基于精细数据处理的叠前深度偏移在琼东南盆地陵水凹陷坡折区的应用

琼东南盆地陵水凹陷,受坡折区崎岖海底和复杂地质构造的影响,导致该地区速度模型构建精度不高,从而严重影响到成像品质。针对坡折区的精确速度模型构建难题,首先采用组合多次波衰减、宽频处理、叠前信噪比增强等针对性处理技术,得到宽频带高信噪比的叠前数据,在此基础上统计分析不同水深速度规律,应用断控和地质约束速度建模技术,建立初始速度模型,然后利用基于宽频数据的高分辨断控约束网格层析反演技术,经过多次迭代完成深度域各向异性速度建模,并实现了陵水凹陷坡折区的高精度叠前深度偏移成像。叠前深度偏移成像显示,基于精细速度模型的叠前深度偏移技术科明显提高坡折区信噪比,改善成像质量,同时可有效消除地层同相轴扭曲的现象,恢复了地下地层的真实构造形态,从而可为目标评价与钻探决策提供更可靠地震资料。     

栉孔扇贝精巢组织细胞的体外培养及特征分析

本研究建立了一个栉孔扇贝精巢组织细胞的原代培养体系,鉴定了原代细胞的功能基因表达特征。结果显示,原代培养体系中的精巢细胞以圆形细胞为主,直径为4~11μm,其在体外已存活9个月以上。使用栉孔扇贝vasa基因(生殖细胞标记基因)的地高辛RNA探针和原位杂交技术检测,约98%的原代细胞呈阳性。利用免疫组化技术鉴定了栉孔扇贝SCP3、KLF4、PIWI、C-MYC、SOX9和SOX17在这些细胞中的表达特征,发现这些靶蛋白在精巢原代细胞中的表达特征与栉孔扇贝精巢中的细胞定位基本一致。上述结果表明,本实验所获得的原代细胞以生殖细胞为主,它们保留了栉孔扇贝雄性生殖细胞原有的基因表达特征,可以用于贝类精子发生和性别相关的功能基因研究。     

费尔干纳盆地油气成藏特征及其主控因素分析

费尔干纳盆地是位于中亚地区南天山山系中呈负向地形的坳陷,受基底断裂和区域构造活动控制,形成3个不同的构造层系。盆地基底为褶皱造山条件下形成的强烈变质的古生代地层,中部的盆地盖层沉积为中生代至古近纪拉张裂谷稳定沉降条件下形成的,最上部为新近纪至现今造山阶段形成的磨拉石建造。费尔干纳盆地主要发育中下侏罗统湖相沼泽相泥页岩、上白垩统乌斯特里奇组区域海侵层和古近系海相泥岩和泥灰岩等3套烃源岩;发育侏罗系、白垩系、古近系及新近系等4套含油气储集层系;且区域性盖层、准区域性盖层和局部盖层发育,形成了良好的生储盖组合。受构造变形和沉积环境控制,主要发育构造型、构造—地层型和地层(岩性)型3大类型油气藏(田)。第四纪早期的构造活动控制了盆地内油气田的最终分布,扭断层破坏了已有的圈闭并为烃类向上覆新近系储层的运移提供了运移通道,造成大量烃类气体散失。     

空间互相关方法在分析海底沙波迁移规律中的应用

海底沙波具有显著的活动性并能够对海底工程设施造成潜在威胁,因此对海底沙波活动性的评估一直以来受到广泛关注。目前大多数研究者仍利用平面剖面对比来分析海底沙波的迁移特征。然而,该方法难以全面高效地获取海底沙波二维平面迁移矢量。本文基于2007年和2009年的高分辨率多波束测深数据,详细阐述了空间互相关方法在分析北部湾东南海域海底沙波迁移规律中的应用过程。利用实测数据进行对比分析,获得了研究区海底沙波的活动特征,并进一步讨论了空间互相关算法中不同参数的选取对结果的影响。结果显示,利用空间互相关分析方法能够有效获取海底沙波二维迁移矢量,获得的海底沙波迁移速率和迁移方向与前人研究成果吻合,表明了该方法的可行性和可靠性。但在对实际DTM数据进行空间互相关分析时,需根据海底沙波形态、数据质量等因素选取合适的参数及矢量获取方法。本研究实验了一种确定海底沙波迁移规律的新方法,该方法将有效提升获得海底地形变化规律的效率和准确度,可获得更加精细的海底地形动力过程。     

浮游植物metacaspase的分布、结构及其功能特性

精氨酸/赖氨酸特异性半胱氨酸蛋白酶(Metacaspase)在浮游植物程序性细胞死亡(programmed cell death.PCD)过程中起着关键作用。本文利用生物信息学方法分析metacaspase基因在浮游植物中的分布,并以海洋球石藻(Emilianiahuxleyi)metacaspases(EhMCs)为代表分析其结构和功能,依据Uniprot和String数据库挖掘与metacaspase功能相关并参与浮游植物PCD过程的互作蛋白。结果发现:共计29条、四种类型的metacaspases分布于11种浮游植物中,它们的metacaspase蛋白一级结构存在明显差异,但位于p20的组氨酸和半胱氨酸活性位点均具有高度的保守性;EhMCs三级结构与酵母的metacaspase十分相似,暗示它们具有类似的功能;浮游植物metacaspases与多种蛋白存在直接或间接的互作关系,在细胞PCD过程、响应环境胁迫和能量代谢等方面发挥重要作用。另外.metacaspase的磷酸化修饰可能在浮游植物PCD信号转导过程中起调节作用。     

日本七鳃鳗外周血细胞显微结构及类淋巴细胞体外培养

以日本七鳃鳗(Lampetra japonica)为研究对象,观察其外周血细胞显微结构和探讨类淋巴细胞原代培养条件。采用Wright氏和Giemsa氏染色法对日本七鳃鳗外周血液有形成分进行显微观察,可鉴别出红细胞、淋巴细胞、中性粒细胞、单核细胞、嗜酸性粒细胞;未发现嗜碱性粒细胞。通过Ficoll密度梯度离心法和流式细胞仪分选获得高纯度的七鳃鳗类淋巴细胞。经条件优化后确定最适培养液L15+20%FBS(5%七鳃鳗血清)+0.46%NaCl,在18℃,pH 6.8-7.0的条件下对日本七鳃鳗类淋巴细胞进行体外培养,大多数类淋巴细胞半贴壁生长,细胞状态较好,最长可以存活近一个月。类淋巴细胞的原代培养为日本七鳃鳗细胞体外培养的深入研究提供了实验依据。     

Influence of natural fractures on gas accumulation in the Upper Triassic tight gas sandstones in the northwestern Sichuan Basin,China

The Upper Triassic Xujiahe Formation in the northwestern Sichuan Basin, China, is a typical tight gas sandstone reservoir that contains natural fractures and has an average porosity of 1.10% and air permeability less than 0.1 md because of compaction and cementation. According to outcrops, cores and image logs, three types of natural fractures, namely, tectonic, diagenetic and overpressure-related fractures, have developed in the tight gas sandstones. The tectonic fractures include small faults, intraformational shear fractures and horizontal shear fractures, whereas the diagenetic fractures mainly include bed-parallel fractures. According to thin sections, the microfractures also include tectonic, diagenetic and overpressure-related microfractures. The diagenetic microfractures consist of transgranular, intragranular and grain-boundary fractures. Among these fractures, intraformational shear fractures, horizontal shear fractures and small faults are predominant and significant for fluid movement. Based on the Monte Carlo method, these intraformational shear fractures and horizontal shear fractures improve the reservoir porosity and permeability, thus serving as an important storage space and primary fluid-flow channels in the tight sandstones. The small faults may provide seepage channels in adjacent layers by cutting through layers. In addition, these intragranular and grain-boundary fractures increase the connectivity of the tight gas sandstones by linking tiny pores. The tectonic microfractures improve the seepage capability of the tight gas sandstones to some extent. Low-dip angle fractures are more abundant in the T₃X³ member than in the T₃X² and T₃X⁴ members. The fracture intensities of the sandstones in the T₃X³ member are greater than those in the T₃X² and T₃X⁴ members. The fracture intensities do not always decrease with increasing bed thickness for the tight sandstones. When the bed thickness of the tight sandstones is less than 1.0 m, the fracture intensities increase with increasing bed thickness in the T₃X³ member. Fluid inclusion evidence and burial history analysis indicate that the tectonic fractures developed over three periods. The first period was at the end of the Triassic to the Early Jurassic. The tectonic fractures developed during oil generation but before the matrix's porosity and permeability reduced, which suggests that these tectonic fractures could provide seepage channels for oil migration and accumulation. The second period was at the end of the Cretaceous after the matrix's porosity and permeability reduced but during peak gas generation, which indicates that gas mainly migrated and accumulated in the tectonic fractures. The third period was at the end of the Eogene to the Early Neogene. The tectonic fractures could provide seepage channels for secondary gas migration and accumulation from the Upper Triassic Xujiahe Formation into the overlying Jurassic Formation.     

Fluid sources and stable isotope signatures in authigenic carbonates from the Northern Apennines,Italy

Authigenic carbonates are frequently associated with methane cold-seep systems, which extensively occur in various geologic settings worldwide. Of interest is the relation between the fluids involved in their formation and the isotopic signals recorded in the carbonate cements. Along the Northern Apennines foothills (Italy), hydrocarbons and connate waters still seeping nowadays are believed to be the primary sources for the formation of fossil authigenic carbonate found in Plio-Pleistocene marine sediments. Four selected outcrops of dolomitic authigenic carbonates were analysed to compare signature of seeping fluids with fractionation of stable carbon and oxygen isotopes recorded in the carbonate.Along the foothills, deep methane-rich fluids spontaneously rise to the surface through mud volcanoes or are exploited in wells drilled nearby to the fossil Plio-Pleistocene authigenic carbonates. The plumbing system providing fluids to present-day cold seeps was structurally achieved in Late Miocene and Plio-Pleistocene. δ¹³C values of methane, which vary from −51.9 to −43.0‰ VPDB, indicate that gas composition from the deep hydrocarbon reservoirs is relatively uniform along the foothills. On the contrary, δ¹³C in fossil authigenic carbonates strongly varies among different areas and also within the same outcrop.The different carbon sources that fed the investigated carbonates were identified and include: thermogenic methane from the deep Miocene reservoirs, ¹³C-enriched CO₂ derived from secondary methanogenesis and microbial methane from Pliocene successions buried in the Po Plain. The δ¹³C variability documented among samples from a single outcrop testifies that the authigenic carbonates might represent a record of varying biogeochemical processes in the hydrocarbon reservoirs. The sources of stable oxygen isotopes in authigenic carbonates are often ascribed to marine water. Oxygen isotopic fractionation in the dolomite cements indicates that marine pore water couldn't be the sole source of oxygen. δ¹⁸O values provide a preliminary evidence that connate waters had a role in the carbonates precipitation. The concomitant occurrence of active cold seepages and fossil record of former plumbing systems suggests that generation and migration of hydrocarbons are long-lasting and very effective processes along the Northern Apennines foothills.     

Numerical modeling study of mineralization induced by methane cold seep at the sea bottom

In order to investigate the response of authigenic minerals to gas hydrate geo-systems, the biogeochemical processes and its induced mineralization were predicted by employing the comprehensive reactive transport modeling approach. Based on the available data extracted from the northern continental slope area of the South China Sea, a 1-D vertical column model was developed. Three cases with different upward methane flux rates and three cases with different mineral compositions, i.e., a total of six cases were designed to investigate the effects of variations in the depth of sulfate methane transition zone (SMTZ) and in the mineral composition on the formation of authigenic minerals. The simulation results indicate that the SMTZ depth influenced by both the upward methane flux rate and the initial composition played an important role in the formation of authigenic minerals. The AOM reaction is intensive at the interface, and the precipitation amount of calcite is large, which is mainly controlled by AOM. When the methane leakage rate is 20 times higher than the base case, aragonite starts to precipitate. During the simulation, oligoclase, k-feldspar, smectite-Na, smectite-Ca, chlorite dissolved. Our study specific to this area as a starting point may provide a quantitative approach for investigating carbonate and pyrite formation in hydrate-bearing sediments accounting for methane oxidation and sulfate reduction. The method presented here and the model built in this study can be used for other sites with similar conditions. In addition, this study may serve as an indication for the potential natural gas hydrate reservoir in depth, and is also significant for marine carbon and sulfur cycle.