一种共生藻PCR模板DNA的快速制备法

采用Chelex-100树脂法制备了26个物种(分别属于腔肠动物门、海绵动物门和软体动物门)的共生藻以及一株纯培养共生藻的DNA样品。通过PCR扩增叶绿体23S rRNA基因片段获得了26个有效序列,测序比对结果显示获得的序列与扩增目的基因相符。该方法不仅样品消耗量小、操作时间短、设备需求低廉,而且可以避免使用有害化学试剂。这是一种高效、环保的DNA提取方法,可为共生藻分子研究提供技术参考。     

南海北部冷泉区深水珊瑚的发现及其特征

冷水珊瑚也称为深水珊瑚, 在生物多样性、生态资源和科研价值等方面具有重要意义。文章对采自南海北部冷泉区的冷水珊瑚骨骼碎屑进行测定, 鉴定出冷水珊瑚2个种(Crispatotrochus sp.1和Crispatotrochus sp.2), 以及4个属[Balanophyllia (Balanophyllia)、Balanophyllia (Eupsammia)、Lochmaeotrochus和Enallopsammia]。测定的冷水珊瑚的δ¹³C为-7.36‰~-1.15‰, δ¹⁸O为-1.38‰~3.67‰, 与全球冷水珊瑚碳氧同位素组成相似, 但明显不同于南海暖水珊瑚、冷泉碳酸盐岩及低温热液成因碳酸盐岩的碳氧同位素组成。     

Dark respiration in the light and in darkness of three marine macroalgal species grown under ambient and elevated CO2 concentrations

Dark respiration (non-photorespiratory mitochondrial respiration),which occurs both in the light and in darkness,is vital for growth and survival of algae and plays a critical role in modulating the carbon balance of them.In the present study,we have investigated dark respiration in the light (R L) and in darkness (R D) in three marine macroalgal species,Hizikia fusiformis (phaeophyta),Gracilaria lemaneiformis (Rhodophyta) and Ulva lactuca (Chlorophyta),cultured at 20℃ using aeration with two CO 2 conditions:current ambient (CO 2 concentration about 380 μl/L) and elevated CO 2 (approximately 720 μl/L) air.R L was estimated by using the Kok method,whereas R D was determined as the rate of O 2 influx at zero light.The results showed that both R L and R D were unchanged for the elevated CO 2-grown algae relative to ambient CO 2 concentration for all the algal species tested.However,R L was significantly lower than R D across all the algal species and growth CO 2 treatments,demonstrating that daytime respiration was partly depressed by the light.The percentage of inhibition of respiration by light was similar between ambient and elevated CO 2 grown algae.The ratio of respiration to photosynthesis,which tended to decrease when estimated using R L instead of R D,was not altered for the elevated relative to ambient CO 2 concentration.The results suggest that R L,rather than R D,is a more accurate estimate of nonphotorespiratory carbon loss in marine macroalgae during the daytime.It would not be anticipated that elevated atmospheric CO 2 would exert a substantial influence on respiratory flux either in the light or in darkness in these particular marine macroalgal species.     

中国海相碳酸盐岩层系油气地质特点、战略选区思考及需要解决的主要地质问题

中国海相碳酸盐岩层系油气资源量大,但探明程度低,是油气资源战略接替的重要领域。中国海相碳酸盐岩层系具有形成时代老、改造强烈、沉积中心与生烃中心不吻合、储层具有多样性且非均质性强、多烃源层、多期生烃、多期成藏、多期调整与改造等显著特点,与陆相碎屑岩层系有明显差别,又有别于国外海相碳酸盐岩层系。在对不同地区海相碳酸盐岩层系油气地质条件进行分析解剖的基础上,分别对四川盆地、中下扬子及滇黔桂地区、塔里木盆地、鄂尔多斯盆地、渤海湾盆地和青藏地区的海相碳酸盐岩层系油气勘探战略选区急需解决的地质问题进行了讨论。     

“暖池”表层对大气局地强迫的响应特征

利用湍流动能垂直混合模式和ＴＯＧＡ－ＣＯＡＲＥ加强观测期的观测资料,对“暖池”上混合层的垂直混合过程进行数值试验和数值模拟,分析表层温度、盐度的变化特征。结果表明：ＴＫＥ模式可以较好地模拟混层,尤其是表层温度、盐度对大气局地强迫的响应;太阳辐射是热源,感热、潜热通量等会造成“暖池”上混合层的温度降低,“暖池”对大气释放热量;降水有利于“淡水盖”形成和维持,从则使层结稳定,ＳＳＴ升高。但在气温低于海     

高分辨率遥感技术在厦门海湾生态环境调查中的应用

对于局部区域研究，高分辨率卫星遥感及其影像的智能化处理技术是获取信息的新手段．本文以IKONOS遥感影像在厦门海湾的应用为例，探讨高分辨率遥感卫星影像应用于海湾生态环境调查与分析的应用技术．通过分析高分辨率影像地物特征，提出高分辨率遥感影像的重要处理技术：采用不同缩放尺度进行分类和利用空间特征及纹理结构进行专题信息提取。     

Impacts of systematic precipitation bias on simulations of water and energy balances in Northwest America

At high latitudes and in mountainous areas, evaluation and validation of water and energy flux simu-lations are greatly affected by systematic precipitation errors. These errors mainly come from topographic effects and undercatch of precipitation gauges. In this study, the Land Dynamics （LAD） land surface model is used to investigate impacts of systematic precipitation bias from topography and wind-blowing on water and energy flux simulation in Northwest America. The results show that topographic and wind adjustment reduced bias of streamflow simulations when compared with observed streamflow at 14 basins. These systematic biases resulted in a -50%-100% bias for runoff simulations, a -20%-20% bias for evapotranspiration, and a -40%-40% bias for sensible heat flux, subject to different locations and adjustments, when compared with the control run. Uncertain gauge adjustment leads to a 25% uncertainty for precipitation, a 20% 100% uncertainty for runoff simulation, a less-than-10% uncertainty for evapotranspiration, and a less-than-20% uncertainty for sensible heat flux.     

甲壳动物造血机制研究进展

血细胞是甲壳动物的免疫细胞,可通过吞噬、包囊、结节、黑化,以及分泌免疫活性分子等方式杀死和清除入侵的病原体。活跃的造血机能对更新和补充损耗的循环血细胞、维持一个稳定有效的免疫系统是至关重要的。相对脊椎动物而言,人们对包括甲壳动物在内的无脊椎动物的造血机制了解比较有限。本文总结了近年来在甲壳动物造血机制方面的研究进展,主要介绍了血细胞的类型和功能、造血组织的结构和细胞组成、血细胞的分化途径、造血的调控机制,以及相关的细胞模型和体外实验技术等。并在此基础上分析了已有研究存在的缺陷,提出了需要进一步探究的问题。     

夏季长江冲淡水转向机制分析

利用 1 95 9,1 975 - 1 982年及 1 998年夏季各月黄、东海盐度分布和相应风场资料 ,分析讨论了长江冲淡水夏季的扩展路径。指出 :除海底坡降外 ,由 S向的苏北沿岸流和 N,NE向的浙东沿岸流及台湾暖流构成的“力偶”,是使冲淡水向左扭转的重要外力之一。用近岸均质模型和远岸双层模型的涡度方程定性讨论了冲淡水的转向机制     

洪泽湖风生流数值模拟

湖流是湖泊中污染物质扩散，悬浮物沉降及输移等的主要水动力学要素，造成湖水运动的成因机制很复杂，其中风生流是重要形式，本文通过构造一个二维闭边界数值模拟，对不同风情下的洪泽湖湖流流态进行模拟研究，探讨其入流较小，沿岩各闸关闭的枯季情况下的风生流特征。