遥感反演研究进展

介绍了雪粒径遥感反演的研究历史,阐述了球形或非球形假设下基于雪在可见光波段高反射,在红外区雪面反射率显著下降且对雪粒径变化敏感原理的遥感反演基础,其中包括诸如温度、波长、颗粒形状等,并总结了目前研究中所应用的模型和算法,指出其优点和不足之处.此外,为了得到反演粒径的准确性,讨论了诸如温度、波长、颗粒形状等因素对粒径反演...     

川东北地区海相烃源岩中单质硫的定性与定量分析

利用定性与定量分析手段,研究了川东北地区各类海相烃源岩中的单质硫及其地球化学特征。定性分析表明,该区海相烃源岩中的单质硫具有八硫环状结构和六硫状环结构两种晶体构型,由S8和S6两种同素异形体组成,其中S8具有相对较高的丰度。定量分析表明,该区海相烃源岩中的单质硫含量为0.91×10-6～375.88×10-6,具有很宽的分布范围,并且随着烃源岩分布区域、地层时代以及岩性的不同而发生明显变化,表现出强烈的非均质性分布特征。该区海相烃源岩中单质硫含量与有机碳含量之间存在正相关关系,说明单质硫主要发育在富有机质沉积的层段中。通过单质硫含量与总硫含量对比分析,间接证明了川东北地区各类海相烃源岩中赋存有丰富的有机含硫化合物。     

Variability of atmospheric freezing level height derived from radiosonde data in China during 1958–2005 and its impact to cryosphere changes

Atmospheric air temperature data from 92 stations in China’s radiosonde network were used to analyze changes in the freezing level height (FLH), glacier snow line, and ice edge from 1958–2005 (48 years) and to examine the impact of these changes on the cryosphere. In general, the FLH, glacier snow line, and ice edge exhibited latitudinal zonation, declining from south to north. Trends in the FLH, glacier snow line, and ice edge showed spatial heterogeneity during the study period, with prevailing upward trends. Temporally, the FLH, glacier snow line, and ice edge trends differed on various time scales.     

Dark respiration in the light and in darkness of three marine macroalgal species grown under ambient and elevated CO2 concentrations

Dark respiration (non-photorespiratory mitochondrial respiration),which occurs both in the light and in darkness,is vital for growth and survival of algae and plays a critical role in modulating the carbon balance of them.In the present study,we have investigated dark respiration in the light (R L) and in darkness (R D) in three marine macroalgal species,Hizikia fusiformis (phaeophyta),Gracilaria lemaneiformis (Rhodophyta) and Ulva lactuca (Chlorophyta),cultured at 20℃ using aeration with two CO 2 conditions:current ambient (CO 2 concentration about 380 μl/L) and elevated CO 2 (approximately 720 μl/L) air.R L was estimated by using the Kok method,whereas R D was determined as the rate of O 2 influx at zero light.The results showed that both R L and R D were unchanged for the elevated CO 2-grown algae relative to ambient CO 2 concentration for all the algal species tested.However,R L was significantly lower than R D across all the algal species and growth CO 2 treatments,demonstrating that daytime respiration was partly depressed by the light.The percentage of inhibition of respiration by light was similar between ambient and elevated CO 2 grown algae.The ratio of respiration to photosynthesis,which tended to decrease when estimated using R L instead of R D,was not altered for the elevated relative to ambient CO 2 concentration.The results suggest that R L,rather than R D,is a more accurate estimate of nonphotorespiratory carbon loss in marine macroalgae during the daytime.It would not be anticipated that elevated atmospheric CO 2 would exert a substantial influence on respiratory flux either in the light or in darkness in these particular marine macroalgal species.     

海洋微生物偏振光散射的双组分模型

光学散射方法被广泛用于获取海洋微生物的形态和浓度信息.通过测量散射光的空间分布可以测量海洋微生物的形态和浓度;而最近发现,散射光的偏振特征却对细胞内部亚微米级散射颗粒更加敏感.把海洋微生物等效为粒径远大于入射波长的“米氏”粒子和远小于入射波长的“瑞利”粒子的混合体,以两种不同粒径的实心球作为微生物偏振光散射模型.利用蒙...