Cryopreservation of Veliger Larvae of Trumpet Shell,Charonia sauliae: an Essential Preparation to Artificial Propagation

Trumpet shell, Charonia sauliae, is an endangered and valuable species, but its artificial propagation protocol has not been successfully established. To estimate the possibility of cryopreservation for larvae of C. sauliae, which is a potential preparation for its artificial reproduction and further research, in this study a protocol for the cryopreservation of veliger larvae of trumpet shell was optimized. Through a two-step cryopreservation procedure, four kinds of cryoprotectants (ethylene glycol, 1, 2-propanediol,dimethyl sulfoxide and glycerol) were employed at three concentrations (1.0, 1.5 and 2.0 molL-1) respectively and survival rates of larvae were determined after a storage of 1h. The larvae frozen with these four cryoprotectants after 1h storage were cultured, and then survival rates were determined at 24, 72 and 120h after thawing. Dimethyl sulfoxide at a concentration of 1.5 molL-1 showed the best protective effect in all experiments (p<0.05). And survival rates of larvae frozen with dimethyl sulfoxide were determined after 1,7 and 15 d of storage. The survival rates of larvae frozen with 1.5 molL-1 dimethyl sulfoxide after 1h, 1d, 7 d and 15 d of storage were 80.77% ±7.51%, 80.34% ±11.28%, 83.10% ±9.14% and 77.23%±6.22% respectively. No significant differences in survival rates of larvae frozen with dimethyl sulfoxide were observed after various storage periods (p>0.05).     

Hydrodynamic performance of a dual cylindrical caisson breakwater

The hydrodynamic performance of a dual cylindrical caisson breakwater (DCBW) formed by a row of caissons each of which consisting of a porous outer cylinder circumscribing an impermeable inner cylinder has been theoretically investigated. The theoretical formulation is based on the eigenfunction expansion method proposed by Spring and Monkmeyer (1974) which was further modified by Linton and Evans [Linton, C.M., Evans, D.V., 1990. The interaction of waves with arrays of vertical circular cylinders. Journal of Fluid Mechanics 215, 549–569] for an array of impermeable cylinders. The present formulation is an extension of the work of Wang and Ren [Wang, K.H., Ren, X., 1994. Wave interaction with a concentric porous cylinder system. Ocean Engineering 21(4), 343–360], wherein; the interaction of linear waves with a single concentric porous cylinder system was studied. In the present study, the formulation has been extended to the case of a group of porous dual cylinder system. Parametric studies are carried out to study the influence of porosity (G₀) on the outer caisson, width of the doughnut chamber (a/b) and the angle of wave incidence on the variation in the hydrodynamic loading, wave run-up, free-surface elevation in its vicinity as well as the transmission on its lee-side. The importance of the presence of the inner cylinder in achieving the required hydrodynamic performance in terms of either protection or providing tranquility on its lee side keeping higher stability for the breakwater system is highlighted.     

Numerical Simulation of Large Diameter Cylindrical Structure Slamming

The water entry of large diameter cylindrical structure is studied by applying numerical simulation method. The processes of different diameter cyhndrical structures impacting water with various constant velocities are calculated numerically. Thereafter, analyzed are the distribution of slamming pressure on structure during slamming course and the influence of slamming velocity and cylindrical diameter on slamming process. Furthermore, presented herein is an equation being used to forecast the peak slamming force on a large diameter cylindrical structure.     

Biometric,microstructural, and high-resolution trace element studies in Crassostrea gigas of Cantabria (Bay of Biscay,Spain): Anthropogenic and seasonal influences

Living Crassostrea gigas oysters of different ages and sizes were collected in three estuaries of Cantabria (Bay of Biscay, Spain): San Vicente de la Barquera Estuary, Santander Bay, and Marismas de Santoña Estuary. The main objective was to determine different shell responses to variable environmental parameters. A shell morphological study, based on three biometric indices, indicates that oysters of Santander Bay have two significant shell anomalies: abnormal thickening of the right valve and loss of vital cavity volume. These shell abnormalities are related with the presence in these waters of the chemical tributyltin. In the other two estuaries, the oysters show no detectable anomalies. Four shell microstructures have been distinguished: Regular Simple Prismatic, Regular Foliated, cone-Complex Cross Foliated, and Chalk. In Santander Bay oysters, the Chalk forms a “root-type” framework, whereas in the other two estuaries it forms a more compact microstructure. It is proposed that exposure to tributyltin has produced this modification. High-spatial-resolution geochemical transects have been carried out on the Regular Foliated microstructure in the umbo region in order to evaluate the distribution of Mg, Sr, and Na. The elements analysed exhibit clear cyclic variations in San Vicente de la Barquera Estuary and Marismas de Santoña Estuary oysters, related with seasonal periods, and characterised by broad maxima during months in which the waters are warmer and have higher salinity. These patterns are buffered in Santander Bay oysters. Our results demonstrate that biometric, microstructural, and high-resolution trace element studies in oyster shells can provide information about contaminants and seasonal variations in the estuarine environment.     

Effects of parasitism and environment on shell size of the South American intertidal mud snail Heleobia australis (Gastropoda)

The aim of this work was to evaluate the effects of parasitism and certain environmental factors on the shell size of Heleobia australis (Hydrobiidae, Cochliopinae). We report sporocysts and metacercariae of Microphallus simillimus (Microphallidae, Trematoda) parasitizing the gonad and digestive gland of H. australis specimens from two sites of Bahía Blanca estuary, Argentina. The prevalence of infection was significantly higher (34.17% in winter and 68.14% in late spring) in snails from the outer estuary at Site 2 than in those from the inner estuary at Site 1 (5.88% and 4.71% respectively). The only known definitive host for this digenean is the white-backed stilt Himantopus melanurus (Recurvirostridae, Aves), most abundant in the estuary during winter. Parasitism by M. simillimus causes variations in the shell dimensions of H. australis, the shells of infected snails being narrower than those of uninfected snails. Snails from Site 2 were found in general to be significantly smaller than those at Site 1, possibly as a result of differences in environmental factors such as the degree of exposure to wave energy, the allocation of energy to reproduction rather than growth (induced by predation and/or parasitic castrators) and anthropogenic stressors.     

从贝丘遗址看绳纹人与环境的相互关系

介绍了自本世纪７０年代末以来，日本学者在绳纹时代的考古研究中开辟的一个新领域，即通过分析贝丘遗址和周围的自然环境去认识绳纹的环境变化及绳纹人与环境的关系。同时提出了笔者关于绳纹时代人与环境关系的一个模式。     

利用贝类(Lamprotula)化石碳氧同位素再建山西丁村地区晚更新世气候环境

较大的湖泊中的贝类生长线的氧同位素可能记录了季节性的温度变化。对拔氏丽蚌 (楔丽蚌 )Lamprotula(Cuneolamprotula)bazini (Heude)和环带丽蚌 (丽蚌 )Lamprotula (Lamprotula)zonata (Heude)的生长线进行氧同位素研究表明,前者有较强的适应环境变化的能力。δ18O值记录的襄汾地区晚更新世的古温度为 2 0～ 2 4℃至 30～34℃,古降雨量多出现代 2 0 0～ 2 6 7mm,河水排泄不畅,积水成湖。这些结果给人的启示是:全球变暖后,该地区气温可能有较大升幅 ;降雨量的增加有可能在排水不畅地区形成水灾,应注意防患于未然。     

自聚焦光纤中传导模的场分布

从亥姆霍兹方程出发，导出了自聚焦光纤中传导模在圆柱坐标系中的场分布形式，并给出了几个低阶模的分布图。     

鳖甲溃疡和穿孔病的病原及其防治

对鳖甲溃疡和穿孔病的病原进行了研究,提出了勒氏假单胞菌（Pseudomonaslemoignei）、产碱菌（Alcaligenessp）和枯草芽孢杆菌（Bacillussubtilis）3种病原菌;同时在病原药敏试验的基础上筛选治疗药物（鳖康乐1号）,并进行了生产性防治试验。     

粉石英在蓄电池胶壳中的应用

本文着重介绍了粉石英的工艺矿物特性以及其在蓄电池胶壳中的应用效果,证明了粉石英用作蓄电池胶壳的填料,能够改善混炼工艺、提高产品质量、增加填充量、降低生产成本,是一种理想的橡胶填料。