二色桌片参酶解液对转化细胞的效应

用二色桌片参（Mensamariaintercedens）酶解液处理转化的人胚肺成纤维细胞（NLF）后，细胞生长受到显著抑制，抑制率达64．84％，光镜下细胞透光性加强，铺展较好，细胞核形更为规则，核畸形现象减少；线粒体结构趋于正常；软琼脂集落实验表明，细胞集落形成率由23ｘ10－5下降至12．5ｘ10－5，裸小鼠致瘤实验显示抑瘤率为82．39％，差异显著（p＜5％）。结果提示，二色桌片参酶解液可能逆转转化的HLF细胞的恶性表型，对其有一定的诱导分化作用。     

Nutrients and zooplankton composition and dynamics in relation to the hydrological pattern in a confined Mediterranean salt marsh (NE Iberian Peninsula)

The role of the hydrological regime in the nutrients and zooplankton composition and dynamics has been analysed in five lagoons of La Pletera salt marshes (NE Iberian Peninsula) during a complete hydrological cycle (2002–2003). Two of the lagoons have their origin in the old river mouths while the other three were recently created in the framework of a Life Restoration project. This fact has also allowed us to study the effect of the lagoon age on nutrient and zooplankton composition and dynamics. The salt marsh hydrology is determined by a prolonged period of confinement without water inputs, irregularly interrupted by sudden water inputs due to flooding events (sea storms or intense rainfalls). While the dynamics of oxidized nitrogen compounds in the lagoons depends on the water inputs variability within each hydrological cycle, the internal load of phosphorus, total nitrogen and organic matter is related more to the cumulative mechanisms during the confinement periods. Accumulation processes may be easily related to lagoon age, since old lagoons have higher content of nutrients and organic matter, suggesting that these lagoons progressively accumulate nutrients during the successive confinement events. This is the usual case for most Mediterranean salt marshes without an artificially manipulated water regime. The zooplankton community in La Pletera integrates the effects of both the hydrological regime and the lagoon age since the former determines the temporal pattern of the main zooplankton species and the latter explains differences in composition and structure between old and new lagoons.     

用含氯化镉培养液培养塔胞藻的生物学效应

在塔胞藻培养液中加入不同浓度的CdCl2,然后分析了培养在各种处理和对照组的塔胞藻后代.结果发现,随着镉离子浓度的升高,细胞的生长繁殖量和体积在前2轮(9d为一轮)培养中均明显降低,而在第3轮培养时,细胞的繁殖和大小接近常态.随后对处理后多种细胞内含物含量进行了分析,显示出:随着培养液中镉离子浓度增高,细胞内的蛋白质、叶绿素含量均明显下降,而可溶性糖、核酸的含量与对照相比变化不大.