The chemical changes of DOM from black waters to coastal marine waters by HPLC combined with ultrahigh resolution mass spectrometry

How dissolved organic matter (DOM) undergoes chemical changes during its transit from river to ocean remains a challenge due to its complex structure. In this study, DOM along a river transect from black waters to marine waters is characterized using an offline combination of reversed-phase high performance liquid chromatography (RP-HPLC) coupled to electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS), as well as tandem ESI-FTICR-MS. In addition, a water extract from degraded wood that mainly consists of lignins is used for comparison to the DOM from this transect. The HPLC chromatograms of all DOM samples and the wood extract show two major well-separated components; one is hydrophilic and the other is hydrophobic, based on their elution order from the C₁₈ column. From the FTICR-MS analysis of the HPLC fractions, the hydrophilic components mainly contain low molecular weight compounds (less than 400 Da), while the hydrophobic fractions contain the vast majority of compounds of the bulk C₁₈ extracted DOM. The wood extract and the DOM samples from the transect of black waters to coastal marine waters show strikingly similar HPLC chromatograms, and the FTICR-MS analysis further indicates that a large fraction of molecular formulas from these samples are the same, existing as lignin-like compounds. Tandem mass spectrometry experiments show that several representative molecules from the lignin-like compounds have similar functional group losses and fragmentation patterns, consistent with modified lignin structural entities in the wood extract and these DOM samples. Taken together, these data suggest that lignin-derived compounds may survive the transit from the river to the coastal ocean and can accumulate there because of their refractory nature.     

厄尔尼诺现象和浙江近海鲐鲹鱼渔获量变化关系──长江口ENSO渔场学问题之二

本文研究浙江近海1970年到1992年鲐鱼渔获量变化与Elnino现象的关系，结合捕捞强度，长江中下游汛期（4—9月）降水量距平年际变化综合分析，探讨浙江近海鲐鱼渔获量变动规律，为合理开发渔业资源提供科学依据。     

近岸Ⅱ类水体表层悬浮泥沙浓度遥感模式研究进展

因为具有明显的时间与空间分辨率优势,遥感数据成为近岸Ⅱ类水体悬浮泥沙浓度(SSC)信息提取研究的重要数据源之一.悬浮泥沙遥感信息提取的现状可归纳为:(1)建立近岸Ⅱ类水体SSC遥感模式的方法有三种类型,分别是基于地面光谱与SSC测量的反射率反演方法、基于图像信息法和基于大气辐射传输理论模型法;(2)基于地面测量的反射率反演方法属于理论与经验相结合的方法,也是目前用于SSC定量化遥感模式研究的常用方法.其数学表达形式包括线性关系式、对数关系式、负指数关系式、Gordon模式和综合模式等;(3)到目前为止已有的Ⅱ类水体SSC遥感模式适用性方面还不理想,远未达到与试验室分析相匹配的精度.文章认为:加强地面水文光谱实验研究,建立多光谱SSC定量模式,以高分辨率和高光谱遥感融合数据为基础的SSC定量遥感是今后该方向发展趋势.     

Estuarine and coastal connectivity of an estuarine-dependent fishery species,Pomadasys commersonnii (Haemulidae)

Understanding the level of connectivity between estuarine and coastal waters is essential for appropriate management of estuarine-associated taxa. Most studies have focused on the role of a single estuary, while limited research exists on the importance of multiple estuaries to individuals of estuarine-associated species. This study used acoustic telemetry to assess the usage of multiple estuaries and coastal waters by the estuarine-dependent spotted grunter Pomadasys commersonnii. Twenty-six adult fish were tagged with acoustic transmitters in the Kariega and Bushmans estuaries, South Africa, and their movements along a 300-km stretch of Indian Ocean coastline were monitored for up to 17 months. Tagged individuals spent most of their time in the estuary where they were tagged (55% and 85% for fish tagged in the two estuaries, respectively), followed by time in the sea (30% and 15%) and in other estuaries (15% and <1%). The mean durations of sea trips for fish tagged in the Kariega Estuary or Bushmans Estuary, respectively, were 25 days (range 3–55) and 12 days (range 2–22). Of the fish that went to sea, 93% from the Kariega Estuary and 60% from the Bushmans Estuary visited other estuaries. Most visits were undertaken to the Swartkops, Bushmans and Kowie estuaries, although the longest durations were spent in the Sundays Estuary. Individuals moved to estuaries up to 130 km away. The total distance travelled between estuaries by an individual during the study was ～529 km, with means of 201 and 184 km, respectively, for fish tagged in the Kariega and Bushmans estuaries. Despite covering large distances between estuaries, individuals often returned to their tagging estuary. Residency in their tagging estuary, combined with frequent visits to a neighbouring estuary, highlights the importance of estuarine habitats for this popular fish species, even after reaching maturity.     

The birth of the Paratethys during the Early Oligocene: From Tethys to an ancient Black Sea analogue?

Deeper water black shales, overlain by coccolith-bearing marlstones representing the incipient Paratethys (example: Early Oligocene; Austrian Molasse Basin), have sedimentary characteristics similar to those of the Holocene Black Sea since 7500 years bp. Framboid pyrite size, biomarker and C–N-isotope data additionally indicate that isolation of the Paratethys resulted in Black Sea-type characteristics during nannoplankton zone NP 23.In contrast to the estuarine circulation across the Bosphorus since 7500 years bp, marine conditions prevailed in the incipient Paratethys during NP 21/22. Nitrogen was fixed and low organic carbon accumulation rates prevailed. In both settings a vertical density water-column stratification was accompanied by photic zone anoxia, and by anaerobic methane oxidation in the Paratethys. In the Paratethys increased run off, starting in NP 22, led to estuarine circulation during NP 23. During this period cyclic blooms of calcareous nannoplankton resulted in high calcite accumulation rates which diluted the coeval clay sedimentation. Similar sedimentary features in the Black Sea and the Paratethys during the earliest Oligocene are result from opposite paleoceanographic developments, both leading to estuarine circulation patterns. In the Black Sea, permanent photic zone anoxic conditions were established 7500 years bp in response to the first invasion of saline Mediterranean waters into the former freshwater lake. In contrast, brackish surface water in the Paratethys resulted from nutrient-rich freshwater diluting the marine water body.     

九孔鲍养殖水体中产酶菌群的研究

为了解九孔鲍养殖环境中细菌生理菌群的生态作用,对分离自养殖水体的33株菌株的产酶能力和种类进行了研究。结果表明,具有分泌胞外蛋白酶、脂肪酶和纤维素酶能力的菌株分别为39.4%、57.6%和30.3%;在这33株菌中,产至少一种胞外酶的菌株比例高达63.6%。API鉴定结果表明,在有能力分泌胞外蛋白酶、脂肪酶和纤维素酶的菌株中,弧菌菌群所占的比例分别为46%、42%和40%,而其中河流弧菌又占各弧菌菌群比例的83.3%、62.5%和100%。充分阐明弧菌,尤其是河流弧菌为优势菌,在鲍鱼养殖微生物态中起的重要作用。     

Detecting subtle hydrochemical anomalies with multivariate statistics: an example from ‘homogeneous’ groundwaters in the Great Artesian Basin,Australia

The major ion composition of Great Artesian Basin groundwater in the lower Namoi River valley is relatively homogeneous in chemical composition. Traditional graphical techniques have been combined with multivariate statistical methods to determine whether subtle differences in the chemical composition of these waters can be delineated. Hierarchical cluster analysis and principal components analysis were successful in delineating minor variations within the groundwaters of the study area that were not visually identified in the graphical techniques applied. Hydrochemical interpretation allowed geochemical processes to be identified in each statistically defined water type and illustrated how these groundwaters differ from one another. Three main geochemical processes were identified in the groundwaters: ion exchange, precipitation, and mixing between waters from different sources. Both statistical methods delineated an anomalous sample suspected of being influenced by magmatic CO₂ input. The use of statistical methods to complement traditional graphical techniques for waters appearing homogeneous is emphasized for all investigations of this type. Copyright © 2006 John Wiley & Sons, Ltd.     

Kinetics of phosphate adsorption by iron oxyhydroxides in aqueous systems

The rate of uptake of phosphate onto synthetic Fe(III)- and Fe(II)-derived oxyhydroxides has been studied using reaction conditions similar to those encountered in natural waters. Kinetic analyses were carried out on the adsorption profiles and both first-order and second-order conditional rate constants were obtained. The temperature dependence of some of the rate constants was investigated and corresponding apparent activation energies calculated. Similar experiments and analyses were undertaken using Fe from natural sources and in general the conditional rate constants obtained in seawater were in agreement with the synthetic ones. The results of this study are of value when comparing the time scales of adsorption processes in natural waters with the time scales of mixing and advection.