Responses of phytoplankton and heterotrophic bacteria in the northwest subarctic Pacific to in situ iron fertilization as estimated by HPLC pigment analysis and flow cytometry

To verify the hypothesis that the growth of phytoplankton in the Western Subarctic Gyre (WSG), which is located in the northwest subarctic Pacific, is suppressed by low iron (Fe) availability, an in situ Fe fertilization experiment was carried out in the summer of 2001. Changes over time in the abundance and community structure of phytoplankton were examined inside and outside an Fe patch using phytoplankton pigment markers analyzed by high-performance liquid chromatography (HPLC) and flow cytometry (FCM). In addition, the abundance of heterotrophic bacteria was also investigated by FCM. The chlorophyll a concentration was initially ca. 0.9 μg l⁻¹ in the surface mixed layer where diatoms and chlorophyll b-containing green algae (prasinophytes and chlorophytes) were predominant in the chlorophyll biomass. After the iron enrichment, the chlorophyll a concentration increased up to 9.1 μg l⁻¹ in the upper 10 m inside the Fe patch on Day 13. At the same time, the concentration of fucoxanthin (a diatom marker) increased 45-fold in the Fe patch, and diatoms accounted for a maximum 69% of the chlorophyll biomass. This result was consistent with a microscopic observation showing that the diatom Chaetoceros debilis had bloomed inside the Fe patch. However, chlorophyllide a concentrations also increased in the Fe patch with time, and reached a maximum of 2.2 μg l⁻¹ at 5 m depth on Day 13, suggesting that a marked abundance of senescent algal cells existed at the end of the experiment. The concentration of peridinin (a dinoflagellate marker) also reached a maximum 24-fold, and dinoflagellates had contributed significantly (>15%) to the chlorophyll biomass inside the Fe patch by the end of the experiment. Concentrations of 19′-hexanoyloxyfucoxanthin (a prymnesiophyte marker), 19′-butanoyloxyfucoxanthin (a pelagophyte marker), and alloxanthin (a cryptophyte marker) were only incremented a few-fold increment inside the Fe patch. On the contrary, chlorophyll b concentration reduced to almost half of the initial level in the upper 10 m water column inside the Fe patch at the end of the experiment. A decrease with time in the abundance of eukaryotic ultraphytoplankton (<ca. 5 μm in size), in which chlorophyll b-containing green algae were possibly included was also observed by FCM. Overall, our results indicate that Fe supply can dramatically alter the abundance and community structure of phytoplankton in the WSG. On the other hand, cell density of heterotrophic bacteria inside the Fe patch was maximum at only ca. 1.5-fold higher than that outside the Fe patch. This indicates that heterotrophic bacteria abundance was little respondent to the Fe enrichment.     

南大洋深海嗜冷菌2-5-10-1及其低温脂肪酶的研究

从南大洋普里兹湾的水样中筛选到一株产低温脂肪酶的深海嗜冷菌2-5-10-1,对其生长及产酶情况和酶性质做了初步研究.该菌株的最适生长温度为5℃,此时分泌的胞外脂肪酶最多;添加Tween80,橄榄油可显著促进脂肪酶的产生.该脂肪酶的最适作用温度为35℃,在0～20℃均保持较高的酶活性,在0℃可保持37%的相对酶活性;酶的最适作用的pH值为7.5,在pH6～9的范围内均存在较高酶活性;对热较敏感,在60℃保温15min可丧失50%以上的酶活性.该脂肪酶的催化作用不需要金属离子的参与,Cu2+和Zn2+对酶活有着强烈的抑制作用.     

Responses of carbon dioxide in western tropical Pacific to 1991~1993 ENSO event

On the basis of the data of the partial pressure of CO₂ (P_CO2)and the concentration of the total dissolved CO₂(T_CO2) in surface water during the expeditions in Nov.-Dec. 1991, the world ocean circulation experiment (WOCE) and Oct. 1992-Mar. 1993, the tropical ocean-global atmosphere coupled ocean-atmosphere response experiment (TOGA COARE) in the western tropical Pacific and of the comparison with data from 1986 to 1990 TOGA expeditions and that from Japan Meteorological Agency, the response of CO₂ in surface water to ENSO event is proved. The CO₂ signals indicated that the air-sea system in the western and central tropical Pacific from 1991 to 1993, except for a short period in autumn of 1992, was in a strong state of ENSO.
The change of CO₂ in the floating stations near 2°S, 155°E from Nov. 1992 to Mar. 1993 reflected the change of currents, water mass and its thermal and salt content during the forming and developing of ENSO.     

Aspects of Deep Ocean Mixing

The turbulent motions responsible for ocean mixing occur on scales much smaller than those resolved in numerical simulations of oceanic flows. Great progress has been made in understanding the sources of energy for mixing, the mechanisms, and the rates. On the other hand, we still do not have adequate answers to first order questions such as the extent to which the thermohaline circulation of the ocean, and hence the earth's climate, is sensitive to the present mixing rates in the ocean interior. Internal waves, generated by either wind or flow over topography, appear to be the principle cause of mixing. Mean and eddy flows over topography generate internal lee waves, while tidal flows over topography generate internal tides. The relative importance of these different internal wave sources is unknown. There are also great uncertainties about the spatial and temporal variation of mixing. Calculations of internal tide generation are becoming increasingly robust, but we do not know enough about the subsequent behavior of internal tides and their eventual breakdown into turbulence. It does seem, however, that most internal tide energy flux is radiated away from generation sites as low modes that propagate over basin scales. The mechanisms of wave-wave interaction and topographic scattering both act to transfer wave energy from low modes to smaller dissipative scales. This revised version was published online in August 2006 with corrections to the Cover Date.     

A thermal centroid or a mass centroid?

This study reveals the physical backgrounds of the geometric centroid and the thermal centroid of the Western Pacific Warm Pool (WPWP) and points out their differences. The geometric centroid (actually a very close approximation to the mass centroid) anomaly of the surface WPWP correlates more closely with the Niño-3 region sea surface temperature anomaly (Niño-3 SSTA, an important indicator of El Niño/La Niña events) than the surface thermal centroid. Taking the WPWP depth (or heat storage) into account, the “real” mass or thermal centroid of the WPWP might correlate better with the El Niño/Southern Oscillation (ENSO) signals.     

Evaluation of the ocean governance system in Korea

Considering the small land, dense population and poor natural resources, the oceans are important to Korea, but the recognition of oceans has been weak in Korean society. So ocean governance was fragmented in both institution and policies, which resulted in serious problems in the coastal waters and oceans. However, a series of maritime accidents and failures of policies increased the recognition of the coastal waters and resources and led to the establishment of one single ocean-related governmental agency in 1996. Over the last decade, the ocean governance in Korea has been successful and strong.     

太平洋波高分布及变化规律研究

使用 Topex/ Poseidon卫星高度计 1 992年 1 0月～ 1 998年 1 2月连续 75个月 ,2 30个重复周期的有效波高资料对南北太平洋的有效波高进行了统计 ,分析了太平洋有效波高的多年平均、多年各月平均和多年各季平均的空间分布特征和时间变化规律。结果表明 ,太平洋波高分布具有明显季节变化的规律 ,与太平洋的风速分布特征具有良好的对应关系     

Spatial variability in growth and reproduction of the Pacific oyster Crassostrea gigas (Thunberg, 1793) along the west European coast

The Pacific oyster Crassostrea gigas was introduced in Europe for commercial purposes in the mid 1960s. It was initially thought that low winter temperatures would restrain this species' reproduction and settlement; however, its present distribution in areas where no introduction has taken place suggests that natural invasion and expansion has occurred. Along the European coast, wild populations of Pacific oysters are already found from northern Germany to southern Portugal. Whether C. gigas will continue to further expand through northern waters will depend on its physiological performance. In this study, the performance of wild oyster populations has been studied in terms of growth and reproduction at three stations: La Rochelle (France; 46°N), Yerseke (Oosterschelde estuary, The Netherlands, 51°N), and Texel (Wadden Sea estuary, The Netherlands, 53°N). The French population had the lowest somatic-shell mass ratio and an increase in maximum shell length, somatic and gonadal mass was observed from France to the Netherlands. In addition, mean oocyte diameter decreased significantly from south to north. The combination of increasing gonadal mass and decreasing oocyte volume suggests an increasing reproductive output in terms of egg numbers from France to The Netherlands. Differences in temperature between locations will at least be partly responsible for the observed patterns; however, other environmental factors (such as food availability, predation pressure, sediment type and/or seston concentration) cannot be excluded. Since smaller eggs (oocytes) are thought to have a longer development time, the environmental conditions along the Dutch coast may result in increased larval dispersal and possibly in further population expansion.     

The contribution of nano- and micro-planktonic assemblages in the surface layer (0-30 m) under different hydrographic conditions in the upwelling area off Concepción, central Chile

In the highly productive region off central Chile, the structure and temporal and spatial variability of planktonic assemblages, and the factors that determine changes in this structure are poorly understood. In the region, wind-driven upwelling, heating by solar radiation and freshwater inputs are highly seasonal processes, which, together with higher frequency events, can promote changes in the planktonic communities, especially in the upper layer. This study focuses on the structure of nano- through to micro-planktonic assemblages (2-200 μm) of unicellular organisms (protists) in surface waters (0-30 m) during different hydrographic conditions. Samples were taken from a fixed shelf station off Concepción (COPAS time series Station 18) on eight occasions between September 2003 and August 2004. The nano-plankton flagellate-dominated fraction was numerically important during the whole period. Maxima in flagellate abundance and biomass occurred during the upwelling period (November-April samplings) but these maxima appear to be unrelated to the degree of water column stratification. The micro-plankton diatom-dominated fraction was usually the largest component in terms of biomass during the study period and the diatoms made important numerical contributions during the upwelling period, with maxima in abundance and biomass when water column stability was lowest. The dominant genera and morphotypes in each functional group were found throughout the study period, with maxima in abundance and biomass co-occurring under similar environmental upwelling conditions. The mean macro-nutrient concentrations (nitrate and silicate) were relatively high in the top 30 m during both upwelling and non-upwelling periods, and did not explain the maxima in plankton or functional group replacements. The persistence of the dominant taxa in the planktonic assemblages suggests a high degree of flexibility, though probably not at the specific level, to withstand the highly variable environmental conditions in this upwelling area.     

厦门东海域定置网渔获鱼类种类组成及其季节变化

2003年7月～2004年6月对厦门东部海域的定置网渔获鱼类进行采样分析．结果表明厦门东海域至少有鱼类242种，隶属于19个目81科145属，主要是沿岸近底层和底栖的小型鱼类．以鲈形目种类最多，共计127种，占总数的52．5％、经济种类有116种．主要优势种有：青鳞小沙丁鱼、孔鳞小沙丁鱼、斑鲦、马拉邦虫鳗、裸鳍虫鳗、中华须鳗、前鳞鲻、眶棘双边鱼、多鳞鳝、皮氏叫姑鱼、短棘银鲈、列牙垒幸4、黄斑蓝子鱼、褐蓝子鱼、锯塘鳢、锻虎鱼类、褐菖鲇、绿鳍鱼、鳄鲡、卵鳎、中华单角鲍等．渔获种类在秋季及冬初最多，夏季较多，而冬末和春初最少．渔获种类的季节变化比较明显，与海洋表层水温变化有关，种类的月更替率比较高．