从鳕鱼碎肉中提取水解蛋白

用蛋白酶水解法从鳕鱼加工废弃物－鳕鱼碎肉中提取水解蛋白。鳕鱼碎肉经枯草杆菌ＡＳ１．３９８中性蛋白酶２４０ＩＵ／ｍｌ，ｐＨ７．０，５０℃下水解２ｈ；再经黄曲霉ＩＦＦＩ２３４２（蛋白酶活力２０００ＩＵ／ｇ）１．５％（ｗ／ｖ），５０℃，ｐＨ６．０下水解６ｈ以脱苦味。水解液经真空浓缩后喷雾干燥得水解蛋白粉。该制品易溶于水，无苦味，有海鲜味。其氨基酸组成合理，是优良的蛋白质制品。     

山东蓬莱沿海的贝类

本文报导了蓬莱沿海的贝类１１１种,隶属４９科８６属 ,并对它们的生态类型,区和纱分布特点作了初步分析。     

多金属结核瞬时生长速率及其意义

本文用Ｘ射线荧光光谱分析，沿结核壳层的生长方向逐点扫描测定铁锰含量，计算结核壳层的瞬时生长速率，划分结核壳层的生长阶段，计算各生长阶段的生长速率，并在结构壳层中建立高分辨率的时间标尺，重建多金属结核生长历史。     

Biogeography, Taxonomical Status and Ecology of Alcyonium (Parerythropodium) coralloides (PALLAS, 1766)

Abstract. Ecology and morphology of Alcyonium (Parerythropodium) coralloides were studied in different sites of the Eastern Atlantic and the Mediterranean. The species is extremely variable in size, colony shape and colour. In the Mediterranean population, encrusting red colonies are mostly encountered, although lobate colonies also exist. White and pink colonies are considered mutants, which tolerate the stable environment of deeper habitats better than the conditions encountered in shallow biotopes. Mediterranean colonies are dioecious and reproduce sexually. In the Eastern Atlantic, lobate pink colonies are the most common form, and show little variability. These (at least partly) parthenogenic populations are considered offspring from stray larvae from more southern regions. This explains the rather patchy distribution of the Atlantic populations, as compared to the widespread distribution throughout the entire Western Basin of the Mediterranean and the Adriatic Sea. A study of the most important systematic characters of the species (growth form, sclerites and solenial network) leads to the conclusion that coralloides belongs to the genus Alcyonium. As a result, coralloides being its type-species, the genus Parerythropodium will have to be suppressed.     

Identification of SNARE proteins in fish-Tilapia Oreochromis niloticus

1 IntroductionSecretion is a basic function of all living organ-isms. In fish, like in mammals, neurotransmitters,hormones, enzymes and other macromolecules aresecreted in several neuronal, neuroendocrine and ex-ocrine organs and tissues located in brain,…     

海洋无脊椎动物消化酶的研究 Ⅰ、紫贻贝、日本蟳、滨螺消化酶的初步分析及其应用

本文用11种酶底物,测定了紫贻贝、日本蟳和滨螺消化系统的酶活性,并初步研究了这些消化酶的应用。实验表明:1.0％的紫贻贝消化酶在25℃、20分钟内可水解去除鲬鱼受精卵膜。1.0％的该消化酶加入等体0.5％的半纤维素酶和果胶酶,在30℃、60分钟内可制备出大量啤酒酵母原生质体。在同样条件下,1.5—2小时内,可去除大部分三角褐指藻的细胞壁。     

盐度日节律性连续变化对孔石莼生长和生化组成的影响

利用实验生态学的方法,探讨盐度日节律性连续变化对孔石莼(Ulva pertusa)生长和生化组成的影响,旨在查明潮间带大型海藻对盐度日节律性连续变化的适应性.实验共设5个盐度日节律变化处理(30±3,30±6,30±9,30±12和30±15)和1个恒盐(30±0)对照处理.实验结果表明孔石莼能够耐受较大范围的盐度日节律性连续变化,这种变化虽然对孔石莼的生长不利,但是仍然能够使孔石莼的相对生长率保持在12%以上;盐度变化幅度±3～±15与恒盐对照相比明显不利于孔石莼的生长,盐度变化幅度越大,孔石莼生长越缓慢.同恒盐处理相比,盐度日节律连续变化对孔石莼叶绿素含量的影响不明显,但叶绿素a和叶绿素b含量都有随着盐度变化幅度的增大而逐渐减少的趋势.盐度日节律性连续变化使孔石莼蛋白质和可溶性糖的相对含量显著增加(P＜0.05),随着盐度变化幅度的增大,蛋白质和可溶性糖增加的趋势越明显,并且在盐度日节律性连续变化下,蛋白质增加的速度比可溶性糖增加的速度要快.同时,游离脯氨酸的相对含量随着盐度变化幅度的增加而增加,但是在缓和的盐度日节律性连续变化下(30±3)孔石莼游离脯氨酸的含量却显著小于恒盐对照组(P＜0.05).盐度日节律性连续变化同恒定盐度相比,虽不利于孔石莼的生长,却促进了孔石莼生化产物的合成,是海藻干露时品质得以提高的原因之一.     

氮浓度对盐生杜氏藻和纤细角毛藻叶绿素荧光特性及生长的影响

在温度为(23±1)℃,盐度为31,光照强度为5000lx的条件下,用含有不同氮浓度(0μmol/L,55μmol/L,440μmol/L,880μmol/L,1760μmol/L,7040μmol/L)的培养基对中国海洋大学微藻种质库保存的盐生杜氏藻(Dunaliella salina)和纤细角毛藻(Chaetoceros gracilis)进行培养,研究两种微藻在一次性培养过程中,不同氮浓度对其PSⅡ最大光能转化效率(Fv/Fm)、叶绿素含量以及细胞密度的影响。单因子方差分析结果表明,氮浓度对两种微藻的光合作用及生长均有显著影响(P<0.05)。两种微藻的Fv/Fm比值、叶绿素含量以及细胞密度均随着起始氮浓度的增加而增加,在1760μM时达到最大值,其后随着起始氮浓度的增加,上述指标反而下降。多重比较结果表明,盐藻和纤细角毛藻进行光合作用和生长的最适氮浓度都为1760μmol/L。。