Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China （LCDV-cn） genome

An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene. Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309)     

岩石变形破坏的熵突变过程与破坏判据

在岩石的变形破坏过程中,当进入不稳定的破裂发展阶段之后,系统不断调整结构抵抗外力的扰动,裂纹向局域集中的有序方向发展,应变能不断耗散,并以应变能的耗散为媒介,使系统与外扰动所追加的负熵流产生能量流通,也使系统熵值和系统的维数逐次降低,这一阶段岩石系统远离平衡态,岩石的破坏是系统熵的突变过程。基于这一认识,并在验证应变能分布与结构块度分布模式的一致性的基础上,推导出包含了结构因素和能量分布的熵表达式;对熵表达式进行平衡分析获得局部突变的分岔集,得到了岩石局部破坏的熵折迭突变破坏准则;同时,探讨了熵表达式所表征的结构有序度的尖点突变性,解出岩石系统的分岔集,这个分岔集就是岩石系统熵突变的整体破坏准则。     

Finite element analysis of the effect of corrosion on the behavior of reinforced earth walls

A simple model for the corrosion‐induced loss of stiffness and strength of the steel strips of earth‐reinforced walls was introduced in a finite element simulation of the long‐term behavior of the wall, in which the backfill‐strips interactions are taken into account by means of a generalized homogenization procedure (called a multiphase model). The results show an initial phase of slow displacements induced by the loss of stiffness, followed after a few decades by a steep acceleration of the displacements, leading to wall failure. The influences of the parameter controlling corrosion, the backfill cohesion and the heterogeneity of the corrosion process are discussed. Results are used to discuss a strategy for reinforced earth wall surveillance. Copyright © 2011 John Wiley & Sons, Ltd.     

Structure of the Potential Vorticity of an Explosive Cyclone over the Eastern Asian Region in Late November 2013

An explosive extratropical cyclone(EC)over the Eastern Asian region that caused two shipwrecks is analyzed using ERA-Interim reanalysis data from the European Center for Medium-Range Weather Forecasts.Analyses of the evolution of the EC reveal that the positive potential vorticity(PV)at the upper-tropospheric level displays a hook-shaped structure during the mature period of the cyclone.The PV distribution forms a vertically coherent PV structure called a PV tower.The vertical distribution of the PV can induce and strengthen cyclonic circulation from the lower-to upper-levels of troposphere,which is an important deepening mechanism of explosive cyclone.The PV tower occurs approximately ten hours prior to the development of surface occlusion in the cyclone.The evolution of surface fronts closely follows the development of the horizontal upper-tropospheric PV.This tandem development is largely attributed to the ability of the positive upper-tropospheric PV and the PV tower to induce cyclonic circulation simultaneously.The kinematic wrap-up process of cyclonic circulation also accelerates the formation of warm occlusion.A conceptual model of the distributions of positive PV and potential temperature combining the perspectives of dynamic tropopause folding,PV tower,and atmospheric stability,including westward tilting and baroclinicity,is proposed.This model can illustrate the explosive deepening mechanism of ECs.The regions of convective instability and rainfall determined by this model are consistent with those identified from the actual observation.     

鄂尔多斯盆地南部延长组富铀凝灰岩地球化学特征及形成机制

本文系统地研究了鄂尔多斯盆地南部延长组凝灰岩元素和同位素地球化学特征。结果表明：凝灰岩主量元素具有高K、高Al、Si中等至高、低Na的特征; REE总体特征为轻稀土富集、重稀土亏损,∑REE在4857～40212 μg/g 之间变化,Eu呈现出负异常,在034～082内变化,δCe范围在080～208之间; 微量元素中U含量在312～144 μg/g 之间,Ba、U、Th、Hf和Ce呈明显正异常,Nb、P、Ti和Rb呈现负异常。详细的分析表明：U的富集和一些微量元素如Ta、Dy、Lu等相关,Th与∑REE存在明显的正相关性,这些变化可能和盆地中铀矿的富集有关; 凝灰岩的硫同位素总体呈现深源特征。根据以上的研究对凝灰岩的形成原因及形成环境进行了探讨。鄂尔多斯盆地南部延长组凝灰岩来源于秦岭造山带火山作用,其中的铀元素富集是由于砂岩型铀矿中铀向凝灰岩迁移而被还原所致。本研究对盆地砂岩型铀矿的铀运移和富集机理认识具有一定指导意义。     

Prediction of railway ground vibrations: Accuracy of a coupled lumped mass model for representing the track/soil interaction

Recent advances in railway-induced ground vibrations showed that the track/soil interaction plays an important role in the low frequency range. This paper contributes to the numerical analysis of train/track/foundation dynamics by presenting the accuracy of a coupled lumped mass (CLM) model devoted to the railway foundations and to the track/soil coupling. Following a summary of the background and the advantages of the CLM model, the coupling strategy is quantified through two application cases. Firstly, the dynamic track deflection is calculated for different railway lines considering various degrees of complexities of foundations. Then, the foundation responses are compared depending on whether detailed coupling is introduced or not. The benefit of the proposed model is emphasized by presenting free-field ground vibration responses generated by a tram and a high-speed train, obtained by a revisited two-step prediction model developed by the authors.     

苏浙皖边界地区苏皖运动的有关问题

苏皖运动的存在于否,对研究苏浙皖边界地区的地质发展史和正确认识长兴阶地层有重要意义。笔者论证了长兴组的厚度变化、界线粘土层的性质与成因、底砾岩的确认和地层接触关系、古生物群的突变等问题,认为:1)区内苏皖运动是存在的;2)砺山-江阴海堤不可否定;将安徽广德牛头山经江苏宜兴至江阴条带内的龙潭组上部地层作为长兴组的相变产物是不妥的。     

The Reference Manure Concept for Transformation Tests of Veterinary Medicines and Biocides in Liquid Manure

Veterinary medicines and biocides are frequently applied in animal houses of livestock husbandry. Due to these application patterns, they reach liquid manure. So far these substances are not transformed during manure storage, they enter soil by the application of manure as organic fertilizer. Therefore, biodegradability of veterinary medicines and biocides in manure is in the focus of regulatory procedures of environmental risk assessment. Since the representative and reproducible sampling of manures from high volume tanks is considered impossible due to high matrix variabilities, the reference manure concept was developed to utilize liquid bovine and pig manures for reproducible laboratory testing. Accordingly, excrement samples of cattle and pigs individually kept at experimental and conventional animal houses were taken. Tap water was added to matrix characterized excrements in order to prepare bovine and pig reference manures of 10 and 5% dry substance contents, respectively. Subsequently, the long‐term transformation of selected ¹⁴C‐labeled test substances was investigated under strictly anaerobic conditions. The application of different batches of bovine or pig reference manures indicated that extrapolation of transformation rates within the same animal species was possible. However, results cannot be transferred from bovine to pig manure because of substance specific interactions with the different manure matrices. The dependency of metabolic dynamics on different dry substance contents (2.5, 5, 10%) was additionally investigated in both manure matrices. These tests clearly showed that the dry substance content of the reference manure is one of the most relevant factors affecting the transformation of veterinary medicines and biocides. In contrast to highly variable tank manures, the reference manure concept allows for the exact adjustment of this parameter to guarantee reproducible laboratory testing.     

基于离散元方法的落石碰撞破碎研究

落石是山区公路边坡常见的地质灾害。落石撞击破碎是落石灾害中常见的现象,落石破碎致使运动轨迹改变往往会增大落石的危害性,但其通常未被考虑到落石的防护设计中。因此,落石破碎机理的研究对落石防护措施设计有重要的指导意义。本文运用离散元方法（离散元开源软件Esys-Particle）模拟落石铅直撞击地面的过程。在模型中,落石由大量的颗粒组成,并且相邻的颗粒由可断裂的黏结材料黏接;地面由一层固定的颗粒组成,从而模拟地面的摩擦、弹性变形性质。模型模拟出落石反弹、破碎和粉碎等过程,分析黏结材料的杨氏模量、颗粒间凝聚力和内摩擦角对落石撞击地面过程的影响,得出落石撞击过程中破坏率和动能的变化过程。研究发现：当杨氏模量和凝聚力的比值较小时,落石将会发生反弹;当杨氏模量和凝聚力的比值增大到一定程度时,落石将会破碎成不同大小的块体;当杨氏模量和凝聚力的比值超过一定限度时,落石将会破碎成非常细小的块体;内摩擦角的变化对撞击过程影响非常小。