一株海洋几丁质酶产生菌的筛选及其产酶条件的初步研究

从胶州湾的海泥中分离到一株几丁质酶高产菌株。经16SrDNA序列分析,认为该菌属于假交替单胞菌(Pseudoaltermonas),并将其命名为SS01。对该菌产几丁质酶的最佳培养条件进行了研究,发现与其它几丁质酶产生菌相比,SS01菌株的最适产酶温度较低,酶活较高。在Zobell2216E培养基中,SS01菌株培养9h进入稳定期。胶体几丁质可诱导该菌几丁质酶的产生。在几丁质培养基中,SS01菌株产酶的适宜条件是:培养温度24℃,培养基起始pH7.0左右,蛋白胨为N源(5g/L),胶体几丁质为C源(5g/L),170r/min振荡培养130h,酶活可达116.2U/mL。     

Phenology ofMedicago minimaandErodium cicutariumin semi-arid Argentina

The phenology ofMedicago minimavar.minimaandErodium cicutariumwas studied at two different field sites in the Calden District (approx. 10 million ha), a temperate semi-arid phytogeographical region in central Argentina. One site had been protected from wild and domestic herbivores for the 6 previous years. The other adjacent site had been exposed to continuous grazing by cattle for several decades. A phenological key was made for each species in 1988, and this key was improved during the 1989 growing season when phenological observations were made every 1–3 weeks.The growth cycle ofM. minimaandE. cicutariumwas similar at the two studied sites. Initiation of the cycle occurred in autumn and appeared to be associated with soil water availability. Phenological patterns were very dynamic at the reproductive stage in both species. This stage started earlier and was faster inE. cicutariumthan inM. minima. The end of the growing season occurred during late spring, concomitant with high maximum air temperatures. Our results suggest that these species hasten their development when air temperature increases and soil water availability decreases. This could be an important strategy in these species which allows them to persist as seeds, and produce a new generation under favourable environmental conditions.     

Review on Vegetation Phenology Observation and Phenological Index Extraction

Vegetation phenology directly reflects the response of ecosystem physiological and ecological processes to environmental changes and has received increasing attention in climate change, which leads to the development of various phenology monitoring techniques and methods of extracting the phenological index. Based on relevant literatures, the monitoring techniques of vegetation phenology were reviewed. Second, the methods of extracting phenological index using different techniques were introduced systematically. Thirdly, the differences from multi-source data and from multi-methods were evaluated based on ground phenology record, flux observation and vegetation index. Although various methods have been adopted in current vegetation phenological studies, it should be aware of their appropriately spatiotemporal scales for different methods, and the mutual complementation will help to improve the system for phenology monitoring. Meanwhile, the comparison indicated the obvious differences in derived vegetation phenological index using various observation techniques and extraction methods, especially for the autumn vegetation phenological index. Such results highlight the importance to assess reasonably these differences from multi-source data and from multi-methods, and to establish a rational processing system for the mutual comparison and conversion from different methods.     

植物物候遥感监测研究进展

基于遥感监测方法的植物物候研究已成为全球气候变化研究的一个热点课题,通过对植物物候生长季概念的遥感界定,归纳总结了物候监测的基本原理、遥感数据源以及4种常用的遥感监测方法,并指出各种遥感监测方法存在的问题和不足;以遥感监测技术为支撑,对地区、大洲和全球尺度上物候与气候关系的研究、物候与人类行为关系的研究进行了阐述,指出植物物候的变化常常是二者共同影响的结果。针对当前物候研究中存在的不足,探讨了今后的研究发展方向。     

Analysis of differences in vegetation phenology cycle of abandoned farmland,using harmonic analysis of time-series vegetation indices data: the case of Gwangyang City,South Korea

ABSTRACT

Globally, countries have experienced substantial increases in farmland abandonment. Although vegetation phenology is a key factor for the classification of land use, understanding of the phenological change of abandoned farmland is lacking. Using harmonic analysis of NDVI and NDWI extracted from Landsat imagery, this study investigates the distinctive phenological characteristics of abandoned farmland, which contrasts with that of three other agricultural types (paddy, agricultural field, orchard) in the study site of Gwangyang City in Jeollanam Province, South Korea. The results suggest that abandoned farmland has higher overall greenness coverage and overall water content in vegetation than the other uses. In terms of both indices, abandoned farmlands changed with relatively less fluctuation than those of other uses, suggesting the existence of constant and unmanaged vegetation from ecological succession, which differs from crop fields that undergo cultivation procedures. The significant harmonic components differed among agricultural types and vegetation indices. In paddy, NDVI was explained with multiple, higher-order harmonic components, while in other types only first-order components met the 5% statistical significance level. With NDWI, land types were more clearly discernible, because of the different cultivation procedures involving water: wet-field method (paddy), dryland farming (orchard, agricultural field), and no cultivation (abandoned farmland). The analysis confirms that harmonic analysis could be useful in discerning abandoned farmland among areas of active agricultural use and shows that the statistical significance of harmonic terms can be employed as indicators of different agricultural types. The observed pattern of the geographic distribution of abandoned farmland has policy implications for the promotion of sustainable reuse of marginal farmland.     

基于翻转课堂的三本院校应用型课程教学改革模式的研究

笔者所在院校为地处赣南老区的三本学校,教学中存在师资缺乏、软硬件设施不足、资源共享滞后等问题,严重制约了学院人才培养和专业发展。随着MOOC教学的不断深入和发展,为学院的教学改革、人才培养和师资队伍建设提供了一个新的教学方向。为进一步提高笔者所在院校人才培养质量,培养出适应于社会发展所需的应用型专业技术人才,笔者根据多年的教学工作经验,从学生、教师和学校3个层面,分析并指出了三本院校学生及教师教学中存在的一些问题,设计了基于MOOC平台的教学改革设计方案,提出了教学改革的研究方法和内容,以期为三本院校及应用型本科院校人才培养和课程教学改革提供新思路和新方法。     

黑河流域遥感物候产品验证与分析

植被物候遥感产品对全球变化响应、农业生产管理、生态学的应用等多领域研究具有重要意义。但现有植被物候遥感产品还有较多问题,主要包括一方面使用不同参数的时间序列数据以及不同提取算法导致的产品结果差异较大,另一方面在地面验证中地面观测数据与遥感反演数据的物理含义不一致导致的验证方法的系统性误差。本文以黑河流域为研究区,对比验证基于EVI(Enhanced Vegetation Index)时间序列数据提取的MLCD(MODIS global land cover dynamics product)植被遥感物候产品和基于LAI(Leaf Area Index)时间序列数据提取的UMPM(product by universal multi-life-cycle phenology monitoring method)植被遥感物候产品的有效性及精度等。同时,通过验证分析进一步评估基于EVI和LAI时间序列提取的物候特征的差异及特点,探讨由于地面观测植被物候与遥感提取植被物候的物理意义的不一致问题导致的直接验证结果偏差。结果表明:UMPM产品有效性整体高于MLCD产品,但在以草地和灌木为主的稀疏植被区,由于LAI取值精度的原因,UMPM产品存在较多缺失数据,且时空稳定性较低;基于玉米地面观测数据表明,EVI对植被开始生长的信号比LAI更加敏感,更适合提取生长起点,但植被指数易饱和,峰值起点普遍提前,基于LAI提取的峰值起点更加合理。由于地面观测的物候期在后期更加关注果实生长,遥感观测仅关注叶片的生长,遥感定义的峰值终点和生长终点与玉米的乳熟期和成熟期差异较大。     

模糊综合评判在土地适宜性评价中应用研究

在十堰市土地利用现状调查的基础上,针对现有坡荒地,通过对制约土地的自然因素和社会经济条件的综合分析,依照土地质量满足对预定用途要求的程度,采用模糊数学的方法,在计算机上完成了坡荒地的宜农、宜林、宜牧、宜园4个适宜类的评价;同时对宜农地进行了更进一步的讨论。     

Comparison of eMODIS and MOD/MYD13A2 NDVI products during 2012–2014 spring green-up periods in Alaska and northwest Canada

Accurate monitoring of vegetation dynamics is required to understand the inter-annual variability and long term trends in terrestrial carbon exchange in tundra and boreal ecoregions. In western North America, two Normalized Vegetation Index (NDVI) products based on spectral reflectance data from the Moderate Resolution Imaging Spectroradiometer (MODIS) are available. The MOD/MYD13A2 NDVI product is available as a 16-day composite product in a sinusoidal projection as global hdf tiles. The eMODIS Alaska NDVI product is available as a 7-day composite geotif product in a regional equal area conic projection covering Alaska and the entire Yukon River Basin. These two NDVI products were compared for the 2012–2014 late May–late June spring green-up periods in Alaska and the Yukon Territory. Relative to the MOD/MYD13A2 NDVI product, it is likely that the eMODIS NDVI product contained more cloud-contaminated NDVI values. For example, the MOD/MYD13A2 product flagged substantially fewer pixels as “good quality” in each 16-day composite period compared to the corresponding MODIS Alaska NDVI product from a 7-day composite period. During the spring green-up period, when field-based NDVI increases, the eMODIS NDVI product averaged 43 percent of pixels that declined by at least 0.05 NDVI between 2 composite periods, consistent with cloud-contamination problems, while the MOD/MYD13A2 NDVI averaged only 6 percent of pixels. Based on a cloudy Landsat-8 scene, the eMODIS compositing process selected 23 percent pixels, while the MOD/MYD13A2 compositing process selected less than 0.003 percent pixels. Based on the results, it appears that the MOD/MYD13A2 NDVI product is superior for scientific applications based on NDVI phenology in the tundra and boreal regions of northwestern North America.     

潮汐和植被物候影响下的潮间带湿地遥感提取

潮间带湿地具有重要的生态和经济价值,但受到全球变化影响,发生大面积退化甚至丧失.掌握潮间带湿地的时空分布特征,对海岸带资源的科学管理具有重要意义.由于受到多云多雨天气和潮汐动态淹没的影响,单时相遥感数据难以获取完整的潮间带湿地信息.因此,本研究开发了一种基于时序遥感指数的潮间带湿地分类算法,并以福建省亚热带海岸带为例,...