Variation in microbial biomass and community structure in sediments of peter the great bay (sea of japan/east sea), as estimated from fatty acid biomarkers

Variation in the microbial biomass and community structure found in sediment of heavily polluted bays and the adjacent unpolluted areas were examined using phospholipid fatty acid analysis. Total microbial biomass and microbial community structure were responding to environmental determinants, sediment grain size, depth of sediment, and pollution due to petroleum hydrocarbons. The marker fatty acids of microeukaryotes and prokaryotes - aerobic, anaerobic, and sulfate-reducing bacteria -were detected in sediments of the areas studied. Analysis of the fatty acid profiles revealed wide variations in the community structure in sediments, depending on the extent of pollution, sediment depth, and sediment grain size. The abundance of specific bacterial fatty acids points to the dominance of prokaryotic organisms, whose composition differed among the stations. Fatty acid distributions in sediments suggest the high contribution of aerobic bacteria. Sediments of polluted sites were significantly enriched with anaerobic bacteria in comparison with clean areas. The contribution of this bacterial group increased with the depth of sediments. Anaerobic bacteria were predominantly present in muddy sediments, as evidenced from the fatty acid profiles. Relatively high concentrations of marker fatty acids of sulfate-reducing bacteria were associated with organic pollution in this site. Specific fatty acids of microeukaryotes were more abundant in surface sediments than in deeper sediment layers. Among the microeukaryotes, diatoms were an important component. Significant amounts of bacterial biomass, the predominance of bacterial biomarker fatty acids with abundance of anaerobic and sulfate-reducing bacteria are indicative of a prokaryotic consortium responsive to organic pollution.     

南极中山站大气气溶胶的化学组成及其来源的判别

从1998年3月7日至1999年11月23日历时21个月，在南极中山站连续采集89个海洋气溶胶样品，本文提供全部样品的13种化学元素Cu、Pb、Zn、Cd、Fe、Al、Mn、Cr、V、K、Na、Ca、Mg含量的实测值．研究表明中山站气溶胶化学成份的含量具有季节性变化的特征．通过相关分析、因子分析、富集因子等方法判别不同时间段中山站气溶胶化学成份的来源。     

厦门东海域定置网渔获鱼类种类组成及其季节变化

2003年7月～2004年6月对厦门东部海域的定置网渔获鱼类进行采样分析．结果表明厦门东海域至少有鱼类242种，隶属于19个目81科145属，主要是沿岸近底层和底栖的小型鱼类．以鲈形目种类最多，共计127种，占总数的52．5％、经济种类有116种．主要优势种有：青鳞小沙丁鱼、孔鳞小沙丁鱼、斑鲦、马拉邦虫鳗、裸鳍虫鳗、中华须鳗、前鳞鲻、眶棘双边鱼、多鳞鳝、皮氏叫姑鱼、短棘银鲈、列牙垒幸4、黄斑蓝子鱼、褐蓝子鱼、锯塘鳢、锻虎鱼类、褐菖鲇、绿鳍鱼、鳄鲡、卵鳎、中华单角鲍等．渔获种类在秋季及冬初最多，夏季较多，而冬末和春初最少．渔获种类的季节变化比较明显，与海洋表层水温变化有关，种类的月更替率比较高．     

烟台市污水处理深海排放工程设计方案研究与选择

沿海城市污水处理深海排放工程在我国起步较晚，烟台市污水处理深海排放工程我国目前规模最大的同类工程，且已进入施工阶段，本文详细阐述了该工程及水工设计方案，在我国城市污水２深海排放工程领域作了积极有益的探索。     

浙、闽海域仙菜科[CERAMIACEAE(RHODOPHYTA)]海藻研究Ⅰ

根据1957～1987年期间采自浙江和福建沿岸的仙菜科海藻标本，初步鉴定有7属14种。分两次报道，首先报道其中的5屑7种：复羽多孢藻Pleonosporiumvenustissimum、日本对丝藻Antithamnionnipponicum和卡洛绢丝藻Callithamnioncallophyllidicola为我国首次较全面描述和绘图记录；赫勒对丝藻Antithamnionhubbsii为浙、闽海域首次报道；而如下三个种纵胞藻Centrocerasclavulatum、日本凋毛藻Griffithsiajaponica和对公藻Antithammioncruciatum在浙、闽海区早有报道。     

Seasonal Changes in Nitrogen, Free Amino Acids, and C/N Ratio in Mediterranean Seagrasses

Abstract. Seasonal changes in nitrogen, free amino acids, and carbon were investigated in the three Mediterranean seagrasses Posidonia oceanica, Cymodocea nodosa , and Zostera noltii. Leaves, rhizomes, roots, as well as dead plant material were analysed separately. Highest N-concentrations were obtained in the winter months, regardless of species or plant part. In contrast to the other two species, the N-content in Posidonia was higher in the rhizomes than in the leaves.
In the investigated species, marked differences in the free amino acid (FAA) composition were detected between species: Proline, lacking in Posidonia , was the main component in Cymodocea. In Posidonia , FAA decreased from 320umol g^-1 (dry wt) in leaf sheaths to 1.5 umol g^-1 (dry wt) in the leaf tips. The function of proline as a possible storage and/or stress metabolite is discussed.
High C/N values in dead P. oceanica and C. nodosa rhizomes as well as in P. oceanica wrack seem to be related to considerable resistance to decomposition. Low C/N ratios did not increase in detached C. nodosa and Z. noltii leaves, which began to decompose shortly after detachment from the plants.     

Seasonal Variation in the Biomass of Marine Macrophyta from Greek Coasts

Abstract. The marine plant communities of the littoral zone in different biotopes of the Greek coasts were investigated in 1980-81. Seasonal distribution and variation of marine plant biomass were assessed. The communities of Cystoseira crinita and C. compressa were outstanding with maximum biomass during the summer months. Corallina officinalis and Pterocladia capillacea + Viva rigida communities predominated with maximum biomass in autumn and exhibited a decrease in winter, except in stressed biotopes. The species diversity and productivity of seaweeds along the Greek coasts are interpreted in relation to a number of environmental parameters.     

The Effect of Internal Fluid on the Response of Vortex-Induced Vibration of Marine Risers

Based on Iwan‘s wake oseillator model developed with the classical van der Pol equalion, the differential equation for the response of the vortex-induced vihration (VIV) of the riser considering the effect of the internal flowing fluid and the external marine environmental condition is derived. The effect of the intermal flowing fluid on the response of VIV of the riser is studied by means of the Finite Element Method. The results show that the effect of the internal fluid velocity on the VIV of the riser is strong when the natural frequency of the riser is close to the vortex shedding frequency. In addition, the increase of the top tension can decrease the sensitivity of the riser to the internal fluid velocity.     

Application of Atomic Force Microscopy to Observation of Marine Bacteria

The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as a new tool to study the fine structures of marine bacteria. This revised version was published online in July 2006 with corrections to the Cover Date.     

Probing molecular-level transformations of dissolved organic matter: insights on photochemical degradation and protozoan modification of DOM from electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

Molecular-level characterization of natural organic matter (NOM) has been elusive due to the inherent complexity of natural organic mixtures and to the fact that individual components are often polar and macromolecular. Electrospray ionization (ESI) is a “soft” ionization technique that ionizes polar compounds from aqueous solution prior to injection into a mass spectrometer. The highest resolution and mass accuracy of compounds within NOM have been achieved when ESI is combined with an ultrahigh-resolution mass spectrometer such as the Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS). With this technique, individual molecules within a variety of natural organic mixtures can be detected and their elemental composition can be determined. At low mass-to-charge (m/z) ratio, the resolution is high enough to assign exact molecular formulas allowing specific components of these mixtures to be identified. In addition to molecular identification, we can now use ESI FT-ICR MS to examine molecular-level changes in different organic mixtures as a function of relevant geo-processes, such as microbial alterations and photochemistry. Here we present the results from the application of ESI FT-ICR MS to two geochemical questions: (1) the effect of photoirradiation on the molecular composition of fulvic acids and (2) the role of protozoan grazers in the modification of DOM in aquatic systems.