Non-specific immune response of bullfrog <Emphasis Type="Italic">Rana catesbeiana</Emphasis> to intraperitoneal injection of bacterium <Emphasis Type="Italic">Aeromonas hydrophila</Emphasis>

Non-specific immune response of bullfrog Rana catesbeiana to pathogenic Aeromonas hydrophila was studied to 60 individuals in two groups. Each bullfrog in bacterium-injected group was injected intraperitoneally （i.p.） with 0.2 ml bacterial suspension at a density of 5.2 × 10^6 CFU/ml, while each one in control group injected i.p. with 0.2 ml sterile saline solution （0.85%, w/v）. Three bullfrogs in both groups were sampled at 0, 1, 3, 7, 11, 15 and 20 days post-injection （dpi） for the evaluation of non-specific immune parameters. It was observed that intraperitoneal injection of A. hydrophila significantly increased the number of leucocytes and that of NBT-positive cells in peripheral blood. Significant increases in serum bactericidal activity and serum acid phosphatase activity were also observed in the bacterium-injected frogs when compared with those in the control group. However, a significant reduction was detected in vitro in phagocytosis activity of peripheral blood phagocytes. No significant difference in changes in the number of peripheral erythrocytes, serum superoxide dismutase （SOD） activity, and lysozyme activity was detected between the two groups. It is suggested that bullfrogs may produce a series of non-specific immune reactions in response to the A. hydrophila infection.     

地质样品中微量金银的在线分离富集方法

提出用ＶＳ－Ⅱ型强碱性阴离子交换纤维定量富集、硫脲解脱，流动注射在线分离富集—火焰原子吸收光谱法测定地质样品中痕量金银的分析方法。该方法检出限低，金为１．４μｇ／ｍＬ，银为０．３５μｇ／ｍＬ，相对标准偏差均小于２％，采样频率为１２０次／ｈ，用于地质样品中痕量金银的直接测定，结果令人满意。     

水泥浆护壁堵漏若干问题的探讨

水泥浆护壁堵漏是目前钻探施工中广泛应用的技术措施之一。在水泥浆护壁堵漏施工中,因各种原因致堵漏失败,根据水泥浆护壁堵漏失败的原因,从灌注工艺方面探讨提高水泥浆护壁堵漏的效果和预防孔内事故的发生应特别注意的若干问题。     

Magmatic Sulfide Ni-Cu Deposits in China

Deep-seated magmatic liquation-injection deposits form a major type of magmatic sulfide deposit in China. The reserves of nickel and copper in this type of deposit may attain several hundred thousand tons (e.g.Hongqi 7 and Karatunggu) to nearly ten million tons (e.g.Jinchuan). Those deposits can be classified as large or superlarge deposits. The ore grade is relatively high, commonly with w(Ni)＞1 %.The mineralized intrusions are small in size, generally only 0.0n km2 to 0.n km2, with the largest one not exceeding a few km2. Before intruding, the primary magmas have undergone liquation and partial crystallization at depth; as a result, the magmas have partitioned into barren magma, ore-bearing magma, ore-rich magma and ore magma, which then ascended and injected into the present locations once or multiple times, to form ore deposits. The above-mentioned mineralizing process is known as deep-seated magmatic liquation-injection mineralization. The volume of the barren magma is generally much larger than those of the ore-bearing magma, ore-rich magma and ore magma. In the ascending process, most of the barren magma intruded into different locations or outpoured onto the ground surface, forming intrusions or lava flows. The rest barren magma, ore-bearing magma, ore-rich magmaand ore magma may either multiple times inject into the same place in which rocks and ores are formed or separately inject into different spaces to form rocks and ores. Such deep-seated magmatic liquation-injection deposits have a much smaller volume, greater ore potential and higher ore grade than those of in-situ magmatic liquation deposits. Consequently, this mineralizing process leads to the formation of large deposits in small intrusions.     

Mathematical and numerical filtration–advection–dispersion model of miscible grout propagation in saturated porous media

The development of a predictive model of behaviour of porous media during injection of miscible grout, taking into account convection, dilution and filtration of grout solution with interstitial water, as well as consolidation aspects, is presented. Model assumptions are reviewed and discussed first. During the establishment of the model, we insist on surface terms and their physical relevance in expressing adsorption effects. Constitutive laws such as Fick's law for diffusive mass transport, hydrodynamic dispersion tensor dealing with miscibility, are modified by taking into account filtration effects. A new surface term appears in mass balance equations as a consequence of filtration. According to the filtration laws used, an initial filtration rate is estimated on the basis of a one‐dimensional experimental campaign. The field equations are discretized by using Galerkin finite element and θ‐scheme standard method. For transport equation, Streamline Upwind Petrov Galerkin method is employed to prevent numerical oscillations. Lastly, confrontation of numerical results with laboratory experiments constitutes a first step to validate the model on a realistic basis. Copyright © 2001 John Wiley & Sons, Ltd.     

Injection and arrest of dykes: implications for volcanic hazards

Dykes are the principal channels through which magma reaches the surface in volcanic eruptions. For this reason dykes observed in the field are commonly assumed to be feeders to lava flows. The actual proportion of dykes reaching the surface is, however, poorly known. In order to develop models for the purpose of estimating volcanic hazard, this proportion must be known. This follows because such models should not only consider the probability of dykes being injected from magma chambers during periods of unrest in the associated volcanoes, but also the probability of the injected dykes being arrested. This paper presents field data on several thousand dykes from Iceland and Tenerife (Canary Islands) indicating that many, and probably most, dykes become arrested at various crustal levels and never reach the surface to feed eruptions. Using the results of analytical and numerical models, it is shown that, for common loading conditions, the stress field in the vicinity of a magma chamber may favour the injection and propagation of dykes while the stress field at a certain distance from the chamber favours dyke arrest. This means that many dykes that are injected from the chamber propagate only for a very limited distance from the chamber to the point where they become arrested. The implication is that during periods of unrest in volcanoes, the probability of volcanic eruption is only a small fraction of the probability of dyke injection from the source magma chamber.     

钻采天然气水合物的初步设想

天然气水合物在一定温度压力条件下能以固体形式存在,但由于其特殊的热物理力学性质,难以像开采其它固体矿产资源的＂矿山＂型式来开发。在现有技术条件下,钻探技术是解决开采天然气水合物的技术、经济可行和避免可能由开采带来的环境问题的关键。鉴于通过钻探手段将天然气水合物在地下转化为可控的资源而后开采的现实,提出了采用化学浆液注入和降压相结合的开采模式。     

福州地震台TJ-2型体积式应变仪安装及注水试验

在安装福州地震台TJ-2型体积式应变仪过程中,发现存在钻孔选点与观测室分开考虑等问题,提出一些建议。井孔注水试验结果揭示,该井孔探头顶部可能存在一个与探头近距离的含水层干扰。初步对气压、水位、降雨影响进行分析。     

钻孔扩底灌注桩施工新工艺

本文介绍钻孔扩底灌注桩的施工新工艺，多应式扩底钻头特点，成桩技术标准，适用范围及经济效益分析。     

准噶尔盆地流体输导格架及其对油气成藏与分布的控制

准噶尔盆地是一个大型叠合盆地, 不同构造单元具有不同的演化历史、流体动力学环境、流体输导格架和油气充注历史.盆地西北缘处于正常压力环境, 发育自源岩至圈闭的断裂-不整合面贯通型流体输导格架, 主要油气聚集期为三叠纪-侏罗纪.由于高效流体输导网络的发育, 西北缘油气聚集期与主力源岩生排烃期一致, 是该盆地油气最为富集的区域.盆地中部断裂密度低, 深、浅部断层被三叠系白碱滩组区域封闭层分隔, 在超压发育前和超压积蓄期为双断分隔型流体输导格架, 超压的发育导致地层发生水力破裂和封闭性断层的开启, 从而形成断裂-水力破裂连通型流体输导格架, 构成流体和二叠系源岩生成油气的穿层运移通道.由于地层水力破裂及其控制的断裂-水力破裂连通型流体输导格架的形成晚于主力源岩的主生油期, 盆地中部油气的主要聚集期晚于主力源岩的主生油期, 且原油的成熟度较高.研究证明, 输导格架控制区域性流体动力学环境、油气优势运移通道、油气的充注层位和充注历史.