氨氮胁迫对刺参几种免疫酶活性的影响

以养殖刺参(Apostichopus japonicus)为研究对象,对不同浓度的氨氮处理及病菌感染条件下刺参体腔液免疫酶的变化进行了测定.结果表明:随着氨氮处理强度的增加,刺参体腔液中超氧化物歧化酶(SOD)、碱性磷酸酶(ALP)、谷胱甘肽过氧化物酶(GPx)及溶菌酶(LSZ)活性呈现先升高后降低的趋势,而同时感染病菌的情况下,较低质量浓度的氨氮胁迫可使SOD、GPx、ALP及LSZ活性上升,但在较高浓度氨氮处理时,酶活性的诱导则受到抑制.说明适宜浓度的氨氮处理可增强刺参的免疫力,从而减轻病菌感染对刺参造成的免疫功能损伤和提高刺参抗病力.刺参在感染病原菌假交替单胞菌(Pseudoalte-romonas sp)的条件下,3 mg/L、4 mg/L和6 mg/L浓度的氨氮处理第6天,刺参的累积发病死亡率分别为44.4％、55.6％和72.2％,高于对照组,表明较高浓度的氨氮胁迫能够显著降低刺参的免疫力,增加对病原菌的易感性.因此,在刺参养殖过程中,氨氮浓度的调控具有重要的意义.     

利用ITS 基因研究徐闻滨珊瑚的系统发生关系

分别研究了徐闻3种滨珊瑚的ITS1和ITS2基因的碱基组成和G+C含量,并和已上传至Genbank上的其他9种滨珊瑚的ITS序列进行比较,研究了徐闻3种滨珊瑚的系统发生关系.序列分析结果显示,3种滨珊瑚的ITS1的长度为205 bp～209 bp,G+C含量为37.6％～45.5％,ITS2的长度为192 bp～226 bp,G+C含量为45.1％～50.7％,利用MEGA4.1软件计算滨珊瑚属基于ITS1和ITS2基因的平均遗传距离分别为0.097和0.200.基于ITS1和ITS2的NJ系统进化树都显示出,灰黑滨珊瑚位于进化树的基部,是原始的类群,普格滨珊瑚是进化类群,澄黄滨珊瑚是过渡类群.     

壳寡糖对大菱鲆生长和免疫功能的影响

以基础饲料中添加500mg/kg壳寡糖(Chitosan oligosaccharide,COS)喂养初始体质量为(12.2±0.01)g的大菱鲆(Scophthalmus maximus L.)8周,研究COS对大菱鲆生长、血清免疫相关酶活性和抵抗迟缓爱德华氏菌感染能力的影响。结果表明:饲料中添加壳寡糖后,大菱鲆成活率得到提高,特定生长率和增重率分别提高了15.79%和25.26%。相比对照组,饲料中添加壳寡糖饲养的大菱鲆血液中性粒细胞的吞噬指数提高了4.51%,酸性磷酸酶活性提高了32.89%,同时,大菱鲆抵抗迟缓爱德华氏菌感染的免疫保护力显著增强(P<0.05)。研究表明,在饲料中添加壳寡糖能够提高大菱鲆的生长、非特异性免疫功能和免疫保护力。     

核安全相关边坡与一般边坡挡土墙抗震稳定性验算对比分析

在挡土墙稳定性计算基本原理基础上, 引入《核电厂抗震设计规范》(GB 50267-97) 关于地震系数的规定, 重新建立了安全相关边坡挡土墙土压力和地震角计算公式, 并应用到实际工程中。对比分析显示, 挡土墙按核安全边坡和一般边坡进行抗震验算时, 地震系数、地震角的取值相差较大; 由于按核安全边坡计算时地震力远大于一般边坡, 因此稳定系数远小于一般边坡。      

有限灌溉对荒漠绿洲春玉米产量及产量性状的影响

对干旱环境下临泽荒漠绿洲春玉米进行了有限灌溉试验研究,旨在探究有限灌溉对绿洲玉米农田生产力的影响。结果表明,在所有处理中,全生育期灌水最少的处理MI1玉米籽粒产量比灌水量略高于它的处理MI2和灌水量较高的处理MI4增产25.7%和38.8%,但MI1与其他处理及对照间差异不显著。MI1处理全生育期灌水量最少,其产量不但未显著下降,反而高于灌水最多的对照CK。不同灌溉处理及对照间玉米穗粗、秃顶长、穗行数均不存在显著差异,但个别处理及对照间其他产量性状却存在差异,其中对照CK、处理MI5、MI1的穗长分别比处理MI4增加15.3%、13.6%和12.4%,对照CK和处理MI1、MI5的行粒数分别比MI4处理增加17.3%、13.9%和12.2%,对照CK的穗粒数分别比处理MI4、MI2和MI3增加19.3%、19.1%和14.5%,处理MI1的千粒重分别比MI4、MI5、CK增加26.9%、14.0%和14.0%。     

Consensus maps of cloned plant cuticle genes

Plant cuticle,which covers the plant surface,consists of waxes and cutins,and is associated with plant drought,cold,and salt resistance.Hitherto,at least 47 genes participating in the formation of plant cuticle have been cloned from Arabidopsis thaliana,Oryza sativa,Zea mays,Ricinus communis,Brassica napus,and Medicago truncatula;and about 85% of them encode proteins sharing above 50% identities with their rice homologous sequences.These cloned cuticle genes were mapped in silico on different chromosomes of rice and Arabidopsis,respectively.The mapping results revealed that plant cuticle genes were not evenly distributed in both genomes.About 40% of the mapped cuticle genes were located on chromosome 1 in Arabidopsis,while 20% of the mapped cuticle genes were located on chromosome 2 but none on chromosome 12 in rice.Some cloned plant cuticle genes have several rice homologous sequences,which might be produced by chromosomal segment duplication.The consensus map of cloned plant cuticle genes will provide important clues for the selection of candidate genes in a positional cloning of an unknown cuticle gene in plants.     

病态加权总体最小二乘靶向病灶的正则化方法

病态EIV模型的病灶源于设计矩阵的部分数据列之间存在复共线性关系。针对病灶特点制定正则化策略,在克服病态性的同时尽量减小正则化过程所引起的副作用,提出靶向病灶的正则化方法。通过数值试验,与总体最小二乘方法、病态总体正则化方法等进行比较,结果表明靶向病灶的正则化方法最优。     

Potential use of dissolved cyanobacterial DNA for monitoring toxic Microcystis cyanobacteria in filtered water

Toxic and non-toxic Microcystis sp. are morphologically indistinguishable cyanobacteria that are increasingly posing health problems in fresh water systems by producing odours and/or toxins. Toxic Microcystis sp. produces toxicologically stable water soluble toxic compounds called microcystins (MCs) that have been associated with cases of aquatic life and wildlife poisoning and kills including some cases of human illnesses/deaths around the world. Thus, the need for rapid detection of toxic Microcystis sp. in surface water is imperatively a necessity for early mitigation purposes. Genomic DNA from potentially toxic Microcystis sp. comprises of ten microcystin synthetase (mcy) genes of which six major ones are directly involved in MCs biosynthesis. In Polymerase Chain Reaction (PCR) methodsmcy genes can be amplified from intracellular/extracellular genomic DNA using PCR primers. However, little is known about the limitations of sourcing genomic DNA templates from extracellular DNA dissolved in water. In this work, filtered water (0.45 μM) from a Microcystis infested Dam (South Africa) was re-filtered on 0.22 μM syringe filters followed by genomic DNA isolation and purification from micro-filtrates (9 mL). Six major mcy genes (mcyABCDEG) from the isolated DNA were amplified using newly designed as well as existing primers identified from literature. PCR products were separated by gel electrophoresis and visualized after staining with ethidium bromide. The limitation of using dissolved DNA for amplification of mcy genes was qualitatively studied by establishing the relationship between input DNA concentrations (10.0–0.001 ng/μL) and the formation of respective PCR products. The amplification of mcyA gene using new primers with as little as 0.001 ng/μL of DNA was possible. Other mcy gene sensitivities reached 0.1 ng/μL DNA dilution limits. These results demonstrated that with appropriately optimized PCR conditions the method can provide accurate cost-effective tools for rapid detection of toxic Microcystis sp. in water giving early information for water quality monitoring against MC producing cyanobacteria.     

A fast clonal selection algorithm for feature selection in hyperspectral imagery

Clonal selection feature selection algorithm (CSFS) based on clonal selection algorithm (CSA), a new computational intelligence approach, has been proposed to perform the task of dimensionality reduction in high-dimensional images, and has better performance than traditional feature selection algorithms with more computational costs. In this paper, a fast clonal selection feature selection algorithm (FCSFS) for hyperspectral imagery is proposed to improve the convergence rate by using Cauchy mutation instead of non-uniform mutation as the primary immune operator. Two experiments are performed to evaluate the performance of the proposed algorithm in comparison with CSFS using hyperspectral remote sensing imagery acquired by the pushbroom hyperspectral imager (PHI) and the airborne visible/infrared imaging spectrometer (AVIRIS), respectively. Experimental results demonstrate that the FCSFS converges faster than CSFS, hence providing an effective new option for dimensionality reduction of hyperspectral remote sensing imagery.     

基于MapObjects的威海市经济技术开发区地籍查询系统的设计与实现

该文介绍了威海市经济技术开发区地籍查询系统的设计与开发过程。系统开发的主要目的是为威海市经济技术开发区提供简单的地籍管理与查询平台。系统集成了现代电子地图预览、简单的查询与分析以及3D数字威海预览等功能,充分利用了第二次全国土地利用调查的成图数据,实现了地理信息系统应有的效用。