台湾海峡金色小沙丁鱼幼鱼索饵群体研究

本文研究了台湾海峡南部和北部的金色小沙丁鱼当年生幼鱼计数性状和量度性状特征差异,并结合其生物学特性、产卵场、洄游及海区环境特征进行综合分析。结果表明,台湾海峡南部和北部的索饵幼鱼仍属于同一地方种群:闽南-台湾浅滩语地方种群。根据其索饵洄游特点,可分为台湾海峡南部和北部两个索饵群体.     

油田水动力系统与油气藏的形成

油田水中存在“沉积承压水”和“地表渗入水”两个相对独立而又相互作用的水动力系统,前者由上覆地层重量所引起的地静压力以及地静压力等引起的异常孔隙流体压力所形成,具有内循环承压式水交替特征,后者为大气降水和地表水向储集层的渗入而形成,以外循环渗入或水交替为特征。这两大系统（尤其是前者）的作用在很大程度上控制了含油气盆地中油气的运移、聚集和保存,油气聚集区主要位于水文地质带中的交管阻滞一停滞带内,沉积交替强度的低值区是油气富集区,沉积水的离心流使凹陷中的油气围绕凹陷中心呈带状分布。     

利用牙鲆鳃细胞系分离和培养淋巴囊肿病毒

本文利用牙鲆鳃细胞系进行了养殖牙鲆淋巴囊肿病毒的分离及培养 ,并通过电镜对培养细胞中淋巴囊肿病毒的形态及感染循环进行了初步研究。将病鱼的淋巴囊肿组织无菌滤液接种牙鲆细胞系 ,细胞出现了明显的细胞病变 ( Cytopathic effect,CPE)。电镜观察在培养细胞的胞质中有病毒的包涵体 ,胞质中散在 6角形、5角形或圆形的病毒粒子 ,大小为 10 0～ 140 nm之间。在感染细胞的线粒体中也存在大量的病毒颗粒。     

二色桌片参酶解液对转化细胞的效应

用二色桌片参（Mensamariaintercedens）酶解液处理转化的人胚肺成纤维细胞（NLF）后，细胞生长受到显著抑制，抑制率达64．84％，光镜下细胞透光性加强，铺展较好，细胞核形更为规则，核畸形现象减少；线粒体结构趋于正常；软琼脂集落实验表明，细胞集落形成率由23ｘ10－5下降至12．5ｘ10－5，裸小鼠致瘤实验显示抑瘤率为82．39％，差异显著（p＜5％）。结果提示，二色桌片参酶解液可能逆转转化的HLF细胞的恶性表型，对其有一定的诱导分化作用。     

高精度CTD剖面仪电导率传感器的研究和实验

高精度CTD剖面仪是海洋863项目的重要组成部分，是海洋调查和水文观测必不可少的仪器，本文详细论述了CTD剖面仪中电导率传感器的研制方法和过程，并给出传感器现已达到的水平，提出还需要进一步进行的工作和深入研究的问题。     

Application of Atomic Force Microscopy to Observation of Marine Bacteria

The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as a new tool to study the fine structures of marine bacteria. This revised version was published online in July 2006 with corrections to the Cover Date.     

用含氯化镉培养液培养塔胞藻的生物学效应

在塔胞藻培养液中加入不同浓度的CdCl2,然后分析了培养在各种处理和对照组的塔胞藻后代.结果发现,随着镉离子浓度的升高,细胞的生长繁殖量和体积在前2轮(9d为一轮)培养中均明显降低,而在第3轮培养时,细胞的繁殖和大小接近常态.随后对处理后多种细胞内含物含量进行了分析,显示出:随着培养液中镉离子浓度增高,细胞内的蛋白质、叶绿素含量均明显下降,而可溶性糖、核酸的含量与对照相比变化不大.