本溪县汤池沟热田热储特征及资源量评价

汤池沟热田大地构造位置处于辽阳－本溪凹陷与凤城凸起的交接部位,属浅埋藏水热对流型热田。热储由中生代燕山期花岗斑岩、凝灰岩组成。热源为岩浆活动的余热。热流体主要靠侧向基岩裂隙水及断裂充水带的水补给,自北东向南西方向以脉状裂隙水运动为主,热流体直接受北东、北北东及北西向断裂控制。该热田储存有较丰富的地热资源,热能总量计算为 58.05 TJ,目前的热水开采量每年为 414 000 t,相当于消耗燃烧值为 29.3 MJ的标准煤每年 1 833.43 t,如果按此潜力开发,其开采年限预计可持续 80年以上。     

Acute toxicities of potassium permanganate, formalin, and Lugol’s iodine solution to a marine ciliate, Pleuronema coronatum (ciliophora, scuticociliatida)

Acute toxicities of potassium permanganate, formalin, and Lugol’s iodine solution to a commonly occurred marine ciliate Pleuronema coronatum (Ciliophora, Scuticociliatida) were measured. Linear regression analysis of the results highlighted the close relationships between doses of the medicines and mortalities of the organisms, thus providing a capability to predict toxicity effects from the dose. Toxic effects of the medicines on the ciliates were described in the present paper, and the median lethal concentrations (LC₅₀ values) were given. Results of measurements indicated that 2 h-LC₅₀ and 12 h-LC₅₀ values of formalin on P. coronatum were 59.00×10⁻⁶ and 43.57×10⁻⁶, while those of Lugol’s solutions were 90.13 and 67.84×10⁻⁶ respectively. The tolerance of P. coronatum to formalin is apparently lower than that to Lugol’s iodine solution and potassium permanganate is a suitable medicine to kill ciliates in short time.     

Impact of Phenanthrene on Denitrification Activity and Transcription of Related Functional Genes in Estuarine and Marine Sediments

The effects of phenanthrene(Phe)on the denitrification activity and denitrifying genes(narG,nirS and nosZ)were evaluated by dose-response experiments in sediments of Dagu River Estuary(DRE)and Jiaozhou Bay(JZB).The results showed that potential denitrification activity(PDA),N2O,NO3−and NO2−reduction rates of both areas were inhibited with an increase of Phe concentrations.The PDA,N2O,NO3−and NO2−reduction rates of both areas was highest and lowest in the control(DRE:0.453,0.427,7.439 and 3.222mgNkg−1 h−1,JZB:0.592,0.555,8.470 and 3.793mgNkg−1 h−1)and highest Phe amended treatments(DRE:0.069,0.001,4.486,and 1.563 mgNkg−1 h−1;JZB:0.114,0.024,5.527 and 2.200 mgNkg−1 h−1).The inhibition rate of PDA was highest,follow by NO2−reduction and then NO3−reduction.Moreover,with the increasing of Phe concentrations,total bacteria count and the abundance of denitrifying genes were decreased.And N2O accumulation was promoted with the addition of Phe for both areas.Based on the comparison of EC50 values,denitrifiers harboring three genes were more sensitive to Phe than PDA,and denitrifiers harboring nirS gene were more sensitive,followed by nosZ gene,and then narG gene.Furthermore,according to correlation analysis,the relative abundance of denitrifying genes was much more positively correlated with PDA,NO3−and NO2−reduction than total bacteria count.In addition,the denitrification activity and total bacteria count in JZB were more inhibited than that of DRE.This study is useful for understanding the impact of Phe pollution on denitrification in estuary and marine sediments,with profound implications for the management of aquatic ecosystems regarding eutrophication(N-removal)and greenhouse effect.     

Method‐dependent influence of environmental variables on reef fish assemblages when comparing trap and video surveys

Despite substantial survey effort and a large body of literature on abiotic and biotic factors in temperate reef ecosystems, knowledge of the complex and interactive effects of environmental variables on those communities is limited. Various survey methods have been developed to study environmental predictors of biodiversity, but there remains a gap in our understanding of how survey results are influenced by environmental factors. Here, we surveyed the fish assemblage associated with southeastern U.S. temperate marine reefs with simultaneous, paired trap, and camera gears throughout a ~50,000 km² area during 2011–2013 and assessed the influence of environmental variables on the trap‐ and video‐surveyed assemblages. Predictor variables in the multivariate general linear models included depth, temperature, month, year, location, substrate relief, percent sessile biota, biota type, and turbidity. Depth and latitude had the greatest influence on the fish assemblage for both gears. The influence of habitat variables differed between methods and percent biota explained more variation in the fish assemblage when assessed by traps, while substrate relief and biota type explained more variation in the fish assemblage when assessed by video. In general, habitat complexity was positively related to the abundance of fishes in the video survey, but there was a negative relationship in the trap survey. Differences between gears were species‐specific and the influences of environmental variables were similar for some species such as Haemulon plumierii and Hyporthodus niveatus. The methods presented here can be used to assess method‐dependent differences in fish assemblages, which is a necessary precursor to assess the effect of environmental variables on the accuracy of surveys.     

莆田近岸海域海带烂孔病起因的初步研究

2019年3月莆田近岸海域海带尾部烂孔后逐渐向根部蔓延,孔洞相连后海带脱落,时空规律为先南后北逐渐发病,共涉及养殖面积约7 km2,给莆田近岸海带养殖带来了较大的损失。初步研究分析了海带大规模烂孔的原因,主要结果如下:海区温度异常升高,盐度持续较低,连续阴天光照不足,环境变化超出了细胞生理承受能力从而造成海带烂孔。此次诊断,对渔业生产及海洋环境监测结果在渔业上的应用均具有一定的现实意义。     

Polyclonal antibodies to light-harvesting CHL-protein of PSII (LHC II) in marine green algaeBryopsis corticulans

Polyc·lonal antibodies raised against LHC II isolated from SDS-solubilizedBryopsis corticulans thylakiod membranes by SDS-PAGE, were characterised by double immunodiffusion, Rocket immunoelectrophoresis and antigen-antibody crossed immunoelectro-phoresis assays showed the antibodies had strong cross-reaction with allB. corticulans LHC II components (even with those which were incubated in boiling water) and showed immunological cross-reactivity with LHC II polypeptides of spinach and the marine green algaCodium fragile. The results suggested that LHC II of different species had similar antigenic determinants and also conservation of amino acid sequences of the polypeptides during evolution, and that the antibodies could cross react with apoproteins of D₂ proteins (which contain P₆₈₀) fromB. corticulans, spinach andC. fragile, but not with apoproteins of P₇₀₀ Chl-proteins. Our results indicated some similarities in primary structure between LHC II of different species, and between LHC II and D₂ proteins of marine green algae and spinach. Our finding that D₂ and P₇₀₀ Chl-proteins are not immunologically related suggested that P₇₀₀ Chl-proteins and D₂ proteins pass through independent evolutionary pathways. Contribution No 1717 from the Institute of Oceanology, Academia Sinica, This subject was supported by the National Science Foundation of China.     

陕西省耕地资源数量与经济发展关系的计量分析

基于1978-2005年陕西省耕地资源数量与经济发展的统计数据,运用协整分析、误差修正模型、脉冲响应函数和方差分解等方法分析了陕西省耕地资源数量与经济发展之间的互动关系,结果显示:1)陕西省耕地资源数量与经济发展之间存在长期均衡关系,但短期内却存在失衡,短期波动向长期均衡趋近的调整幅度为4.23%,调整力度非常小;2)经济发展的冲击可以部分解释耕地资源数量的变化,解释水平为12%以下,耕地资源数量的冲击对经济发展的解释水平在18%以下;3)耕地资源数量、经济发展均显著地受其自身波动的影响.建议从提升经济发展质量和耕地利用效率等方面采取长期性、综合性的政府干预措施.     

光照强度对海洋微藻脂肪含量及脂肪酸组成影响的研究

近年来，海洋微藻脂肪酸组成的研究及其应用越来越受到国内外科学家的重视。高度不饱和脂肪酸(PUFA)，特别是长链的n-3 PUFA，如二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)对海洋动物和人类都具有营养学和医学上的价值。在海洋微藻的培养过程中，光照强度是海洋微藻生长的条件之一，光线的明与暗，光度的强与弱，不仅对微藻的生长速率、产量有影响，而且对其脂肪含量和脂肪酸的组成也有影响(Teshima et al.，1983；Thompson et al.，1990； Renaud et al., 1991)。 作者在以往研究的基础上，选择了3种有代表性的海洋微藻：(1)小球藻Chlorella sp-2(李荷芳等，1999)，此藻脂肪酸中EPA含量高、且不含DHA；(2)球等鞭金藻(Isochrysis galbana)，该藻DHA含量较高，但几乎不含EPA；(3)前沟藻(Amphidinium sp.)的EPA、DHA含量均高。将以上3种微藻作为原料，在不同的光照强度下进行培养，测定并分析藻体中的脂肪含量和脂肪酸组成的变化，从而了解光强对海洋微藻脂肪含量及其脂肪酸组成的影响。     

航海雷达溢油监测的同频干扰降噪处理方法研究

首先提出了在平面坐标下进行航海雷达同频干扰噪声处理;再采用卷积运算方法和Otsu算法分割航海雷达原始数据中的同频干扰噪声信号;最后应用横向均值运算方法处理同频干扰噪声。这种处理方法在对同频干扰进行降噪处理的同时,保留了含有溢油信息的海浪杂波信号,为航海雷达溢油监测技术的研究提供了更为准确的基础数据。