Seasonal distribution of bacteria in salt marsh sediments in North Carolina

The number and size of bacteria at four depths (0–1, 5–6, 10–11 and 20–21 cm) in a North Carolina salt marsh were minotored by direct counts for 13 months. The number of bacteria reached a maximum of about 1·4 × 10¹⁰ cells cm^?3 at the sediment surface in October, corresponding to the period of Spartina alterniflora die-back. Cell numbers were lowest and most consistent throughout the year at the 20 cm depth of sediment. Cell volumes averaged 0·2 μm³ at the marsh surface and decreased with depth. Mean standing crop of bacteria to a depth of 20 cm of sediment was about 14 g bacterial carbon m^?2. In surface sediments bacteria contribute up to 15% and algae up to 10% of total living microbial biomass as estimated by adenosine triphosphate (ATP). Bacteria were the major biomass component at sediment depths of 5, 10 and 20 cm. At all depths the microbial community contributes < 4% total organic carbon and < 8% of total nitrogen.     

利用有益微生物改善养殖生态环境的研究

研究了噬菌蛭弧菌在实验室条件下对水产养殖中常见病菌的裂解能力及噬菌蛭弧菌和光合细菌对池塘生态环境的改善作用。结果表明 ,噬菌蛭菌对嗜水气单胞菌具有裂解作用 ,噬菌蛭弧菌和光合细菌结合使用对养殖环境具有明显的改善作用。 2 5d后实验组比对照组池塘中的细菌总数少 2个数量级 ,70d后实验组比对照组池塘中的细菌总数少 3个数量级 ;2 5d后对照组COD为 6 35mg L ,使用噬菌蛭弧菌浓度为 3、5、1 0mL m3 及光合细菌皆为 5mL m3 实验组的COD分别为 4 6 7、4 1 6、4 1 8mg L ;2 5d后对照组NH3 N为 0 4 9mg L ,使用噬菌蛭弧菌浓度为 3、5、1 0mL m3 及光合细菌皆为 5mL m3 实验组的NH3 N分别为 0 36、0 33、0 32mg L ,90d后对照组NH3 N为 0 4 1mg L ,使用噬菌蛭弧菌浓度为 3、5、1 0mL m3 及光合细菌皆为 5mL m3 实验组的NH3 N分别为 0 2 7、0 2 2、0 2 1mg L ;5 5d后对照组硫化物为0 0 1 1mg L ,使用噬菌蛭弧菌浓度为 3、5、1 0mL m3 及光合细菌皆为 5mL m3 实验组的硫化物分别为0 0 1 0、0 0 0 8、0 0 0 8mg L。     

大亚湾网箱养殖区异养细菌和弧菌的数量动态

研究了大亚湾网箱养殖水体中细菌的数量动态，以探讨其变化规律与鱼病的关系。结果表明：总细菌、弧菌的数量变化各异，网箱内、网箱外和对照海区细菌数量分布与变化不同。养殖区异养细菌丰值期在4-5月份，5月份最高；网箱水体弧菌的丰值期在6-11月，最高在8月；网箱外丰值期在7-9月，最高在8月，对照海区最高值在9月．其他时间变化不大。无论是异养菌还是弧菌，细菌值大小顺序为网箱内、网箱外、对照海区。弧菌数量与部分环境因子相关性较为密切，但相关程度随不同空间而不同，可能受其他环境条件影响。弧菌的高值期与弧菌病的流行期相吻合，鱼体受伤或其他原因引起细菌感染也会使水体中细菌数量升高。     

博格达南、北缘成盆过程演化及其对油气形成影响

以博格达地史演化为主线,探讨博格达构造域成盆—成烃—成藏的演化过程。研究认为,博格达构造域时空演化具有明显的分期性、分段性,其演化历程大体经历三个阶段:①泥盆纪—早二叠世西博格达为陆内裂谷阶段,东博格达经历泥盆纪—石炭纪裂谷演化阶段后于石炭纪末期裂谷回返形成褶隆;②晚二叠世—侏罗纪西博格达为裂谷后坳陷阶段,东博格达褶隆进一步隆升形成界山雏形,南缘进入弱压陷—挠曲沉降阶段;③侏罗纪末期—第四纪西博格达裂谷开始回返隆升成山,北缘形成阜康前陆,东博格达界山雏形再度隆起,南缘形成前陆。成盆—成烃—成藏演化史表明,西博格达经历裂谷后期坳陷阶段湖相烃源岩发育（P2）,后期（T-J）深埋,晚期（J末-Q）裂谷回返隆升造山致使油气破坏阶段,造山前期远源运聚成藏较为有利;东博格达南缘经历多期压陷-挠曲沉降,发育湖-沼相多套泥质、煤系烃源岩（P2-J2）互层,前侏罗纪构造格局控制上二叠统油气系统远源运聚成藏,侏罗纪末期以来造山运动所形成的构造格局控制中、下侏罗统油气系统源内（或近源）垂向运聚成藏。     

华北地区东部下古生界烃源岩分布特征及其发育模式

华北地区下古生界烃源岩研究对前第三系油气战略资源选区具有重要意义。从沉积学角度出发,通过对研究区有机碳含量、生烃潜力和氯仿沥青"A"等参数的统计分析和平面制图,划分了烃源岩类型,并进行了品质评价,分析了烃源岩的成因,总结了烃源岩发育模式。研究认为:华北东部奥陶系上、下马家沟组及峰峰组为较好的烃源岩,平均有机碳含量分别为0.183%、0.21%和0.181%;下寒武统发育优质烃源岩(TOC平均值为6.46%,最高值为11.2%),但分布范围局限。华北地区东部烃源岩按岩石类型不同可分为两套,即下寒武统泥质烃源岩,中、下奥陶统的碳酸盐岩烃源岩。烃源岩的成因取决于沉积环境,泥质烃源岩发育于陆棚浅海环境,而碳酸盐岩烃源岩则受控于云坪和局限台地环境。对烃源岩空间展布规律研究发现,合肥盆地为潜在的泥质烃源岩发育区,而东濮拗陷、临清拗陷、冀中拗陷为潜在的碳酸盐岩烃源岩发育区。     

河套盆地临河坳陷油气成藏过程解剖及勘探潜力分析

河套盆地属于我国中西部地区中小型陆相含油气盆地,近期于临河坳陷西南部吉兰泰构造带发现亿吨级大油田。工区地质条件复杂且勘探程度低,油藏动态演化过程研究有待深化。针对吉西凸起带和中央断垒带已发现油藏,应用地质、地震和分析化验等资料,在石油地质综合分析的基础上,采用“九史”分析方法对油藏进行动态解剖,取得了以下三点认识:(1)油气晚期成藏并表现出“三晚”,即烃源岩晚期生烃、断裂晚期活动及油气晚期充注;(2)第四纪走滑活动影响油气富集,负花状构造不发育且晚期强烈反转地区有利于成藏;(3)优选出西南部凸起带和中央断垒带两大油气有利勘探区带。     

Hydrocarbon Generation Characteristics of Source Rocks from Different Saline Environments in the Dongpu Depression,Bohai Bay Basin,China

The Dongpu Depression is a secondary salt-bearing tectonic unit in the Bohai Bay Basin, eastern China. The depositional environment of this depression regarding its Paleogene strata is clearly different in plane, including the saltwater environment(SE) in the north, the freshwater environment(FE) in the south and the brackish water environment(BE) in the middle. The result of oil and gas exploration in the Dongpu Depression shows that more than 90% of the proven oil reserves are distributed in the northern saltwater environment. Previous studies indicate that the organic geochemistry characteristics and the hydrocarbon generation capacity of the source rocks are very clearly diverse under different environments, which results in the significant differences in the proved reserves between the north and the south. In order to further explore the differences in the hydrocarbon generation capacity of the source rocks under distinct depositional environments and the mechanism of their occurrence, three samples from different depositional environments(W18-5 for SE, H7-18 for BE, CH9 for FE) were used for confined gold tube pyrolysis experiments. The results show that the CH4 yields of W18-5, H7-18 and CH9 increase with increasing temperature, the maximum yields being 405.62 mg/g TOC, 388.56 mg/g TOC and 367.89 mg/g TOC, respectively. The liquid hydrocarbon yields of W18-5, H7-18 and CH9 firstly increase with increasing temperature and then decrease after the critical temperatures. The maximum yields of C6-14 are 149.54 mg/g TOC, 140.18 mg/g TOC and 116.94 mg/g TOC, the maximum yields of C14+ being 852.4 mg/g TOC, 652.6 mg/g TOC and 596.41 mg/g TOC, respectively for W18-5, H7-18 and CH9. To summarize, the order of hydrocarbon potential from high to low is W18-5, H7-18 and CH9. On this basis, through analyzing the influencing factors of hydrocarbon differences, this paper reveals that the saltwater environment is characterized by 4 factors: higher salinity, halophilic algae, high paleo-productivity and a strongly reducing environment, which are beneficial to the enrichment of organic matter and lead to the formation of high levels of sapropelite and exinite. According to the variation of oil and gas components in the pyrolysis experiments, the hydrocarbon generation process is divided into three stages: kerogen cracking, oil cracking and C2-5 cracking. Combined with hydrocarbon generation characteristics and stages, the evolutionary model of hydrocarbon generation for source rocks under different environments is established.     

Distribution of Mid-deeply Buried Sand-bodies and their Hydrocarbon Significance at Basin Margins: Case Study of the Paleogene in the Eastern Liuzan Area of the Nanpu Sag,Bohai Bay Basin,China

The current research aims to unlock the temporal and spatial distribution of sand-bodies and their relationship with hydrocarbon accumulation in the mid-deep layer of basin margins, located in the eastern part of the Liuzan area of the Nanpu Sag, Bohai Bay Basin, China. Through this study, the main target interval of the area was divided into two fourth-order sequences. The lean sand mixed sedimentary belt with great importance in controlling hydrocarbon accumulation was identified through the comprehensive use of 3D seismic data, logging data, lithological data and seismic inversion data. A detailed analysis of sand-body development characteristics and their role in controlling hydrocarbon accumulation was conducted. The results reveal that the study area mainly develops two distinct fan-delta lobes from the NE-trending sediment supply direction. Due to the relatively high influence of the lacustrine transgression event, the scale of the lean sand mixed sedimentary belt within the SQ1-1 (the lacustrine transgressive systems tract) is relatively small, showing a relatively continuous distribution of sand bodies in the plane. Conversely, due to the relatively high impact of the lacustrine regression event, the scale of the lean sand mixed sedimentary belt developed within the SQ1-2 (the highstand systems tract) is relatively large, the two east sets of fan-delta sedimentary systems being clearly separated. The lean sand mixed sedimentary belt formed a good lateral occlusion belt in favor of hydrocarbon accumulation. Through composite analysis of structural interpretation results, sand-body distribution and well test data, it is evident that the lean sand mixed sedimentary belt located in the structural high is not a favorable zone for hydrocarbon accumulation. In contrast, the wing of the high part of the structure is the zone of sand-bearing bodies and is a favorable zone of hydrocarbon accumulation.     

南黄海盆地中部隆起中—古生界海相油气多期成藏——来自CSDP-2井的勘探启示

大陆架科学钻探CSDP-2井不仅完成了南黄海盆地中部隆起的首次钻探,也创造了陆架区2 843.18 m的全取心深钻记录。在CSDP-2井钻遇的三叠系青龙组（T₁q）,二叠系大隆组（P₃d）、龙潭组（P_2-3l）、孤峰组（P₂g）、栖霞组（P₁q）和志留系坟头组（S₁f）等多层段岩心中探获了多类型、多级别的油气显示,且二叠系3个油砂样品表明该区曾经历过大规模的油气运聚过程。地球化学分析、油源对比结果表明：1^#油砂和2^#油砂层段均表现出"近源成藏"的特征,其中大隆组1^#油砂中的原油与大隆组、龙潭组上段泥岩的亲缘关系明显,龙潭组2^#油砂中的原油与龙潭组下段、孤峰组和栖霞组烃源岩具有更好的亲缘关系;3^#油砂中的原油成熟度明显偏低,推测其可能来自两侧坳陷区的白垩系、古近系烃源岩;盆地模拟和含烃流体包裹体测温相互印证,明确了CSDP-2井揭露的四套烃源岩与三套储集层之间存在着"接力式"生烃和"波浪式"充注的多期成藏特征,同时得益于三套盖层的良好封盖,彰显了研究区较为广阔的海相油气勘探前景。     

塔里木盆地顺北地区奥陶系油气充注过程分析

综合油气地球化学、流体包裹体和构造演化资料,对塔里木盆地顺北地区奥陶系不同断裂带油气性质差异性、成藏期次及油气充注过程进行研究。地球化学分析表明,顺北地区不同断裂带油气物性和成熟度存在差异,由西向东从7号断裂带向1号断裂带原油密度变小,西部的7号带和5号带北段油气成熟度低,5号带南段和1号带油气成熟度高。包裹体分析结果表明,顺北地区奥陶系油气藏存在加里东晚期、海西晚期及喜马拉雅期多期油气充注过程。自西向东不同断裂带上主成藏期存在差异,西部的7号及5号断裂带油气成藏期为加里东晚期和海西晚期,东部的顺北1号断裂带多了一个晚期油气成藏即喜马拉雅期成藏。顺北地区多期油气充注成藏是导致该区油气成熟度变化的主要原因。研究区奥陶系成藏期古构造对断裂带的油气充注具有控制作用。加里东晚期,研究区走滑断裂带构造均处于充注有利区;海西晚期,顺北11号断裂带及其以西地区充注来自东部满加尔的油气概率较低。喜马拉雅期,仅顺北1号断裂带及其以东获得高熟油气充注。