Application of Atomic Force Microscopy to Observation of Marine Bacteria

The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as a new tool to study the fine structures of marine bacteria. This revised version was published online in July 2006 with corrections to the Cover Date.     

南海东北部末次冰期以来的沉积环境演变

利用南海东北部上陆坡处所取的D孔柱样进行硅藻分析,结合粒度与碎屑矿物分析结果,探讨该海域末次冰期以来沉积环境演变过程.结果表明：粒度与硅藻分析结果较为吻合,共同显示D孔柱样可分为上下两层.上表层（0～2 cm）的砂质沉积层为冰后期海进过程改造的晚更新世残留沉积层,即变余沉积;下层（2～130 cm）主要是末次冰期形成的浅海沉积.整个柱样可反映出海洋同位素MIS 4期海退至MIS 3期海进与MIS 2期海退至冰后期海进的沉积变化韵律.     

不同水温对文昌鱼性腺发育的影响

研究表明，不同水温对文昌鱼性腺发育分化和成熟有十分显著的影响。１４℃对文昌鱼性腺发育没有显著的作用，２０和２５℃十分有利于性腺发育和成熟，经４０ｄ培养，出现一些雌、雄文昌鱼产卵和释精以及加速性腺发育。本文还讨论了水温促进性腺发育可能的机制。     

长江口枯季悬沙粒度与浓度之间的关系

2003和2004年枯季在长江口采集水样并作水文观测，对所获水样进行过滤和粒度分析，以计算悬沙浓度和悬沙粒度分布。结果表明，2003年11月小潮期间，悬沙中值粒径与悬沙浓度存在着显著的指数关系，在大潮期间没有显著关系；在2004年2月小湖期间，两者之间没有显著关系，但在大潮期间存在着显著的指数关系。枯季水体悬沙以粉砂组份为主，并且随着向口外的推移，细颗粒组份逐渐增加，但在拦门沙最大浑浊带附近，由于絮凝作用，沉积物粒度变幅较大，可产生粒径粗化的现象。小潮期间，砂含量较低，但与悬沙浓度之间有显著相关关系；大潮期间，悬沙粒径粗化，但砂含量与悬沙浓度之间的关系不显著。上述分布趋势与沉积物来源、当地的水动力条件和絮凝作用等因素有密切关系。