魁蚶(Scapharca broughtonii)半乳糖凝集素(SbGal)基因cDNA的克隆及表达分析

半乳糖凝集素(Galectin)作为动物凝集素家族的一员,是一种重要的免疫因子,在脊椎动物和无脊椎动物中都参与了重要的免疫防御反应。为明确魁蚶(Scapharca broughtonii)半乳糖凝集素基因的结构特征、组织分布及其对病原菌刺激的免疫反应规律,本研究从构建的魁蚶转录组文库中筛选得到半乳糖凝集素基因部分序列,通过c DNA末端快速扩增(rapid amplication of c DNA ends,RACE)技术获得该基因c DNA全长序列(命名为Sb Gal)。序列和结构分析表明,该c DNA全长3120bp,编码554个氨基酸,是一个含有4个糖识别结构域(CRD)的且在双壳贝类中较为独特的半乳糖凝集素;预测蛋白的分子量(Mw)为63.3158 k Da,理论等电点(p I)为4.99。同源性及系统分析表明,Sb Gal基因与美洲牡蛎、合浦珠母贝、海湾扇贝galectin的同源性分别为70%、65%、46%,Sb Gal基因四个CRD彼此的相似性为36%—41%。实时荧光定量PCR(q RT-PCR)结果表明:Sb Gal m RNA在魁蚶血细胞、斧足、闭壳肌、外套膜、鳃和肝胰腺中均有表达,血细胞中的表达量最高,在肝胰腺中的表达量最低;注射鳗弧菌后,相对于对照组,Sb Gal基因在所检测的每个组织中m RNA水平上的表达量都显著上调(P0.01),而且表达量具有显著的时间依赖性和瞬时表达趋势,呈现先升高后降低的趋势。     

双壳类分子生物标志物对海水重金属的响应评述

海洋双壳类动物的组织和器官对微量重金属具有高富集能力,诱导机体内的某些分子生物标志物(molecular biomarkers,MBMs)发生变化,可用于指示海水中的重金属污染水平。近10年来,许多学者在双壳类MBMs对海水重金属的响应规律方面进行了研究,其中,金属硫蛋白(MTs)、热激蛋白70(HSP70)、抗氧化防御系统等受到了较多的关注。本文在介绍这些MBMs结构和功能的基础上,重点评述了各种MBMs对海水重金属的响应特征,并提出了影响双壳类MBMs应用于海水重金属监测的因素及进一步研究的方向。     

基于GIS的黄河上游龙羊峡库区生态环境遥感监测研究

本项研究以黄河上游生态环境严重退化的龙羊峡库区为研究区，应用遥感与GIS一体化的方法，建立了库区生态环境动态监测信息系统。在系统的支持下，利用多时相、多波段TM遥感数据，对库区近十年来生态环境变化进行监测分析，揭示了库区土地沙漠化、水土流失、土地利用／土地覆盖的现状，以及建库近十年来生态环境时空演化的过程与发展趋势，为库区生态环境治理提供科学依据。本项研究利用遥感和GIS技术，采用生态环境综合分类系统，在一次分类处理中通过多层次不同级别的组合获得了基于同一信息源的土地沙漠化、草场盖度、土地利用处地覆盖等多种环境类型信息，并利用环境信息模型对库区的土壤侵蚀进行了评价。基于GIS的分类后再处理使遥感分类精度有了一定程度的提高。研究表明，在GIS的支持下充分利用遥感信息监测与评价生态环境，是区域生态环境定量化研究的有效途径。     

高分辨率遥感技术在厦门海湾生态环境调查中的应用

对于局部区域研究，高分辨率卫星遥感及其影像的智能化处理技术是获取信息的新手段．本文以IKONOS遥感影像在厦门海湾的应用为例，探讨高分辨率遥感卫星影像应用于海湾生态环境调查与分析的应用技术．通过分析高分辨率影像地物特征，提出高分辨率遥感影像的重要处理技术：采用不同缩放尺度进行分类和利用空间特征及纹理结构进行专题信息提取。     

动荷载作用下海底粉土的孔压响应及其动强度

本文选用近海分布较广的粉土为研究对象 ,利用室内动三轴试验结果 ,找出动荷载作用下粉土的动应力应变关系 ,分析模拟波浪荷载作用下粉土中的孔压响应、临界循环次数 ,确定波浪作用下粉土的应力状态、破坏临界循环次数 ,判断不同深度处的粉土发生液化的可能性及发生液化所需要的时间 ;研究粉土在动荷载作用下的强度降低 ,为海上工程设计和施工提供科学依据。     

High‐resolution seismic monitoring of instrumented buildings using a model‐based state observer

This paper proposes a model‐based state observer to perform high‐definition response estimation in partially instrumented building structures. The proposed estimator is verified in a simulated five‐story shear‐building structure and validated using measurements from a seven‐story reinforced concrete building slice tested at the NEES‐University of California at San Diego shake table. In both cases the proposed estimator yielded satisfactory results by estimating the time history of shear forces, bending moments, displacements, and strains at various points/sections of interest. The proposed algorithm can be used in instrumented buildings for various practical applications such as post‐earthquake damage assessment, structural control, and building code calibration. Copyright © 2016 John Wiley & Sons, Ltd.     

海洋生态文明研究进展及其评价体系探究

海洋生态文明作为生态文明建设的一部分,是全面落实"五位一体"总体布局的重要着眼点。本文全面梳理比较了国内海洋生态文明建设的理论研究进展与实践探索,从概念内涵、应用领域、评价指标、示范区建设、实践经验五个方面梳理了现有研究,并采用重要性指数对现有研究中海洋生态文明评价指标的重要性程度进行排序,结合前期研究成果及当前形势,构建了海洋生态文明评价指标体系,并探讨了今后的研究方向。     

潮汐对瞬态气候响应模拟的影响研究

In this study, the impact of oceanic processes on the sensitivity of transient climate change is investigated using two sets of coupled experiments with and without tidal forcing, which are termed Exp_Tide and Exp_Control,respectively. After introducing tidal forcing, the transient climate response(TCR) decreases from 2.32 K to 1.90 K,and the surface air temperature warming at high latitudes decreases by 29%. Large ocean heat uptake efficiency and heat storage can explain the low TCR in Exp_Tide. Approximately 21% more heat is stored in the ocean in Exp_Tide(1.10×10~(24) J) than in Exp_Control(0.91×10~(24) J). Most of the large ocean warming occurs in the upper 1 000 m between 60°S and 60°N, primarily in the Atlantic and Southern Oceans. This ocean warming is closely related to the Atlantic Meridional Overturning Circulation(AMOC). The initial transport at mid-and high latitudes and the decline in the AMOC observed in Exp_Tide are both larger than those observed in Exp_Control. The spatial structures of AMOC are also different with and without tidal forcing in present experiments. The AMOC in Exp_Tide has a large northward extension. We also investigated the relationship between AMOC and TCR suggested by previous studies using the present experiments.     

细基江蓠繁枝变型对水体硝态氮变化的生理响应

通过静态模拟实验,比较研究了6个硝态氮(NO3--N)摩尔浓度水平(0,10,20,40,60,80μmol/L)下培养的细基江蓠繁枝变型(Gracilariatenuistipitatavar.liuiZhangetXia)的丙二醛(MDA)、游离脯氨酸含量以及硝酸还原酶(NR)、超氧化物酶歧化酶(SOD)、过氧化物酶(POD)活性的变化。结果表明,随着水体NO3--N摩尔浓度的逐渐增加,细基江蓠繁枝变型NR、SOD和POD的活性均呈现先增加后下降的显著变化(P<0.05),膜脂过氧化产物MDA含量随水体NO3--N摩尔浓度的变化则呈相反的趋势(P<0.05);游离脯氨酸含量则与水体NO3--N摩尔浓度呈正相关(r=0.9161)。综合分析结果认为,单独以硝态氮为氮源时,20μmol/L的供氮水平能使细基江蓠繁枝变型处于相对最佳的生理状态。     

Wave-induced porewater pressure in a seabed with inhomogeneous permeability

We have developed an analytical treatment for the wave-induced response of a seabed with inhomogeneous permeability, by employing the boundary layer approximation. By assuming an exponential function for vertically distributed permeability, the inhomogeneous consolidation equation was formulated as Bessel's differential equation. Subsequently, we proposed some representative quantities for examining the effects of inhomogeneity of permeability. The main effects of inhomogeneity are that the boundary layer becomes thinner and the phase delay in the direction of depth becomes larger as the inhomogeneity becomes significant. In addition, it was shown that the boundary layer is properly evaluated by using the averaged permeability.