不同饲养方式对乌鳢(Ophiocephalus argus)肠道微生物群落结构差异及种类多样性的影响

以乌鳢(Ophiocephalus argus)为研究对象,采用不同日粮饲喂,试验W1组饲喂冰鲜鱼,试验W2组饲喂畜禽内脏。12周后,取肠道内容物,利用16S rDNA技术研究投喂不同饲粮的乌鳢肠道生物群落结构差异和微生物多样性,分别获得W1组和W2组乌鳢肠道细菌有效序列51124和39298条。结果分析表明;W1组和W2组物种注释(operational taxonomic unit,OTU)数目分别为2631和4114条,分类地位明确的细菌种类分别隶属5个属和49个属,W1组和W2组乌鳢肠道细菌种类差异很大。W1组乌鳢肠道细菌的优势类群是邻单胞菌属(Plesiomonas)、狭义梭菌属(Clostridium sensu stricto)、鲸蜡菌属(Cetobacterium);W2组乌鳢肠道细菌的优势类群是狭义梭菌属(Clostridium sensu stricto)、邻单胞菌属(Plesiomonas)、Spartobacteria、甲基副球菌属(Methyloparacoccus)、杆菌属(Alsobacter)、Saccharibacteria、分支杆菌属(Mycobacterium)。W2组乌鳢肠道细菌香农指数高于W1组乌鳢肠道细菌,表明前者肠道细菌多样性大于后者。本实验揭示了畜禽内脏饲喂乌鳢肠道细菌的群落种类多样性、OTU丰富度和物种总数均高于冰鲜鱼饲喂乌鳢;W1组肠道有益菌OTU丰富度大于W2组,且W2组还存在一些潜在致病菌。本研究为乌鳢的健康养殖和后续肠道微生物资源的挖掘提供了理论依据。     

Feeding intensity and molecular prey identification of the mantis shrimp,Oratosquilla oratoria (De Haan, 1844), in the Tianjin coastal zone of Bohai Bay

Bohai Bay, a representative semi-enclosed bay of China, has been severely impacted in recent decades by rapid human development. Although the mantis shrimp Oratosquilla oratoria plays an important role in the Bohai Bay ecosystem and fishery, its feeding ecology and the impact of habitat changes on its feeding habits are poorly known. In this study, we sought to identify its feeding strategy through the separation of stomach contents, and to describe its trophic ecology during maturation in the Tianjin coastal zone of Bohai Bay by the DNA barcoding method using a fragment (about 690 bp) of the mitochondrial cytochrome oxidase I (CO I) gene. A total of 594 specimens were collected from March to July 2018, and 347 (58.42%) stomachs were found to have food remains. More than half of the O. oratoria individuals had poor feeding activity, and the degree of feeding of females was higher than that of males, but there was no significant difference in the visual fullness index and the fullness weight index (FWI) between sexes for each month. In addition, the feeding activity of O. oratoria was consistent across the months, suggesting in artificial breeding programs it is not worried too much about the accessional diet supplied to the female bloodstocks except maintaining their normal food. A total of 167 retrieved prey items yielded 187 readable sequences; 86.63% of these sequences could be assigned to the species level. Consequently, 25 different prey taxa covering all readable sequences were identified, showing O. oratoria is a generalist predator. In terms of percent composition by frequency of occurrences (%N), crustaceans and fishes accounted for the same and highest percentage (35.83%) in the mantis shrimp diet, followed by mollusks (24.59%) and the remaining marine organisms (3.73%) including Polychaeta (2.67%), Sagittoidea (0.53%), and Chromadorea (0.53%). The result shows the benthic fish Oxudercidae (family) appears to be an especially important food supply for O. oratoria during maturation. The degree of cannibalism decreased with an increase in diet species and the frequencies of cannibalism in this study was noticeably higher than that in previous studies, suggesting that cannibalism may be a significant feeding strategy in the mantis shrimp O. oratoria in the Tianjin coastal zone of Bohai Bay, and because of the frequently cannibalistic behavior, the high stocking density of broodstocks should be avoided.     

甘肃西秦岭区域成矿模式

通过对西秦岭区域成矿特点和区域构造演化阶段的讨论,选择区内主要矿床就控矿地质因素、成矿时代、物质来源等特征进行对比,根据矿床成矿模式概括区域成矿模式。提出新元古代、晚古生代、晚中生代是本区大规模成矿期,对甘肃西秦岭地区成矿环境的分析认识进一步深化,对指导区域矿产评价和预测具有指导意义。     

极点对称模态分解下陕西气候变化特征及影响因素

全球变暖背景下,受人类活动和气候系统波动共同影响,气候要素响应具有非线性、非平稳特征,如何识别气候变化多时间尺度信息,是当前研究的热点话题.基于1970—2017年气温和降水逐日数据,辅以滑动平均、趋势分析和极点对称模态分解(ESMD)等方法,对陕西3大地理单元气候时空特征进行分析,进而探讨不同海区厄尔尼诺指数与气温、...     

雷达-雨量计联合估测区域降水量方法研究Ⅰ——模式集成法的提出

天气雷达估测区域降水量是其应用研究的重要领域,不断研究改进雷达估测区域降水量精度和稳定度是雷达气象工作的重要目标。文章回顾了集合预报理论基础及其发展概况,总结分析集合预报方法、产品表现形式等。对前期雷达估测区域降水量的8种方法,从基本原理和特点等方面进行了系统分析。对该理论在雷达估测区域降水领域的适用性进行了分析,阐述了集合预报理论在该领域应用的概念模型,并提出了两种集成估测区域降水量的新方法,为下一步的应用研究提供了理论支撑。     

天津近岸海域大型底栖动物摄食功能群特征及其与环境因子的关系

摄食功能群是指生态系统中食性相同的生物群落,研究摄食功能群有助于科学评估区域生态健康状况.基于2015年9月、2016年5月和2016年9月的天津近岸海域大型底栖动物调查数据,结合同步获取的环境因子,分析研究区大型底栖动物摄食功能群组成及其变化特征,探究大型底栖动物摄食功能群组成及变化的原因.结果表明,3个航次的大型底...     

基于水化学特征的“奥灰”地热流体水文地球化学演化机制研究——以济南东部章宁1地热井为例

以章宁1地热井的研究为例,在分析地热水水文地球化学特征基础上,对地热水的化学成分形成进行了研究,并通过与相对补给径流区的西曹范、章桃1地下水水化学特征的比较,研究其演化机理。综合分析认为地热水为沉积岩溶滤水,属于中度变质水,地下水流经火成岩侵入体时溶解了大量的Cl~-及F~-,HSiO~-_3等微量元素,次为地下水与沿文祖断裂上升的深部热液的混合,导致地热水化学类型的改变。深部热液的水化学成分与济南东地热水与深部热液的混合比例有待进一步研究。     

基于EMD方法的地心运动时间序列分析

针对时间序列混有的高频信息会影响地心运动规律分析的问题,采用网平移法对IGS提供的GNSS周解进行解算,得到2007-2017年地心运动时间序列,对其进行分解重构,剔除高频项,并利用重构时序对地心运动规律作进一步分析探讨。结果表明：本文解算的地心运动在T_x、T_y和T_z方向的精度均为毫米级。EMD方法重构的时序保留了原序列的基本信息,且抑制了高频项的影响,提高了周期贡献率,3个方向的贡献率分别提高了12.3%、16.7%及6.3%。通过分析重构后的时序发现,周年项振幅为各周期对应振幅的最大值,分别为2.32、1.89和2.07 mm;T_y和T_z方向长期变化趋势较T_x更为明显,分别为0.13和-0.27 mm/a;半年项较小,且在T_x和T_y方向上具有时变性。此外,还发现了一些其他较小的年际变化。     

Consumption of Ecosystem Services in Laos

The exponential increase of ecosystem utilization has instigated a serious conflict between ecosystem services and residents’ needs. The Belt and Road Initiative has greatly influenced Laotian production and living, and the scientific assessment of the consumption of ecosystem services in Laos is important for exploring residents’ influence on the ecosystem. Based on data for the Laotian consumption of agricultural products, fruits and livestock products during 1961-2013, normalized by either harvest index or feed conversion ratio, this study draws three main conclusions. 1) Ecosystem service consumption in Laos is centered on the consumption of farmland, forestry and grassland ecosystem services, which account for over 80%, over 10% and under 2%, showing downward, upward and constant trends, respectively. The consumption of these ecosystem services shows a trend of increasing first, then fluctuating, and finally increasing. 2) The consumption of ecosystem services in Laos was characterized by the mode of “dominance of consumption of the farmland ecosystem services” from 1961 to 2008, and the mode of “balanced development of consumption of farmland, forest and grassland ecosystem services” from 2008 to 2013, with a trend of transformation from the former into the latter. 3) The formation and change in the consumption mode of Laotian ecosystem services have been affected by both supply and trade. Laos developed agriculture mainly during the period from 1961 to 2008, forming the mode of “dominance of consumption of the farmland ecosystem services”. This development benefited from the enriched varieties of imports as well as the increased value of trade and import volume. However, the consumption of ecosystem services in Laos after 2008 changed from the mode of “dominance of consumption of the farmland ecosystem services” to one of “balanced development of consumption of farmland, forest and grassland ecosystem services”. This study provides an empirical reference for research on the consumption of ecosystem services.     

黑海参(Holothuria atra)对环境沉积物的摄食选择及消化系统功能适应性研究

黑海参(Holothuria atra)是热带珊瑚礁大型底栖动物群落中的代表种类之一,其摄食活动对珊瑚礁生境底质有机物的循环再利用具有重要作用。本文研究了三亚蜈支洲岛典型热带珊瑚礁海域野生黑海参的沉积物选择特征及其触手和消化系统结构功能的适应性。结果表明,黑海参摄取的沉积物颗粒相对较粗,其中粒径0.25mm部分占到了总重量的96.67%。触手上3—6个乳突形成乳突簇[直径(283.00±40.94)μm],适宜摄取大颗粒沉积物。黑海参胃内含物有机物含量显著高于环境沉积物,证实其对有机物含量的选择性;粒径0.25mm部分有机物含量占摄入总有机物量的75%,与环境沉积物中相同粒度的有机物总量占比接近(76%),表明黑海参选择的沉积物粒径范围有效保证了其高效摄入有机质。黑海参前肠的脂肪酶、淀粉酶、纤维素酶、胰蛋白酶活性最高,同时绝大部分的有机质(98.33%)在前肠被吸收。组织学定量分析表明,黑海参前肠黏膜层厚度最大,占总厚度的56.33%,这与其高效吸收有机质的功能相适应;后肠的肠壁总厚度和黏膜下层、肌层厚度均显著高于前肠和中肠,表明其具有相对更好的弹性和收缩能力,适合于大颗粒有机质的高效输送与排出。研究结果证实,黑海参摄食器官和消化道独特的结构与功能保证了其在珊瑚砂粗颗粒生境中高效摄取有机物。