A Multi-Generation Schmakeriapoplesia Culturing System for Use in Ecotoxicological Study

Crustacean zooplankton form the keystone link between primary producers and fish stocks in marine and estuary, ecosystems. We have established a multi-generation cultivation system for zooplankton ruth which future experiments on the biological effects of pollutants in marine and estuary environments can be better performed. A population of calanoid copepod, Sehmakeria poplesia, was collected in December 2003 and maintained in a static system through all stages （eggs to adults）. The population ex- hinted an average developmental time of 13.6 d in conditions corresponding to the natural environment （water temperature 20~C salinity 15）. A series of experiments were performed to examine copepod egg production and hatching success as functions of food type and feeding concentration. Results in our study showed that Isoehrysis galbana was more favored for the reproduction of cope- pods than Phaeodactylum tricornutum, and 10xl0%ellsmL-1 was the most practical algae concentration. We have demonstrated that the Schmakeria poplesia population can be maintained in the laboratory through multiple generations. In addition, methods to control egg production through changes in food concentration have been established, making it feasible to control the start date of exposure experiments or the timing of the collection of oftspring to initiate a new generation.     

A multi-generation Schmakeria poplesia culturing system for use in ecotoxicological study

Crustacean zooplankton form the keystone link between primary producers and fish stocks in marine and estuary ecosystems. We have established a multi-generation cultivation system for zooplankton with which future experiments on the biological effects of pollutants in marine and estuary environments can be better performed. A population of calanoid copepod, Schmakeria poplesia, was collected in December 2003 and maintained in a static system through all stages (eggs to adults). The population exhibited an average developmental time of 13.6 d in conditions corresponding to the natural environment (water temperature 20°C, salinity 15). A series of experiments were performed to examine copepod egg production and hatching success as functions of food type and feeding concentration. Results in our study showed that Isochrysis galbana was more favored for the reproduction of copepods than Phaeodactylum tricornutum, and 10×10⁴cells mL⁻¹ was the most practical algae concentration. We have demonstrated that the Schmakeria poplesia population can be maintained in the laboratory through multiple generations. In addition, methods to control egg production through changes in food concentration have been established, making it feasible to control the start date of exposure experiments or the timing of the collection of offspring to initiate a new generation.     

卤虫休眠卵的若干生物学特性研究进展

结合作者的研究工作对卤虫休眠卵的耐性、形态结构、胚胎发育及细胞(核)数目、以及卤虫休眠卵的生化适应性等方面的国内外研究成果及现状进行了综述,并提出应该在现有的研究基础上对Clegg的假设、卤虫卵内hsp70的分子结构特征、卤虫卵的卵壳结构及卤虫卵亚壳层的功能等方面进行深入研究,为卤虫休眠卵的基础生物学研究积累资料.     

黑鲷（Sparus macrocephalus）成熟精,卵和精子入卵早期过程的初步观察

黑鲷精子入卵早期发育迅速。精、卵相遇的3－5sec左右,卵孔管出现一种能引诱精子入卵的物质;精卵相触约10－15sec左右,即精子进入卵孔管后,管内又涌出另一种能阻止多余精子继续入卵的物质;同时,受精锥形成。约15-54sec左右,受精膜发生;约90sec左右受精膜举起,围卵腔雏成;约180sec左右受精锥开始退缩,精子头部和颈部被俘入卵内;约300sec左右出现第二极体。文中对卵孔管出现的这两种物质进行了浅析。     

Occurrence of organochlorine pollutants in the eggs and dropping-amended soil of Antarctic large animals and its ecological significance

Persistent organochlorine pollutants (POPs) are analyzed for the dropping-amended soils from the habitats of Antarctic seabirds and seals in Fildes Peninsula and Ardley Island. The concentration ranges are 0.21 to 3.85 ng/g for polychlorinated biphenyls (ΣPCBs),0.09 to 2.01 ng/g for organochlorine pesticides (ΣDDT),and 0.06 to 0.76 ng/g for hexachlorocyclohexanes (HCHs). Among these,hepata-chlorobiphenyls,hexachlorobiphenyls,p,p′-DDE and α-HCH compounds are dominant. The concentra-tion ranges of ΣPCB,DDT and HCH in the eggs of skuas were 91.9―515.5 ng/g,56.6―304.4 ng/g and 0.5―2.0 ng/g respectively; those in the eggs of penguins were 0.4―0.9 ng/g,2.4―10.3 ng/g and 0.1― 0.4 ng/g; and those in the eggs of giant petrel were 38.1―81.7 ng/g,12.7―53.7 ng/g and 0.5―1.5 ng/g respectively. The dominant POP compounds in the eggs are PCB180,PCB153,p,p′-DDE and hexa-chlorobenzene (HCB). The present study shows that the concentration of POPs in the sea-bird-inhabited-dropping-amended soil varies with the extent of predation and nest occupancy of dif-ferent seabird populations. Statistical analysis on the POP concentrations from the different seabird eggs implies that the difference in the bio-concentration levels of the birds depends on the bio-habits of the species,such as the range of activity,distance of immigration,feeding pattern,and nest occupation. Among these,the most important factor is the location of the seabirds in the food chain and their feeding pattern. This shows that POPs accumulated in the seabirds resulted from the bio-concentration through the food chain. In addtion,210Pb dating for the dropping-amended soils (AD1-a and AD2) was performed,which provided the POP accumulation rate and the historic record for the soil profile. It indicates that POP will continuously affect the Antarctic ecosystem for a long time.     

大亚湾核电站温排水对邻近水域鱼卵、仔鱼的影响

大亚湾核电站运行以后 ,温排水主要影响核电站以东沿岸水域 ,温升 2℃的范围大约在2km以内。邻近水域的鱼卵和仔鱼数量仍然比较丰富 ,季节变化亦无大异常 ,但是在种类组成上出现了一些异常现象 :小沙丁鱼Sadinellaspp .鱼卵和仔鱼数量明显增多 ,斑Clupan odonpunctatus和鲷科 (Sparidae)鱼类的鱼卵和仔鱼数量显著减少 ,并且未见鱼Engraulisjaponicus和小公鱼Anchoviellasp .鱼卵以及科 (Engraulidae)和银汉鱼科 (Atherinidae)仔鱼的出现。鱼卵的数量分布与温排水有关 ,而仔鱼与之无关 ,鱼卵死亡率与温排水的关系亦不显著。文中还讨论了温排水的影响范围、鱼卵死亡率与温排水的关系以及温排水对成鱼的影响等问题。     

浙江白垩纪恐龙与恐龙蛋化石的时空分布

浙江省恐龙化石地质遗迹调查表明,全省有9个白垩纪陆相红层盆地产恐龙和恐龙蛋化石,至今已发现有4科5属5种恐龙骨骼和7科11属24种恐龙蛋化石。浙江省恐龙和恐龙蛋化石主要分布在天台盆地和金衢盆地的东阳、衢州、龙游、金华、浦江、兰溪一带,主要产于天台群的赖家组,衢江群的金华组、衢县组。永康、南马、新昌、嵊州、仙居、诸暨、湖山等地永康群朝川组、方岩组中也有较丰富的恐龙化石。依据恐龙和恐龙蛋化石在盆地内地层中的分布、地层的同位素年龄和相伴生的其他生物群特征等分析表明,恐龙和恐龙蛋化石的赋存地质时代以晚白垩世为主。此外,恐龙和恐龙蛋化石在浙江的广泛分布表明该地区地质历史时期气候温暖,植被茂盛,水资源丰富,是恐龙及其他生物生存繁衍的理想场所。     

揭开翼龙性别及生殖之谜

翼龙是已知最早演化为强有力飞行能力的脊椎动物。最近发现的一成年雌性翼龙与她的蛋保存在一起的化石, 提供了翼龙性别的直接证据,并加深了对这些已绝灭的飞行动物生殖生物学的理解。这一雌性达尔文翼龙的发现使我们对这一物种有了更多的了解。新的发现证实了雄性具有相对小的骨盆和大的头骨脊, 而雌性则具有大的骨盆和头骨无脊。对蛋化石的详细研究显示翼龙的生殖策略, 如多数研究者以前怀疑的那样, 与鸟类的是不一样的, 而与鳄类或者其它的爬行动物一样。与以前发现的具有胚胎的翼龙蛋的壳一样, 这一研究也证实与其母体相比, 蛋相对小而软且具有羊皮纸一样的壳。很明显, 蛋产下来后, 会从周围环境中吸收水分, 从而在这一时期增加其大小和重量。这意味着母体不必在蛋里投入过多的水分, 而这一点可能对于飞行来说是相当有利的: 在母体中小而轻利于携带。这也暗示了雌性达尔文翼龙像爬行动物那样很少关注她们的蛋, 这些蛋被埋着而从周围吸收营养。     

三角褐指藻和亚心形扁藻对太平洋纺锤水蚤繁殖与生化指标的影响

采用实验生态的方法,探讨三角褐指藻(Phaeodactylum tricornutum)、亚心形扁藻(Platymonassubcordiformis)对太平洋纺锤水蚤(Acartia pacifica)繁殖及其生化指标的影响.实验结果表明,三角褐指藻对太平洋纺锤水蚤产卵量和卵的孵化率均具有抑制作用,随着投喂时间的增加,太平洋纺锤水蚤的产卵量由15.02±3.15 ind/d下降到3.25±3.15 ind/d(p<0.05),其卵的孵化率由22.88%±2.49%下降到14.84%±1.20%(p<0.05).而投喂亚心形扁藻的太平洋纺锤水蚤其产卵量和卵的孵化率与对照组无显著差异(p>0.05).投喂三角褐指藻后,太平洋纺锤水蚤的超氧化物歧化酶(SOD)活力相对于投喂亚心形扁藻的增加了20.94±0.04μg/mg,其谷胱甘肽过氧化物酶(GPX)活力增加了15.812±1.240 U/mg.这表明相对于亚心形扁藻,三角褐指藻对太平洋纺锤水蚤的抗氧化性酶具有诱导作用.