Gene cloning and sequence analysis of the cold-adapted chaperones DnaK and DnaJ from deep-sea psychrotrophic bacterium Pseudoalteromonas sp.SM9913

Pseudoalteromonas sp.SM9913 is a phychrotrophic bacterium isolated from the deep-sea sediment.The genes encoding chaperones DnaJ and DnaK of P.sp.SM9913 were cloned by normal PCR and TAIL-PCR(GenBank accession Nos DQ640312,DQ504163).The chaperones DnaJ and DnaK from the strain SM9913 contain such conserved domains as those of many other bacteria,and show some cold-adapted characteristics in their structures when compared with those from psychro-,meso-and themophilic bacteria.It is indicated that chaperones DnaJ and DnaK of P.sp.SM9913 may be adapted to low temperature in deep-sea and function well in assisting folding,assembling and translocation of proteins at low temperature.This research lays a foundation for the further study on the cold-adapted mechanism of chaperones DnaJ and DnaK of cold-adapted microorganisms.     

Pressure Tight Piston Corer ——A New Approach on Gas Hydrate Investigation

A pressure tight sediment samphng technology, which can be introduced into the modification of the piston corer to accommedate the investigation of gas hydrate, is put forward. In this paper, the three basic rules of the technology are analyzed in detail: specimen transferring rule, seal self-tightening rule and semi-active pressure holding rule. Based on these, the structure of the pressure tight piston corer is put forward and its working principle is analyzed. Finally, a pressure tight sediment sampler, to which the same technology is apphed, is researched through experiments. Results show that the sampler based on the above-mentioned theory has a good ability in samphng and in -situ pressure holding.     

新世纪构造地质学的纵深发展:深海、深部、深空、深时四领域成就及关键技术

近10年来,构造地质学面临前所未有的机会和巨大的发展前景,建立超越板块构造理论的时代已经来临,地幔柱理论与板块构造理论的融合必将为太阳系乃至宇宙形成的构造过程提供全新认识。文中从宏观角度,综述了当前深部、深海、深空和深时4个发展方向取得的成就,展望了这4个方面的未来发展趋势。同时,综合概述了推动近10年来构造地质学发展的4个关键技术:针对表面构造形迹的高精度激光高度计(深空星球表面成像)、高分辨率多波束(深海海底地形成像);针对深部构造形态的层析成像(tomography);针对动力学演化(深时)的各种模拟软件(ANSYS、COMSOL、FLAC等);针对物质材料流变学的高温高压实验和成分原位测试技术。     

深海洋底热泉生态系和冷泉生物研究综述

现代海洋学、微生物学和生物学的研究表明，在深海火山活动和板块消减带所形成的地质背景下分别可能有海底热泉和冷泉的存在。在这种海底热泉和冷泉区的特殊环境中能够能海底热泉生态系的冷泉生物。它们能够依靠体内共生化学厌氧合成细菌产生营养，因而可能存在于完全缺氧的还原环境。海底热泉和冷泉生物学研究是对传统的生物学和地质学的挑战，人们还可能通过古代海底热泉和冷泉生态系的识别和分析，对涉及生命发生、生物演化、生物     

用数值模拟方法研究第四纪古气候──以LLN二维模式为例

本文着重介绍LLN模式对中晚第四纪大陆冰量变迁的模拟研究。由于天文学研究已经推算出大气层顶部太阳辐射过去和将来的长期变化，因而对本文的LLN二维模式而言，能否有效地进行古气候模拟在很大程度上取决于对古CO₂浓度的重建。随着对第四纪CO₂历史研究的不断深入，LLN模式对古气候长期变迁的模拟已从最初的末次冰期－间冰期延伸到0.6Ma B.P..。模拟的大陆冰量变化与地质记录对比良好，二者在与地球轨道要素有关的天文频率上的波动具有很高的相关性。     

基于长基线系统深海采矿ROV精确定位

对于深海采矿集矿机ROV的定位,采用传统的长基线水声定位系统通常存在较大误差。对此提出了一种新的算法模型,首先通过比较集矿机测量位置与前一次修正位置的几何关系,对当前集矿机的位置进行修正,然后利用新的集矿机位置修正声速的数值,并得出重新修正后的集矿机测量位置。这样反复的多重迭代使得在某一时刻集矿机ROV的测量位置逐步趋近其理想位置,实现长基线系统的水声精确的三维立体定位。通过数值仿真分析,结果显示与传统的长基线水声定位方法相比,通过该方法获得的集矿机行驶轨迹能更好地趋近其理想行驶曲线,表明该算法的有效性。     

Morphological description and population structure of an ophiuroid species from cobalt-rich crust seamounts in the Northwest Pacific: Implications for marine protection under deep-sea mining

Many seamounts are covered with cobalt-rich ferromanganese crusts, and are increasingly attracting interest for the potential extraction of valuable mineral resources from deep seabed environments. However, the impacts of potential mining activities on the vulnerable seamount ecosystem remain unclear. To enhance the understanding of connectivity in benthic invertebrate populations in the Northwest Pacific, several seamounts were surveyed and biological samples collected. In the present study, the ophiuroid species Ophioplinthaca defensor is reported for the first time from four deep seamounts in the Northwest Pacific, and described, providing more detailed morphological diagnosis characters. To assess the population structure of the species between and within seamounts, two mitochondrial markers (COI and 16S) were sequenced. In total, 20 haplotypes from 32 COI sequences and 8 haplotypes from 3716S sequences were recovered. The star-shaped TCS networks and non-significant pairwise population differences reveal the absence of distinct population structures between and within seamounts. In addition, the O. defensor population seemed to have undergone a demographic expansion in history. This is the first study on the genetic population structure of a benthic invertebrate from seamounts in the Northwest Pacific, and this results suggest a potentially high, long distance dispersal capacity in O. defensor between seamounts, which could inform the development of the Regional Environmental Management Plans for the cobalt-rich crust seamounts in the area.