一种用磷酸钙法高效转染HEK 293T细胞方法的建立

利用磷酸钙转染法将lacZ-pShuttle质粒导入HEK 293T细胞中,实验证明,pH值、DNA纯度、转染后培养时间和甘油休克时间是影响转染效率的重要因素.转染后培养6h进行甘油休克效果最佳,比培养0h直接进行休克转染效率升高584.4%;甘油休克时间为4.5min时转染效果最佳,比不用甘油休克转染效率升高130.8%.通过对影响转染的几个条件进行优化,建立了1种可以高效转染HEK 293T细胞的方法,且简便易行.     

沉积物对磷酸盐的吸附与释放

本实验研究黄河口水文站沉积物对黄河水磷的吸附释放过程和东海１１１号站位沉积物对海水磷的吸附释放过程。并摸拟黄河沉积物在海水中磷的吸附释放过程。结果表明：底沉积物对磷的吸附相当迅速，无论是河泥还是海泥在２４ｈ内均能达到吸附平衡。吸附量与沉积物的量和水中原有磷的浓度有关，在不同的ｐＨ条件下，沉积物对磷的吸附是有显著差别的     

虾池的溶解氧含量及其补充量和消耗量

对对虾养殖池塘溶解氧含量及其收支情况的研究结果表明，虾池DO值具有明显的季节和昼夜变化。浮游植物光合作用是虾池溶解氧补充的主要来源，夏季最高可超过5mg／（dm3·d）。养殖后期对虾的耗氧量占总耗氧量的34％，底质和池水耗氧量分别占30％和35％。根据虾池氧的收支情况讨论了池养对虾的生产容量。     

Optical properties of low molecular weight and colloidal organic matter: Application of the ultrafiltration permeation model to DOM absorption and fluorescence

Cross-flow ultrafiltration (CFF) is often used to obtain separation and concentration of colloids from bulk natural water samples. Application of the ultrafiltration permeation model allows the quantitative determination of the low molecular weight material (LMW, < 1 kDa) and colloids in bulk dissolved organic matter (DOM) from measurements of time series permeate samples obtained from CFF. Detailed analysis of a Yukon River water sample shows that DOM absorption coefficient and fluorescence follow the permeation model and that the complex spectral optical properties of LMW DOM can be reconstructed from CFF data. A combination of measured and modeled data indicates that the LMW contribution to bulk DOM optical properties obtained from CFF can be grossly underestimated by the use of a low concentration factor (CF, the ratio of initial sample volume to retentate volume). Even at a relatively high CF of 19, optical properties of LMW DOM calculated from measurements of the retentate or integrated permeate would underestimate true values by 5–36%. In the Yukon River sample, LMW dissolved organic carbon represented 26% of the bulk concentration, but only 3–14% of the colored DOM was in the LMW fraction while 31–33% of bulk DOM florescence was due to LMW DOM. The contrasting optical properties of LMW and colloidal DOM support the concept that analysis of bulk DOM absorption and fluorescence properties reveals information about DOM molecular weight.     

台湾海峡上升流区夏季溶解氧的特征

本文根据1983—1984年,1987—1988年台湾海峡中北部海域调查资料,着重讨论了该海域夏季溶解氧分布特征及其与上升流、生物活动的关系。该上升流区温度、盐度、溶解氧含量及其饱和度的特征值分别为24.4℃,34.3,4.49mlO_2/L和93％。溶解氧含量及其饱和度与温度呈正相关关系,与盐度呈负相关关系。     

中国南极长城湾及附近海区1993/1994年夏季表层水样无机氮、磷酸盐的浓度及其分布状况

1993／1994年南极夏季对中国南极长城湾及附近海区表层海水四种营养盐浓度及其分布状况作了调查。结果表明，NH4－N、NO3－N、NO2－N及PO4－P的大致浓度分别是2．13、7．07、0．74和1．12μg·dm-3。一般来说，湾内高于湾外氮盐于12月份常出现高值。四种形态的无机盐类之最高、最低浓度出现的时间和站位不尽一致。∑N／P递减次序大致是12月，2月，1月。水温变化与NH4－N、PO4－P浓度和∑N／P的变化常较一致，而与NO2－N则常不一，一些站位上营养盐浓度偏低可能与浮游植物摄取有关。结果显示了调研海区的浅水内湾特征。浮游植物所需营养充分。该湾仍维持正常状态。     

珠江口水域化学耗氧量、溶解氧、无机磷与有机磷的三维水质数学模拟

珠江沿岸城市排污量大,对污染源治理能力十分有限,大量污染物通过河流或直接排放到珠江口,河口区半封闭的地形及潮水顶托使得污染物不易输送出外海,致使珠江口水质恶化,原有海洋生态环境遭到破坏,反过来制约了经济的发展.     

Nutrient budgets and trophic state in a hypersaline coastal lagoon: Lagoa de Araruama, Brazil

Lagoa de Araruama in the state of Rio de Janeiro, Brazil, is a hypersaline lagoon with salinity varying spatially from 45 to 56. We collected water samples during monthly cruises throughout the lagoon, and along the streams feeding the system, from April 1991 to March 1992. Nutrients and other water quality parameters exhibited great spatial and temporal variations. Mass balance calculations indicate large amounts of anthropogenic nutrient inputs. The data indicate that the lagoon currently is oligotrophic but is in a state of transition to become a mesotrophic system. Molar dissolved inorganic nitrogen:dissolved inorganic phosphorus (DIN/DIP) varied between 2.2:1 and 659:1 with a volume-weighted average of 22:1. The high DIN/DIP ratio contrasts with that found in nearby lagoons, suggesting that phytoplankton primary production is limited by phosphorus in Lagoa de Araruama. The major loss of DIP is apparently driven by biological assimilation and diagenic reactions in the sediments. Calculations indicate that the lagoon is slightly net autotrophic at +0.9 mol C m⁻² yr⁻¹. This suggests that the biomass of the primary producers is restricted by phosphorus availability. Phosphorus retention in the sediment and the hypersaline state of the lagoon prevent changes in autotrophic communities and the formation of eutrophic conditions.     

Origin and three-dimensional fluorescence spectrum of the chromophoric dissolved organic matter in Yangtze estuary, East China

Dissolved organic matter （DOM） is an important chemical component in natural water. Chromophoric dissolved organic matter （CDOM）, a fraction of optical properties, plays art important role in the biogeochemical cycle of nutrients in aquatic environment. People realized that DOM cycle is crucial in the global carbon and nitrogen flux, and also is inherently related to nutrients and trace metal elements. Therefore, CDOM was concerned by scientists in global oceanography and limnology fields. Water samples were collected from three sections （North Channel, South Channel and Zhuyuan） of the Yangtze （Changjiang River） estuary in March 2006 Three-dimensional excitation emission matrix （3-DEEM） fluorescence spectra were analyzed for those filtrates through Whatman GF/F filters. Dissolved organic carbon （DOC） was also measured by TOC analyzer. The tidal variety was also taken into account. The 3-D EEM fluorescence scans suggested the fluorescence characteristics of humic acid （Ex=332-344 nm, Em=439-451 nm） and fulvic acid （Ex=250-254 nm, Em=472-478 nm） were obvious, and the fluorescence group of protein-like and tyrosine （Ex=230 nm, Em=283 nm） was also found. They are mainly composed of CDOM in the Yangtze estuary. Further data analysis, especially the fluorescence index （f 450/500）, showed that terrestrial signal was rather strong （1.41-1.65） in the surface water, however, some terrestrial CDOM signals of bottom water showed excursions （1.28-1.39）. On the other hand, anthropogenic sign was impressed in the waters of Zhuyuan, which is one of the main drain outlets of Shanghai Metropolis. DOC concentrations ranged from 2.2 mg/L to 3.4 mg/L in Zhuyuan and South Channel, and from 2.0 mg/L to 2.4 mg/L in North Channel. The tide effect played a role in the composition of the CDOM measured by 3-D fluorescence scan technology.