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21.
A strain of yeast, which can endure high osmotic pressure, is employed for the sensitive material of the microbial BOD sensor. Two immobilization methods are used, I.e. Calcium alginate gel be ads and PV A gel beads. The results show that the PVA gel beads is better. The influences of osmosis and heavy metal ions on the yeast entrapped in the PVA gel beads are also studied in the experiment. 相似文献
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凡纳滨对虾咸淡水养殖系统内细菌群落组成的PCR-DGGE分析 总被引:16,自引:0,他引:16
采用PCR(polym erase chain reaction)-DGGE(denaturing grad ient gel electrophoresis)及传统的微生物培养方法对凡纳滨对虾Litopenaeus vannam ei咸淡水养殖系统内各种环境的细菌群落组成进行了比较研究。传统的微生物培养计数表明,从海湾水、养殖池水到对虾粪样,细菌和弧菌的数量表现出依次增加的趋势,粪样及肠壁中弧菌的数量高出外界水环境1—4个数量级。相对于外界水环境,粪样中有很高的芽孢杆菌孢子含量,但是肠壁定植细菌中不存在芽孢杆菌(孢子)。PCR-DGGE及聚类分析结果表明,从海湾沿岸、养殖池、对虾粪便到对虾肠壁,细菌群落的多样性由高到低,无论是在哪种环境,群落的优势种都十分明显,且只有2—4种。来自同一环境各样品间的细菌群落组成非常相似,来自不同环境的样品,其细菌群落组成差别较大。聚类图上各簇的排列顺序反映了样品在取样空间分布上的毗邻次序和它们的相似程度。 相似文献
25.
Microbial Biomass Dynamics Along a Trophic Gradient at the Atlantic Barrier Reef off Belize (Central America) 总被引:2,自引:0,他引:2
Abstract. Recent findings indicate that heterotrophic bacteria and not phytoplankton are the most numerous biomass components even in the euphotic zone of oligotrophic, open oceans. In this study it was hypothesized that the microbial biomass components change within a few hundred meters as oligotrophic water flows across the reef and becomes enriched with nutrients. Along a trophic gradient, four stations at the Atlantic Barrier Reef off Belize (Central America) were sampled for microbial biomass components. Phytoplankton biomass (measured as chlorophyll a) ranged from the most oligotrophic station (St. 1) to the most eutrophic station (St. 4) from 6.9–415.5 μg CI"' (assuming a C:chl a ratio of 30): heterotrophic bacterial biomass increased 4-fold (from 10.1–46.4μg C 1-1 ), heterotrophic nanoflagellate (HNAN) biomass increased from 4.6-19ug C 1-1 , and cyanobacteria from 0.9-4.5 μg C-1-1 . Production estimates derived from seawater cultures revealed a 5-fold increase in bacterial production from the oligotrophic station (3.7 ug C 1-1 d-1 ) to the eutrophic St. 4 (17.8ug C-1-d1-1 )- Cyanobacterial production rose from 1.1–3.5ug C-1–d-1 and HNAN production from 0.65-1.13 μg C-1-1 -d-1 . While cyanobacteria contributed between 13 and 20% to the autotrophic plankton component in the oligotrophic waters, their contribution dropped to about 1 % at the eutrophic stations. 相似文献
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海洋木栖真菌抗菌活性的初步研究 总被引:6,自引:0,他引:6
从福建厦门海沧、集美及漳州浮宫等地采集到的红树林等海洋潮间带的各种腐木样品中分离得到176株海洋木栖真菌,对其进行抗菌活性检测.其结果表明共有96株海洋木栖真菌对大肠杆菌、枯草杆菌及白色假丝酵母中的一种或多种拮抗指示菌具有抑制作用,占供测菌株总数的54.5%.在这些活性菌株中,红树源菌株拮抗比例为30.2%,非红树源菌株拮抗比例为69.8%.具有抗菌活性的菌株主要分布于15个属中,包括木霉Trichoderma、青霉Penicillium、拟青霉Paecilomyces和一些不产孢的分类群.某些稀有真菌如盘多毛孢Pestalotia、茎点霉Phoma也具有一定的抗菌活性. 相似文献
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Natalia V. Zhukova 《Ocean Science Journal》2005,40(3):34-42
Variation in the microbial biomass and community structure found in sediment of heavily polluted bays and the adjacent unpolluted
areas were examined using phospholipid fatty acid analysis. Total microbial biomass and microbial community structure were
responding to environmental determinants, sediment grain size, depth of sediment, and pollution due to petroleum hydrocarbons.
The marker fatty acids of microeukaryotes and prokaryotes - aerobic, anaerobic, and sulfate-reducing bacteria -were detected
in sediments of the areas studied. Analysis of the fatty acid profiles revealed wide variations in the community structure
in sediments, depending on the extent of pollution, sediment depth, and sediment grain size. The abundance of specific bacterial
fatty acids points to the dominance of prokaryotic organisms, whose composition differed among the stations. Fatty acid distributions
in sediments suggest the high contribution of aerobic bacteria. Sediments of polluted sites were significantly enriched with
anaerobic bacteria in comparison with clean areas. The contribution of this bacterial group increased with the depth of sediments.
Anaerobic bacteria were predominantly present in muddy sediments, as evidenced from the fatty acid profiles. Relatively high
concentrations of marker fatty acids of sulfate-reducing bacteria were associated with organic pollution in this site. Specific
fatty acids of microeukaryotes were more abundant in surface sediments than in deeper sediment layers. Among the microeukaryotes,
diatoms were an important component. Significant amounts of bacterial biomass, the predominance of bacterial biomarker fatty
acids with abundance of anaerobic and sulfate-reducing bacteria are indicative of a prokaryotic consortium responsive to organic
pollution. 相似文献
30.
Ana P. Mucha M. Teresa S. D. Vasconcelos Adriano A. Bordalo 《Estuarine, Coastal and Shelf Science》2004,59(4):663
Vertical distribution (0–15 cm) of the macrobenthic community and its relationships to natural sediment characteristics and trace metal contents and bioavailability were studied at five locations in the lower Douro estuary, Portugal. An analysis of vertical metal distribution, for the interpretation of anthropogenic impact on the estuarine sediments, was also investigated. Sediment characterisation included organic matter, grain size, metals (Al, Fe, Cu, Pb, Cr, Ni, Cd, Zn and Mn), acid volatile sulphide (AVS) and simultaneously extracted metals (SEM). The macrobenthic community had low diversity (14 species), was dominated by small size opportunists and seemed to be controlled mainly by natural factors such as grain size distribution, Al and Fe contents and sediment depth. The vertically heterogeneous distribution of macrobenthic community appears to affect redox status of the sediments and consequently metal bioavailability. Despite anthropogenic contamination in terms of Zn, Cu, Pb, Cr and Ni having already been detected in the north bank, the analysis of vertical distribution was essential for the identification of current anthropogenic contamination in terms of Zn, Pb and Cd in the south bank. 相似文献