Inhibition of Fertilization and Larval Metamorphosis of the Coral Acropora millepora (Ehrenberg, 1834) by Petroleum Products

Accidental oil spills from ships or rigs and inputs of effluent such as production formation water (PFW) are key perceived threats to tropical biota from industry activities. Scleractinian corals are an important functional component of tropical reefs and the abundance, diversity and resilience of coral communities can be used as an indicator of ecosystem health. In this paper, we report the effects of petroleum products, including water accommodated fractions (WAF) of crude oil, PFW and dispersant (Corexit 9527), on fertilization and larval metamorphosis of the widespread scleractinian coral, Acropora millepora (Ehrenberg, 1834) in laboratory-based assays. At 20% v/v PFW fertilization was inhibited by 25%. This concentration was equivalent 0.0721 mg l⁻¹ total hydrocarbon (THC). In contrast, larval metamorphosis was more sensitive to this effluent, with 98% metamorphosis inhibited at the same concentration. Crude oil WAF did not inhibit fertilization of gametes until dispersant was introduced. Dispersed oil was slightly more toxic to fertilization than dispersant alone, suggesting toxicity to that event may be additive. The minimum concentration of dispersed oil which inhibited fertilization was 0.0325 mg l⁻¹ THC. Larval metamorphosis was more sensitive than fertilization to crude oil. Although crude oil and dispersant inhibited larval metamorphosis individually, this toxicity was magnified when larvae were exposed to combinations of both. Crude oil inhibited metamorphosis at 0.0824 mg l⁻¹ THC and at 0.0325 mg l⁻¹ THC when dispersed in 10% v/v (dispersant/oil). Management of petroleum-related risks to spawning corals should consider not only the occurrence of the annual coral spawning event, but also the subsequent 1–3-week period during which most larval metamorphosis and recruitment occur.     

中国对虾(Penaeus chinensis)幼体血液循环的活体观察

溞对中国对虾早期幼体的循环系统及血液流向进行了活体观察 ,描述了中国对虾早期幼体发育阶段中幼体循环系统血管的走向与分布、血液的流向及循环路径等 ,比较各期幼体循环系统的形态 ,并探讨对虾早期幼体循环系统的发育过程。     

珠江口浮游幼虫的生态研究

根据2002—2003年珠江口12个航次的调查资料,分析探讨了不同调查时间和区域浮游幼虫的组成类型、丰度变化和环境因子的影响。以表层盐度(S)变化将调查海域划分成三个区域:I区(S<25)、II区(2530)。12个航次的调查结果显示:调查海域共出现浮游幼虫12个类型,隶属5个动物门,II区和III区的类型数一般高于I区,软体动物门和棘皮动物门的幼虫只出现在II区和III区。其中2002年7月(夏季)、2003年1月(冬季)和4月(春季)的3个大航次结果表明珠江口浮游幼虫的平均丰度为61ind/m3,三个区域的平均丰度比较为II区(124ind/m3)>I区(33ind/m3)>III区(27ind/m3),春夏季的丰度高于冬季。另外在I区进行了9个小航次调查的研究表明:I区浮游幼虫平均丰度较高,月份之间的丰度差别明显,不同站位之间也有差别。温度和食料是影响珠江口浮游幼虫丰度变化的主要因素。     

Copepods in Hypersaline Waters Worldwide:Diversity,Environmental,Social,and Economic Roles

正1 Introduction Copepods are a group of small crustaceans,which play an important ecological role in aquatic ecosystems.They occupy different ecological niches in aquatic food webs,contributes to biogenic element cycles,and transfer of organic matter from primary producers to higher secondary consumers.Copepods also have the potential to     

栉江珧工厂化育苗技术研究

通过亲贝强化营养和升温促熟培育,提早栉江珧人工育苗时间;合理控制幼虫密度,前期4~5个/mL,后期2~3个/mL,严把饵料质量关,投喂新鲜无污染的饵料,科学换水与充气,及时分级筛选等系列技术措施,克服栉江珧育苗中易发生幼虫粘连的技术难题,使育苗生产顺利进行;开展了多种附着基投放的采苗方法试验,选出了铺砂浮动网箱采苗和网袋装网片附着基和细砂吊在池中采苗2种比较理想的采苗方法,提高稚贝的附着变态率,其附着变态率达30%以上,促进稚贝的生长和成活,在1 000 m3水体中育出2 mm以上的稚贝1.096亿粒。     

珠江干流长洲水利枢纽蓄水后珠江鳡鱼(Elopichthys bambusa)早期资源现状

长洲水利枢纽是珠江干流最下游一级的梯级水坝,其正常蓄水后必然改变下游的水文环境特征,也必将对下游的鳡鱼产卵场功能造成一定的影响.为了解长洲水利枢纽蓄水后珠江鱤鱼(Elopichthys bambusa)的资源现状,2008 2013年在珠江肇庆江段设立固定采样点,通过定量弶网对鳡鱼早期补充群体进行调查.结果显示:珠江鳡鱼苗主要出现在4月中旬至8月上旬,多年平均持续时间为97±21 d,盛期在5 7月,鳡鱼苗占鱼苗早期补充群体总量的0.46%±0.21%.鳡鱼苗的出现具有明显的季节变化规律,每年伴随洪峰有3~5次苗汛出现,苗汛次数与洪峰次数之间存在显著相关关系,但苗汛峰值大小与径流量之间不存在显著相关关系.鳡鱼苗多年平均资源量为16.89×108±9.16×108尾,繁殖群体总量约为52.8 t.研究表明:长洲水利枢纽蓄水后鳡鱼早期资源量呈下降趋势,但珠江禁渔缓解了长洲水利枢纽建设对珠江鳡鱼资源的影响.基于以上结果,为长洲水利枢纽蓄水后珠江鳡鱼的保护提出了建议.     

Acute toxicities of copper,cadmium and Cu: Cd mixture to larvae of the shrimpPenaeus Penicillatus

This study showed lethal concentrations (LC) of copper forPeneaus penicillatus at various stages of its life cycle were 1000 μg/L for nauplii, 1000 μg/L for Zoea I, 2000 vg/L for Zoea II, 2500 μg/L for Zoea III, 3000 μg/L for Mysis I, II and III and that for almost 100% mortality for postlarvae was 3000 μg/L. For cadmium LC were 100 μg/L for nauplii, 500 μg/L for Zoea I, 1000 μg/L for Zoea II, 2000 μg/L for Zoea III, 2500 μg/L for Mysis I and 3500 μg/L for Mysis II, III and postlarvae. For mixture of both metals, LC were 400 μg/L for nauplii, 1000 μg/L for Zoea I, 2000 μg/L for Zoea II and 3000 μg/L for Mysis I, II, III and post larvae.     

Effects of environmental oxygen deficiency on embryos and larvae of bay scallop,Argopecten irradians irradians

The experimental results showed that: 1) The embryonic development of bay scallop is inhibited at a dissolved oxygen range of 1.38–3.64×10⁻³ at 23°C, and completely blocked below the lower limit. 2) The tolerance of larvae to anoxia increased with larval sizes and was related to their oxygen debt. 3) The scallop larvae exhibited specific behavioral responses to oxygen deficiency, which finally led to velum disintegration and larval death. The possible relationship between environmental oxygen deficiency and the disease of disintegration of the larval velum is also discussed. In this study, considerable oxygen debt was found in bay scallop larvae, which was greater in small animals. Based on the works of previous authors, a new concept is proposed for the estimation of oxygen debt, namely, the compensatory rate of oxygen debt (CROD). This can be used in intra—or interspecific comparison of oxygen debt. The results can be helpful in the management of water quality and for the prevention of larval diseases encountered in scallop culture. Contribution No. 2167 from the Institute of Oceanology, Chinese Academy of Sciences.     

ACUTE TOXICITIES OF COPPER, CADMIUM AND Cu: Cd MIXTURE TO LARVAE OF THE SHRIMP PENAEUS PENICILLATUS

This study showed Iethal concentrations (LC) of copper for Peneaus penicillatus at various stages ofits Iife cycle were 1000μg/L for nauplii, 1000μg/L for Zoea I, 2000μg/L for Zoea Il, 2500μg/Lfor Zoea III, 3000μg/L for Mysis I, II and III and that for almost 100% mortality for postlarvae was3000μg/L. For cadmium LC were 100μg/L for nauplii, 500μg/L for Zoea l, 1000μg/L forZoea II, 2000μg/L for Zoea III, 2500μg/L for Mysis I and 3500μg/L for Mysis II, III andpostlarvae. For tmixture of both metals, LC were 400μg/L for nauplii, 1000 μg/L for nauplii, 1000μg/L for Zoea I,2000μg/L for Zoea II and 3000μg/L for Mysis I, II, III and post larvae.     

几种抗菌药物对马氏珠母贝壳顶幼虫的毒性试验

几种抗菌药物对壳顶幼虫的毒性试验结果表明：（１）以９６ｈ平均日生长率作为药物对幼虫毒性的观察指标，求出日生长率仅为对照组一半时的药物浓度（９６ＴＬＭ）及安全浓度（ＳＤ）如下：氯霉素６９×１０－６、６．９×１０－６；呋喃西林７２×１０－６、７．２×１０－６；百炎净７７×１０－６、７．７×１０－６；红霉素９７×１０－６、９．７×１０－６；硫酸链霉素４８×１０－６、４．８×１０－６；青霉素钠大于１００×１０－６、１０×１０－６。（２）１３×１０－６的呋喃西林、１３×１０－６的红霉素、１３×１０－６的青霉素钠对幼虫生长均有促进作用。（３）各试验组随药物浓度递增，幼虫活动逐渐被抑制；摄食率及眼点率逐渐下降。高浓度红霉素对摄食的抑制作用最强。