基于形态学与核糖体DNA及其cDNA高通量测序的海洋沉积物中纤毛虫多样性比较

高通量测序技术广泛应用于环境真核微生物多样性的研究,可检获较之传统形态学方法更高的多样性。然而,检获的高分子多样性与基于形态的物种多样性在构成上的差异仍然不明。本研究首次对比分析了基于形态学的南黄海沉积物中的纤毛虫物种多样性与基于核糖体18S DNA和c DNA高通量测序的多样性异同。结果表明:形态鉴定获得8纲、20目、30科、36属共97种纤毛虫;DNA测序检获10纲、28目、55科、76属共174个OTUs;而通过c DNA测序获取的纤毛虫多样性最高,获10纲、31目、68科、99属共284个OTUs。研究发现,形态学方法检获的纤毛虫均为底栖生纤毛虫;两种分子手段检获的多样性更高,群落结构更为近似,覆盖了形态鉴定所获的绝大部分类群。但DNA测序还检获了序列比例高达90%的浮游类群,可能源于包囊或死亡沉降的物种;而c DNA测序检获了约7%的浮游纤毛虫序列。较之DNA测序,c DNA法检获的底栖纤毛虫在群落结构上与形态学结果更为接近。本研究表明,分子手段有助于更全面地揭示沉积物中的纤毛虫多样性,DNA测序可同时揭示休眠包囊、胞外DNA及过去群落的信息,而c DNA测序在研究活动纤毛虫多样性上更有优势。     

Spatio‐temporal variation in density and size structure of the endangered sea cucumber Isostichopus fuscus in Huatulco National Park,Mexico

The brown sea cucumber Isostichopus fuscus (Ludwig, 1875) has been subjected to strong fishing pressure and is currently considered an endangered species by the IUCN Red List of Threatened Species. Spatial and temporal variations in density were analysed and population parameters of I. fuscus were estimated in three localities of the Southern Mexican Pacific. Density was estimated using circular transects covering a 300 m² area in each locality. Body length and weight were also measured. The mean density was 0.018 ± 0.013 individuals·m^?2, and was statistically significantly lower during summer. The mean length and weight were 22.4 ± 3.8 cm and 396 ± 130 g, respectively, and statistically significant differences were found among localities. The weight–length relationship was W = 0.28·L^1.32 and the mean relative condition index (Kn) = 1.04 ± 0.31. The von Bertalanffy growth equation was L_t = 38.7·(1–exp^(?0.4·t)). The lowest values of length, weight and age were found at Dos Hermanas, suggesting that this locality could be favorable for the settlement of larvae and recruitment and growth of juveniles. The present study is the second to estimate densities of I. fuscus and the first one to publish data on size structure and growth for the population at Bahias de Huatulco. The densities were so low in the three sampled localities that none of them would be able to support a fishery, even under a sustainable management scheme. The information collected in the present study could be helpful for management programs of the Huatulco National Park, as all the data used for these currently come mainly from the populations of I. fuscus in the Gulf of California.     

南黄海沉积物中活性铁氧化物对有机碳的保存作用

土壤和沉积物中活性铁对有机质的吸附对有机质具有长期稳定和保存作用,从而在地质时间尺度上缓冲大气CO₂浓度。本文利用连二亚硫酸钠还原性溶解提取活性铁氧化物（FeR）及与之结合的有机碳（Fe-OC）,定量研究了南黄海沉积物中FeR与OC之间的结合方式以及FeR对OC的保存作用,讨论了深度增加对二者相互作用的影响。结果表明,南黄海沉积物中Fe-OC占沉积物总有机碳的份数（f_Fe-OC）为（13.2±7.47）%,即活性铁对OC的年吸附量为0.72 Mt,占全球边缘海沉积物TOC年埋藏通量的0.44%。Fe-OC的平均OC:Fe为4.50±2.61,表明共沉淀作用对有机质的保存起重要作用,且其比值随海源有机质含量增加而增加。Fe-OC稳定碳同位素（δ13C_Fe-OC）结果表明,FeR优先保存活性有机质,但这种选择性随OC:Fe增大而减弱。随深度增加,f_Fe-OC和δ13C_Fe-OC均未表现出显著变化,这与该海域沉积物中有机质活性较低、铁还原作用较弱有关。     

一种基于16S rRNA基因检测海洋浮游细菌群落的微阵列基因芯片

A better understanding of bacterioplankton community shifts following change in marine environments is critical to predict the marine ecosystem function. In order to get a snapshot of the microbial taxonomy profiling of a wide range marine area, a quick, convenient and low cost method would be favorable. In this study, we developed a 16S rRNA gene-based microarray using ARB software, which contained 447 probes targeting 160 families of marine bacteria. The specificity, sensitivity and quantitative capability of this microarray were assessed by single cloned16S rRNA genes. The reliability of this microarray was tested by eight environmental samples. The results showed that the microarray was specific, only 1.16% false results were detected in five single-clone hybridization tests. The microarray could detect DNA samples as few as 1 ng/μL and the signal intensity could reflect the relative abundance of the bacteria in the range of 1 ng/μL to 100 ng/μL of DNA concentration. Hybridization with environmental samples showed that it can discriminate bacterioplankton communities by sites and time. High throughput sequencing results from the eight samples confirmed the hybridization results. It indicated that this developed microarray could be used as a convenient tool to monitor the bacterioplankton community in marine environment.     

海上溢油量评估方法研究综述

近年来,国内外海上溢油污染事故的频发,使得全社会对海洋污染的关注不断升温。海上溢油量是评价海上溢油事故威胁程度和确定溢油事故等级的重要指标,也是污染赔偿追责的重要依据,同时对于现场溢油应急处置和科学决策也有重要作用。本文总结分析了目前主要的4种海上溢油量初步评估方法,包括质量平衡法、工艺流程法、现场观测法和数值模拟法。不同溢油量估算方法适用的溢油源和溢油方式各有不同,估算的溢油量也不尽相同。实际应用中,通常根据具体情况选择多种方法进行溢油量综合评估。本文从实际应用出发,探讨几种可用的溢油量评估方法,并结合案例进行说明。     

Spatial and temporal variation in subtidal molluscan diversity amongst temperate estuarine habitats

Effective management of marine ecosystems is enhanced when detailed information on biodiversity is available. Key information to underpin management actions and conservation planning includes relationships between species assemblages and environmental gradients, and information on species distributions. We conducted a subtidal biodiversity assessment of surface‐dwelling subtidal molluscs in eight a priori defined habitat types using underwater visual censuses to quantitatively explore relationships between molluscan assemblages, and their correlation with benthic habitats and abiotic variables. In addition, variations in diversity were examined for two key habitat types (areas dominated by Dendronephthya australis and by filter feeders) over a period of 15 months to examine temporal change. We found that molluscs form distinct assemblages within subtidal habitats, but that assemblages within key habitats show inherent temporal variability. Regional (gamma) diversity of molluscs was found to result from a combination of: (i) within habitat alpha diversity, which increased with habitat complexity; (ii) between habitat beta diversity, with significant differences in molluscan assemblages amongst habitats with differing benthic growth, substrate type, and depth; and (iii) temporal beta diversity, with significant changes detected in molluscan assemblages over time. The results demonstrate how habitats and abiotic variables (principally depth and substrate type) combine to contribute to molluscan biodiversity in temperate estuaries, and illustrate the value of these factors as surrogates for surface‐dwelling subtidal molluscs in conservation planning.     

海底管道出露悬空环境风险的定量评价研究

海底管道是海洋油气输送的主要方式,其运行状况直接关系到海上油气田的安全和海洋生态环境的质量。本文先以海底管道出露悬空作为故障树的顶事件,分析出海洋环境中各项基本事件,构建针对海洋环境的海底管道故障树模型;再以灰色模糊识别为理论基础,量化对海底管道安全有影响的海洋环境因素,并以层次分析法对每个风险因素进行分级赋权,确定评价指标范围和权重,进而构建海洋环境条件的海底管道风险评价体系。利用该体系对平湖油气田海底管道4个区段进行环境风险评价,避免了将整段管道一同评价导致的信息均等化,评价结果不能真实反映风险等级高的区段的情况;评价结果与管道2014~2016年现场检测数据对比,两者均显示P2分区中海底管道的风险等级较高,说明了该模型具有一定的合理性和实用性。     

高压密闭消解-电感耦合等离子体光谱和质谱联合测定海洋地质样品中52种元素

An improved analytical method to determine the content of 52 major, minor and trace elements in marine geological samples, using a HF-HCl-HNO_3 acid system with a high-pressure closed digestion method(HPCD), is studied by an inductively coupled plasma optical emission spectrometry(ICP-OES) and an inductively coupled plasma mass spectrometry(ICP-MS). The operating parameters of the instruments are optimized, and the optimal analytical parameters are determined. The influences of optical spectrum and mass spectrum interferences, digestion methods and acid systems on the analytical results are investigated. The optimal spectral lines and isotopes are chosen, and internal standard element of rhodium is selected to compensate for matrix effects and analytical signals drifting. Compared with the methods of an electric heating plate digestion and a microwave digestion, a high-pressure closed digestion method is optimized with less acid, complete digestion,less damage for digestion process. The marine geological samples are dissolved completely by a HF-HCl-HNO_3 system, the relative error(RE) for the analytical results are all less than 6.0%. The method detection limits are 2–40μg/g by the ICP-OES, and 6–80 ng/g by ICP-MS. The methods are used to determine the marine sediment reference materials(GBW07309, GBW07311, GBW07313), rock reference materials(GBW07103, GBW07104,GBW07105), and cobalt-rich crust reference materials(GBW07337, GBW07338, GBW07339), the obtained analytical results are in agreement with the certified values, and both of the relative standard deviation(RSD) and the relative error(RE) are less than 6.0%. The analytical method meets the requirements for determining 52 elements contents of bulk marine geological samples.     

Management priorities for seawater desalination plants in a marine protected area: A multi-criteria analysis

The development of seawater desalination plants to increase water reliability in coastal areas poses a threat to the health of near shore marine ecosystems and may affect the effectiveness of marine protected areas (MPAs) that have been established to meet international conservation targets. This paper applies a multi-criteria analysis approach to quantify stakeholder groups’ priorities for seawater desalination plants that have been proposed in communities adjacent to a National Marine Sanctuary. All groups placed the highest importance on minimizing environmental impacts on protected areas and endangered species that could be affected by water intake and brine discharge emphasizing the need for integrated land and sea conservation. Minimizing socio-economic impacts on coastal communities was much less important. Stakeholders also weighted reducing pressure on water levels in rivers, streams, and aquifers as more important than increasing water for residential consumption, which may foster coastal growth rather than replacing water taken from other sources. The study further revealed differences in the importance of multiple management objectives among stakeholder groups, which highlights the need to elicit distinct priorities of all groups to understand concerns and potential conflicts of desalination with existing marine users. The analysis of consistency ratios revealed that around half of all surveyed stakeholders had high inconsistencies in their responses, which suggests either a lack of understanding of desalination, or reflects the complexity of establishing desalination plants in coastal areas adjacent to a marine protected area.     

南黄海西部2007年4月的逆温跃层

利用南黄海西部2007-04的温盐实测资料,采用海洋层结谱表达法及自适应识别,得到逆温跃层的"五点三要素",形成强度要素平面分布图.分析表明,逆温跃层的存在与黄海暖流水有直接的关系:1)4月份,黄海暖流水受到的海面冷却仍是产生逆温跃层的普遍原因,在该海区黄海暖流向北延伸和向两侧拓展的区域都有该种类型的逆温跃层存在,位置相对较浅;2)但在偏南的黄海暖流主干区,海面冷却产生的效应被主流区的热量补充所抵消,逆温跃层很弱甚至消失,这是该月份逆温跃层分布区向北退缩并在南部中心附近呈现缺失区的主要原因;3)南下的鲁北沿岸流水的冷水叠加在黄海暖流水的暖水上方,使逆温跃层加强,使得冷暖水的作用区成为强逆温跃层区;4)黄海暖流左侧冷沿岸流水及右侧冷水的前端向黄海暖流楔入,其前端往往覆盖在底层高温高盐的黄海暖流水上方形成下逆温跃层,从而形成双逆温跃层.这些特点,较以前认知更加客观、全面、细致和准确.