海水网箱养殖鱼类越冬期的死亡原因及对策

在冬春季低水温期,海水网箱养殖的鱼类大批死亡造成了严重的经济损失。本文对死亡的原因进行了初步研究,结果表明:在一定的低水温环境下,这些鱼类虽然因冻伤而造成局部坏死,但维持生命的主要器官的机能仍处于基本正常状态;而患鱼继发感染细菌致病才是其死亡的主要原因。采取适当的越冬防寒措施可提高养殖海鱼在越冬期的成活率。     

Serum immunoglobulin of the mandarin fish, Siniperca chuatsi with development of polyclonal antibody

Serum immunoglobulin from the mandarin fish, or the so-called Chinese perch,Siniperca chuatsi (Basilewsky), was successfully purified using affinity chromatography. Heavy and light chains were detected on electrophoresis gel, with molecular weights being estimated at 72 and 29 kDa, respectively. The tetrameric IgM ofS. chuatsi was calculated to be 808 kDa. The rabbit polyclonal antisera against the purifed immunoglobulin were developed and tested by Western blot analysis. The antisera reacted strongly with the heavy chains ofS. chuatsi immunoglobulin. Humoral immune responses of the mandarin fish can then be examined using the developed polyclonal antibody. Project No. 30025035 supported by NSFC, State Key Laboratory of Freshwater Ecology and Biotechnology (2000FB02), and the Chinese Academy of Sciences (KZ9511-B1-111).     

中国干旱区恢复生态学研究进展及趋势评述

本文评述了中国干旱区恢复生态学研究的历程和特点,系统分析了其研究前沿和未来发展趋势.国家重大需求推动了干旱区恢复生态学研究,植被建设是生态恢复的主要方法和手段;植被分布的地带性规律、土壤生境的恢复、生态水文互馈机理、植物水分关系、生物土壤结皮、基于模型的预测模拟是未来研究的重点;地学与生物学的学科交叉融合为干旱区恢复生态学理论创新和生态恢复实践提供了新的思路和途径.     

Connections between hydrodynamics, benthic landscape and associated fauna in the Balearic Islands, western Mediterranean

Linking the abiotic and biotic traits of ecosystems is a critical step towards understanding their structure and functioning. Here we attempt to determine the connections between the hydrodynamics, benthic landscape and the associated fish communities on the coastal continental shelf off the Balearic Islands (western Mediterranean). Specifically we investigate the role the hydrodynamics play in shaping the benthic landscape, and whether the hydrodynamics affect the composition and structure of demersal fish communities. A realistic numerical model was used to establish the hydrodynamic characteristics of the area. The study area showed high hydrodynamic variability on a medium spatial scale (tens of km) in terms of mean water velocity (ū). Principal component analysis was used to determine the main gradients of macro-epibenthic variability. Redundancy analysis (RDA) was used to model the effect of the hydrodynamics on macro-epibenthic species. RDA was also used to model the effect of the hydrodynamics and macro-epibenthos on the abundance of the associated fish fauna, and on its biomass at a community level using biomass spectra classes. The results showed that the hydrodynamics had a significant influence on the distribution of both macro-epibenthic species and the associated fish species. The latter was also influenced by the macro-epibenthos. Fish size appeared to be a key attribute for the distribution of species across gradients of ū and macro-epibenthic change. Our findings can be applied in ecosystem-based fisheries management, as they show that it is necessary to take into account both the biotic and abiotic traits of the habitats when the habitat use and requirements of the associated species are defined.     

The impacts of fine sediment on riverine fish

Elevated fine sediment input from terrestrial and aquatic sources as a result of anthropogenic activity is widely recognized to impact negatively on aquatic ecosystems. In rivers, freshwater fish are exposed to a range of impacts resulting from fine sediment pressures. To date, research on the effects of fine sediments on fish has been concentrated within relatively few families, notably the salmonidae. This paper reviews the literature describing indirect and direct impacts of fine sediment on freshwater fish as a contribution towards enhancing the understanding of the impacts of anthropogenic activities on freshwater ecosystems. We identify the causal mechanisms that underpin the observed negative response exhibited by fish populations to enhanced fine sediment loads, and the variability across different fish species. Copyright © 2011 John Wiley & Sons, Ltd.     

Composition of fish species in the Bering and Chukchi Seas and their responses to changes in the ecological environment

Based on trawl surveys in the Bering Sea and Chukchi Sea during the 2010 Chinese National Arctic Research Expedition, fish biodiversity characteristics, such as fish composition, dominant species, biodiversity, and faunal characteristics were conducted. We also discussed the responses of fishes to the quick changes in Arctic climate. The results showed that a total of 41 species in 14 families were recorded in these waters. The dominant species were Hippoglossoides robustus, Boreogadus saida, Myoxocephalus scorpius, Lumpenus fabricii, and Artediellus scaber. There were 35 coldwater species, accounting for 85.37%, and six cold temperate species, occupying 14.63%. The habitat types of fish could be grouped as follows: 35 species of demersal fishes, five benthopelagic fishes, and one pelagic fish. The Shannon–Wiener diversity index(H)(range between 0 and 2.18, 1.21 on average) was not high, and descended from south to north. Climate change has caused some fishes to shift along their latitudinal and longitudinal distribution around the Arctic and Subarctic areas, and this could lead to the decline of Arctic fishery resources.     

2010-2011年长江河口及邻近水域春夏季仔稚鱼群落结构及其多样性的研究

根据2010—2011年春夏季对长江河口及邻近水域进行了仔稚鱼生态调查,应用单元和多元统计方法分析了春夏季长江河口及邻近水域仔稚鱼群落结构。4个航次调查共获得27个种类的仔稚鱼,隶属5目14科。优势种类主要包括鳀Engraulis japonicus、凤鲚Coilia mystus、康氏小公鱼Stolephorus commersonii、鮻Liza haematocheila、黄姑鱼Nibea albiflora、黄鳍刺虎鱼Acanthogobius flavimanus和寡鳞飘鱼Pseudolaubuca engraulis等。Shannon-Weaver指数(H′)在调查周期内的波动幅度较大,且2011年明显低于2010年。仔稚鱼群落结构和多样性指数春季年间较夏季年间差异性显著。调查区夏季群落结构年间相似性不高,春季群落结构格局年际变化则较明显。聚类分析表明,春夏季长江河口近岸水域仔稚鱼群落可划分为2个站位组,分别对应于长江河口淡水水域和长江口咸水水域。淡水水域组群落和咸水水域组群落总丰度与盐度相关性较高。表明盐度可能是造成长江河口仔稚鱼群落结构时空差异性的主要原因。     

DNA-based identification methods of prey fish from stomach contents of 12 species of eastern North Pacific groundfish

Stomach content analysis of eastern North Pacific groundfish has been conducted routinely by researchers interested in understanding trophic interactions between key predator species and their prey. Identification of prey by traditional morphological methods has limitations however, due to the loss of identifiable characters from digestion and morphological similarities between taxa. Furthermore, some forage fish (e.g., osmerids, ammodytids, and juvenile gadids), common prey of Bering Sea and Gulf of Alaska groundfish, are difficult to distinguish because of their slender or fusiform shape, disarticulating easily during digestion. DNA-based identification methods were developed to differentiate among 18 fish species, some that are found at depths greater than 200 m, from four taxonomic families: Ammodytidae and Osmeridae (forage fish), Pleuronectidae (flatfish), and Gadidae (gadid fish). Polymerase chain reaction (PCR) amplification of a 739 basepair section of mitochondrial DNA cytochrome c oxidase I and an 862 basepair section of mitochondrial DNA cytochrome b was followed by restriction digest assays and resulted in species level resolution for 16 of 18 species of interest. PCR restriction digest assays applied to fish prey from stomach contents of groundfish indicated the presence of several target species, eulachon (Thaleichthys pacificus), walleye pollock (Gadus chalcogrammus), searcher (Bathymaster signatus), rock sole (Lepidopsetta bilineata), yellowfin sole (Limanda aspera) and either Bering flounder (Hippoglossoides robustus) or flathead sole (H. elassodon). The PCR restriction digest protocols improved the identification rate of predated fish from stomach contents compared to identification by conventional taxonomic methods alone, and DNA sequence analysis further resolved identification of unknown prey fish samples.     

Oxygen isotopic distribution along the otolith growth axis by secondary ion mass spectrometry: Applications for studying ontogenetic change in the depth inhabited by deep-sea fishes

This study using tuna otoliths as working standards established a high lateral resolution and precision analysis to measure δ¹⁸O_otolith by secondary ion mass spectrometry. This analytical approach of the ion probe was applied to deep-sea fishes to reconstruct the likely depths inhabited by the fishes at different life history stages based on the measured δ¹⁸O_otolith values as a proxy of water temperature. Dramatic increases up to 5–6‰ in δ¹⁸O_otolith, representing a temperature decrease of approximately 20 °C, were detected in a blind cusk eel (Barathronus maculatus) otolith and in the otoliths of Synaphobranchus kaupii during leptocephalus metamorphosis to glass eel, inferred from the drop of otolith Sr/Ca ratios and increase of otolith growth increment width. δ¹⁸O_otolith profiles clearly divided the fish's life history into a planktonic stage in the mixed layer of the ocean and a benthic stage on the deep-sea ocean bottom. The habitat shift signal was recorded within a 150 µm width of otolith growth zone, which was too narrow to be clearly detected by mechanical drilling and conventional isotopic ratio mass spectrometry. However, variations down to −7‰ were found in δ¹⁸O_otolith profiles as the result of Cs²⁺ beam sputter in the core and larval portions of the otoliths. Carbon mapping by electron probe microanalyzer and staining by toluidine blue suggested abundant proteins existed in the areas with anomaly negative δ¹⁸O_otolith values, which cannot be interpreted as a habitat change but due to the isotopic fractionation by O emission from the proteins. These results implied that careful design and understanding of the chemical composition of the analytical areas or tracks on the heterogeneous otolith was essential for highly accurate and precise analysis.     

Diagnostic model construction and example analysis of habitat degradation in enclosed bay: I. diagnostic model construction

Presently, research is lacking regarding the diagnosis and evaluation of habitat degradation in enclosed bay systems. We established a diagnostic model for enclosed bay habitat degradation （EBHD model） using a multi-approach integrated diagnostic method in consideration of driving force-pressure- state-influence-response. The model optimizes the indicator standardization with annual average change rate of habitat degradation as the basic element, to reflect accurately the impact of the change and speed of degradation on the diagnostic results, to quantify reasonably the contribution of individual diagnostic indicator to habitat degradation, and to solve the issue regarding the influence of subjective factors on the evaluation results during indicator scoring. We then applied the EBHD model for the Sansha Bay in Fujian Province, China, evaluated comprehensively the situation of habitat degradation in the bay, and screened out the major controlling factors in the study area. Results show that the diagnostic results are consistent in overall with the real situation of the study area. Therefore, the EBHD model is advantageous in terms of objectivity and accuracy, making a breakthrough in diagnosis and evaluation for habitat degradation in enclosed bay systems.