大黄鱼病原副溶血弧菌拮抗菌的筛选

用点种法从大黄鱼肠道优势菌群中筛选出104株病原性副溶血弧菌的拮抗菌,进一步研究拮抗效果最好的11株拮抗菌的抗菌谱和生长曲线,其中NAC平板上筛选的4株拮抗菌的抗菌范围较广,锰营养琼脂培养基上筛选的3株拮抗菌具有生长优势。用BIOLOG板测定了抗菌谱较广、有一定生长优势3株拮抗菌(R22,Y58和J312)的碳源利用情况,结果表明,3株拮抗菌的碳源谱都比较广,都能利用糖类、有机酸和氨基酸等,而且与鱼类营养的竞争较小。16S rRNA基因同源性分析的结果显示与R22,Y58,J312同源性达99%以上的菌株分别来自弧菌属、芽孢杆菌属和假单胞菌属。进一步对R22,J312和Y58三株拮抗菌进行生化鉴定,结果显示R22为溶藻弧菌,J312为荧光假单胞菌,但Y58的种属未能得到确认。所得到的3株拮抗菌R22,Y58,J312在拮抗病原菌、抗菌谱、生长特性和营养利用等方面都初步满足益生菌的筛选条件,有待进一步检测其安全性和实用效果以便能够成为能实用的益生菌。     

Tension during parametric excitation in submerged vertical taut tethers

The construction of a suspension bridge with floating pylons or a submerged floating tunnel requires the installation of a mooring system. The option of taut vertical tethers, similar to those used in tension-leg platforms, has been suggested in preliminary designs. The environmental loading on the tether, mainly due to wind waves and swell, results in a parametrically excited system. Certain loading conditions develop instabilities that translate into large horizontal motion. However, the effects of parametric resonance on the tension values have rarely been investigated. This paper aims to clarify the relation between lateral displacement and tether tension and to quantify the extreme tension values in the event of parametric resonance. The presented analysis is based on a full numerical model of the tether that includes geometric and hydrodynamic nonlinear effects. This model is used to investigate a representative example that illustrates parametric resonance and multiple parametric studies to assess the effects of the excitation frequency, amplitude, initial pretension, tether length and inclination angle on the tether’s response. The results reported here provide the basis for a recommendation on designing a tether under parametric resonance regarding the ultimate extreme values and fatigue life.     

准南霍玛吐背斜带天然气成因类型及来源

准噶尔盆地南缘霍玛吐背斜带经历了复杂的构造变动和演化,地质条件非常复杂,对油气成藏条件特别是油气成因一直存在争议。通过对目前已发现气藏的天然气组分、碳同位素、轻烃组成等特征分析,探讨了霍玛吐背斜带天然气成因类型及来源。组分分析结果表明,霍玛吐背斜带天然气为有机成因湿气,主要为腐殖型干酪根裂解气;碳同位素分析结果表明,霍玛吐背斜带天然气主要为煤型气,既有热解气,也有裂解气;轻烃分析结果表明,霍玛吐背斜带天然气为典型煤型气。综合成因类型分析结果认为,该区天然气主要来源于侏罗系烃源岩的煤系地层,既有热解气,也有裂解气。     

西湖凹陷孔雀亭气田成藏主控因素

综合运用地质、钻井、生物标志物、碳同位素、储层流体包裹体等资料,在油气分布特征和来源分析的基础上,探讨了控制西湖凹陷孔雀亭气田油气成藏及富集的主要因素。研究结果表明,孔雀亭气田油气主要分布在始新统平湖组储层内,具有"上油下气"的纵向分布特征,以断块型凝析气藏为主,原油及天然气来源于自身和西部次洼平湖组源岩联合供烃。孔雀亭气田油气成藏及富集主要受断层封堵性、砂体厚度和储层物性及流体充注历史的联合控制,断层封堵性控制了油气藏的含油气性,砂体厚度和储层物性制约了油气层厚度和含油气饱和度,流体充注历史决定了油气藏的现今赋存相态。     

The Sediment Source and Transport Trends around the Abandoned Yellow River Delta,China

Sediment source and transport trends are influenced by various hydrodynamic factors, and thus play important roles in sedimentary evolution and coastal stability. To examine sediment transport trends around the abandoned Yellow River delta promontory and its erosion mechanism, we employ empirical orthogonal function (EOF) analysis to study sedimentary characteristics and transport trends of the abandoned Yellow River delta in northern Jiangsu Province, China. The results showed that: (1) the main sediment source in the abandoned Yellow River delta is the submarine coastal slope and both sides of the abandoned Yellow River Delta; (2) the main hydrodynamics controlling sediment transport is the current that runs along the shore, coupled with waves, especially southward currents; (3) the sediment of the study area was redistributed under hydrodynamics; coarse sediments were eroded and broadly transported to the south. Therefore, it is concluded that the sediment sources and transport have important influence on coastal evolution: the sediment source area shows mass loss of deposits and erosion; deposits in the submarine coastal slope provide the source and were continuously eroded to provide materials to other places as a sediment source.     

Simulating the influence of various nutrient sources on hypoxia off the Changjiang River Estuary

Hypoxia is increasingly reported off the Changjiang River Estuary with the confluence of multiple high volume nutrient sources. The Regional Ocean Modeling System coupled with a biological model was used to analyze the effect of different nutrient sources on the development of hypoxia off the Changjiang River Estuary. By comparing to observed data, our model suitably captured the regional dynamics of chlorophyll a, dissolved oxygen, and nutrient concentration. A series of sensitivity experiments were conducted to investigate the hypoxia response to the various nutrient sources, such as loading from the Changjiang River, Kuroshio and Taiwan Warm Current. Our model results indicated that nutrients from different sources significantly influenced the hypoxia off the Changjiang River Estuary, and it was mostly affected by nutrients sourced from the Kuroshio. The nutrients input from the Changjiang River had larger impacts on the hypoxia in the north of 30°N than that in the south of 30°N. The nutrients sourced from the Taiwan Strait had a least influence on the hypoxia off the Changjiang River Estuary.     

Facies recognition of some Tertiary coals applied to prediction of oil source rock occurrence

Coals are oil source rocks in many of the Tertiary basins of Southeast Asia. The precursors of these hydrogen rich and oxygen poor coals are coastal plain peats which have mainly developed in an everwet and tropical climate. In these environments water flow and reworking can concentrate liptinitic kerogen in preference to vitrinitic kerogen. The distribution, petrography and chemistry of the coaly Miocene source rocks present in the Kutai Basin are described. The recognition of environmental controls on the accumulation of potentially oil-prone coals and coaly shales in deltaic environments is an aid to predictive source bed recognition in petroleum exploration. Comments on the environment of deposition of coaly sediments in the basins of the Norwegian Sea are discussed with reference to their possible oil and/or gas sourcing potential. The Triassic - Jurassic coals of the Haltenbanken area may become more oil-prone towards the delta margins, and facies mapping could aid oil exploration in this area.     

陆相湖盆海侵事件对烃源岩发育影响机制

海侵是影响湖盆烃源岩发育的重要事件之一,通过调研国内外相关文献,述评了这一领域的研究现状。海侵事件广泛分布于中国的松辽盆地、渤海湾盆地和苏北盆地以及国外多个盆地,海侵可通过古遗迹标志、矿物岩石学标志以及地球化学特征等识别。其次海侵烃源岩一般具有有机质丰度较高、有机质类型较好的特征,主要归因于海侵事件明显促进烃源岩发育,海侵作用一方面可能为湖水带来了大量的营养物质,显著提高了古湖泊生产力,另外一方面,盐度可能有利于形成稳定的水体分层,在水底形成强还原环境,促进有机质保存。以西非裂谷盆地（Termit盆地）为例,总结了海侵影响湖相烃源岩发育的模式。     

台湾海峡表层沉积物中重矿物特征及其物质来源

台湾海峡重矿物平均分布0.95%,其分布由西岸向东岸递减;由北部经台湾浅滩朝粤东方向缓慢递减。碎屑矿物46种,以角闪石、绿帘石、磁铁矿、钛铁矿、褐铁矿和片状矿物为主,它们占重矿物含量的67%。据主要重矿物含量将海峡分成8个矿物组合区。碎屑矿物来自海峡两岸基岩,通过河流搬运入海,有部分片状矿物为海峡以北的物质随浙闽沿岸流进入海峡而沉积。其海绿石是台湾西岸、澎湖岛第三系或第四系含海绿石岩层剥蚀后搬运入海沉积而成的。台湾浅滩的残留沉积物为晚更新世低海面时期的河口滨海相沉积物。由于处在开放的海洋环境,至今没有接受现代沉积。残留沉积物中的磁铁矿、钛铁矿、锆石等重矿物相对富集,含量高于近源海域,有可能赋存浅海砂矿。