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从近江牡蛎肠道中分离出21株菌,其中, 9株为革兰阳性菌, 12株革兰阴性菌。为寻找能分泌多种酶的有益微生物,研究了它们蛋白酶、脂肪酶、淀粉酶、纤维素酶的产酶能力。结果表明,有61 .9%的菌株能分泌蛋白酶或淀粉酶(各有13株菌);有52. 4%的菌株能分泌脂肪酶(11株菌);有33. 3%的菌株能产纤维素酶(7株菌)。在这21株菌中,产4种酶的有5株菌,产3种酶的3株菌,产两种酶的5株菌,产1种酶的3株菌,不产酶的仅有5株菌。不产酶的菌株仅占细菌总数的23. 8%,而各种产酶菌则高达76. 2%,由此表明细菌在近江牡蛎对食饵消化过程的重要性。 相似文献
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Nodularia spumigena periodically proliferates to cause toxic algal blooms with some aquatic animals enduring and consuming high densities of the blue green algae or toxic lysis. N. spumigena contains toxic compounds such as nodularin and lipopolysaccharides. This current work investigates physiological effects of exposure from bloom conditions of N. spumigena cells and a post-bloom lysis. Biochemical and antioxidative biomarkers were comparatively studied over an acute 3-day exposure. In general, a post-bloom N. spumigena lysis caused opposite physiological responses to bloom densities of N. spumigena. Specifically, increases in glutathione (GSH) and glutathione peroxidase (GPx) and decreases in glutathione S-transferase (GST) were observed from the N. spumigena lysis. In contrast, N. spumigena cell densities decreased GSH and increased GST and lipid peroxidation (LPO) in mussels. Findings also suggest that at different stages of a toxic bloom, exposure may result in toxic stress to specific organs in the mussel. 相似文献
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Comparison of two methods for ground level vapour sampling and influence of meteorological parameters on its stable isotopic composition at Roorkee,India
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Ground level vapour (GLV) samples were collected at Roorkee, Uttarakhand, India using two methods: liquid condensation (LC) at 0 °C and cryogenic trap (CT) at ?78 °C for the period 2009–2011. The study reveals that there is a considerable fluctuation in stable isotopic composition of GLV throughout the year. The study area receives complex moisture source during different seasons, which is evident from the moisture flux received during different seasons. The isotopic composition of the GLV in both methods shows depleted nature during rainout process. CT method shows exact isotopic signature of GLV because of maximum trapping of air moisture and its condensation, whereas LC method shows depleted or enriched character because of the prevalence of kinetic and diffusive fractionation. The d value shows that LC method acts as magnifier of the CT method and clearly shows seasonal effect than the clustered CT method. Hence, to decipher the original isotopic signature of GLV, isotopic composition of GLVLC can be converted to GLVCT by deriving an empirical relationship with changing season and locations. Meteorological parameters show varied behaviour with GLVCT and LC because of moisture sources in all seasons. The GLVCT and LC method shows significant correlation with meteorological parameters when the region is dominated by single moisture source. The GLVLC method magnifies the correlation with meteorological parameters when the region is influenced by more than one source. The study shows that the GLVLC methods can be used in place of GLVCT when the objective is to understand the influence of different moisture sources on GLV. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
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以初始体重(1.08±0.04)g的云斑尖塘鳢(Oxyeleotris marmoratus)幼鱼为实验对象,进行为期8周的摄食生长实验,研究不同饵料(虾肉、鱼肉、杂色蛤肉和水蚯蚓)对云斑尖塘鳢幼鱼的摄食生长、体成分和消化酶活性的影响。每个处理设4个重复,每个缸内随机放养20尾幼鱼。结果表明:水蚯蚓组的增重率、特定生长率、饵料效率和蛋白质效率均显著高于其他饵料组(P0.05),鱼肉组显著低于其他饵料组(P0.05)。水蚯蚓组的粗蛋白含量显著高于其他饵料组,而鱼肉组最低,虾肉组和杂色蛤肉组差异不显著(P0.05),水蚯蚓组的粗脂肪含量显著高于其他饵料组(P0.05),但灰分含量显著低于其他饵料组(P0.05);胃蛋白酶活力为水蚯蚓组虾肉组和杂色蛤肉组鱼肉组(P0.05),各组的脂肪酶和淀粉酶活力差异不大(P0.05)。研究表明,水蚯蚓是云斑尖塘鳢幼鱼的最佳饵料,而实际生产中最常使用的鱼肉效果最差。 相似文献
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不同蛋白水平饲料对刺参生长和消化酶活性的影响 总被引:1,自引:0,他引:1
通过在刺参饲料中添加不同含量的鱼粉,调节饲料中蛋白水平,测定不同蛋白水平对刺参(Apostichopus japonicus)消化酶活性的影响,以稳定同位素分析技术研究刺参对不同食物组分的吸收效率。实验分6个饲料处理组,鱼粉添加量分别为0(A组)、2%(B组)、5%(C组)、10%(D组)、15%(E组)和20%(F组)。实验过程中,分别于初始时间、第一周、第二周、第四周、第八周和第十二周采集刺参样品,测定刺参的特定生长率、稳定同位素13 C/12 C、15 N/14 N值和消化酶活性。结果显示:D组刺参的特定生长率最高,且与C组无显著差异(P0.05),F组显著低于其它组(P0.05);D组鱼粉对刺参的食物贡献率最高,显著高于其它组(P0.05);消化酶活性方面,蛋白酶和淀粉酶活性均为C组最高,且在饲养过程中,蛋白酶活性一直呈上升趋势,而淀粉酶活性则是先下降后上升。从刺参生长、鱼粉食物贡献率和消化酶活性综合考虑,鱼粉添加量在5%~10%最利于刺参生长。 相似文献
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The effects of a diet containing Hanseniaspora opuntiae C21 on growth and digestive enzyme activity were estimated in juvenile Apostichopus japonicus. Groups of sea cucumbers were fed diets containing H. opuntiae C21 at 0(control), 10 4, 10 5, and 10 6 CFU(colony-forming units)/g feed. Results showed that after 45 d the specific growth rate(SGR) of sea cucumbers fed a C21-supplemented diet at 10 4 CFU/g feed was significantly higher than that of the control( P 0.05). Intestinal trypsin and lipase activities were significantly enhanced by C21 administration at 10 4 and 10 5 CFU/g feed compared with the control( P 0.05). After feeding for 23–42 d, C21 was demonstrated by denaturing gradient gel electrophoresis to be present in the intestine of sea cucumbers. In addition, after feeding the C21-supplemented diets for 15 d, the sea cucumbers were switched to an unsupplemented diet and C21 was confirmed to be capable of colonizing the intestine for at least 31 d after cessation of feeding. In conclusion, C21 was shown to successfully colonize the intestine of juvenile A. japonicus via dietary supplementation, and improve growth and digestive enzyme activity. 相似文献
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