海洋浮游细菌生长率和被摄食的研究综述

海洋浮游细菌利用海水中的溶解有机碳合成自身物质,是海洋浮游生态系统的二次生产者。微型浮游动物是细菌的主要摄食者,也是细菌生产向较高营养级传递的中介。研究海洋浮游细菌的生长率和被(微型浮游动物的)摄食率对理解海洋浮游生态系统的功能具有重要作用。本文综述了利用改变海水中生物类群组成(或功能)的培养方法研究海洋浮游细菌生长率和被摄食率的历程和现状,为我国的同类研究提供借鉴。改变海水中生物类群组成(或功能)进行培养的方法有海水分粒级培养、海水稀释培养和添加选择性抑制剂培养。这些方法各有其局限性,应用并不广泛。细菌及其主要摄食者异养鞭毛虫群落在自然海区和实验室内都有生长周期,鞭毛虫的生长周期落后于细菌,因此细菌的生长率有时会小于被摄食率,有时会大于被摄食率。我国这方面的研究相对落后,应值得引起重视,建议从海水稀释培养法入手开展相关研究。     

区域海洋减灾能力评估指标体系构建与权重量化

为有效开展防灾减灾,需要充分了解区域海洋减灾能力现状,从而科学确定未来减灾能力建设的优先领域及重点工程。首先梳理了能力评估与灾害管理阶段、能力建设优先领域、能力评估对象与建设主体等要素之间的关系,以及评估指标体系共性设计与区域个性的关系;其次,通过组织部门及地方的相关减灾机构与专家开展多方参与式讨论,综合了海洋、水文、气象、民政、应急、教育及综合减灾等多个领域专家的减灾经验,构建了区域海洋减灾能力评估三级指标体系,包括6个一级指标、21个二级指标及136个三级指标;然后,基于专家问卷(有效问卷69份)及层次分析(AHP)方法,量化了三级指标体系中各指标的权重。区域海洋减灾能力评估三级指标体系及指标权重成果已在相关市县开展了评估试点应用,为未来在全国范围开展区域海洋减灾能力评估奠定了良好基础。     

海洋CSEM法探测二维海底天然气水合物响应特征

针对海底天然气水合物开展了可控源电磁探测法(Controlled-source Electromagnetic Method,CSEM)响应研究,根据实际地质资料设计二维地电模型并进行正演计算,讨论了频率变化对CSEM响应结果的影响以及海底地形起伏时的CSEM响应特征。综合天然气水合物二维正演模拟结果,论证了海洋CSEM探测海底天然气水合物的可行性,研究成果可为今后的海底天然气水合物勘探提供技术支持。     

现代海洋牧场建设之我见

现代海洋牧场建设是实现我国海洋渔业与近海生态系统和谐发展的重要途径之一,作为一种海洋经济新业态,海洋牧场既能养护渔业资源,又能修复生态环境。本文论述了现代海洋牧场建设的必要性,分析了我国海洋牧场建设中存在的建设技术缺乏标准化、承载力评估缺乏模型化、建设理念缺乏生态化、管理缺乏信息化等问题,探讨了现代海洋牧场相关理念及分类模式。针对现代海洋牧场建设存在的突出问题,提出了构建陆海统筹现代海洋牧场亟待解决的技术先导和"四化"突破(工程化、机械化、自动化和信息化)等对策和建议。     

Accurate Acoustic Signal Parameter Estimation for Marine Geodesy Surveys

Acoustic signal parameter estimation is important for diverse marine geodesy surveys and several other applications. However, the received signal from a far-field target characterized by planar wavefront propagation is frequently affected by strong nearby interfering signals. Their presence deteriorates the performance of direction-of-arrival (DOA) estimation for far-field target. In order to enhance the reception of signal from far-field target, the near-field/far-field (NFFF) beamformer is proposed. Such a beamformer optimizes beam pattern for far-field detection by maximizing beamformer output in the direction of the far-field target with the imposed condition to eliminate interfering signals generated in near-field locations. As the interference suppression only occurs at the position of near-field interference, a possible blind zone for far-field detection in conventional methods is not created. The NFFF beamformer is applicable for coherent signals and the scenario with multi interferences. For stationary situation where interferers locations are fixed, the NFFF beamformer computations do not require time updates with associated computational load. Furthermore the proposed method can be extended to several new situations such as acoustic monitoring performed from a stationary platform subjected to water currents, waves, winds and other variables, all of them generating nearby interferences and also to different array configurations including 2D and 3D arrays.     

以宏基因组技术探讨渤海秋冬季节病毒多样性

为研究渤海海域病毒群落总体状况,本文以渤海的B47站表层海水为代表,对两个时间点(2010年9月,2011年12月)的病毒宏基因组做出全面分析。采集海水样品后过滤并经过切相流系统浓缩,提取DNA测序,再进行生物信息学分析,包括病毒组的种群分类、功能基因分析、系统进化分析等。分析结果指明,这两个时间点的病毒群落均由双链DNA病毒(97.75%,scaffold百分比)占据优势地位,其中尤以有尾噬菌体(80.86%,scaffold百分比)居多。按宿主区分,最大的一类病毒为聚球藻噬藻体(10.29%,scaffold百分比)。渤海病毒群落最为丰富的功能基因为复制、结合和修复基因,且冬季(33.77%,ORF百分比)大于秋季(16.39%,ORF百分比)。结果表明:在渤海病毒组中,(1)存在理论上只出现在亚热带海域的原绿球藻噬藻体;(2)物种组成、物种多样性、功能基因比例呈现季节变化;(3)相比远洋病毒组有一定独特性;(4)可能存在未知的T4-like病毒分支。     

Submerged vegetation complexity modifies benthic infauna communities: the hidden role of the belowground system

Marine plants provide a variety of functions with high economic and ecological values in ecosystems. The above‐ (AG) and below‐ground (BG) systems increase the structural complexity of plants, which also enhance faunal abundance and diversity. The ecological role of the AG compartment in structuring inter‐tidal macrobenthic communities has been widely studied; however, this is not the case for the BG compartment. This study addressed the effects of variation in vegetation complexity (in both AG and BG systems) on associated macrobenthic infauna with respect to abundance, species richness, composition, weight and body type. To achieve this aim, a field experiment using artificial vegetation mimics was carried out using replicated treatments with different AG‐BG complexity ratios. We found a significant increase in the density and the number of taxa of macrobenthic infaunal species in plots with vegetation mimics compared with unvegetated areas, regardless of either AG or BG complexity. This effect was found even when AG parts were not present (i.e. when only BG parts were used). Furthermore, a positive relationship between structural complexity and diversity was recorded. Variation in one or both plant compartments was strongly related to diversity changes in the associated macrobenthic infauna. In conclusion, our experimental set‐up provides the first evidence that the BG compartment is at least as important as the AG compartment in controlling diversity in inter‐tidal vegetated areas because it was able to strongly affect community structure even when the AG system was totally absent.     

预处理方法对海洋微藻Isochrysis zhanjiangenisis脂肪酸组成及稳定碳同位素的影响

This study aims to quantify the effects of different pretreatment methods on the stable carbon isotope values of fatty acids in marine microalgae(Isochrysis zhanjiangenisis).To identify the effects of sample preparation on theδ~(13)C value and the fatty acid composition,we examined eight types of pretreatment methods including:(a) drying the sample followed by direct methyl esterification using HCl-CH_3OH;(b) drying the sample followed by direct methyl esterification using H_2SO_4-CH_3OH;(c) drying the sample by ultrasonic extraction and methylesterification using HCl-CH_3OH;(d) drying the sample by ultrasonic extraction and methyl-esterification using H_2SO_4-CH_3OH;(e) fresh sample followed by direct methyl-esterification using HCl-CH_3OH;(f) fresh sample followed by direct methyl-esterification using H_2SO_4-CH_3OH;(g) fresh sample with ultrasonic extraction followed by methyl-esterification using HCl-CH_3OH,and(h) fresh sample with ultrasonic extraction followed by methylesterification using H_2SO_4-CH_3 OH.The results show that the δ~(13)C values from Groups a–e,g and h fluctuated within 0.3‰,and the δ~(13)C values of Group f were approximately 0.7‰ lower than the other seven groups.Therefore,the different sample pretreatment methods used towards the extraction of fatty acids from marine microalgae may result in different results regarding the stable carbon isotope ratios,and if necessary a correction should be applied.     

风浪和海洋飞沫对海表面拖曳系数和风廓线的影响

基于埃克曼理论,本文将波致应力和飞沫应力引入到海-气边界层的界面应力中,来研究海表面风浪和海洋飞沫对海-气边界层动量交换的影响,并得到修改后的埃克曼模型的理论解。波致应力是由风浪谱和波增长函数估计,并得到在中低风速下,波致应力、飞沫应力与湍流应力相比,对海表面拖曳系数和风廓线的影响非常小。当风速高于25米/秒时,海洋飞沫通过飞沫应力对海-气界面应力的作用远高于波致应力,以至于波致应力可以忽略。海表面拖曳系数在高风速下,随着风速的增大而减小。通过采用风浪谱的不同波龄,得到海洋飞沫的产生会导致海-气边界层风速的增加。最后,理论解与现场的观察数据进行了对比。对比后的数据表明,在中高风速下,飞沫对海-气边界层的影响远大于表面风浪。     

扩散型海洋倾倒区容量估算方法初探——以嵊泗上川山疏浚物海洋倾倒区为例

以嵊泗上川山疏浚物海洋倾倒区为例,探讨扩散型海洋倾倒区容量.通过生态敏感目标水质保护要求来确定倾倒活动最大倾倒强度;以最大倾倒强度及倾倒区本身和周边海域港航功能正常发挥为前提,确定水深可淤积厚度,以此为基础,分析系列倾倒量下的区域淤积影响程度,最终确定可接受情况下的倾倒量,从而估算倾倒容量.倾倒活动产生的悬浮物影响对生态敏感目标影响分析,倾倒活动瞬时最大可接受倾倒强度至少为36 000 m3(基于实际工况);在7个月倾倒期内,设定1 500×104、4 000×104、6 000×104m3系列倾倒量,分析倾倒区本身及周边水深敏感目标的淤积程度,最终确定在1 500×104m3倾倒量下,淤积程度可接受,初步估算该倾倒区的容量为1 500×104~2 000×104m3.